Restriction map for
pFN21AB7740
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1286I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstXI BstYI BstZ17I CelII
Cfr10I ClaI CpoI DraIII DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HaeIII HapII Hin1I HpaII KasI
KpnI KspI MaeI MamI MflI
MluI MluNI MroI MscI MspI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SspI StuI StyI
SwaI Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccI 575 GTCGAC GT^[AC][GT]AC
AlwI 389 GGATC GGATC(4/5)
AlwNI 145 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 826 CCTCAGG CC^T[ACGT]AGG
BciVI 436 GGATAC GTATCC(6/5)
BpmI 1205 CTCCAG CTGGAG(16/14)
BseRI 1168 GAGGAG GAGGAG(10/8)
BspMI 130 GCAGGT ACCTGC(4/8)
BsrDI 179 GCAATG GCAATG(2/0)
BstBI 1085 TTCGAA TT^CGAA
Bsu36I 826 CCTCAGG CC^T[ACGT]AGG
CfoI 374 GCGC GCG^C
DraI 849 TTTAAA TTT^AAA
DraII 132 AGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 732 GACAACAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 826 CCTCAGG CC^T[ACGT]AGG
EcoO109I 132 AGGTCCT [GA]G^G[ACGT]CC[CT]
HgaI 376 GCGTC GACGC(5/10)
HhaI 374 GCGC GCG^C
HinP1I 374 GCGC G^CGC
HindIII 1246 AAGCTT A^AGCTT
HpaI 621 GTTAAC GTT^AAC
MaeII 1439 ACGT A^CGT
MfeI 1190 CAATTG C^AATTG
MunI 1190 CAATTG C^AATTG
NspV 1085 TTCGAA TT^CGAA
PpuMI 132 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 51 CTGCAG CTGCA^G
SalI 575 GTCGAC G^TCGAC
SfcI 51 CTGCAG C^T[GA][CT]AG
SfuI 1085 TTCGAA TT^CGAA
SmlI 1163 CTCAAG C^T[CT][GA]AG
SphI 672 GCATGC GCATG^C
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccII 102 CGCG CG^CG
375 CGCG
Asp700I 874 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1078 GAAAGAATTC
AvaII 133 GGTCC G^G[AT]CC
963 GGACC
BbsI 694 GAAGAC GAAGAC(2/6)
970 GAAGAC
BpuAI 694 GAAGAC GAAGAC(2/6)
970 GAAGAC
BsgI 866 GTGCAG GTGCAG(16/14)
1441 GTGCAG
BsmFI 141 GGGAC GGGAC(10/14)
1436 GGGAC
BsmI 613 GAATGC GAATGC(1/-1)
1059 GCATTC
BstUI 102 CGCG CG^CG
375 CGCG
Cfr13I 133 GGTCC G^G[ACGT]CC
963 GGACC
EarI 782 CTCTTC CTCTTC(1/4)
262 GAAGAG
FauI 100 CCCGC CCCGC(4/6)
365 CCCGC
HincII 575 GTCGAC GT[CT]^[GA]AC
621 GTTAAC
HindII 575 GTCGAC GT[CT]^[GA]AC
621 GTTAAC
Ksp632I 782 CTCTTC CTCTTC(1/4)
262 GAAGAG
MslI 78 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
442 CACTATAATG
MvnI 102 CGCG CG^CG
375 CGCG
MwoI 1000 GCGGTTGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1248 GCTTCAGGAGC
NlaIV 159 GGAACC GG[ACGT]^[ACGT]CC
1254 GGAGCC
NspI 551 ACATGC [GA]CATG^[CT]
672 GCATGC
Sau96I 133 GGTCC G^G[ACGT]CC
963 GGACC
TfiI 802 GATTC G^A[AT]TC
1290 GATTC
Tsp45I 710 GTGAC ^GT[GC]AC
724 GTGAC
XmnI 874 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1078 GAAAGAATTC
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
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AciI AcsI AfaI AluI ApoI
BbvI BsaJI BsiYI BslI BsmAI
Bsp1407I BsrGI BssKI BstF5I BstNI
Cac8I DdeI DpnII EcoRI EcoRII
Fnu4HI FokI HinfI HphI ItaI
MaeIII MboI MboII MnlI MseI
MspA1I MvaI NdeII NlaIII PleI
PvuII RsaI Sau3AI ScrFI SfaNI
SspBI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I