Restriction map for
pFN21AB7745
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BciVI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoT22I FseI FspI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluI MroI MunI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tsp509I Tth111I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 898 GTCTAC GT^[AC][GT]AC
AccII 60 CGCG CG^CG
AfaI 144 GTAC GT^AC
AlwI 267 GGATC GGATC(4/5)
AseI 1306 ATTAAT AT^TAAT
AsnI 1306 ATTAAT AT^TAAT
AspHI 876 GAGCAC G[AT]GC[AT]^C
BalI 82 TGGCCA TGG^CCA
BclI 1199 TGATCA T^GATCA
BfaI 1016 CTAG C^TAG
BglI 802 GCCGTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI 29 CGGCCG CG[GA][CT]^CG
BsiHKAI 876 GAGCAC G[AT]GC[AT]^C
BsmAI 825 GAGAC GTCTC(1/5)
BstUI 60 CGCG CG^CG
BstXI 518 CCAACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 267 GGATCT [GA]^GATC[CT]
DsaI 201 CCACGG C^C[GA][CT]GG
EagI 29 CGGCCG C^GGCCG
EarI 1273 CTCTTC CTCTTC(1/4)
EclXI 29 CGGCCG C^GGCCG
Eco52I 29 CGGCCG C^GGCCG
EcoNI 711 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 760 GATATC GAT^ATC
FbaI 1199 TGATCA T^GATCA
HgaI 190 GACGC GACGC(5/10)
Ksp632I 1273 CTCTTC CTCTTC(1/4)
MaeI 1016 CTAG C^TAG
MaeIII 748 GTGAC ^GT[ACGT]AC
MflI 267 GGATCT [GA]^GATC[CT]
MluNI 82 TGGCCA TGG^CCA
MscI 82 TGGCCA TGG^CCA
MvnI 60 CGCG CG^CG
NlaIII 1023 CATG CATG^
PshBI 1306 ATTAAT AT^TAAT
PvuII 625 CAGCTG CAG^CTG
RsaI 144 GTAC GT^AC
SmlI 871 CTCAAG C^T[CT][GA]AG
StuI 665 AGGCCT AGG^CCT
Tsp45I 748 GTGAC ^GT[GC]AC
XhoII 267 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
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ApaI 10 GGGCCC GGGCC^C
886 GGGCCC
BbeI 156 GGCGCC GGCGC^C
171 GGCGCC
BbsI 378 GAAGAC GAAGAC(2/6)
595 GAAGAC
BbvCI 93 CCTCAGC CCTCAGC(-5/-2)
475 GCTGAGG
BcgI 47 CGAAGGTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
179 GCACCTGCCTCG
BpmI 271 CTGGAG CTGGAG(16/14)
1123 CTGGAG
BpuAI 378 GAAGAC GAAGAC(2/6)
595 GAAGAC
BseRI 913 GAGGAG GAGGAG(10/8)
943 GAGGAG
BspMI 181 ACCTGC ACCTGC(4/8)
857 ACCTGC
KasI 156 GGCGCC G^GCGCC
171 GGCGCC
MaeII 488 ACGT A^CGT
521 ACGT
MseI 782 TTAA T^TAA
1307 TTAA
MslI 327 CATCAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
516 CACCAACGTG
NarI 156 GGCGCC GG^CGCC
171 GGCGCC
PpuMI 452 GGGTCCT [GA]G^G[AT]CC[CT]
1295 GGGACCT
PstI 859 CTGCAG CTGCA^G
1144 CTGCAG
SfcI 859 CTGCAG C^T[GA][CT]AG
1144 CTGCAG
SfoI 156 GGCGCC GGC^GCC
171 GGCGCC
SmaI 14 CCCGGG CCC^GGG
370 CCCGGG
Tru9I 782 TTAA T^TAA
1307 TTAA
XcmI 254 CCAAAAGGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
728 CCAACAAGAGGCTGG
XmaCI 14 CCCGGG C^CCGGG
370 CCCGGG
XmaI 14 CCCGGG C^CCGGG
370 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcyI AluI AlwNI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BsaHI BsaJI BsgI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MboI MboII
MnlI MspA1I MspI MvaI MwoI
NaeI NciI NdeII NgoMIV NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI TspRI TthHB8I