Restriction map for
pFN21AB7721
(Linear) (Six-base) MAPSORT of: KIBB7721.seq Check: 1697 from: 1 to: 1284
KIBB7721.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1117 1284
Size: 1117 167
AflIII A'CryG_T
Cuts at: 0 170 614 1284
Size: 170 444 670
Fragments arranged by size:
670 444 170
AleI CACnn'nnGTG
Cuts at: 0 518 1284
Size: 518 766
AlwNI CAG_nnn'CTG
Cuts at: 0 1062 1284
Size: 1062 222
AvaI C'yCGr_G
Cuts at: 0 292 485 1284
Size: 292 193 799
Fragments arranged by size:
799 292 193
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1042 1075 1284
Size: 1042 33 209
Fragments arranged by size:
1042 209 33
BanI G'GyrC_C
Cuts at: 0 1224 1284
Size: 1224 60
BanII G_rGCy'C
Cuts at: 0 299 1284
Size: 299 985
BbsI GAAGACnn'nnnn_
Cuts at: 0 132 1284
Size: 132 1152
BbvCI CC'TCA_GC
Cuts at: 0 811 1284
Size: 811 473
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 782 816 976 1010 1017 1051 1284
Size: 782 34 160 34 7 34 233
Fragments arranged by size:
782 233 160 34 34 34 7
BglII A'GATC_T
Cuts at: 0 362 1284
Size: 362 922
Bme1580I G_kGCm'C
Cuts at: 0 665 1284
Size: 665 619
BmgBI CAC'GTC
Cuts at: 0 36 1137 1284
Size: 36 1101 147
Fragments arranged by size:
1101 147 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 277 393 1284
Size: 277 116 891
Fragments arranged by size:
891 277 116
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 327 1284
Size: 327 957
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 745 1284
Size: 484 261 539
Fragments arranged by size:
539 484 261
Bpu10I CC'TnA_GC
Cuts at: 0 811 1284
Size: 811 473
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 1284
Size: 457 827
BsaI GGTCTCn'nnnn_
Cuts at: 0 124 1241 1284
Size: 124 1117 43
Fragments arranged by size:
1117 124 43
BsaAI yAC'GTr
Cuts at: 0 749 1284
Size: 749 535
BsaBI GATnn'nnATC
Cuts at: 0 457 1284
Size: 457 827
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 142 172 1284
Size: 142 30 1112
Fragments arranged by size:
1112 142 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 976 1201 1216 1284
Size: 976 225 15 68
Fragments arranged by size:
976 225 68 15
BseYI C'CCAG_C
Cuts at: 0 347 876 1284
Size: 347 529 408
Fragments arranged by size:
529 408 347
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 1284
Size: 382 902
BsiHKAI G_wGCw'C
Cuts at: 0 494 761 1284
Size: 494 267 523
Fragments arranged by size:
523 494 267
BsmI GAATG_Cn'
Cuts at: 0 838 1284
Size: 838 446
Bsp1286I G_dGCh'C
Cuts at: 0 299 494 665 761 1284
Size: 299 195 171 96 523
Fragments arranged by size:
523 299 195 171 96
BspHI T'CATG_A
Cuts at: 0 560 1131 1284
Size: 560 571 153
Fragments arranged by size:
571 560 153
BsrFI r'CCGG_y
Cuts at: 0 711 1284
Size: 711 573
BssSI C'ACGA_G
Cuts at: 0 871 1284
Size: 871 413
BstXI CCAn_nnnn'nTGG
Cuts at: 0 12 1284
Size: 12 1272
BstYI r'GATC_y
Cuts at: 0 362 1070 1284
Size: 362 708 214
Fragments arranged by size:
708 362 214
Bsu36I CC'TnA_GG
Cuts at: 0 1199 1284
Size: 1199 85
BtgI C'CryG_G
Cuts at: 0 11 516 1284
Size: 11 505 768
Fragments arranged by size:
768 505 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 64 287 917 1284
Size: 64 223 630 367
Fragments arranged by size:
630 367 223 64
ClaI AT'CG_AT
Cuts at: 0 792 1284
Size: 792 492
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 735 770 1284
Size: 735 35 514
Fragments arranged by size:
735 514 35
DraIII CAC_nnn'GTG
Cuts at: 0 1120 1284
