(Circular) (Six-base) MAPSORT of: pFN21AB7745.seq Check: 5365 from: 1 to: 6016 pFN21AB7745 6016 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2195 2195 Size: 6016 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3826 278 Size: 53 83 186 936 2290 2468 Fragments arranged by size: 2468 2290 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2904 3359 1937 Size: 967 455 4594 Fragments arranged by size: 4594 967 455 Acc65I G'GTAC_C Cuts at: 3337 3337 Size: 6016 AclI AA'CG_TT Cuts at: 1563 4143 4516 1563 Size: 2580 373 3063 Fragments arranged by size: 3063 2580 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2666 4079 5463 5731 1632 Size: 1034 1413 1384 268 1917 Fragments arranged by size: 1917 1413 1384 1034 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5819 AflIII A'CryG_T Cuts at: 1203 4915 5892 1203 Size: 3712 977 1327 Fragments arranged by size: 3712 1327 977 AgeI A'CCGG_T Cuts at: 4847 4847 Size: 6016 AhdI GACnn_n'nnGTC Cuts at: 4745 4745 Size: 6016 AleI CACnn'nnGTG Cuts at: 1166 2525 1166 Size: 1359 4657 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6016 AlwNI CAG_nnn'CTG Cuts at: 2126 2644 3172 3759 5331 2126 Size: 518 528 587 1572 2811 Fragments arranged by size: 2811 1572 587 528 518 ApaI G_GGCC'C Cuts at: 2019 2895 2019 Size: 876 5140 ApaLI G'TGCA_C Cuts at: 4073 5229 4073 Size: 1156 4860 ApoI r'AATT_y Cuts at: 1493 3325 3551 3703 5812 1493 Size: 1832 226 152 2109 1697 Fragments arranged by size: 2109 1832 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 3312 4570 160 Size: 872 2280 1258 1606 Fragments arranged by size: 2280 1606 1258 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6016 AvaI C'yCGr_G Cuts at: 1870 1943 2019 2213 2375 3341 1870 Size: 73 76 194 162 966 4545 Fragments arranged by size: 4545 966 194 162 76 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2132 2165 2132 Size: 33 5983 BamHI G'GATC_C Cuts at: 3346 3713 3346 Size: 367 5649 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2059 2161 2176 2183 3337 Size: 325 217 627 272 102 15 7 1154 Cuts at: 3337 4792 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1154 627 325 272 217 102 15 7 BanII G_rGCy'C Cuts at: 1391 2019 2075 2895 3036 3335 1391 Size: 628 56 820 141 299 4072 Fragments arranged by size: 4072 820 628 299 141 56 BbeI G_GCGC'C Cuts at: 2165 2180 2165 Size: 15 6001 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2390 2607 928 Size: 971 491 217 4337 Fragments arranged by size: 4337 971 491 217 BbvCI CC'TCA_GC Cuts at: 2099 2481 2099 Size: 382 5634 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2041 2075 2173 2207 4207 Size: 34 50 34 20 34 98 34 2000 Cuts at: 4207 4241 1903 Size: 34 3678 Fragments arranged by size: 3678 2000 98 50 34 34 34 34 34 20 BciVI GTATCCnnnnn_n' Cuts at: 3908 5118 3908 Size: 1210 4806 BclI T'GATC_A Cuts at: 1548 3204 1548 Size: 1656 4360 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2813 4627 136 Size: 107 122 71 631 605 1141 1814 1525 Fragments arranged by size: 1814 1525 1141 631 605 122 107 71 BglII A'GATC_T Cuts at: 6011 6011 Size: 6016 BlpI GC'TnA_GC Cuts at: 3433 3433 Size: 6016 Bme1580I G_kGCm'C Cuts at: 1790 2019 2064 2844 2895 2915 4077 5233 1790 Size: 229 45 780 51 20 1162 1156 2573 Fragments arranged by size: 2573 1162 1156 780 229 51 45 20 BmgBI CAC'GTC Cuts at: 1318 2494 1318 Size: 1176 4840 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2482 3747 4705 Size: 416 53 83 399 24 1065 1265 958 Cuts at: 4705 442 Size: 1753 Fragments arranged by size: 1753 1265 1065 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6016 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2715 2715 Size: 6016 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2297 3149 4676 1239 Size: 1058 852 1527 2579 Fragments arranged by size: 2579 1527 1058 852 Bpu10I CC'TnA_GC Cuts at: 2099 2438 2481 2099 Size: 339 43 5634 Fragments arranged by size: 5634 339 43 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2861 4141 5006 5304 2861 Size: 1280 865 298 3573 Fragments arranged by size: 3573 1280 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4679 882 Size: 617 3180 2219 Fragments arranged by size: 3180 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3807 493 Size: 711 2603 2702 Fragments arranged by size: 2702 2603 711 BsaBI GATnn'nnATC Cuts at: 6010 6010 Size: 6016 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2162 2177 2196 3823 Size: 53 83 186 936 629 15 19 1627 Cuts at: 3823 4205 4840 275 Size: 382 635 1451 Fragments arranged by size: 1627 1451 936 635 629 382 186 83 53 19 15 BsaWI w'CCGG_w Cuts at: 4449 4847 5121 5268 5679 4449 Size: 398 274 147 411 4786 Fragments arranged by size: 4786 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2149 2179 1178 Size: 30 165 30 746 30 5015 Fragments arranged by size: 5015 746 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2933 2963 1186 Size: 1747 30 4239 Fragments arranged by size: 4239 1747 30 BseYI C'CCAG_C Cuts at: 2039 2109 2119 2351 2404 2632 2981 2997 3002 Size: 70 10 232 53 228 349 16 5 Cuts at: 3002 3072 5219 5715 2039 Size: 70 2147 496 2340 Fragments arranged by size: 2340 2147 496 349 232 228 70 70 53 16 10 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2516 2945 3211 1163 Size: 573 780 429 266 3968 Fragments arranged by size: 3968 780 573 429 266 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2037 3466 4227 5255 1540 Size: 275 186 36 1429 761 1028 2301 Fragments arranged by size: 2301 1429 1028 761 275 186 36 BsiHKAI G_wGCw'C Cuts at: 2885 4077 4162 5233 2885 Size: 1192 85 1071 3668 Fragments arranged by size: 3668 1192 1071 85 BsmI GAATG_Cn' Cuts at: 3527 3620 3527 Size: 93 5923 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2019 2064 2075 2844 2885 2895 2915 Size: 399 229 45 11 769 41 10 20 Cuts at: 2915 3036 3335 4077 4162 5233 1391 Size: 121 299 742 85 1071 2174 Fragments arranged by size: 2174 1071 769 742 399 299 229 121 85 45 41 20 11 10 BspEI T'CCGG_A Cuts at: 5679 5679 Size: 6016 BspHI T'CATG_A Cuts at: 3905 5635 3905 Size: 1730 4286 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2195 2871 3371 844 Size: 1351 676 500 3489 Fragments arranged by size: 3489 1351 676 500 BsrBI CCG'CTC Cuts at: 1394 1823 3903 1394 Size: 429 2080 3507 Fragments arranged by size: 3507 2080 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4511 4685 65 Size: 1183 3263 174 1396 Fragments arranged by size: 3263 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2239 2247 2254 4660 4847 1700 Size: 110 429 8 7 2406 187 2869 Fragments arranged by size: 2869 2406 429 187 110 8 7 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6016 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6016 BssSI C'ACGA_G Cuts at: 4076 5088 4076 Size: 1012 5004 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6016 BstBI TT'CG_AA Cuts at: 4820 4820 Size: 6016 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6016 BstXI CCAn_nnnn'nTGG Cuts at: 2530 2530 Size: 6016 BstYI r'GATC_y Cuts at: 1972 2272 3346 3713 4098 4115 5567 5653 5665 Size: 300 1074 367 385 17 1452 86 12 Cuts at: 5665 6011 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1074 385 367 346 300 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2206 513 Size: 552 1141 4323 Fragments arranged by size: 4323 1141 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2081 2245 487 Size: 683 56 855 164 4258 Fragments arranged by size: 4258 855 683 164 56 BtsI GCAGTG_nn' Cuts at: 3532 4326 4346 3532 Size: 794 20 5202 Fragments arranged by size: 5202 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6016 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5981 DraI TTT'AAA Cuts at: 3320 3320 Size: 6016 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5023 809 Size: 507 3707 1802 Fragments arranged by size: 3707 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2087 2256 Size: 54 1425 50 275 18 204 53 169 Cuts at: 2256 3463 4352 5981 8 Size: 1207 889 1629 43 Fragments arranged by size: 1629 1425 1207 889 275 204 169 54 53 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2034 3463 1537 Size: 275 222 1429 4090 Fragments arranged by size: 4090 1429 275 222 EarI CTCTTCn'nnn_ Cuts at: 1353 3284 3946 1353 Size: 1931 662 3423 Fragments arranged by size: 3423 1931 662 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4603 4977 5123 387 Size: 1064 138 3014 374 146 1280 Fragments arranged by size: 3014 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2297 2666 3149 4079 4676 5463 5731 Size: 393 665 369 483 930 597 787 268 Cuts at: 5731 1239 Size: 1524 Fragments arranged by size: 1524 930 787 665 597 483 393 369 268 EcoNI CCTnn'n_nnAGG Cuts at: 2720 2720 Size: 6016 EcoO109I rG'GnC_Cy Cuts at: 2015 2016 2458 2815 2891 3079 3301 2015 Size: 1 442 357 76 188 222 4730 Fragments arranged by size: 4730 442 357 222 188 76 1 EcoRI G'AATT_C Cuts at: 3325 3703 3325 Size: 378 5638 EcoRV GAT'ATC Cuts at: 2767 2767 Size: 6016 FspI TGC'GCA Cuts at: 4522 4522 Size: 6016 HaeII r_GCGC'y Cuts at: 1678 2165 2180 3065 5163 1678 Size: 487 15 885 2098 2531 Fragments arranged by size: 2531 2098 885 487 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2149 2181 4657 4689 4731 Size: 32 135 32 577 32 2476 32 42 Cuts at: 4731 4763 1373 Size: 32 2626 Fragments arranged by size: 2626 2476 577 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3360 3606 669 Size: 988 281 1422 246 3079 Fragments arranged by size: 3079 1422 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6016 HpaI GTT'AAC Cuts at: 3606 3606 Size: 6016 KasI G'GCGC_C Cuts at: 2161 2176 2161 Size: 15 6001 KpnI G_GTAC'C Cuts at: 3341 3341 Size: 6016 MfeI C'AATT_G Cuts at: 3615 3615 Size: 6016 MluI A'CGCG_T Cuts at: 5892 5892 Size: 6016 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5130 5314 1305 Size: 3825 184 2007 Fragments arranged by size: 3825 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2089 10 Size: 54 1425 343 257 3937 Fragments arranged by size: 3937 1425 343 257 54 MslI CAynn'nnrTG Cuts at: 518 1166 2336 2525 3974 4333 4492 518 Size: 648 1170 189 1449 359 159 2042 Fragments arranged by size: 2042 1449 1170 648 359 189 159 MspA1I CmG'CkG Cuts at: 1606 2039 2146 2174 2480 2632 3432 4109 5257 Size: 433 107 28 306 152 800 677 1148 Cuts at: 5257 5502 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 800 677 433 306 245 152 107 28 NaeI GCC'GGC Cuts at: 2241 2249 2256 2241 Size: 8 7 6001 Fragments arranged by size: 6001 8 7 NarI GG'CG_CC Cuts at: 2162 2177 2162 Size: 15 6001 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5464 NdeI CA'TA_TG