Restriction map for
pFN21AB7745
(Linear) (Six-base) MAPSORT of: KIBB7745.seq Check: 6053 from: 1 to: 1311
KIBB7745.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 190 1311
Size: 190 1121
AccI GT'mk_AC
Cuts at: 0 899 1311
Size: 899 412
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 1311
Size: 661 650
AleI CACnn'nnGTG
Cuts at: 0 520 1311
Size: 520 791
AlwNI CAG_nnn'CTG
Cuts at: 0 121 639 1167 1311
Size: 121 518 528 144
Fragments arranged by size:
528 518 144 121
ApaI G_GGCC'C
Cuts at: 0 14 890 1311
Size: 14 876 421
Fragments arranged by size:
876 421 14
AseI AT'TA_AT
Cuts at: 0 1307 1311
Size: 1307 4
AvaI C'yCGr_G
Cuts at: 0 14 208 370 1311
Size: 14 194 162 941
Fragments arranged by size:
941 194 162 14
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 127 160 1311
Size: 127 33 1151
Fragments arranged by size:
1151 127 33
BanI G'GyrC_C
Cuts at: 0 54 156 171 178 1311
Size: 54 102 15 7 1133
Fragments arranged by size:
1133 102 54 15 7
BanII G_rGCy'C
Cuts at: 0 14 70 890 1031 1311
Size: 14 56 820 141 280
Fragments arranged by size:
820 280 141 56 14
BbeI G_GCGC'C
Cuts at: 0 160 175 1311
Size: 160 15 1136
Fragments arranged by size:
1136 160 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 385 602 1311
Size: 385 217 709
Fragments arranged by size:
709 385 217
BbvCI CC'TCA_GC
Cuts at: 0 94 476 1311
Size: 94 382 835
Fragments arranged by size:
835 382 94
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 36 70 168 202 1311
Size: 36 34 98 34 1109
Fragments arranged by size:
1109 98 36 34 34
BclI T'GATC_A
Cuts at: 0 1199 1311
Size: 1199 112
BglI GCCn_nnn'nGGC
Cuts at: 0 808 1311
Size: 808 503
Bme1580I G_kGCm'C
Cuts at: 0 14 59 839 890 910 1311
Size: 14 45 780 51 20 401
Fragments arranged by size:
780 401 51 45 20 14
BmgBI CAC'GTC
Cuts at: 0 489 1311
Size: 489 822
BmrI ACTGGGnnnn_n'
Cuts at: 0 477 1311
Size: 477 834
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 710 1311
Size: 710 601
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 1144 1311
Size: 292 852 167
Fragments arranged by size:
852 292 167
Bpu10I CC'TnA_GC
Cuts at: 0 94 433 476 1311
Size: 94 339 43 835
Fragments arranged by size:
835 339 94 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 856 1311
Size: 856 455
BsaHI Gr'CG_yC
Cuts at: 0 157 172 191 1311
Size: 157 15 19 1120
Fragments arranged by size:
1120 157 19 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 144 174 1311
Size: 144 30 1137
Fragments arranged by size:
1137 144 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 928 958 1311
Size: 928 30 353
Fragments arranged by size:
928 353 30
BseYI C'CCAG_C
Cuts at: 0 34 104 114 346 399 627 976 992
Size: 34 70 10 232 53 228 349 16
Cuts at: 992 997 1067 1311
Size: 5 70 244
Fragments arranged by size:
349 244 232 228 70 70 53 34
16 10 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 940 1206 1311
Size: 511 429 266 105
Fragments arranged by size:
511 429 266 105
BsiEI CG_ry'CG
Cuts at: 0 32 1311
Size: 32 1279
BsiHKAI G_wGCw'C
Cuts at: 0 880 1311
Size: 880 431
Bsp1286I G_dGCh'C
Cuts at: 0 14 59 70 839 880 890 910 1031
Size: 14 45 11 769 41 10 20 121
Cuts at: 1031 1311
Size: 280
Fragments arranged by size:
769 280 121 45 41 20 14 11
10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 190 866 1311
Size: 190 676 445
Fragments arranged by size:
676 445 190
BsrFI r'CCGG_y
Cuts at: 0 234 242 249 1311
Size: 234 8 7 1062
Fragments arranged by size:
1062 234 8 7
BstXI CCAn_nnnn'nTGG
Cuts at: 0 525 1311
Size: 525 786
BstYI r'GATC_y
Cuts at: 0 267 1311
Size: 267 1044
BtgI C'CryG_G
Cuts at: 0 201 1311
Size: 201 1110
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 76 240 1311
Size: 76 164 1071
Fragments arranged by size:
1071 164 76
EaeI y'GGCC_r
Cuts at: 0 29 82 251 1311
Size: 29 53 169 1060
Fragments arranged by size:
1060 169 53 29
EagI C'GGCC_G
Cuts at: 0 29 1311
Size: 29 1282
EarI CTCTTCn'nnn_
Cuts at: 0 1279 1311
Size: 1279 32
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 661 1144 1311
Size: 292 369 483 167
Fragments arranged by size:
483 369 292 167
EcoNI CCTnn'n_nnAGG
Cuts at: 0 715 1311
Size: 715 596
EcoO109I rG'GnC_Cy
Cuts at: 0 10 11 453 810 886 1074 1296 1311
Size: 10 1 442 357 76 188 222 15
Fragments arranged by size:
442 357 222 188 76 15 10 1
EcoRV GAT'ATC
Cuts at: 0 762 1311
Size: 762 549
HaeII r_GCGC'y
Cuts at: 0 160 175 1060 1311
Size: 160 15 885 251
Fragments arranged by size:
885 251 160 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 144 176 1311
Size: 144 32 1135
Fragments arranged by size:
1135 144 32
KasI G'GCGC_C
Cuts at: 0 156 171 1311
Size: 156 15 1140
Fragments arranged by size:
1140 156 15
MscI TGG'CCA
Cuts at: 0 84 1311
Size: 84 1227
MslI CAynn'nnrTG
Cuts at: 0 331 520 1311
Size: 331 189 791
Fragments arranged by size:
791 331 189
MspA1I CmG'CkG
Cuts at: 0 34 141 169 475 627 1311
Size: 34 107 28 306 152 684
Fragments arranged by size:
684 306 152 107 34 28
NaeI GCC'GGC
Cuts at: 0 236 244 251 1311
Size: 236 8 7 1060
Fragments arranged by size:
1060 236 8 7
NarI GG'CG_CC
Cuts at: 0 157 172 1311
Size: 157 15 1139
Fragments arranged by size:
1139 157 15
NgoMIV G'CCGG_C
Cuts at: 0 234 242 249 1311
Size: 234 8 7 1062
Fragments arranged by size:
1062 234 8 7
PfoI T'CCnGG_A
Cuts at: 0 455 1311
Size: 455 856
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1147 1179 1311
Size: 1147 32 132
Fragments arranged by size:
1147 132 32
PpuMI rG'GwC_Cy
Cuts at: 0 453 1296 1311
Size: 453 843 15
Fragments arranged by size:
843 453 15
PspOMI G'GGCC_C
Cuts at: 0 10 886 1311
Size: 10 876 425
Fragments arranged by size:
876 425 10
PstI C_TGCA'G
Cuts at: 0 863 1148 1311
Size: 863 285 163
Fragments arranged by size:
863 285 163
PvuII CAG'CTG
Cuts at: 0 627 1311
Size: 627 684
SfcI C'TryA_G
Cuts at: 0 859 1144 1311
Size: 859 285 167
Fragments arranged by size:
859 285 167
SfoI GGC'GCC
Cuts at: 0 158 173 1311
Size: 158 15 1138
Fragments arranged by size:
1138 158 15
SmaI CCC'GGG
Cuts at: 0 16 372 1311
Size: 16 356 939
Fragments arranged by size:
939 356 16
SmlI C'TyrA_G
Cuts at: 0 871 1311
Size: 871 440
StuI AGG'CCT
Cuts at: 0 667 1311
Size: 667 644
StyI C'CwwG_G
Cuts at: 0 20 314 668 882 1100 1217 1290 1311
Size: 20 294 354 214 218 117 73 21
Fragments arranged by size:
354 294 218 214 117 73 21 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 895 1151 1311
Size: 895 256 160
Fragments arranged by size:
895 256 160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 261 735 1311
Size: 261 474 576
Fragments arranged by size:
576 474 261
XmaI C'CCGG_G
Cuts at: 0 14 370 1311
Size: 14 356 941
Fragments arranged by size:
941 356 14
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI ApaI AseI AvaI
BaeI BanI BanII BbeI BbsI BbvCI BcgI BclI
BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrFI BstXI BstYI BtgI BtgZI EaeI EagI
EarI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I KasI
MscI MslI MspA1I NaeI NarI NgoMIV PfoI PpiI
PpuMI PspOMI PstI PvuII SfcI SfoI SmaI SmlI
StuI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AsiSI AvrII BamHI
BciVI BfrBI BglII BlpI BmtI BsaI BsaAI BsaBI
BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtsI ClaI CspCI DraI DraIII DrdI EciI EcoICRI
EcoRI FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TatI TstI Tth111I XbaI XhoI XmnI ZraI