Size: 1120 164
EaeI y'GGCC_r
Cuts at: 0 2 639 1284
Size: 2 637 645
Fragments arranged by size:
645 637 2
EarI CTCTTCn'nnn_
Cuts at: 0 941 1284
Size: 941 343
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1063 1284
Size: 1063 221
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 745 1117 1284
Size: 484 261 372 167
Fragments arranged by size:
484 372 261 167
EcoNI CCTnn'n_nnAGG
Cuts at: 0 605 1161 1284
Size: 605 556 123
Fragments arranged by size:
605 556 123
EcoO109I rG'GnC_Cy
Cuts at: 0 1195 1284
Size: 1195 89
HincII GTy'rAC
Cuts at: 0 441 1284
Size: 441 843
HindIII A'AGCT_T
Cuts at: 0 536 1284
Size: 536 748
MscI TGG'CCA
Cuts at: 0 4 1284
Size: 4 1280
MslI CAynn'nnrTG
Cuts at: 0 229 518 565 1019 1256 1284
Size: 229 289 47 454 237 28
Fragments arranged by size:
454 289 237 229 47 28
MspA1I CmG'CkG
Cuts at: 0 351 914 1284
Size: 351 563 370
Fragments arranged by size:
563 370 351
NcoI C'CATG_G
Cuts at: 0 516 1284
Size: 516 768
NdeI CA'TA_TG
Cuts at: 0 924 1284
Size: 924 360
NspI r_CATG'y
Cuts at: 0 174 618 1284
Size: 174 444 666
Fragments arranged by size:
666 444 174
PciI A'CATG_T
Cuts at: 0 170 614 1284
Size: 170 444 670
Fragments arranged by size:
670 444 170
PflMI CCAn_nnn'nTGG
Cuts at: 0 84 102 383 729 1062 1284
Size: 84 18 281 346 333 222
Fragments arranged by size:
346 333 281 222 84 18
PfoI T'CCnGG_A
Cuts at: 0 461 1284
Size: 461 823
PmlI CAC'GTG
Cuts at: 0 749 1284
Size: 749 535
PpuMI rG'GwC_Cy
Cuts at: 0 1195 1284
Size: 1195 89
PstI C_TGCA'G
Cuts at: 0 212 1146 1284
Size: 212 934 138
Fragments arranged by size:
934 212 138
PvuII CAG'CTG
Cuts at: 0 351 1284
Size: 351 933
ScaI AGT'ACT
Cuts at: 0 991 1284
Size: 991 293
SexAI A'CCwGG_T
Cuts at: 0 722 1284
Size: 722 562
SfcI C'TryA_G
Cuts at: 0 208 972 1142 1209 1284
Size: 208 764 170 67 75
Fragments arranged by size:
764 208 170 75 67
SmaI CCC'GGG
Cuts at: 0 294 1284
Size: 294 990
SmlI C'TyrA_G
Cuts at: 0 472 1284
Size: 472 812
StuI AGG'CCT
Cuts at: 0 157 421 1284
Size: 157 264 863
Fragments arranged by size:
863 264 157
StyI C'CwwG_G
Cuts at: 0 516 1284
Size: 516 768
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 868 1242 1284
Size: 868 374 42
Fragments arranged by size:
868 374 42
TatI w'GTAC_w
Cuts at: 0 989 1284
Size: 989 295
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 999 1031 1106 1138 1284
Size: 999 32 75 32 146
Fragments arranged by size:
999 146 75 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 556 567 1284
Size: 556 11 717
Fragments arranged by size:
717 556 11
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 55 1284
Size: 55 1229
XmaI C'CCGG_G
Cuts at: 0 292 1284
Size: 292 992
Enzymes that do cut:
AcuI AflIII AleI AlwNI AvaI BaeI BanI BanII
BbsI BbvCI BcgI BglII Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrFI BssSI
BstXI BstYI Bsu36I BtgI BtgZI ClaI CspCI DraIII
EaeI EarI EciI Eco57MI EcoNI EcoO109I HincII HindIII
MscI MslI MspA1I NcoI NdeI NspI PciI PflMI
PfoI PmlI PpuMI PstI PvuII ScaI SexAI SfcI
SmaI SmlI StuI StyI TaqII TatI TstI Tth111I
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BamHI BbeI BciVI BclI BfrBI BglI
BlpI BmtI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI
BspMI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstEII
BstZ17I BtsI DraI DrdI EagI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PasI PmeI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI
XmnI ZraI