(Circular) (Six-base) MAPSORT of: pFN21AB7721.seq Check: 5624 from: 1 to: 5989 pFN21AB7721 5989 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3799 278 Size: 53 83 186 936 2263 2468 Fragments arranged by size: 2468 2263 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3332 1937 Size: 1395 4594 Acc65I G'GTAC_C Cuts at: 3310 3310 Size: 5989 AclI AA'CG_TT Cuts at: 1563 4116 4489 1563 Size: 2553 373 3063 Fragments arranged by size: 3063 2553 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3122 4052 5436 5704 1632 Size: 1490 930 1384 268 1917 Fragments arranged by size: 1917 1490 1384 930 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5792 AflIII A'CryG_T Cuts at: 1203 2175 2619 4888 5865 1203 Size: 972 444 2269 977 1327 Fragments arranged by size: 2269 1327 977 972 444 AgeI A'CCGG_T Cuts at: 4820 4820 Size: 5989 AhdI GACnn_n'nnGTC Cuts at: 4718 4718 Size: 5989 AleI CACnn'nnGTG Cuts at: 1166 2523 1166 Size: 1357 4632 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5989 AlwNI CAG_nnn'CTG Cuts at: 3067 3732 5304 3067 Size: 665 1572 3752 Fragments arranged by size: 3752 1572 665 ApaLI G'TGCA_C Cuts at: 4046 5202 4046 Size: 1156 4833 ApoI r'AATT_y Cuts at: 1493 3298 3524 3676 5785 1493 Size: 1805 226 152 2109 1697 Fragments arranged by size: 2109 1805 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4543 160 Size: 872 3511 1606 Fragments arranged by size: 3511 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5989 AvaI C'yCGr_G Cuts at: 1870 1943 2297 2490 3314 1870 Size: 73 354 193 824 4545 Fragments arranged by size: 4545 824 354 193 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3047 3080 3047 Size: 33 5956 BamHI G'GATC_C Cuts at: 3319 3686 3319 Size: 367 5622 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3229 3310 4765 618 Size: 325 217 627 1442 81 1455 1842 Fragments arranged by size: 1842 1455 1442 627 325 217 81 BanII G_rGCy'C Cuts at: 1391 2304 3308 1391 Size: 913 1004 4072 Fragments arranged by size: 4072 1004 913 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2137 928 Size: 971 238 4780 Fragments arranged by size: 4780 971 238 BbvCI CC'TCA_GC Cuts at: 2816 2816 Size: 5989 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2787 2821 2981 3015 3022 3056 4180 Size: 34 850 34 160 34 7 34 1124 Cuts at: 4180 4214 1903 Size: 34 3678 Fragments arranged by size: 3678 1124 850 160 34 34 34 34 34 7 BciVI GTATCCnnnnn_n' Cuts at: 3881 5091 3881 Size: 1210 4779 BclI T'GATC_A Cuts at: 1548 1548 Size: 5989 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4600 136 Size: 107 122 71 631 605 2928 1525 Fragments arranged by size: 2928 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2367 5984 2367 Size: 3617 2372 BlpI GC'TnA_GC Cuts at: 3406 3406 Size: 5989 Bme1580I G_kGCm'C Cuts at: 1790 2670 4050 5206 1790 Size: 880 1380 1156 2573 Fragments arranged by size: 2573 1380 1156 880 BmgBI CAC'GTC Cuts at: 1318 2041 3142 1318 Size: 723 1101 4165 Fragments arranged by size: 4165 1101 723 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2282 2398 3720 Size: 416 53 83 399 24 865 116 1322 Cuts at: 3720 4678 442 Size: 958 1753 Fragments arranged by size: 1753 1322 958 865 416 399 116 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5989 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2332 2332 Size: 5989 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2489 2750 4649 1239 Size: 1250 261 1899 2579 Fragments arranged by size: 2579 1899 1250 261 Bpu10I CC'TnA_GC Cuts at: 2816 2816 Size: 5989 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2462 4114 4979 5277 2462 Size: 1652 865 298 3174 Fragments arranged by size: 3174 1652 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2129 3246 4652 882 Size: 617 630 1117 1406 2219 Fragments arranged by size: 2219 1406 1117 630 617 BsaAI yAC'GTr Cuts at: 493 1204 2754 3780 493 Size: 711 1550 1026 2702 Fragments arranged by size: 2702 1550 1026 711 BsaBI GATnn'nnATC Cuts at: 2462 5983 2462 Size: 3521 2468 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3796 4178 4813 275 Size: 53 83 186 936 2263 382 635 1451 Fragments arranged by size: 2263 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4422 4820 5094 5241 5652 4422 Size: 398 274 147 411 4759 Fragments arranged by size: 4759 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2147 2177 1178 Size: 30 165 30 744 30 4990 Fragments arranged by size: 4990 744 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2981 3206 3221 1186 Size: 1795 225 15 3954 Fragments arranged by size: 3954 1795 225 15 BseYI C'CCAG_C Cuts at: 2352 2881 5192 5688 2352 Size: 529 2311 496 2653 Fragments arranged by size: 2653 2311 529 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2387 1163 Size: 573 651 4765 Fragments arranged by size: 4765 651 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3439 4200 5228 1540 Size: 275 186 1438 761 1028 2301 Fragments arranged by size: 2301 1438 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2499 2766 4050 4135 5206 2499 Size: 267 1284 85 1071 3282 Fragments arranged by size: 3282 1284 1071 267 85 BsmI GAATG_Cn' Cuts at: 2843 3500 3593 2843 Size: 657 93 5239 Fragments arranged by size: 5239 657 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2304 2499 2670 2766 3308 4050 4135 Size: 399 514 195 171 96 542 742 85 Cuts at: 4135 5206 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 542 514 399 195 171 96 85 BspEI T'CCGG_A Cuts at: 5652 5652 Size: 5989 BspHI T'CATG_A Cuts at: 2565 3136 3878 5608 2565 Size: 571 742 1730 2946 Fragments arranged by size: 2946 1730 742 571 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3344 844 Size: 2500 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3876 1394 Size: 429 2053 3507 Fragments arranged by size: 3507 2053 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4484 4658 65 Size: 1183 3236 174 1396 Fragments arranged by size: 3236 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2716 4633 4820 1700 Size: 110 906 1917 187 2869 Fragments arranged by size: 2869 1917 906 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5989 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5989 BssSI C'ACGA_G Cuts at: 2876 4049 5061 2876 Size: 1173 1012 3804 Fragments arranged by size: 3804 1173 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5989 BstBI TT'CG_AA Cuts at: 4793 4793 Size: 5989 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5989 BstXI CCAn_nnnn'nTGG Cuts at: 2017 2017 Size: 5989 BstYI r'GATC_y Cuts at: 1972 2367 3075 3319 3686 4071 4088 5540 5626 Size: 395 708 244 367 385 17 1452 86 Cuts at: 5626 5638 5984 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 708 395 385 367 346 244 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3204 3204 Size: 5989 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 2521 513 Size: 552 939 12 505 3981 Fragments arranged by size: 3981 939 552 505 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2069 2292 2922 3291 487 Size: 683 56 843 223 630 369 3185 Fragments arranged by size: 3185 843 683 630 369 223 56 BtsI GCAGTG_nn' Cuts at: 3505 4299 4319 3505 Size: 794 20 5175 Fragments arranged by size: 5175 794 20 ClaI AT'CG_AT Cuts at: 1554 2797 1554 Size: 1243 4746 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2740 2775 529 Size: 35 2176 35 3743 Fragments arranged by size: 3743 2176 35 35 DraI TTT'AAA Cuts at: 3293 3293 Size: 5989 DraIII CAC_nnn'GTG Cuts at: 3125 3125 Size: 5989 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4996 809 Size: 507 3680 1802 Fragments arranged by size: 3680 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2644 3436 Size: 54 1425 50 275 18 177 637 792 Cuts at: 3436 4325 5954 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 792 637 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3436 1537 Size: 275 1624 4090 Fragments arranged by size: 4090 1624 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2946 3919 1353 Size: 1593 973 3423 Fragments arranged by size: 3423 1593 973 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3068 4576 4950 5096 387 Size: 1064 138 1479 1508 374 146 1280 Fragments arranged by size: 1508 1479 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2489 2750 3122 4052 4649 5436 5704 Size: 393 857 261 372 930 597 787 268 Cuts at: 5704 1239 Size: 1524 Fragments arranged by size: 1524 930 857 787 597 393 372 268 261 EcoNI CCTnn'n_nnAGG Cuts at: 2610 3166 2610 Size: 556 5433 EcoO109I rG'GnC_Cy Cuts at: 3200 3200 Size: 5989 EcoRI G'AATT_C Cuts at: 3298 3676 3298 Size: 378 5611 FspI TGC'GCA Cuts at: 4495 4495 Size: 5989 HaeII r_GCGC'y Cuts at: 1678 5136 1678 Size: 3458 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4630 4662 4704 4736 1373 Size: 32 135 32 3058 32 42 32 2626 Fragments arranged by size: 3058 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2446 3333 3579 669 Size: 988 281 508 887 246 3079 Fragments arranged by size: 3079 988 887 508 281 246 HindIII A'AGCT_T Cuts at: 748 2541 748 Size: 1793 4196 HpaI GTT'AAC Cuts at: 3579 3579 Size: 5989 KpnI G_GTAC'C Cuts at: 3314 3314 Size: 5989 MfeI C'AATT_G Cuts at: 3588 3588 Size: 5989 MluI