(Circular) (Six-base) MAPSORT of: pFN21AB7740.seq Check: 444 from: 1 to: 6151 pFN21AB7740 6151 bp With 182 enzymes: SgfI * July 14, 2009 10:07 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3961 278 Size: 53 83 186 936 2425 2468 Fragments arranged by size: 2468 2425 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2581 3494 1937 Size: 644 913 4594 Fragments arranged by size: 4594 913 644 Acc65I G'GTAC_C Cuts at: 3472 3472 Size: 6151 AclI AA'CG_TT Cuts at: 1563 4278 4651 1563 Size: 2715 373 3063 Fragments arranged by size: 3063 2715 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2286 2470 3239 4214 5598 5866 1632 Size: 654 184 769 975 1384 268 1917 Fragments arranged by size: 1917 1384 975 769 654 268 184 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5954 AflIII A'CryG_T Cuts at: 1203 5050 6027 1203 Size: 3847 977 1327 Fragments arranged by size: 3847 1327 977 AgeI A'CCGG_T Cuts at: 4982 4982 Size: 6151 AhdI GACnn_n'nnGTC Cuts at: 4880 4880 Size: 6151 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6151 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6151 AlwNI CAG_nnn'CTG Cuts at: 2155 3894 5466 2155 Size: 1739 1572 2840 Fragments arranged by size: 2840 1739 1572 ApaLI G'TGCA_C Cuts at: 4208 5364 4208 Size: 1156 4995 ApoI r'AATT_y Cuts at: 1493 2634 2850 2864 2881 2933 3087 3460 3686 Size: 1141 216 14 17 52 154 373 226 Cuts at: 3686 3838 5947 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1141 373 226 216 154 152 52 17 14 AseI AT'TA_AT Cuts at: 160 1032 4705 160 Size: 872 3673 1606 Fragments arranged by size: 3673 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6151 AvaI C'yCGr_G Cuts at: 1870 1943 3476 1870 Size: 73 1533 4545 Fragments arranged by size: 4545 1533 73 BamHI G'GATC_C Cuts at: 3481 3848 3481 Size: 367 5784 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3472 4927 618 Size: 325 217 627 1685 1455 1842 Fragments arranged by size: 1842 1685 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3470 1391 Size: 2079 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2706 2982 928 Size: 971 807 276 4097 Fragments arranged by size: 4097 971 807 276 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4342 4376 1903 Size: 34 2405 34 3678 Fragments arranged by size: 3678 2405 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2435 4043 5253 2435 Size: 1608 1210 3333 Fragments arranged by size: 3333 1608 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6151 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4762 136 Size: 107 122 71 631 605 3090 1525 Fragments arranged by size: 3090 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6146 6146 Size: 6151 BlpI GC'TnA_GC Cuts at: 3568 3568 Size: 6151 Bme1580I G_kGCm'C Cuts at: 1790 4212 5368 1790 Size: 2422 1156 2573 Fragments arranged by size: 2573 2422 1156 BmgBI CAC'GTC Cuts at: 1318 3445 1318 Size: 2127 4024 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3882 4840 442 Size: 416 53 83 399 24 2465 958 1753 Fragments arranged by size: 2465 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6151 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2293 2847 2293 Size: 554 5597 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3195 4811 1239 Size: 1956 1616 2579 Fragments arranged by size: 2579 1956 1616 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3153 4276 5141 5439 3153 Size: 1123 865 298 3865 Fragments arranged by size: 3865 1123 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4814 882 Size: 617 3315 2219 Fragments arranged by size: 3315 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3942 493 Size: 711 2738 2702 Fragments arranged by size: 2738 2702 711 BsaBI GATnn'nnATC Cuts at: 6145 6145 Size: 6151 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3958 4340 4975 275 Size: 53 83 186 936 2425 382 635 1451 Fragments arranged by size: 2425 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4584 4982 5256 5403 5814 4584 Size: 398 274 147 411 4921 Fragments arranged by size: 4921 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5926 Fragments arranged by size: 5926 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3188 1186 Size: 2002 4149 BseYI C'CCAG_C Cuts at: 2256 5354 5850 2256 Size: 3098 496 2557 Fragments arranged by size: 3098 2557 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2892 3467 1163 Size: 573 1156 575 3847 Fragments arranged by size: 3847 1156 575 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3601 4362 5390 1540 Size: 275 186 1600 761 1028 2301 Fragments arranged by size: 2301 1600 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4212 4297 5368 4212 Size: 85 1071 4995 Fragments arranged by size: 4995 1071 85 BsmI GAATG_Cn' Cuts at: 2624 3064 3662 3755 2624 Size: 440 598 93 5020 Fragments arranged by size: 5020 598 440 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3470 4212 4297 5368 1391 Size: 399 1680 742 85 1071 2174 Fragments arranged by size: 2174 1680 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5814 5814 Size: 6151 BspHI T'CATG_A Cuts at: 4040 5770 4040 Size: 1730 4421 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2126 3439 3506 844 Size: 1282 1313 67 3489 Fragments arranged by size: 3489 1313 1282 67 BsrBI CCG'CTC Cuts at: 1394 1823 4038 1394 Size: 429 2215 3507 Fragments arranged by size: 3507 2215 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2191 4646 4820 65 Size: 1183 943 2455 174 1396 Fragments arranged by size: 2455 1396 1183 943 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4795 4982 1700 Size: 110 2985 187 2869 Fragments arranged by size: 2985 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2514 2553 2917 96 Size: 2418 39 364 3330 Fragments arranged by size: 3330 2418 364 39 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6151 BssSI C'ACGA_G Cuts at: 4211 5223 4211 Size: 1012 5139 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6151 BstBI TT'CG_AA Cuts at: 3091 4955 3091 Size: 1864 4287 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6151 BstYI r'GATC_y Cuts at: 1972 3481 3848 4233 4250 5702 5788 5800 6146 Size: 1509 367 385 17 1452 86 12 346 Cuts at: 6146 1972 Size: 1977 Fragments arranged by size: 1977 1509 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2832 2832 Size: 6151 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4660 Fragments arranged by size: 4660 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5412 Fragments arranged by size: 5412 683 56 BtsI GCAGTG_nn' Cuts at: 2053 3667 4461 4481 2053 Size: 1614 794 20 3723 Fragments arranged by size: 3723 1614 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6151 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6116 DraI TTT'AAA Cuts at: 2856 3455 2856 Size: 599 5552 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2743 5158 809 Size: 507 1427 2415 1802 Fragments arranged by size: 2415 1802 1427 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3598 4487 6116 Size: 54 1425 50 275 18 1768 889 1629 Cuts at: 6116 8 Size: 43 Fragments arranged by size: 1768 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3598 1537 Size: 275 1786 4090 Fragments arranged by size: 4090 1786 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2262 2793 4081 1353 Size: 909 531 1288 3423 Fragments arranged by size: 3423 1288 909 531 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4738 5112 5258 387 Size: 1064 138 3149 374 146 1280 Fragments arranged by size: 3149 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2286 2470 3195 3239 4214 4811 5598 Size: 393 654 184 725 44 975 597 787 Cuts at: 5598 5866 1239 Size: 268 1524 Fragments arranged by size: 1524 975 787 725 654 597 393 268 184 44 EcoO109I rG'GnC_Cy Cuts at: 2138 2138 Size: 6151 EcoRI G'AATT_C Cuts at: 2864 2933 3087 3460 3838 2864 Size: 69 154 373 378 5177 Fragments arranged by size: 5177 378 373 154 69 FspI TGC'GCA Cuts at: 4657 4657 Size: 6151 HaeII r_GCGC'y Cuts at: 1678 5298 1678 Size: 3620 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2420 2452 3349 3381 4792 Size: 32 135 32 848 32 897 32 1411 Cuts at: 4792 4824 4866 4898 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1411 897 848 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2582 2628 3495 3741 669 Size: 988 281 644 46 867 246 3079 Fragments arranged by size: 3079 988 867 644 281 246 46 HindIII A'AGCT_T Cuts at: 748 3251 748 Size: 2503 3648 HpaI GTT'AAC Cuts at: 2628 3741 2628 Size: 1113 5038 KpnI G_GTAC'C Cuts at: 3476 3476 Size: 6151 MfeI C'AATT_G Cuts at: 3195 3750 3195 Size: 555 5596 MluI A'CGCG_T Cuts at: 6027 6027 Size: 6151 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2262 5265 5449 1305 Size: 957 3003 184 2007 Fragments arranged by size: 3003 2007 957 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4329 Fragments arranged by size: 4329 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2087 2451 4109 4468 4627 518 Size: 648 843 78 364 1658 359 159 2042 Fragments arranged by size: 2042 1658 843 648 364 359 159 78 MspA1I CmG'CkG Cuts at: 1606 2061 2312 3032 3567 4244 5392 5637 1606 Size: 455 251 720 535 677 1148 245 2120 Fragments arranged by size: 2120 1148 720 677 535 455 251 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4660 Fragments arranged by size: 4660 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6151 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6151 NotI GC'GGCC_GC Cuts at: 3598 3598 Size: 6151 NspI r_CATG'y Cuts at: 1132 1229 2560 2681 3509 5054 1132 Size: 97 1331 121 828 1545 2229 Fragments arranged by size: 2229 1545 1331 828 121 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6151 PciI A'CATG_T Cuts at: 5050 5050 Size: 6151 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6151 PfoI T'CCnGG_A Cuts at: 2281 2281 Size: 6151 PmeI GTTT'AAAC Cuts at: 3455 3455 Size: 6151 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4220 4252 5733 5765 4220 Size: 32 1481 32 4606 Fragments arranged by size: 4606 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2138 2138 Size: 6151 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6151 PsiI TTA'TAA Cuts at: 3141 3721 3141 Size: 580 5571 PstI C_TGCA'G Cuts at: 830 2060 3503 830 Size: 1230 1443 3478 Fragments arranged by size: 3478 1443 1230 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6151 PvuII CAG'CTG Cuts at: 2061 2312 3032 2061 Size: 251 720 5180 Fragments arranged by size: 5180 720 251 SalI G'TCGA_C Cuts at: 1936 2580 3493 1936 Size: 644 913 4594 Fragments arranged by size: 4594 913 644 SbfI CC_TGCA'GG Cuts at: 3503 3503 Size: 6151 ScaI AGT'ACT Cuts at: 4399 6111 4399 Size: 1712 4439 SfcI C'TryA_G Cuts at: 826 1045 2056 3499 4634 5315 5506 826 Size: 219 1011 1443 1135 681 191 1471 Fragments arranged by size: 1471 1443 1135 1011 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6151 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6151 SmaI CCC'GGG Cuts at: 1872 3478 1872 Size: 1606 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3168 4255 5156 5418 820 Size: 197 926 1225 1087 901 262 1553 Fragments arranged by size: 1553 1225 1087 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3942 493 Size: 3449 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6151 SphI G_CATG'C Cuts at: 1132 2681 3509 1132 Size: 1549 828 3774 Fragments arranged by size: 3774 1549 828 SspI AAT'ATT Cuts at: 5 52 4075 5 Size: 47 4023 2081 Fragments arranged by size: 4023 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6151 StyI C'CwwG_G Cuts at: 513 1065 2004 3590 513 Size: 552 939 1586 3074 Fragments arranged by size: 3074 1586 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4178 4195 4348 4533 4978 325 Size: 1255 2598 17 153 185 445 1498 Fragments arranged by size: 2598 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2514 2553 2917 Size: 274 80 33 51 1443 537 39 364 Cuts at: 2917 4397 6109 96 Size: 1480 1712 138 Fragments arranged by size: 1712 1480 1443 537 364 274 138 80 51 39 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2396 2428 6111 6143 2396 Size: 32 3683 32 2404 Fragments arranged by size: 3683 2404 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6151 XbaI T'CTAG_A Cuts at: 3487 3487 Size: 6151 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6151 XmaI C'CCGG_G Cuts at: 1870 3476 1870 Size: 1606 4545 XmnI GAAnn'nnTTC Cuts at: 2883 3087 4280 2883 Size: 204 1193 4754 Fragments arranged by size: 4754 1193 204 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3959 276 Size: 53 83 186 936 2425 2468 Fragments arranged by size: 2468 2425 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI