(Circular) (Six-base) MAPSORT of: pFN21AB7654.seq Check: 1061 from: 1 to: 5818 pFN21AB7654 5818 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2699 2699 Size: 5818 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3628 278 Size: 53 83 186 936 2092 2468 Fragments arranged by size: 2468 2092 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3161 1937 Size: 1224 4594 Acc65I G'GTAC_C Cuts at: 2134 3139 2134 Size: 1005 4813 AclI AA'CG_TT Cuts at: 1563 3945 4318 1563 Size: 2382 373 3063 Fragments arranged by size: 3063 2382 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2564 3881 5265 5533 1632 Size: 932 1317 1384 268 1917 Fragments arranged by size: 1917 1384 1317 932 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5621 AflIII A'CryG_T Cuts at: 1203 2461 4717 5694 1203 Size: 1258 2256 977 1327 Fragments arranged by size: 2256 1327 1258 977 AgeI A'CCGG_T Cuts at: 4649 4649 Size: 5818 AhdI GACnn_n'nnGTC Cuts at: 4547 4547 Size: 5818 AleI CACnn'nnGTG Cuts at: 1166 2881 1166 Size: 1715 4103 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2370 1184 Size: 1186 4632 AlwNI CAG_nnn'CTG Cuts at: 2599 2691 3561 5133 2599 Size: 92 870 1572 3284 Fragments arranged by size: 3284 1572 870 92 ApaI G_GGCC'C Cuts at: 2104 2104 Size: 5818 ApaLI G'TGCA_C Cuts at: 3875 5031 3875 Size: 1156 4662 ApoI r'AATT_y Cuts at: 1493 2451 2611 3127 3353 3505 5614 1493 Size: 958 160 516 226 152 2109 1697 Fragments arranged by size: 2109 1697 958 516 226 160 152 AseI AT'TA_AT Cuts at: 160 1032 4372 160 Size: 872 3340 1606 Fragments arranged by size: 3340 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5818 AvaI C'yCGr_G Cuts at: 1870 1943 3143 1870 Size: 73 1200 4545 Fragments arranged by size: 4545 1200 73 BamHI G'GATC_C Cuts at: 3148 3515 3148 Size: 367 5451 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2080 2134 3139 4594 618 Size: 325 217 627 293 54 1005 1455 1842 Fragments arranged by size: 1842 1455 1005 627 325 293 217 54 BanII G_rGCy'C Cuts at: 1391 2104 3137 1391 Size: 713 1033 4072 Fragments arranged by size: 4072 1033 713 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4847 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4009 4043 1903 Size: 34 2072 34 3678 Fragments arranged by size: 3678 2072 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3710 4920 3710 Size: 1210 4608 BclI T'GATC_A Cuts at: 1548 1548 Size: 5818 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2099 4429 136 Size: 107 122 71 631 605 427 2330 1525 Fragments arranged by size: 2330 1525 631 605 427 122 107 71 BglII A'GATC_T Cuts at: 2489 5813 2489 Size: 3324 2494 BlpI GC'TnA_GC Cuts at: 2775 3235 2775 Size: 460 5358 Bme1580I G_kGCm'C Cuts at: 1790 2104 2809 3879 5035 1790 Size: 314 705 1070 1156 2573 Fragments arranged by size: 2573 1156 1070 705 314 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5818 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2122 2155 2808 Size: 416 53 83 399 24 705 33 653 Cuts at: 2808 2955 3549 4507 442 Size: 147 594 958 1753 Fragments arranged by size: 1753 958 705 653 594 416 399 147 83 53 33 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5818 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2621 2627 2855 2894 2999 4478 1239 Size: 1382 6 228 39 105 1479 2579 Fragments arranged by size: 2579 1479 1382 228 105 39 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2481 3943 4808 5106 2481 Size: 1462 865 298 3193 Fragments arranged by size: 3193 1462 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4481 882 Size: 617 2982 2219 Fragments arranged by size: 2982 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3609 493 Size: 711 2405 2702 Fragments arranged by size: 2702 2405 711 BsaBI GATnn'nnATC Cuts at: 5812 5812 Size: 5818 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3625 4007 4642 275 Size: 53 83 186 936 2092 382 635 1451 Fragments arranged by size: 2092 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4251 4649 4923 5070 5481 4251 Size: 398 274 147 411 4588 Fragments arranged by size: 4588 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2231 2261 1178 Size: 30 165 30 828 30 4735 Fragments arranged by size: 4735 828 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2200 2348 2393 2714 2737 1186 Size: 1014 148 45 321 23 4267 Fragments arranged by size: 4267 1014 321 148 45 23 BseYI C'CCAG_C Cuts at: 2185 5021 5517 2185 Size: 2836 496 2486 Fragments arranged by size: 2836 2486 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2985 1163 Size: 573 1249 3996 Fragments arranged by size: 3996 1249 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3268 4029 5057 1540 Size: 275 186 1267 761 1028 2301 Fragments arranged by size: 2301 1267 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2171 3879 3964 5035 2171 Size: 1708 85 1071 2954 Fragments arranged by size: 2954 1708 1071 85 BsmI GAATG_Cn' Cuts at: 3329 3422 3329 Size: 93 5725 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2104 2171 2809 3137 3879 3964 5035 Size: 399 314 67 638 328 742 85 1071 Cuts at: 5035 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 638 399 328 314 85 67 BspEI T'CCGG_A Cuts at: 5481 5481 Size: 5818 BspHI T'CATG_A Cuts at: 3707 5437 3707 Size: 1730 4088 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2046 2699 2743 3173 844 Size: 1202 653 44 430 3489 Fragments arranged by size: 3489 1202 653 430 44 BsrBI CCG'CTC Cuts at: 1394 1823 3705 1394 Size: 429 1882 3507 Fragments arranged by size: 3507 1882 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4313 4487 65 Size: 1183 3065 174 1396 Fragments arranged by size: 3065 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4462 4649 1700 Size: 110 2652 187 2869 Fragments arranged by size: 2869 2652 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5818 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5818 BssSI C'ACGA_G Cuts at: 3878 4890 3878 Size: 1012 4806 BstAPI GCAn_nnn'nTGC Cuts at: 794 2691 2947 794 Size: 1897 256 3665 Fragments arranged by size: 3665 1897 256 BstBI TT'CG_AA Cuts at: 2479 4622 2479 Size: 2143 3675 BstEII G'GTnAC_C Cuts at: 1184 2027 1184 Size: 843 4975 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2112 2918 2011 Size: 101 806 4911 Fragments arranged by size: 4911 806 101 BstYI r'GATC_y Cuts at: 1972 2275 2489 3148 3515 3900 3917 5369 5455 Size: 303 214 659 367 385 17 1452 86 Cuts at: 5455 5467 5813 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 659 385 367 346 303 214 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2952 2952 Size: 5818 BtgI C'CryG_G Cuts at: 513 1065 2004 2255 2649 513 Size: 552 939 251 394 3682 Fragments arranged by size: 3682 939 552 394 251 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5079 Fragments arranged by size: 5079 683 56 BtsI GCAGTG_nn' Cuts at: 3334 4128 4148 3334 Size: 794 20 5004 Fragments arranged by size: 5004 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5818 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5783 DraI TTT'AAA Cuts at: 3122 3122 Size: 5818 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4825 809 Size: 507 3509 1802 Fragments arranged by size: 3509 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2984 3265 4154 Size: 54 1425 50 275 18 1154 281 889 Cuts at: 4154 5783 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1154 889 281 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3265 1537 Size: 275 1453 4090 Fragments arranged by size: 4090 1453 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2378 3005 3748 1353 Size: 1025 627 743 3423 Fragments arranged by size: 3423 1025 743 627 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4405 4779 4925 387 Size: 1064 138 2816 374 146 1280 Fragments arranged by size: 2816 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2564 2621 2627 2855 2894 2999 3881 Size: 393 932 57 6 228 39 105 882 Cuts at: 3881 4478 5265 5533 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 932 882 787 597 393 268 228 105 57 39 6 EcoNI CCTnn'n_nnAGG Cuts at: 2054 2054 Size: 5818 EcoO109I rG'GnC_Cy Cuts at: 2100 2101 2307 2821 2914 3023 2100 Size: 1 206 514 93 109 4895 Fragments arranged by size: 4895 514 206 109 93 1 EcoRI G'AATT_C Cuts at: 3127 3505 3127 Size: 378 5440 EcoRV GAT'ATC Cuts at: 2664 2664 Size: 5818 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2612 2612 Size: 5818 FspI TGC'GCA Cuts at: 4324 4324 Size: 5818 HaeII r_GCGC'y Cuts at: 1678 2120 4965 1678 Size: 442 2845 2531 Fragments arranged by size: 2845 2531 442 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2413 2445 2987 3012 3019 Size: 32 135 32 841 32 542 25 7 Cuts at: 3019 3044 3095 3127 4459 4491 4533 4565 1373 Size: 25 51 32 1332 32 42 32 2626 Fragments arranged by size: 2626 1332 841 542 135 51 42 32 32 32 32 32 32 25 25 7 HincII GTy'rAC Cuts at: 669 1657 1938 3162 3408 669 Size: 988 281 1224 246 3079 Fragments arranged by size: 3079 1224 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5818 HpaI GTT'AAC Cuts at: 3408 3408 Size: 5818 KpnI G_GTAC'C Cuts at: 2138 3143 2138 Size: 1005 4813 MfeI C'AATT_G Cuts at: 3417 3417 Size: 5818 MluI A'CGCG_T Cuts at: 5694 5694 Size: 5818 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2238 2868 4932 5116 1305 Size: 933 630 2064 184 2007 Fragments arranged by size: 2064 2007 933 630 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2986 10 Size: 54 1425 343 1154 2842 Fragments arranged by size: 2842 1425 1154 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2881 3776 4135 4294 518 Size: 648 1715 895 359 159 2042 Fragments arranged by size: 2042 1715 895 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2774 2833 3234 3911 5059 5304 1606 Size: 1168 59 401 677 1148 245 2120 Fragments arranged by size: 2120 1168 1148 677 401 245 59 NcoI C'CATG_G Cuts at: 513 1065 2004 2649 513 Size: 552 939 645 3682 Fragments arranged by size: 3682 939 645 552 NdeI CA'TA_TG Cuts at: 387 2328 387 Size: 1941 3877 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5818 NotI GC'GGCC_GC Cuts at: 3265 3265 Size: 5818 NspI r_CATG'y Cuts at: 1132 1229 2950 3176 4721 1132 Size: 97 1721 226 1545 2229 Fragments arranged by size: 2229 1721 1545 226 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5818 PciI A'CATG_T Cuts at: 4717 4717 Size: 5818 PflMI CCAn_nnn'nTGG Cuts at: 1108 2572 2650 2887 1108 Size: 1464 78 237 4039 Fragments arranged by size: 4039 1464 237 78 PmeI GTTT'AAAC Cuts at: 3122 3122 Size: 5818 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3887 3919 5400 5432 3887 Size: 32 1481 32 4273 Fragments arranged by size: 4273 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2307 2821 3023 2307 Size: 514 202 5102 Fragments arranged by size: 5102 514 202 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5818 PsiI TTA'TAA Cuts at: 3388 3388 Size: 5818 PspOMI G'GGCC_C Cuts at: 2100 2100 Size: 5818 PstI C_TGCA'G Cuts at: 830 2853 3042 3170 830 Size: 2023 189 128 3478 Fragments arranged by size: 3478 2023 189 128 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5818 PvuII CAG'CTG Cuts at: 2774 2833 2774 Size: 59 5759 SalI G'TCGA_C Cuts at: 1936 3160 1936 Size: 1224 4594 SanDI GG'GwC_CC Cuts at: 2307 2307 Size: 5818 SbfI CC_TGCA'GG Cuts at: 3042 3170 3042 Size: 128 5690 ScaI AGT'ACT Cuts at: 2367 4066 5778 2367 Size: 1699 1712 2407 Fragments arranged by size: 2407 1712 1699 SfcI C'TryA_G Cuts at: 826 1045 2293 2849 3038 3166 4301 4982 5173 Size: 219 1248 556 189 128 1135 681 191 Cuts at: 5173 826 Size: 1471 Fragments arranged by size: 1471 1248 1135 681 556 219 191 189 128 SfiI GGCCn_nnn'nGGCC Cuts at: 2099 2099 Size: 5818 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5818 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5818 SmaI CCC'GGG Cuts at: 1872 3145 1872 Size: 1273 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2496 3922 4823 5085 820 Size: 197 926 553 1426 901 262 1553 Fragments arranged by size: 1553 1426 926 901 553 262 197 SnaBI TAC'GTA Cuts at: 493 3609 493 Size: 3116 2702 SpeI A'CTAG_T Cuts at: 152 2707 152 Size: 2555 3263 SphI G_CATG'C Cuts at: 1132 3176 1132 Size: 2044 3774 SspI AAT'ATT Cuts at: 5 52 3742 5 Size: 47 3690 2081 Fragments arranged by size: 3690 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5818 StyI C'CwwG_G Cuts at: 513 1065 2004 2649 3257 513 Size: 552 939 645 608 3074 Fragments arranged by size: 3074 939 645 608 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3845 3862 4015 4200 4645 325 Size: 1255 2265 17 153 185 445 1498 Fragments arranged by size: 2265 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2365 2590 2742 Size: 274 80 33 51 1443 388 225 152 Cuts at: 2742 2905 4064 5776 96 Size: 163 1159 1712 138 Fragments arranged by size: 1712 1443 1159 388 274 225 163 152 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2231 2263 5778 5810 2231 Size: 32 3515 32 2239 Fragments arranged by size: 3515 2239 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5818 XbaI T'CTAG_A Cuts at: 2278 2623 3154 2278 Size: 345 531 4942 Fragments arranged by size: 4942 531 345 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5818 XmaI C'CCGG_G Cuts at: 1870 3143 1870 Size: 1273 4545 XmnI GAAnn'nnTTC Cuts at: 2451 3947 2451 Size: 1496 4322 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3626 276 Size: 53 83 186 936 2092 2468 Fragments arranged by size: 2468 2092 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfoI SrfI SwaI XcmI