(Circular) (Six-base) MAPSORT of: pFN21AB7631.seq Check: 5881 from: 1 to: 5665 pFN21AB7631 5665 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3475 278 Size: 53 83 186 936 1939 2468 Fragments arranged by size: 2468 1939 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2037 3008 1937 Size: 100 971 4594 Fragments arranged by size: 4594 971 100 Acc65I G'GTAC_C Cuts at: 2986 2986 Size: 5665 AclI AA'CG_TT Cuts at: 1563 3792 4165 1563 Size: 2229 373 3063 Fragments arranged by size: 3063 2229 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3728 5112 5380 1632 Size: 2096 1384 268 1917 Fragments arranged by size: 2096 1917 1384 268 AfeI AGC'GCT Cuts at: 2405 2405 Size: 5665 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5468 AflIII A'CryG_T Cuts at: 1203 2598 4564 5541 1203 Size: 1395 1966 977 1327 Fragments arranged by size: 1966 1395 1327 977 AgeI A'CCGG_T Cuts at: 4496 4496 Size: 5665 AhdI GACnn_n'nnGTC Cuts at: 2758 4394 2758 Size: 1636 4029 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5665 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2874 2943 1184 Size: 1690 69 3906 Fragments arranged by size: 3906 1690 69 AlwNI CAG_nnn'CTG Cuts at: 2491 3408 4980 2491 Size: 917 1572 3176 Fragments arranged by size: 3176 1572 917 ApaI G_GGCC'C Cuts at: 2552 2552 Size: 5665 ApaLI G'TGCA_C Cuts at: 2210 3722 4878 2210 Size: 1512 1156 2997 Fragments arranged by size: 2997 1512 1156 ApoI r'AATT_y Cuts at: 1493 2974 3200 3352 5461 1493 Size: 1481 226 152 2109 1697 Fragments arranged by size: 2109 1697 1481 226 152 AseI AT'TA_AT Cuts at: 160 1032 4219 160 Size: 872 3187 1606 Fragments arranged by size: 3187 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5665 AvaI C'yCGr_G Cuts at: 1870 1943 2221 2560 2653 2723 2990 1870 Size: 73 278 339 93 70 267 4545 Fragments arranged by size: 4545 339 278 267 93 73 70 BamHI G'GATC_C Cuts at: 2995 3362 2995 Size: 367 5298 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2262 2361 2522 2660 2687 Size: 325 217 627 475 99 161 138 27 Cuts at: 2687 2704 2800 2986 4441 618 Size: 17 96 186 1455 1842 Fragments arranged by size: 1842 1455 627 475 325 217 186 161 138 99 96 27 17 BanII G_rGCy'C Cuts at: 1391 2552 2795 2984 1391 Size: 1161 243 189 4072 Fragments arranged by size: 4072 1161 243 189 BbeI G_GCGC'C Cuts at: 2365 2526 2664 2691 2708 2804 2365 Size: 161 138 27 17 96 5226 Fragments arranged by size: 5226 161 138 96 27 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2142 928 Size: 971 243 4451 Fragments arranged by size: 4451 971 243 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2777 2811 3856 3890 1903 Size: 34 840 34 1045 34 3678 Fragments arranged by size: 3678 1045 840 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3557 4767 3557 Size: 1210 4455 BclI T'GATC_A Cuts at: 1548 1548 Size: 5665 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2260 2300 2571 Size: 107 122 71 631 605 588 40 271 Cuts at: 2571 4276 136 Size: 1705 1525 Fragments arranged by size: 1705 1525 631 605 588 271 122 107 71 40 BglII A'GATC_T Cuts at: 5660 5660 Size: 5665 BlpI GC'TnA_GC Cuts at: 3082 3082 Size: 5665 Bme1580I G_kGCm'C Cuts at: 1790 2214 2265 2552 2927 3726 4882 1790 Size: 424 51 287 375 799 1156 2573 Fragments arranged by size: 2573 1156 799 424 375 287 51 BmgBI CAC'GTC Cuts at: 1318 2412 2824 1318 Size: 1094 412 4159 Fragments arranged by size: 4159 1094 412 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2893 3396 4354 Size: 416 53 83 399 24 1476 503 958 Cuts at: 4354 442 Size: 1753 Fragments arranged by size: 1753 1476 958 503 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5665 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2797 2797 Size: 5665 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4325 1239 Size: 3086 2579 Bpu10I CC'TnA_GC Cuts at: 2369 2369 Size: 5665 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2384 3790 4655 4953 2384 Size: 1406 865 298 3096 Fragments arranged by size: 3096 1406 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4328 882 Size: 617 2829 2219 Fragments arranged by size: 2829 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2161 2231 3456 493 Size: 711 957 70 1225 2702 Fragments arranged by size: 2702 1225 957 711 70 BsaBI GATnn'nnATC Cuts at: 5659 5659 Size: 5665 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2362 2508 2523 2661 Size: 53 83 186 936 829 146 15 138 Cuts at: 2661 2688 2705 2801 2910 3472 3854 4489 275 Size: 27 17 96 109 562 382 635 1451 Fragments arranged by size: 1451 936 829 635 562 382 186 146 138 109 96 83 53 27 17 15 BsaWI w'CCGG_w Cuts at: 2905 4098 4496 4770 4917 5328 2905 Size: 1193 398 274 147 411 3242 Fragments arranged by size: 3242 1193 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2000 2030 1178 Size: 30 165 30 597 30 4813 Fragments arranged by size: 4813 597 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5665 BseYI C'CCAG_C Cuts at: 2255 2708 2867 4868 5364 2255 Size: 453 159 2001 496 2556 Fragments arranged by size: 2556 2001 496 453 159 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2392 1163 Size: 573 656 4436 Fragments arranged by size: 4436 656 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2332 3115 3876 4904 1540 Size: 275 186 331 783 761 1028 2301 Fragments arranged by size: 2301 1028 783 761 331 275 186 BsiHKAI G_wGCw'C Cuts at: 2190 2214 2580 2964 3726 3811 4882 2190 Size: 24 366 384 762 85 1071 2973 Fragments arranged by size: 2973 1071 762 384 366 85 24 BsmI GAATG_Cn' Cuts at: 3176 3269 3176 Size: 93 5572 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2190 2214 2265 2552 2580 2795 2927 Size: 399 400 24 51 287 28 215 132 Cuts at: 2927 2964 2984 3726 3811 4882 1391 Size: 37 20 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 400 399 287 215 132 85 51 37 28 24 20 BspEI T'CCGG_A Cuts at: 5328 5328 Size: 5665 BspHI T'CATG_A Cuts at: 3554 5284 3554 Size: 1730 3935 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2130 3020 844 Size: 1286 890 3489 Fragments arranged by size: 3489 1286 890 BsrBI CCG'CTC Cuts at: 1394 1823 2026 2359 2658 3552 1394 Size: 429 203 333 299 894 3507 Fragments arranged by size: 3507 894 429 333 299 203 BsrDI GCAATG_nn' Cuts at: 65 1248 4160 4334 65 Size: 1183 2912 174 1396 Fragments arranged by size: 2912 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4309 4496 1700 Size: 110 2499 187 2869 Fragments arranged by size: 2869 2499 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5665 BssHII G'CGCG_C Cuts at: 1925 2166 2248 2250 1925 Size: 241 82 2 5340 Fragments arranged by size: 5340 241 82 2 BssSI C'ACGA_G Cuts at: 3725 4737 3725 Size: 1012 4653 BstAPI GCAn_nnn'nTGC Cuts at: 794 2304 2491 794 Size: 1510 187 3968 Fragments arranged by size: 3968 1510 187 BstBI TT'CG_AA Cuts at: 2133 4469 2133 Size: 2336 3329 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5665 BstXI CCAn_nnnn'nTGG Cuts at: 2770 2770 Size: 5665 BstYI r'GATC_y Cuts at: 1972 2448 2995 3362 3747 3764 5216 5302 5314 Size: 476 547 367 385 17 1452 86 12 Cuts at: 5314 5660 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 547 476 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2202 2566 513 Size: 552 939 198 364 3612 Fragments arranged by size: 3612 939 552 364 198 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4926 Fragments arranged by size: 4926 683 56 BtsI GCAGTG_nn' Cuts at: 2927 3181 3975 3995 2927 Size: 254 794 20 4597 Fragments arranged by size: 4597 794 254 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5665 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5630 DraI TTT'AAA Cuts at: 2969 2969 Size: 5665 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4672 809 Size: 507 3356 1802 Fragments arranged by size: 3356 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3112 4001 5630 Size: 54 1425 50 275 18 1282 889 1629 Cuts at: 5630 8 Size: 43 Fragments arranged by size: 1629 1425 1282 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3112 1537 Size: 275 1300 4090 Fragments arranged by size: 4090 1300 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2178 2723 3595 1353 Size: 825 545 872 3423 Fragments arranged by size: 3423 872 825 545 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2686 2834 4252 4626 4772 387 Size: 1064 138 1097 148 1418 374 146 1280 Fragments arranged by size: 1418 1280 1097 1064 374 148 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3728 4325 5112 5380 1239 Size: 393 2096 597 787 268 1524 Fragments arranged by size: 2096 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2479 2479 Size: 5665 EcoRI G'AATT_C Cuts at: 2974 3352 2974 Size: 378 5287 FspI TGC'GCA Cuts at: 2456 4171 2456 Size: 1715 3950 HaeII r_GCGC'y Cuts at: 1678 2365 2407 2526 2664 2691 2708 2804 4812 Size: 687 42 119 138 27 17 96 2008 Cuts at: 4812 1678 Size: 2531 Fragments arranged by size: 2531 2008 687 138 119 96 42 27 17 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2000 2032 2744 2776 4306 Size: 32 135 32 428 32 712 32 1530 Cuts at: 4306 4338 4380 4412 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1530 712 428 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3009 3255 669 Size: 988 281 1071 246 3079 Fragments arranged by size: 3079 1071 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5665 HpaI GTT'AAC Cuts at: 3255 3255 Size: 5665 KasI G'GCGC_C Cuts at: 2361 2522 2660 2687 2704 2800 2361 Size: 161 138 27 17 96 5226 Fragments arranged by size: 5226 161 138 96 27 17 KpnI G_GTAC'C Cuts at: 2990 2990 Size: 5665 MfeI C'AATT_G Cuts at: 3264 3264 Size: 5665 MluI A'CGCG_T Cuts at: 2598 5541 2598 Size: 2943 2722 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2619 2640 2649 4779 4963 1305 Size: 1314 21 9 2130 184 2007 Fragments arranged by size: 2130 2007 1314 184 21 9 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3843 Fragments arranged by size: 3843 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3623 3982 4141 518 Size: 648 2457 359 159 2042 Fragments arranged by size: 2457 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2204 2259 2280 2431 2554 2568 2586 2646 Size: 598 55 21 151 123 14 18 60 Cuts at: 2646 2886 3081 3758 4906 5151 1606 Size: 240 195 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 598 245 240 195 151 123 60 55 21 18 14 NarI GG'CG_CC Cuts at: 2362 2523 2661 2688 2705 2801 2362 Size: 161 138 27 17 96 5226 Fragments arranged by size: 5226 161 138 96 27 17 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4174 Fragments arranged by size: 4174 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5665 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5665 NotI GC'GGCC_GC Cuts at: 3112 3112 Size: 5665 NspI r_CATG'y Cuts at: 1132 1229 2246 3023 4568 1132 Size: 97 1017 777 1545 2229 Fragments arranged by size: 2229 1545 1017 777 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5665 PciI A'CATG_T Cuts at: 4564 4564 Size: 5665 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5665 PmeI GTTT'AAAC Cuts at: 2969 2969 Size: 5665 PmlI CAC'GTG Cuts at: 2161 2231 2161 Size: 70 5595 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3734 3766 5247 5279 3734 Size: 32 1481 32 4120 Fragments arranged by size: 4120 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5665 PsiI TTA'TAA Cuts at: 3235 3235 Size: 5665 PspOMI G'GGCC_C Cuts at: 2548 2548 Size: 5665 PstI C_TGCA'G Cuts at: 830 2487 3017 830 Size: 1657 530 3478 Fragments arranged by size: 3478 1657 530 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5665 SacII CC_GC'GG Cuts at: 2205 2569 2205 Size: 364 5301 SalI G'TCGA_C Cuts at: 1936 3007 1936 Size: 1071 4594 SapI GCTCTTCn'nnn_ Cuts at: 2178 2178 Size: 5665 SbfI CC_TGCA'GG Cuts at: 2487 3017 2487 Size: 530 5135 ScaI AGT'ACT Cuts at: 3913 5625 3913 Size: 1712 3953 SexAI A'CCwGG_T Cuts at: 2955 2955 Size: 5665 SfcI C'TryA_G Cuts at: 826 1045 2483 2581 2641 3013 4148 4829 5020 Size: 219 1438 98 60 372 1135 681 191 Cuts at: 5020 826 Size: 1471 Fragments arranged by size: 1471 1438 1135 681 372 219 191 98 60 SfoI GGC'GCC Cuts at: 2363 2524 2662 2689 2706 2802 2363 Size: 161 138 27 17 96 5226 Fragments arranged by size: 5226 161 138 96 27 17 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5665 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5665 SmaI CCC'GGG Cuts at: 1872 2223 2562 2725 2992 1872 Size: 351 339 163 267 4545 Fragments arranged by size: 4545 351 339 267 163 SmlI C'TyrA_G Cuts at: 820 1017 1943 2399 3769 4670 4932 820 Size: 197 926 456 1370 901 262 1553 Fragments arranged by size: 1553 1370 926 901 456 262 197 SnaBI TAC'GTA Cuts at: 493 3456 493 Size: 2963 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5665 SphI G_CATG'C Cuts at: 1132 3023 1132 Size: 1891 3774 SspI AAT'ATT Cuts at: 5 52 3589 5 Size: 47 3537 2081 Fragments arranged by size: 3537 2081 47 StuI AGG'CCT Cuts at: 1517 2387 1517 Size: 870 4795 StyI C'CwwG_G Cuts at: 513 1065 2004 2439 3104 513 Size: 552 939 435 665 3074 Fragments arranged by size: 3074 939 665 552 435 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2912 3692 3709 3862 4047 4492 325 Size: 1255 1332 780 17 153 185 445 1498 Fragments arranged by size: 1498 1332 1255 780 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3911 5623 96 Size: 274 80 33 51 1443 1934 1712 138 Fragments arranged by size: 1934 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5625 5657 5625 Size: 32 5633 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5665 XbaI T'CTAG_A Cuts at: 3001 3001 Size: 5665 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5665 XmaI C'CCGG_G Cuts at: 1870 2221 2560 2723 2990 1870 Size: 351 339 163 267 4545 Fragments arranged by size: 4545 351 339 267 163 XmnI GAAnn'nnTTC Cuts at: 3794 3794 Size: 5665 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3473 276 Size: 53 83 186 936 1939 2468 Fragments arranged by size: 2468 1939 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SfiI SrfI SwaI XcmI