Restriction map for
pFN21AB7559
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AlwNI
AocI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BsaBI BsaHI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI EagI EarI EclXI Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I MaeI MamI MfeI
MflI MluI MseI MslI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccIII 24 TCCGGA T^CCGGA
AfaI 60 GTAC GT^AC
AhdI 127 GACAACTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 517 GTGCAC G^TGCAC
AlwI 22 GATCC GGATC(4/5)
Aor51HI 170 AGCGCT AGC^GCT
ApaLI 517 GTGCAC G^TGCAC
AspEI 127 GACAACTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 517 GTGCAC G[AT]GC[AT]^C
AvaI 552 CTCGGG C^[CT]CG[GA]G
BalI 538 TGGCCA TGG^CCA
BanI 408 GGTGCC G^G[CT][GA]CC
BbsI 557 GAAGAC GAAGAC(2/6)
BpmI 381 CTCCAG CTGGAG(16/14)
BpuAI 557 GAAGAC GAAGAC(2/6)
BsaAI 514 TACGTG [CT]AC^GT[GA]
BsaI 549 GGTCTC GGTCTC(1/5)
BseAI 24 TCCGGA T^CCGGA
BsiHKAI 517 GTGCAC G[AT]GC[AT]^C
BsmAI 550 GTCTC GTCTC(1/5)
BsmFI 67 GTCCC GGGAC(10/14)
BsoBI 552 CTCGGG C^[CT]CG[GA]G
BspEI 24 TCCGGA T^CCGGA
BsrFI 190 GCCGGC [GA]^CCGG[CT]
BstAPI 480 GCAGCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 356 GGATG GGATG(2/0)
Cfr10I 190 GCCGGC [GA]^CCGG[CT]
DpnII 22 GATC ^GATC
DraII 149 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 520 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 127 GACAACTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 170 AGCGCT AGC^GCT
EcoO109I 149 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 290 CCAAGG C^C[AT][AT]GG
FokI 356 GGATG GGATG(9/13)
HaeII 170 AGCGCT [GA]GCGC^[CT]
HinfI 565 GACTC G^A[ACGT]TC
KspI 93 CCGCGG CCGC^GG
MaeII 515 ACGT A^CGT
MboI 22 GATC ^GATC
MboII 557 GAAGA GAAGA(8/7)
MluNI 538 TGGCCA TGG^CCA
MroI 24 TCCGGA T^CCGGA
MscI 538 TGGCCA TGG^CCA
NaeI 190 GCCGGC GCC^GGC
NdeII 22 GATC ^GATC
NgoMIV 190 GCCGGC G^CCGGC
NlaIII 536 CATG CATG^
PleI 565 GACTC GAGTC(4/5)
PpuMI 149 AGGACCC [GA]G^G[AT]CC[CT]
PstI 49 CTGCAG CTGCA^G
PvuII 481 CAGCTG CAG^CTG
RsaI 60 GTAC GT^AC
SacII 93 CCGCGG CCGC^GG
Sau3AI 22 GATC ^GATC
SfaNI 357 GATGC GCATC(5/9)
SfcI 49 CTGCAG C^T[GA][CT]AG
SmlI 388 CTCAAG C^T[CT][GA]AG
StyI 290 CCAAGG C^C[AT][AT]GG
Tsp45I 125 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BanII 9 GGGCTC G[GA]GC[CT]^C
294 GGGCTC
BlpI 296 GCTCAGC GC^T[ACGT]AGC
366 GCTGAGC
Bpu1102I 296 GCTCAGC GC^T[ACGT]AGC
366 GCTGAGC
BsaWI 24 TCCGGA [AT]^CCGG[AT]
561 ACCGGA
BspMI 473 ACCTGC ACCTGC(4/8)
247 GCAGGT
BstNI 383 CCAGG CC^[AT]GG
404 CCTGG
CelII 296 GCTCAGC GC^T[ACGT]AGC
366 GCTGAGC
DsaI 5 CCACGG C^C[GA][CT]GG
93 CCGCGG
EcoNI 74 CCTCCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
377 CCTTCTCCAGG
EcoRII 383 CCAGG ^CC[AT]GG
404 CCTGG
HphI 15 GGTGA GGTGA(8/7)
471 TCACC
MaeIII 125 GTGAC ^GT[ACGT]AC
461 GTTAC
MvaI 383 CCAGG CC^[AT]GG
404 CCTGG
TspRI 123 CAGTG CA[GC]TG[ACGT][ACGT]^
524 CAGTG
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BcnI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BsrI BssKI BstUI
Cac8I CfoI Cfr13I DdeI EaeI
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HpaII ItaI MnlI MspA1I
MspI MvnI MwoI NciI NlaIV
Sau96I ScrFI TseI