Cuts at: 387 387 Size: 6016 NgoMIV G'CCGG_C Cuts at: 2239 2247 2254 2239 Size: 8 7 6001 Fragments arranged by size: 6001 8 7 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6016 NotI GC'GGCC_GC Cuts at: 3463 3463 Size: 6016 NspI r_CATG'y Cuts at: 1132 1229 3374 4919 1132 Size: 97 2145 1545 2229 Fragments arranged by size: 2229 2145 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6016 PciI A'CATG_T Cuts at: 4915 4915 Size: 6016 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6016 PfoI T'CCnGG_A Cuts at: 2460 2460 Size: 6016 PmeI GTTT'AAAC Cuts at: 3320 3320 Size: 6016 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3152 3184 4085 4117 5598 5630 3152 Size: 32 901 32 1481 32 3538 Fragments arranged by size: 3538 1481 901 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2458 3301 2458 Size: 843 5173 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6016 PsiI TTA'TAA Cuts at: 3586 3586 Size: 6016 PspOMI G'GGCC_C Cuts at: 2015 2891 2015 Size: 876 5140 PstI C_TGCA'G Cuts at: 830 2868 3153 3368 830 Size: 2038 285 215 3478 Fragments arranged by size: 3478 2038 285 215 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6016 PvuII CAG'CTG Cuts at: 2632 2632 Size: 6016 SalI G'TCGA_C Cuts at: 1936 3358 1936 Size: 1422 4594 SbfI CC_TGCA'GG Cuts at: 3368 3368 Size: 6016 ScaI AGT'ACT Cuts at: 4264 5976 4264 Size: 1712 4304 SfcI C'TryA_G Cuts at: 826 1045 2864 3149 3364 4499 5180 5371 826 Size: 219 1819 285 215 1135 681 191 1471 Fragments arranged by size: 1819 1471 1135 681 285 219 215 191 SfoI GGC'GCC Cuts at: 2163 2178 2163 Size: 15 6001 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6016 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6016 SmaI CCC'GGG Cuts at: 1872 2021 2377 3343 1872 Size: 149 356 966 4545 Fragments arranged by size: 4545 966 356 149 SmlI C'TyrA_G Cuts at: 820 1017 1943 2876 4120 5021 5283 820 Size: 197 926 933 1244 901 262 1553 Fragments arranged by size: 1553 1244 933 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3807 493 Size: 3314 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6016 SphI G_CATG'C Cuts at: 1132 3374 1132 Size: 2242 3774 SspI AAT'ATT Cuts at: 5 52 3940 5 Size: 47 3888 2081 Fragments arranged by size: 3888 2081 47 StuI AGG'CCT Cuts at: 1517 2672 1517 Size: 1155 4861 StyI C'CwwG_G Cuts at: 513 1065 2025 2319 2673 2887 3105 3222 3295 Size: 552 960 294 354 214 218 117 73 Cuts at: 3295 3455 513 Size: 160 3074 Fragments arranged by size: 3074 960 552 354 294 218 214 160 117 73 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2900 3156 4043 4060 4213 4398 4843 Size: 1255 1320 256 887 17 153 185 445 Cuts at: 4843 325 Size: 1498 Fragments arranged by size: 1498 1320 1255 887 445 256 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4262 5974 96 Size: 274 80 33 51 1443 2285 1712 138 Fragments arranged by size: 2285 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5976 6008 5976 Size: 32 5984 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6016 XbaI T'CTAG_A Cuts at: 3352 3352 Size: 6016 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2266 2740 2266 Size: 474 5542 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6016 XmaI C'CCGG_G Cuts at: 1870 2019 2375 3341 1870 Size: 149 356 966 4545 Fragments arranged by size: 4545 966 356 149 XmnI GAAnn'nnTTC Cuts at: 4145 4145 Size: 6016 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3824 276 Size: 53 83 186 936 2290 2468 Fragments arranged by size: 2468 2290 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI