Restriction map for
pFN21AB7556
(Linear) (Six-base) MAPSORT of: KIBB7556.seq Check: 6991 from: 1 to: 813
KIBB7556.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 147 622 813
Size: 147 475 191
Fragments arranged by size:
475 191 147
ApaI G_GGCC'C
Cuts at: 0 182 255 813
Size: 182 73 558
Fragments arranged by size:
558 182 73
AvaI C'yCGr_G
Cuts at: 0 27 813
Size: 27 786
BanI G'GyrC_C
Cuts at: 0 111 562 813
Size: 111 451 251
Fragments arranged by size:
451 251 111
BanII G_rGCy'C
Cuts at: 0 34 182 255 813
Size: 34 148 73 558
Fragments arranged by size:
558 148 73 34
BbsI GAAGACnn'nnnn_
Cuts at: 0 693 813
Size: 693 120
BbvCI CC'TCA_GC
Cuts at: 0 735 813
Size: 735 78
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 494 528 813
Size: 494 34 285
Fragments arranged by size:
494 285 34
Bme1580I G_kGCm'C
Cuts at: 0 182 255 813
Size: 182 73 558
Fragments arranged by size:
558 182 73
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 813
Size: 251 562
Bpu10I CC'TnA_GC
Cuts at: 0 722 735 813
Size: 722 13 78
Fragments arranged by size:
722 78 13
BsaI GGTCTCn'nnnn_
Cuts at: 0 761 813
Size: 761 52
BsaWI w'CCGG_w
Cuts at: 0 371 813
Size: 371 442
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 136 166 813
Size: 136 30 647
Fragments arranged by size:
647 136 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 328 469 793 813
Size: 328 141 324 20
Fragments arranged by size:
328 324 141 20
BseYI C'CCAG_C
Cuts at: 0 92 181 691 712 813
Size: 92 89 510 21 101
Fragments arranged by size:
510 101 92 89 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 628 813
Size: 384 244 185
Fragments arranged by size:
384 244 185
BsiEI CG_ry'CG
Cuts at: 0 77 813
Size: 77 736
BsiHKAI G_wGCw'C
Cuts at: 0 34 813
Size: 34 779
Bsp1286I G_dGCh'C
Cuts at: 0 34 182 255 813
Size: 34 148 73 558
Fragments arranged by size:
558 148 73 34
BspHI T'CATG_A
Cuts at: 0 659 813
Size: 659 154
BsrBI CCG'CTC
Cuts at: 0 339 813
Size: 339 474
Bsu36I CC'TnA_GG
Cuts at: 0 635 813
Size: 635 178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 727 813
Size: 727 86
BtsI GCAGTG_nn'
Cuts at: 0 157 640 813
Size: 157 483 173
Fragments arranged by size:
483 173 157
DraIII CAC_nnn'GTG
Cuts at: 0 111 813
Size: 111 702
EaeI y'GGCC_r
Cuts at: 0 74 235 356 813
Size: 74 161 121 457
Fragments arranged by size:
457 161 121 74
EagI C'GGCC_G
Cuts at: 0 74 813
Size: 74 739
EarI CTCTTCn'nnn_
Cuts at: 0 305 813
Size: 305 508
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 418 813
Size: 418 395
EcoICRI GAG'CTC
Cuts at: 0 32 813
Size: 32 781
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 813
Size: 251 562
EcoO109I rG'GnC_Cy
Cuts at: 0 178 252 619 813
Size: 178 74 367 194
Fragments arranged by size:
367 194 178 74
HaeII r_GCGC'y
Cuts at: 0 321 480 813
Size: 321 159 333
Fragments arranged by size:
333 321 159
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 123 155 195 227 415 447 760 792
Size: 123 32 40 32 188 32 313 32
Cuts at: 792 813
Size: 21
Fragments arranged by size:
313 188 123 40 32 32 32 32
21
MslI CAynn'nnrTG
Cuts at: 0 658 813
Size: 658 155
MspA1I CmG'CkG
Cuts at: 0 764 813
Size: 764 49
PasI CC'CwG_GG
Cuts at: 0 255 616 813
Size: 255 361 197
Fragments arranged by size:
361 255 197
PfoI T'CCnGG_A
Cuts at: 0 34 521 813
Size: 34 487 292
Fragments arranged by size:
487 292 34
PspOMI G'GGCC_C
Cuts at: 0 178 251 813
Size: 178 73 562
Fragments arranged by size:
562 178 73
PstI C_TGCA'G
Cuts at: 0 152 401 443 464 813
Size: 152 249 42 21 349
Fragments arranged by size:
349 249 152 42 21
SacI G_AGCT'C
Cuts at: 0 34 813
Size: 34 779
SbfI CC_TGCA'GG
Cuts at: 0 401 813
Size: 401 412
SexAI A'CCwGG_T
Cuts at: 0 107 813
Size: 107 706
SfcI C'TryA_G
Cuts at: 0 148 397 439 460 813
Size: 148 249 42 21 353
Fragments arranged by size:
353 249 148 42 21
StuI AGG'CCT
Cuts at: 0 198 285 813
Size: 198 87 528
Fragments arranged by size:
528 198 87
StyI C'CwwG_G
Cuts at: 0 533 786 813
Size: 533 253 27
Fragments arranged by size:
533 253 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 403 813
Size: 403 410
TatI w'GTAC_w
Cuts at: 0 215 813
Size: 215 598
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 136 168 813
Size: 136 32 645
Fragments arranged by size:
645 136 32
Enzymes that do cut:
AlwNI ApaI AvaI BanI BanII BbsI BbvCI BcgI
Bme1580I BpmI Bpu10I BsaI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BsrBI Bsu36I BtgZI
BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I HaeII Hin4I MslI MspA1I PasI PfoI PspOMI
PstI SacI SbfI SexAI SfcI StuI StyI TaqII
TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI BpuEI BsaAI BsaBI BsaHI BsiWI BsmI BsmBI
BspEI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI ClaI CspCI
DraI DrdI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SapI ScaI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI