Restriction map for
pFN21AB7569
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BpmI BsaAI BsaHI BsaI
BsaWI BseAI BsgI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I CfoI Cfr10I
ClaI CpoI DraI DraIII DsaI
Eam1105I EarI Eco47III EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HphI KasI KpnI Ksp632I KspI
MaeI MaeII MaeIII MfeI MluI
MluNI MroI MscI MunI MvnI
MwoI NaeI NarI NcoI NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp45I
Tsp509I Tth111I Van91I XbaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AocI 388 CCTAAGG CC^T[ACGT]AGG
AspHI 169 GAGCTC G[AT]GC[AT]^C
AvaII 78 GGACC G^G[AT]CC
BanI 427 GGCACC G^G[CT][GA]CC
BanII 169 GAGCTC G[GA]GC[CT]^C
BbsI 214 GAAGAC GAAGAC(2/6)
BlpI 377 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 377 GCTCAGC GC^T[ACGT]AGC
BpuAI 214 GAAGAC GAAGAC(2/6)
BsaBI 315 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 343 GAGGAG GAGGAG(10/8)
BsiEI 62 CGGCCG CG[GA][CT]^CG
BsiHKAI 169 GAGCTC G[AT]GC[AT]^C
BsmFI 77 GGGAC GGGAC(10/14)
BsrBI 375 CCGCTC CCGCTC(-3/-3)
BssSI 13 CACGAG CACGAG(-5/-1)
BstNI 324 CCTGG CC^[AT]GG
BstYI 596 AGATCC [GA]^GATC[CT]
Bsu36I 388 CCTAAGG CC^T[ACGT]AGG
Cac8I 167 GCGAGC GC[ACGT]^[ACGT]GC
CelII 377 GCTCAGC GC^T[ACGT]AGC
Cfr13I 78 GGACC G^G[ACGT]CC
DraII 77 GGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 42 GACAGGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 62 CGGCCG [CT]^GGCC[GA]
EagI 62 CGGCCG C^GGCCG
EclXI 62 CGGCCG C^GGCCG
Eco52I 62 CGGCCG C^GGCCG
Eco81I 388 CCTAAGG CC^T[ACGT]AGG
EcoO109I 77 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRII 324 CCTGG ^CC[AT]GG
EcoT14I 473 CCAAGG C^C[AT][AT]GG
HaeIII 63 GGCC GG^CC
HgaI 127 GACGC GACGC(5/10)
MamI 315 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 596 AGATCC [GA]^GATC[CT]
MslI 429 CACCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 324 CCTGG CC^[AT]GG
NdeI 556 CATATG CA^TATG
PaeR7I 172 CTCGAG C^TCGAG
PpuMI 77 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 349 CAGCTG CAG^CTG
SacI 169 GAGCTC GAGCT^C
Sau96I 78 GGACC G^G[ACGT]CC
SmlI 172 CTCGAG C^T[CT][GA]AG
StyI 473 CCAAGG C^C[AT][AT]GG
XcmI 414 CCAGTCCATCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 172 CTCGAG C^TCGAG
XhoII 596 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 411 GATCC GGATC(4/5)
597 GATCC
BmyI 70 GTGCCC G[AGT]GC[ACT]^C
169 GAGCTC
BsiYI 398 CCATCAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
569 CCGTTAGAAGG
BslI 398 CCATCAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
569 CCGTTAGAAGG
BsmBI 58 GAGACG CGTCTC(1/5)
125 GAGACG
Bsp1286I 70 GTGCCC G[AGT]GC[ACT]^C
169 GAGCTC
DpnII 411 GATC ^GATC
597 GATC
HapII 74 CCGG C^CGG
82 CCGG
HpaII 74 CCGG C^CGG
82 CCGG
MboI 411 GATC ^GATC
597 GATC
MspI 74 CCGG C^CGG
82 CCGG
NdeII 411 GATC ^GATC
597 GATC
PstI 152 CTGCAG CTGCA^G
236 CTGCAG
Sau3AI 411 GATC ^GATC
597 GATC
SfaNI 7 GATGC GCATC(5/9)
315 GATGC
SfcI 152 CTGCAG C^T[GA][CT]AG
236 CTGCAG
SmaI 73 CCCGGG CCC^GGG
81 CCCGGG
TaqI 173 TCGA T^CGA
607 TCGA
TfiI 104 GAATC G^A[AT]TC
306 GATTC
TspRI 423 CACTG CA[GC]TG[ACGT][ACGT]^
506 CACTG
TthHB8I 173 TCGA T^CGA
607 TCGA
XmaCI 73 CCCGGG C^CCGGG
81 CCCGGG
XmaI 73 CCCGGG C^CCGGG
81 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI AvaI BbvI BcnI
BsaJI BsmAI BsoBI BsrI BssKI
BstF5I DdeI Fnu4HI FokI HinfI
ItaI MboII MnlI MseI MspA1I
NciI NlaIV PleI ScrFI Tru9I
TseI