Restriction map for
pFN21AB7569
(Linear) (Six-base) MAPSORT of: KIBB7569.seq Check: 7900 from: 1 to: 633
KIBB7569.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AleI CACnn'nnGTG
Cuts at: 0 433 633
Size: 433 200
AvaI C'yCGr_G
Cuts at: 0 73 81 172 633
Size: 73 8 91 461
Fragments arranged by size:
461 91 73 8
BanI G'GyrC_C
Cuts at: 0 427 633
Size: 427 206
BanII G_rGCy'C
Cuts at: 0 173 633
Size: 173 460
BbsI GAAGACnn'nnnn_
Cuts at: 0 221 633
Size: 221 412
BlpI GC'TnA_GC
Cuts at: 0 378 633
Size: 378 255
Bme1580I G_kGCm'C
Cuts at: 0 74 633
Size: 74 559
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 39 633
Size: 39 594
BsaBI GATnn'nnATC
Cuts at: 0 319 633
Size: 319 314
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 358 633
Size: 358 275
BsiEI CG_ry'CG
Cuts at: 0 65 633
Size: 65 568
BsiHKAI G_wGCw'C
Cuts at: 0 173 633
Size: 173 460
BsmBI CGTCTCn'nnnn_
Cuts at: 0 52 119 633
Size: 52 67 514
Fragments arranged by size:
514 67 52
Bsp1286I G_dGCh'C
Cuts at: 0 74 173 633
Size: 74 99 460
Fragments arranged by size:
460 99 74
BsrBI CCG'CTC
Cuts at: 0 377 633
Size: 377 256
BssSI C'ACGA_G
Cuts at: 0 13 633
Size: 13 620
BstYI r'GATC_y
Cuts at: 0 596 633
Size: 596 37
Bsu36I CC'TnA_GG
Cuts at: 0 389 633
Size: 389 244
DrdI GACnn_nn'nnGTC
Cuts at: 0 48 633
Size: 48 585
EaeI y'GGCC_r
Cuts at: 0 62 633
Size: 62 571
EagI C'GGCC_G
Cuts at: 0 62 633
Size: 62 571
EcoICRI GAG'CTC
Cuts at: 0 171 633
Size: 171 462
EcoO109I rG'GnC_Cy
Cuts at: 0 78 633
Size: 78 555
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 50 633
Size: 32 18 583
Fragments arranged by size:
583 32 18
MslI CAynn'nnrTG
Cuts at: 0 433 633
Size: 433 200
MspA1I CmG'CkG
Cuts at: 0 67 157 351 633
Size: 67 90 194 282
Fragments arranged by size:
282 194 90 67
NdeI CA'TA_TG
Cuts at: 0 557 633
Size: 557 76
PpuMI rG'GwC_Cy
Cuts at: 0 78 633
Size: 78 555
PstI C_TGCA'G
Cuts at: 0 156 240 633
Size: 156 84 393
Fragments arranged by size:
393 156 84
PvuII CAG'CTG
Cuts at: 0 351 633
Size: 351 282
SacI G_AGCT'C
Cuts at: 0 173 633
Size: 173 460
SanDI GG'GwC_CC
Cuts at: 0 78 633
Size: 78 555
SfcI C'TryA_G
Cuts at: 0 152 236 633
Size: 152 84 397
Fragments arranged by size:
397 152 84
SmaI CCC'GGG
Cuts at: 0 75 83 633
Size: 75 8 550
Fragments arranged by size:
550 75 8
SmlI C'TyrA_G
Cuts at: 0 172 633
Size: 172 461
StyI C'CwwG_G
Cuts at: 0 473 633
Size: 473 160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 421 633
Size: 421 212
XhoI C'TCGA_G
Cuts at: 0 172 633
Size: 172 461
XmaI C'CCGG_G
Cuts at: 0 73 81 633
Size: 73 8 552
Fragments arranged by size:
552 73 8
Enzymes that do cut:
AleI AvaI BanI BanII BbsI BlpI Bme1580I BplI
BsaBI BseRI BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BssSI
BstYI Bsu36I DrdI EaeI EagI EcoICRI EcoO109I MmeI
MslI MspA1I NdeI PpuMI PstI PvuII SacI SanDI
SfcI SmaI SmlI StyI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseYI BsgI BsiWI BsmI BspEI BspHI
BspMI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstXI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
TstI Tth111I XbaI XmnI ZraI