Restriction map for
pFN21AB7549
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AvaII AviII AvrII
BamHI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
CfoI Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HphI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MroI MspA1I MunI
MvnI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI Sau96I SbfI ScaI SexAI
SfiI SfoI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tsp45I Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AflIII 11 ACACGT A^C[GA][CT]GT
AhdI 121 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 527 AGCT AG^CT
AlwI 465 GATCC GGATC(4/5)
AlwNI 127 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 121 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 327 GAGCAC G[AT]GC[AT]^C
AvaI 335 CCCGGG C^[CT]CG[GA]G
BalI 93 TGGCCA TGG^CCA
BanI 433 GGCACC G^G[CT][GA]CC
BbsI 198 GAAGAC GAAGAC(2/6)
BglI 363 GCCCATGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 327 GAGCAC G[AGT]GC[ACT]^C
BpuAI 198 GAAGAC GAAGAC(2/6)
BsaI 448 GAGACC GGTCTC(1/5)
BsiHKAI 327 GAGCAC G[AT]GC[AT]^C
BsiYI 126 CCAGTCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 126 CCAGTCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 448 GAGAC GTCTC(1/5)
BsoBI 335 CCCGGG C^[CT]CG[GA]G
Bsp1286I 327 GAGCAC G[AGT]GC[ACT]^C
BspHI 230 TCATGA T^CATGA
BspMI 273 GCAGGT ACCTGC(4/8)
BsrBI 21 GAGCGG CCGCTC(-3/-3)
BsrFI 531 GCCGGC [GA]^CCGG[CT]
BstBI 265 TTCGAA TT^CGAA
BstNI 132 CCTGG CC^[AT]GG
BstXI 365 CCATGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 464 AGATCC [GA]^GATC[CT]
Cfr10I 531 GCCGGC [GA]^CCGG[CT]
DpnII 465 GATC ^GATC
EaeI 93 TGGCCA [CT]^GGCC[GA]
Eam1105I 121 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII 132 CCTGG ^CC[AT]GG
HaeIII 94 GGCC GG^CC
MaeII 13 ACGT A^CGT
MaeIII 317 GTAAC ^GT[ACGT]AC
MboI 465 GATC ^GATC
MflI 464 AGATCC [GA]^GATC[CT]
MluNI 93 TGGCCA TGG^CCA
MscI 93 TGGCCA TGG^CCA
MseI 212 TTAA T^TAA
MslI 360 CATGCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 132 CCTGG CC^[AT]GG
NaeI 531 GCCGGC GCC^GGC
NdeII 465 GATC ^GATC
NgoMIV 531 GCCGGC G^CCGGC
NspI 178 ACATGC [GA]CATG^[CT]
NspV 265 TTCGAA TT^CGAA
PflMI 126 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 509 GACTC GAGTC(4/5)
PstI 185 CTGCAG CTGCA^G
RcaI 230 TCATGA T^CATGA
Sau3AI 465 GATC ^GATC
SfuI 265 TTCGAA TT^CGAA
SmaI 335 CCCGGG CCC^GGG
Tru9I 212 TTAA T^TAA
TspRI 111 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 126 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 194 CCAAGAAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 464 AGATCC [GA]^GATC[CT]
XmaCI 335 CCCGGG C^CCGGG
XmaI 335 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AciI 23 GCGG CCGC(-3/-1)
369 GCGG
AfaI 85 GTAC GT^AC
277 GTAC
BbvI 53 GCAGC GCAGC(8/12)
528 GCTGC
BcnI 335 CCCGG CC^[GC]GG
336 CCGGG
BsaJI 131 CCCTGG C^C[ACGT][ACGT]GG
335 CCCGGG
BsrI 126 CCAGT ACTGG(1/-1)
556 CCAGT
HapII 336 CCGG C^CGG
532 CCGG
HgaI 201 GACGC GACGC(5/10)
297 GCGTC
HpaII 336 CCGG C^CGG
532 CCGG
MspI 336 CCGG C^CGG
532 CCGG
MwoI 47 GCATCGGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
363 GCCCATGCGGC
NciI 335 CCCGG CC^[GC]GG
336 CCGGG
NlaIV 386 GGAACC GG[ACGT]^[ACGT]CC
433 GGCACC
RsaI 85 GTAC GT^AC
277 GTAC
SfaNI 47 GCATC GCATC(5/9)
482 GCATC
SfcI 42 CTACAG C^T[GA][CT]AG
185 CTGCAG
TaqI 266 TCGA T^CGA
446 TCGA
TfiI 107 GAATC G^A[AT]TC
393 GATTC
TseI 53 GCAGC G^C[AT]GC
528 GCTGC
TthHB8I 266 TCGA T^CGA
446 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
BfaI BsgI BsmFI BssKI BstF5I
Cac8I DdeI Fnu4HI FokI HinfI
ItaI MaeI MboII MnlI NlaIII
ScrFI Tsp509I