(Circular) (Six-base) MAPSORT of: pFN21AB7611.seq Check: 3094 from: 1 to: 5611 pFN21AB7611 5611 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3421 278 Size: 53 83 186 936 1885 2468 Fragments arranged by size: 2468 1885 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2954 1937 Size: 1017 4594 Acc65I G'GTAC_C Cuts at: 2932 2932 Size: 5611 AclI AA'CG_TT Cuts at: 1563 3738 4111 1563 Size: 2175 373 3063 Fragments arranged by size: 3063 2175 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2818 3674 5058 5326 1632 Size: 1186 856 1384 268 1917 Fragments arranged by size: 1917 1384 1186 856 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5414 AflIII A'CryG_T Cuts at: 1203 4510 5487 1203 Size: 3307 977 1327 Fragments arranged by size: 3307 1327 977 AgeI A'CCGG_T Cuts at: 4442 4442 Size: 5611 AhdI GACnn_n'nnGTC Cuts at: 4340 4340 Size: 5611 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5611 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5611 AlwNI CAG_nnn'CTG Cuts at: 2066 2639 2656 3354 4926 2066 Size: 573 17 698 1572 2751 Fragments arranged by size: 2751 1572 698 573 17 ApaI G_GGCC'C Cuts at: 2647 2669 2647 Size: 22 5589 ApaLI G'TGCA_C Cuts at: 2849 3668 4824 2849 Size: 819 1156 3636 Fragments arranged by size: 3636 1156 819 ApoI r'AATT_y Cuts at: 1493 2920 3146 3298 5407 1493 Size: 1427 226 152 2109 1697 Fragments arranged by size: 2109 1697 1427 226 152 AscI GG'CGCG_CC Cuts at: 2344 2344 Size: 5611 AseI AT'TA_AT Cuts at: 160 1032 4165 160 Size: 872 3133 1606 Fragments arranged by size: 3133 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5611 AvaI C'yCGr_G Cuts at: 1870 1943 2414 2620 2672 2788 2936 1870 Size: 73 471 206 52 116 148 4545 Fragments arranged by size: 4545 471 206 148 116 73 52 BamHI G'GATC_C Cuts at: 2941 3308 2941 Size: 367 5244 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2407 2425 2599 2624 2932 Size: 325 217 627 620 18 174 25 308 Cuts at: 2932 4387 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 620 325 308 217 174 25 18 BanII G_rGCy'C Cuts at: 1391 2045 2647 2669 2930 1391 Size: 654 602 22 261 4072 Fragments arranged by size: 4072 654 602 261 22 BbeI G_GCGC'C Cuts at: 2411 2411 Size: 5611 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2557 928 Size: 971 658 3982 Fragments arranged by size: 3982 971 658 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2270 2304 3802 3836 1903 Size: 34 333 34 1498 34 3678 Fragments arranged by size: 3678 1498 333 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3503 4713 3503 Size: 1210 4401 BclI T'GATC_A Cuts at: 1548 1548 Size: 5611 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2744 4222 136 Size: 107 122 71 631 605 1072 1478 1525 Fragments arranged by size: 1525 1478 1072 631 605 122 107 71 BglII A'GATC_T Cuts at: 5606 5606 Size: 5611 BlpI GC'TnA_GC Cuts at: 2021 3028 2021 Size: 1007 4604 Bme1580I G_kGCm'C Cuts at: 1790 2604 2627 2647 2669 2853 3672 4828 1790 Size: 814 23 20 22 184 819 1156 2573 Fragments arranged by size: 2573 1156 819 814 184 23 22 20 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5611 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3342 4300 442 Size: 416 53 83 399 24 1925 958 1753 Fragments arranged by size: 1925 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5611 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4271 1239 Size: 3032 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3736 4601 4899 3736 Size: 865 298 4448 Fragments arranged by size: 4448 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4274 882 Size: 617 2775 2219 Fragments arranged by size: 2775 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3402 493 Size: 711 2198 2702 Fragments arranged by size: 2702 2198 711 BsaBI GATnn'nnATC Cuts at: 5605 5605 Size: 5611 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2408 3418 3800 4435 Size: 53 83 186 936 875 1010 382 635 Cuts at: 4435 275 Size: 1451 Fragments arranged by size: 1451 1010 936 875 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4044 4442 4716 4863 5274 4044 Size: 398 274 147 411 4381 Fragments arranged by size: 4381 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5386 Fragments arranged by size: 5386 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2297 2453 1186 Size: 1111 156 4344 Fragments arranged by size: 4344 1111 156 BseYI C'CCAG_C Cuts at: 2082 2576 4814 5310 2082 Size: 494 2238 496 2383 Fragments arranged by size: 2383 2238 496 494 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5038 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2688 2730 3061 3822 4850 1540 Size: 275 186 687 42 331 761 1028 2301 Fragments arranged by size: 2301 1028 761 687 331 275 186 42 BsiHKAI G_wGCw'C Cuts at: 2097 2118 2276 2853 3672 3757 4828 2097 Size: 21 158 577 819 85 1071 2880 Fragments arranged by size: 2880 1071 819 577 158 85 21 BsiWI C'GTAC_G Cuts at: 2460 2460 Size: 5611 BsmI GAATG_Cn' Cuts at: 3122 3215 3122 Size: 93 5518 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2097 2118 2276 2604 2627 2647 Size: 399 255 52 21 158 328 23 20 Cuts at: 2647 2669 2853 2930 3672 3757 4828 1391 Size: 22 184 77 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 328 255 184 158 85 77 52 23 22 21 20 BspEI T'CCGG_A Cuts at: 5274 5274 Size: 5611 BspHI T'CATG_A Cuts at: 3500 5230 3500 Size: 1730 3881 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2966 844 Size: 2122 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2413 2497 3498 1394 Size: 429 590 84 1001 3507 Fragments arranged by size: 3507 1001 590 429 84 BsrDI GCAATG_nn' Cuts at: 65 1248 4106 4280 65 Size: 1183 2858 174 1396 Fragments arranged by size: 2858 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2340 4255 4442 1700 Size: 110 530 1915 187 2869 Fragments arranged by size: 2869 1915 530 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5611 BssHII G'CGCG_C Cuts at: 1925 2344 1925 Size: 419 5192 BssSI C'ACGA_G Cuts at: 3671 4683 3671 Size: 1012 4599 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5611 BstBI TT'CG_AA Cuts at: 4415 4415 Size: 5611 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5611 BstYI r'GATC_y Cuts at: 1972 2941 3308 3693 3710 5162 5248 5260 5606 Size: 969 367 385 17 1452 86 12 346 Cuts at: 5606 1972 Size: 1977 Fragments arranged by size: 1977 1452 969 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2595 2610 2742 2895 513 Size: 552 939 591 15 132 153 3229 Fragments arranged by size: 3229 939 591 552 153 132 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2569 487 Size: 683 56 1343 3529 Fragments arranged by size: 3529 1343 683 56 BtsI GCAGTG_nn' Cuts at: 3127 3921 3941 3127 Size: 794 20 4797 Fragments arranged by size: 4797 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5611 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5576 DraI TTT'AAA Cuts at: 2915 2915 Size: 5611 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4618 809 Size: 507 3302 1802 Fragments arranged by size: 3302 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2471 2685 2727 Size: 54 1425 50 275 18 641 214 42 Cuts at: 2727 2796 3058 3947 5576 8 Size: 69 262 889 1629 43 Fragments arranged by size: 1629 1425 889 641 275 262 214 69 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 2685 2727 3058 1537 Size: 275 873 42 331 4090 Fragments arranged by size: 4090 873 331 275 42 EarI CTCTTCn'nnn_ Cuts at: 1353 2761 3541 1353 Size: 1408 780 3423 Fragments arranged by size: 3423 1408 780 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2371 2596 4198 4572 4718 387 Size: 1064 138 782 225 1602 374 146 1280 Fragments arranged by size: 1602 1280 1064 782 374 225 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2818 3674 4271 5058 5326 1239 Size: 393 1186 856 597 787 268 1524 Fragments arranged by size: 1524 1186 856 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2248 2309 2248 Size: 61 5550 EcoO109I rG'GnC_Cy Cuts at: 2543 2636 2643 2644 2653 2665 2666 2776 2543 Size: 93 7 1 9 12 1 110 5378 Fragments arranged by size: 5378 110 93 12 9 7 1 1 EcoRI G'AATT_C Cuts at: 2920 3298 2920 Size: 378 5233 FspI TGC'GCA Cuts at: 4117 4117 Size: 5611 HaeII r_GCGC'y Cuts at: 1678 2411 4758 1678 Size: 733 2347 2531 Fragments arranged by size: 2531 2347 733 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4252 4284 4326 4358 1373 Size: 32 135 32 2680 32 42 32 2626 Fragments arranged by size: 2680 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2536 2955 3201 669 Size: 988 281 598 419 246 3079 Fragments arranged by size: 3079 988 598 419 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5611 HpaI GTT'AAC Cuts at: 3201 3201 Size: 5611 KasI G'GCGC_C Cuts at: 2407 2407 Size: 5611 KpnI G_GTAC'C Cuts at: 2936 2936 Size: 5611 MfeI C'AATT_G Cuts at: 3210 3210 Size: 5611 MluI A'CGCG_T Cuts at: 5487 5487 Size: 5611 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4725 4909 1305 Size: 3420 184 2007 Fragments arranged by size: 3420 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2798 10 Size: 54 1425 343 966 2823 Fragments arranged by size: 2823 1425 966 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3569 3928 4087 518 Size: 648 2403 359 159 2042 Fragments arranged by size: 2403 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2191 2612 2744 2846 3027 3704 4852 5097 Size: 585 421 132 102 181 677 1148 245 Cuts at: 5097 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 585 421 245 181 132 102 NarI GG'CG_CC Cuts at: 2408 2408 Size: 5611 NcoI C'CATG_G Cuts at: 513 1065 2004 2595 513 Size: 552 939 591 3529 Fragments arranged by size: 3529 939 591 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5611 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5611 NotI GC'GGCC_GC Cuts at: 3058 3058 Size: 5611 NspI r_CATG'y Cuts at: 1132 1229 2969 4514 1132 Size: 97 1740 1545 2229 Fragments arranged by size: 2229 1740 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5611 PciI A'CATG_T Cuts at: 4510 4510 Size: 5611 PflMI CCAn_nnn'nTGG Cuts at: 1108 2639 1108 Size: 1531 4080 PmeI GTTT'AAAC Cuts at: 2915 2915 Size: 5611 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3680 3712 5193 5225 3680 Size: 32 1481 32 4066 Fragments arranged by size: 4066 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2776 2776 Size: 5611 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5611 PsiI TTA'TAA Cuts at: 3181 3181 Size: 5611 PspOMI G'GGCC_C Cuts at: 2643 2665 2643 Size: 22 5589 PspXI vC'TCGA_Gb Cuts at: 2788 2788 Size: 5611 PstI C_TGCA'G Cuts at: 830 2652 2963 830 Size: 1822 311 3478 Fragments arranged by size: 3478 1822 311 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5611 SacII CC_GC'GG Cuts at: 2613 2745 2613 Size: 132 5479 SalI G'TCGA_C Cuts at: 1936 2953 1936 Size: 1017 4594 SanDI GG'GwC_CC Cuts at: 2776 2776 Size: 5611 SbfI CC_TGCA'GG Cuts at: 2652 2963 2652 Size: 311 5300 ScaI AGT'ACT Cuts at: 3859 5571 3859 Size: 1712 3899 SfcI C'TryA_G Cuts at: 826 1045 2225 2648 2959 4094 4775 4966 826 Size: 219 1180 423 311 1135 681 191 1471 Fragments arranged by size: 1471 1180 1135 681 423 311 219 191 SfoI GGC'GCC Cuts at: 2409 2409 Size: 5611 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5611 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5611 SmaI CCC'GGG Cuts at: 1872 2622 2674 2938 1872 Size: 750 52 264 4545 Fragments arranged by size: 4545 750 264 52 SmlI C'TyrA_G Cuts at: 820 1017 1943 2788 3715 4616 4878 820 Size: 197 926 845 927 901 262 1553 Fragments arranged by size: 1553 927 926 901 845 262 197 SnaBI TAC'GTA Cuts at: 493 3402 493 Size: 2909 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5611 SphI G_CATG'C Cuts at: 1132 2969 1132 Size: 1837 3774 SspI AAT'ATT Cuts at: 5 52 3535 5 Size: 47 3483 2081 Fragments arranged by size: 3483 2081 47 StuI AGG'CCT Cuts at: 1517 2135 1517 Size: 618 4993 StyI C'CwwG_G Cuts at: 513 1065 2004 2478 2595 3050 513 Size: 552 939 474 117 455 3074 Fragments arranged by size: 3074 939 552 474 455 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2521 2590 3638 3655 3808 3993 4438 Size: 1255 941 69 1048 17 153 185 445 Cuts at: 4438 325 Size: 1498 Fragments arranged by size: 1498 1255 1048 941 445 185 153 69 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3857 5569 96 Size: 274 80 33 51 1443 1880 1712 138 Fragments arranged by size: 1880 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5571 5603 5571 Size: 32 5579 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5611 XbaI T'CTAG_A Cuts at: 2947 2947 Size: 5611 XhoI C'TCGA_G Cuts at: 1943 2788 1943 Size: 845 4766 XmaI C'CCGG_G Cuts at: 1870 2620 2672 2936 1870 Size: 750 52 264 4545 Fragments arranged by size: 4545 750 264 52 XmnI GAAnn'nnTTC Cuts at: 3740 3740 Size: 5611 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3419 276 Size: 53 83 186 936 1885 2468 Fragments arranged by size: 2468 1885 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PsrI PvuII RsrII SacI SapI SexAI SfiI SrfI SwaI XcmI