A'CGCG_T Cuts at: 5865 5865 Size: 5989 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5103 5287 1305 Size: 3798 184 2007 Fragments arranged by size: 3798 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 3990 Fragments arranged by size: 3990 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2234 2523 2570 3024 3261 3947 4306 Size: 648 1068 289 47 454 237 686 359 Cuts at: 4306 4465 518 Size: 159 2042 Fragments arranged by size: 2042 1068 686 648 454 359 289 237 159 47 MspA1I CmG'CkG Cuts at: 1606 2356 2919 3405 4082 5230 5475 1606 Size: 750 563 486 677 1148 245 2120 Fragments arranged by size: 2120 1148 750 677 563 486 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2521 513 Size: 552 939 517 3981 Fragments arranged by size: 3981 939 552 517 NdeI CA'TA_TG Cuts at: 387 2929 387 Size: 2542 3447 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5989 NotI GC'GGCC_GC Cuts at: 3436 3436 Size: 5989 NspI r_CATG'y Cuts at: 1132 1229 2179 2623 3347 4892 1132 Size: 97 950 444 724 1545 2229 Fragments arranged by size: 2229 1545 950 724 444 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5989 PciI A'CATG_T Cuts at: 2175 2619 4888 2175 Size: 444 2269 3276 Fragments arranged by size: 3276 2269 444 PflMI CCAn_nnn'nTGG Cuts at: 1108 2089 2107 2388 2734 3067 1108 Size: 981 18 281 346 333 4030 Fragments arranged by size: 4030 981 346 333 281 18 PfoI T'CCnGG_A Cuts at: 2466 2466 Size: 5989 PmeI GTTT'AAAC Cuts at: 3293 3293 Size: 5989 PmlI CAC'GTG Cuts at: 2754 2754 Size: 5989 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4058 4090 5571 5603 4058 Size: 32 1481 32 4444 Fragments arranged by size: 4444 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3200 3200 Size: 5989 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5989 PsiI TTA'TAA Cuts at: 3559 3559 Size: 5989 PstI C_TGCA'G Cuts at: 830 2217 3151 3341 830 Size: 1387 934 190 3478 Fragments arranged by size: 3478 1387 934 190 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5989 PvuII CAG'CTG Cuts at: 2356 2356 Size: 5989 SalI G'TCGA_C Cuts at: 1936 3331 1936 Size: 1395 4594 SbfI CC_TGCA'GG Cuts at: 3341 3341 Size: 5989 ScaI AGT'ACT Cuts at: 2996 4237 5949 2996 Size: 1241 1712 3036 Fragments arranged by size: 3036 1712 1241 SexAI A'CCwGG_T Cuts at: 2727 2727 Size: 5989 SfcI C'TryA_G Cuts at: 826 1045 2213 2977 3147 3214 3337 4472 5153 Size: 219 1168 764 170 67 123 1135 681 Cuts at: 5153 5344 826 Size: 191 1471 Fragments arranged by size: 1471 1168 1135 764 681 219 191 170 123 67 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5989 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5989 SmaI CCC'GGG Cuts at: 1872 2299 3316 1872 Size: 427 1017 4545 Fragments arranged by size: 4545 1017 427 SmlI C'TyrA_G Cuts at: 820 1017 1943 2477 4093 4994 5256 820 Size: 197 926 534 1616 901 262 1553 Fragments arranged by size: 1616 1553 926 901 534 262 197 SnaBI TAC'GTA Cuts at: 493 3780 493 Size: 3287 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5989 SphI G_CATG'C Cuts at: 1132 3347 1132 Size: 2215 3774 SspI AAT'ATT Cuts at: 5 52 3913 5 Size: 47 3861 2081 Fragments arranged by size: 3861 2081 47 StuI AGG'CCT Cuts at: 1517 2162 2426 1517 Size: 645 264 5080 Fragments arranged by size: 5080 645 264 StyI C'CwwG_G Cuts at: 513 1065 2004 2521 3428 513 Size: 552 939 517 907 3074 Fragments arranged by size: 3074 939 907 552 517 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2873 3247 4016 4033 4186 4371 4816 Size: 1255 1293 374 769 17 153 185 445 Cuts at: 4816 325 Size: 1498 Fragments arranged by size: 1498 1293 1255 769 445 374 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2994 4235 5947 Size: 274 80 33 51 1443 1017 1241 1712 Cuts at: 5947 96 Size: 138 Fragments arranged by size: 1712 1443 1241 1017 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3004 3036 3111 3143 5949 5981 3004 Size: 32 75 32 2806 32 3012 Fragments arranged by size: 3012 2806 75 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2561 2572 1316 Size: 1245 11 4733 Fragments arranged by size: 4733 1245 11 XbaI T'CTAG_A Cuts at: 3325 3325 Size: 5989 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2060 2060 Size: 5989 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5989 XmaI C'CCGG_G Cuts at: 1870 2297 3314 1870 Size: 427 1017 4545 Fragments arranged by size: 4545 1017 427 XmnI GAAnn'nnTTC Cuts at: 4118 4118 Size: 5989 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3797 276 Size: 53 83 186 936 2263 2468 Fragments arranged by size: 2468 2263 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI