Restriction map for
pFN21AB7587
(Linear) (Six-base) MAPSORT of: KIBB7587.seq Check: 9851 from: 1 to: 672
KIBB7587.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 672
Size: 331 341
AlwNI CAG_nnn'CTG
Cuts at: 0 195 345 402 672
Size: 195 150 57 270
Fragments arranged by size:
270 195 150 57
ApaI G_GGCC'C
Cuts at: 0 146 461 672
Size: 146 315 211
Fragments arranged by size:
315 211 146
ApaLI G'TGCA_C
Cuts at: 0 524 672
Size: 524 148
AvaI C'yCGr_G
Cuts at: 0 130 172 672
Size: 130 42 500
Fragments arranged by size:
500 130 42
BanI G'GyrC_C
Cuts at: 0 471 672
Size: 471 201
BanII G_rGCy'C
Cuts at: 0 119 130 146 461 672
Size: 119 11 16 315 211
Fragments arranged by size:
315 211 119 16 11
BglI GCCn_nnn'nGGC
Cuts at: 0 304 672
Size: 304 368
BlpI GC'TnA_GC
Cuts at: 0 406 672
Size: 406 266
Bme1580I G_kGCm'C
Cuts at: 0 146 461 476 528 672
Size: 146 315 15 52 144
Fragments arranged by size:
315 146 144 52 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 379 550 672
Size: 64 315 171 122
Fragments arranged by size:
315 171 122 64
Bpu10I CC'TnA_GC
Cuts at: 0 124 672
Size: 124 548
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 265 672
Size: 169 96 407
Fragments arranged by size:
407 169 96
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 484 514 580 610 672
Size: 484 30 66 30 62
Fragments arranged by size:
484 66 62 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 439 672
Size: 439 233
BseYI C'CCAG_C
Cuts at: 0 162 461 475 672
Size: 162 299 14 197
Fragments arranged by size:
299 197 162 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 672
Size: 577 95
BsiHKAI G_wGCw'C
Cuts at: 0 119 179 528 672
Size: 119 60 349 144
Fragments arranged by size:
349 144 119 60
BsmI GAATG_Cn'
Cuts at: 0 487 672
Size: 487 185
Bsp1286I G_dGCh'C
Cuts at: 0 119 130 146 179 461 476 528 672
Size: 119 11 16 33 282 15 52 144
Fragments arranged by size:
282 144 119 52 33 16 15 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 74 599 672
Size: 74 525 73
Fragments arranged by size:
525 74 73
BsrBI CCG'CTC
Cuts at: 0 108 672
Size: 108 564
BsrFI r'CCGG_y
Cuts at: 0 242 672
Size: 242 430
BstBI TT'CG_AA
Cuts at: 0 89 672
Size: 89 583
BstYI r'GATC_y
Cuts at: 0 359 672
Size: 359 313
BtgI C'CryG_G
Cuts at: 0 199 236 672
Size: 199 37 436
Fragments arranged by size:
436 199 37
BtsI GCAGTG_nn'
Cuts at: 0 375 528 672
Size: 375 153 144
Fragments arranged by size:
375 153 144
EaeI y'GGCC_r
Cuts at: 0 254 672
Size: 254 418
EarI CTCTTCn'nnn_
Cuts at: 0 307 672
Size: 307 365
EcoICRI GAG'CTC
Cuts at: 0 117 672
Size: 117 555
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 331 379 550 672
Size: 64 267 48 171 122
Fragments arranged by size:
267 171 122 64 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 418 672
Size: 418 254
EcoO109I rG'GnC_Cy
Cuts at: 0 135 142 143 167 399 458 672
Size: 135 7 1 24 232 59 214
Fragments arranged by size:
232 214 135 59 24 7 1
FspI TGC'GCA
Cuts at: 0 367 672
Size: 367 305
HaeII r_GCGC'y
Cuts at: 0 393 500 542 672
Size: 393 107 42 130
Fragments arranged by size:
393 130 107 42
MslI CAynn'nnrTG
Cuts at: 0 625 672
Size: 625 47
MspA1I CmG'CkG
Cuts at: 0 342 672
Size: 342 330
PasI CC'CwG_GG
Cuts at: 0 139 547 672
Size: 139 408 125
Fragments arranged by size:
408 139 125
PpuMI rG'GwC_Cy
Cuts at: 0 167 672
Size: 167 505
PspOMI G'GGCC_C
Cuts at: 0 142 457 672
Size: 142 315 215
Fragments arranged by size:
315 215 142
PstI C_TGCA'G
Cuts at: 0 508 672
Size: 508 164
SacI G_AGCT'C
Cuts at: 0 119 672
Size: 119 553
SanDI GG'GwC_CC
Cuts at: 0 167 672
Size: 167 505
SfcI C'TryA_G
Cuts at: 0 504 575 672
Size: 504 71 97
Fragments arranged by size:
504 97 71
SmlI C'TyrA_G
Cuts at: 0 148 280 672
Size: 148 132 392
Fragments arranged by size:
392 148 132
StuI AGG'CCT
Cuts at: 0 298 672
Size: 298 374
StyI C'CwwG_G
Cuts at: 0 350 672
Size: 350 322
TatI w'GTAC_w
Cuts at: 0 380 672
Size: 380 292
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 484 516 672
Size: 484 32 156
Fragments arranged by size:
484 156 32
Enzymes that do cut:
AcuI AlwNI ApaI ApaLI AvaI BanI BanII BglI
BlpI Bme1580I BpmI Bpu10I BpuEI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BstBI
BstYI BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I FspI HaeII MslI MspA1I PasI PpuMI PspOMI
PstI SacI SanDI SfcI SmlI StuI StyI TatI
TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BmgBI BmrI BmtI BplI
BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI
BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoRI EcoRV FalI FseI FspAI
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TaqII Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI