Restriction map for
pFN21AB7595
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstF5I
BstUI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco52I Eco81I EcoRI EcoRV EcoT22I
FauI FbaI FokI FseI FspI
HgaI HindIII HpaI KpnI KspI
MaeI MamI MfeI MflI MluI
MroI MseI MunI MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I Tth111I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 676 GTCTAC GT^[AC][GT]AC
AcyI 127 GGCGCC G[GA]^CG[CT]C
AfaI 393 GTAC GT^AC
AlwNI 307 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 625 AGCGCT AGC^GCT
ApaI 67 GGGCCC GGGCC^C
AvaI 249 CCCGAG C^[CT]CG[GA]G
BalI 338 TGGCCA TGG^CCA
BbeI 127 GGCGCC GGCGC^C
BsaHI 127 GGCGCC G[GA]^CG[CT]C
BsaI 538 GAGACC GGTCTC(1/5)
BsgI 151 CTGCAC GTGCAG(16/14)
BsmBI 480 CGTCTC CGTCTC(1/5)
BsoBI 249 CCCGAG C^[CT]CG[GA]G
BspMI 672 GCAGGT ACCTGC(4/8)
BsrDI 690 CATTGC GCAATG(2/0)
BsrI 306 CCAGT ACTGG(1/-1)
BstEII 613 GGTGACC G^GT[ACGT]ACC
BstPI 613 GGTGACC G^GT[ACGT]ACC
Eco47III 625 AGCGCT AGC^GCT
EcoO65I 613 GGTGACC G^GT[ACGT]ACC
Hin1I 127 GGCGCC G[GA]^CG[CT]C
HincII 499 GTCAAC GT[CT]^[GA]AC
HindII 499 GTCAAC GT[CT]^[GA]AC
KasI 127 GGCGCC G^GCGCC
MaeII 26 ACGT A^CGT
MluNI 338 TGGCCA TGG^CCA
MscI 338 TGGCCA TGG^CCA
MslI 441 CACCCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 127 GGCGCC GG^CGCC
PshAI 492 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 393 GTAC GT^AC
SapI 138 GCTCTTC GCTCTTC(1/4)
SfaNI 473 GCATC GCATC(5/9)
SfcI 263 CTGTAG C^T[GA][CT]AG
SfoI 127 GGCGCC GGC^GCC
SmlI 287 CTCAAG C^T[CT][GA]AG
XcmI 326 CCAATGCCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AlwI 18 GATCC GGATC(4/5)
686 GGATC
AspHI 3 GAGCAC G[AT]GC[AT]^C
448 GTGCTC
AvaII 195 GGACC G^G[AT]CC
411 GGTCC
BanI 127 GGCGCC G^G[CT][GA]CC
375 GGTGCC
BbsI 57 GAAGAC GAAGAC(2/6)
595 GTCTTC
BcnI 21 CCGGG CC^[GC]GG
321 CCGGG
BglI 37 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
331 GCCCTCCTGGC
BpuAI 57 GAAGAC GAAGAC(2/6)
595 GTCTTC
BsiHKAI 3 GAGCAC G[AT]GC[AT]^C
448 GTGCTC
BsmAI 538 GAGAC GTCTC(1/5)
481 GTCTC
BsmFI 23 GGGAC GGGAC(10/14)
194 GGGAC
BstXI 341 CCAATGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
578 CCATCTATCTGG
EaeI 35 TGGCCG [CT]^GGCC[GA]
338 TGGCCA
EcoNI 414 CCTCTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
527 CCTGCCAGAGG
EcoT14I 426 CCAAGG C^C[AT][AT]GG
494 CCAAGG
HapII 21 CCGG C^CGG
321 CCGG
HinfI 620 GACTC G^A[ACGT]TC
664 GAGTC
HpaII 21 CCGG C^CGG
321 CCGG
HphI 613 GGTGA GGTGA(8/7)
455 TCACC
MaeIII 254 GTGAC ^GT[ACGT]AC
614 GTGAC
MspA1I 367 CAGCTG C[AC]G^C[GT]G
601 CAGCTG
MspI 21 CCGG C^CGG
321 CCGG
NciI 21 CCGGG CC^[GC]GG
321 CCGGG
PflMI 366 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
560 CCAAGCCCTGG
PleI 620 GACTC GAGTC(4/5)
664 GAGTC
PpuMI 194 GGGACCT [GA]G^G[AT]CC[CT]
410 AGGTCCT
PvuII 367 CAGCTG CAG^CTG
601 CAGCTG
StyI 426 CCAAGG C^C[AT][AT]GG
494 CCAAGG
TaqI 243 TCGA T^CGA
653 TCGA
Tsp45I 254 GTGAC ^GT[GC]AC
614 GTGAC
TspRI 6 CACTG CA[GC]TG[ACGT][ACGT]^
307 CAGTG
TthHB8I 243 TCGA T^CGA
653 TCGA
Van91I 366 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
560 CCAAGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI BanII BbvCI BbvI
BmyI BpmI BsaJI BseRI BsiYI
BslI Bsp1286I BssKI BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII Fnu4HI HaeII
HaeIII HhaI HinP1I ItaI Ksp632I
MboI MboII MnlI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI TseI