Restriction map for
pFN21AB7611
(Linear) (Six-base) MAPSORT of: KIBB7611.seq Check: 7862 from: 1 to: 906
KIBB7611.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 813 906
Size: 813 93
AlwNI CAG_nnn'CTG
Cuts at: 0 61 634 651 906
Size: 61 573 17 255
Fragments arranged by size:
573 255 61 17
ApaI G_GGCC'C
Cuts at: 0 642 664 906
Size: 642 22 242
Fragments arranged by size:
642 242 22
ApaLI G'TGCA_C
Cuts at: 0 844 906
Size: 844 62
AscI GG'CGCG_CC
Cuts at: 0 339 906
Size: 339 567
AvaI C'yCGr_G
Cuts at: 0 409 615 667 783 906
Size: 409 206 52 116 123
Fragments arranged by size:
409 206 123 116 52
BanI G'GyrC_C
Cuts at: 0 402 420 594 619 906
Size: 402 18 174 25 287
Fragments arranged by size:
402 287 174 25 18
BanII G_rGCy'C
Cuts at: 0 40 642 664 906
Size: 40 602 22 242
Fragments arranged by size:
602 242 40 22
BbeI G_GCGC'C
Cuts at: 0 406 906
Size: 406 500
BbsI GAAGACnn'nnnn_
Cuts at: 0 552 906
Size: 552 354
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 265 299 906
Size: 265 34 607
Fragments arranged by size:
607 265 34
BglI GCCn_nnn'nGGC
Cuts at: 0 739 906
Size: 739 167
BlpI GC'TnA_GC
Cuts at: 0 16 906
Size: 16 890
Bme1580I G_kGCm'C
Cuts at: 0 599 622 642 664 848 906
Size: 599 23 20 22 184 58
Fragments arranged by size:
599 184 58 23 22 20
BsaHI Gr'CG_yC
Cuts at: 0 403 906
Size: 403 503
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 448 906
Size: 292 156 458
Fragments arranged by size:
458 292 156
BseYI C'CCAG_C
Cuts at: 0 77 571 906
Size: 77 494 335
Fragments arranged by size:
494 335 77
BsiEI CG_ry'CG
Cuts at: 0 683 725 906
Size: 683 42 181
Fragments arranged by size:
683 181 42
BsiHKAI G_wGCw'C
Cuts at: 0 92 113 271 848 906
Size: 92 21 158 577 58
Fragments arranged by size:
577 158 92 58 21
BsiWI C'GTAC_G
Cuts at: 0 455 906
Size: 455 451
Bsp1286I G_dGCh'C
Cuts at: 0 40 92 113 271 599 622 642 664
Size: 40 52 21 158 328 23 20 22
Cuts at: 664 848 906
Size: 184 58
Fragments arranged by size:
328 184 158 58 52 40 23 22
21 20
BsrBI CCG'CTC
Cuts at: 0 408 492 906
Size: 408 84 414
Fragments arranged by size:
414 408 84
BsrFI r'CCGG_y
Cuts at: 0 335 906
Size: 335 571
BssHII G'CGCG_C
Cuts at: 0 339 906
Size: 339 567
BtgI C'CryG_G
Cuts at: 0 590 605 737 890 906
Size: 590 15 132 153 16
Fragments arranged by size:
590 153 132 16 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 564 906
Size: 564 342
EaeI y'GGCC_r
Cuts at: 0 466 680 722 791 906
Size: 466 214 42 69 115
Fragments arranged by size:
466 214 115 69 42
EagI C'GGCC_G
Cuts at: 0 680 722 906
Size: 680 42 184
Fragments arranged by size:
680 184 42
EarI CTCTTCn'nnn_
Cuts at: 0 756 906
Size: 756 150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 366 591 906
Size: 366 225 315
Fragments arranged by size:
366 315 225
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 813 906
Size: 813 93
EcoNI CCTnn'n_nnAGG
Cuts at: 0 243 304 906
Size: 243 61 602
Fragments arranged by size:
602 243 61
EcoO109I rG'GnC_Cy
Cuts at: 0 538 631 638 639 648 660 661 771
Size: 538 93 7 1 9 12 1 110
Cuts at: 771 906
Size: 135
Fragments arranged by size:
538 135 110 93 12 9 7 1
1
HaeII r_GCGC'y
Cuts at: 0 406 906
Size: 406 500
HincII GTy'rAC
Cuts at: 0 531 906
Size: 531 375
KasI G'GCGC_C
Cuts at: 0 402 906
Size: 402 504
MscI TGG'CCA
Cuts at: 0 793 906
Size: 793 113
MspA1I CmG'CkG
Cuts at: 0 186 607 739 841 906
Size: 186 421 132 102 65
Fragments arranged by size:
421 186 132 102 65
NarI GG'CG_CC
Cuts at: 0 403 906
Size: 403 503
NcoI C'CATG_G
Cuts at: 0 590 906
Size: 590 316
PflMI CCAn_nnn'nTGG
Cuts at: 0 634 906
Size: 634 272
PpuMI rG'GwC_Cy
Cuts at: 0 771 906
Size: 771 135
PspOMI G'GGCC_C
Cuts at: 0 638 660 906
Size: 638 22 246
Fragments arranged by size:
638 246 22
PspXI vC'TCGA_Gb
Cuts at: 0 783 906
Size: 783 123
PstI C_TGCA'G
Cuts at: 0 647 906
Size: 647 259
SacII CC_GC'GG
Cuts at: 0 608 740 906
Size: 608 132 166
Fragments arranged by size:
608 166 132
SanDI GG'GwC_CC
Cuts at: 0 771 906
Size: 771 135
SbfI CC_TGCA'GG
Cuts at: 0 647 906
Size: 647 259
SfcI C'TryA_G
Cuts at: 0 220 643 906
Size: 220 423 263
Fragments arranged by size:
423 263 220
SfoI GGC'GCC
Cuts at: 0 404 906
Size: 404 502
SmaI CCC'GGG
Cuts at: 0 617 669 906
Size: 617 52 237
Fragments arranged by size:
617 237 52
SmlI C'TyrA_G
Cuts at: 0 783 906
Size: 783 123
StuI AGG'CCT
Cuts at: 0 130 906
Size: 130 776
StyI C'CwwG_G
Cuts at: 0 473 590 906
Size: 473 117 316
Fragments arranged by size:
473 316 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 516 585 906
Size: 516 69 321
Fragments arranged by size:
516 321 69
XhoI C'TCGA_G
Cuts at: 0 783 906
Size: 783 123
XmaI C'CCGG_G
Cuts at: 0 615 667 906
Size: 615 52 239
Fragments arranged by size:
615 239 52
Enzymes that do cut:
AcuI AlwNI ApaI ApaLI AscI AvaI BanI BanII
BbeI BbsI BcgI BglI BlpI Bme1580I BsaHI BseRI
BseYI BsiEI BsiHKAI BsiWI Bsp1286I BsrBI BsrFI BssHII
BtgI BtgZI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I HaeII HincII KasI MscI MspA1I NarI NcoI
PflMI PpuMI PspOMI PspXI PstI SacII SanDI SbfI
SfcI SfoI SmaI SmlI StuI StyI TaqII XhoI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApoI AseI AsiSI
AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglII
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BsgI BsmI BsmBI BspEI
BspHI BspMI BsrDI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI
DraIII DrdI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HindIII HpaI KpnI MfeI MluI MmeI
MslI NaeI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PsrI PvuI PvuII RsrII SacI SalI
SapI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TatI TstI Tth111I XbaI
XcmI XmnI ZraI