Restriction map for pFN21AB7549
 (Circular) (Six-base) MAPSORT of: pFN21AB7549.seq  Check: 1266  from: 1  to: 5284



pFN21AB7549  5284 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:39  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3094     278 
   Size:         53      83     186     936    1558    2468

  Fragments arranged by size: 

                2468    1558     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2627    1937 
   Size:        690    4594
 
Acc65I G'GTAC_C

Cuts at:   2605    2605 
   Size:       5284
 
AclI AA'CG_TT

Cuts at:   1563    3411    3784    1563 
   Size:       1848     373    3063

  Fragments arranged by size: 

                3063    1848     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2273    3347    4731    4999    1632 
   Size:        641    1074    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1074     641     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5087
 
AflIII A'CryG_T

Cuts at:   1203    2016    4183    5160    1203 
   Size:        813    2167     977    1327

  Fragments arranged by size: 

                2167    1327     977     813
 
AgeI A'CCGG_T

Cuts at:   4115    4115 
   Size:       5284
 
AhdI GACnn_n'nnGTC

Cuts at:   2131    4013    2131 
   Size:       1882    3402
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5284
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2084    2490    1184 
   Size:        900     406    3978

  Fragments arranged by size: 

                3978     900     406
 
AlwNI CAG_nnn'CTG

Cuts at:   2137    3027    4599    2137 
   Size:        890    1572    2822

  Fragments arranged by size: 

                2822    1572     890
 
ApaLI G'TGCA_C

Cuts at:   3341    4497    3341 
   Size:       1156    4128
 
ApoI r'AATT_y

Cuts at:   1493    2593    2819    2971    5080    1493 
   Size:       1100     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1100     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3838     160 
   Size:        872    2806    1606

  Fragments arranged by size: 

                2806    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5284
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2340    2609    1870 
   Size:         73     397     269    4545

  Fragments arranged by size: 

                4545     397     269      73
 
BamHI G'GATC_C

Cuts at:   2614    2981    2614 
   Size:        367    4917
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2438    2605    4060     618 
   Size:        325     217     627     651     167    1455    1842

  Fragments arranged by size: 

                1842    1455     651     627     325     217     167
 
BanII G_rGCy'C

Cuts at:   1391    2603    1391 
   Size:       1212    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2210     928 
   Size:        971     311    4002

  Fragments arranged by size: 

                4002     971     311
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3475    3509    1903 
   Size:         34    1538      34    3678

  Fragments arranged by size: 

                3678    1538      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3176    4386    3176 
   Size:       1210    4074
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5284
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2374    3895     136 
   Size:        107     122      71     631     605     702    1521    1525

  Fragments arranged by size: 

                1525    1521     702     631     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5279    5279 
   Size:       5284
 
BlpI GC'TnA_GC

Cuts at:   2701    2701 
   Size:       5284
 
Bme1580I G_kGCm'C

Cuts at:   1790    3345    4501    1790 
   Size:       1555    1156    2573

  Fragments arranged by size: 

                2573    1555    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5284
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2555    3015    3973 
   Size:        416      53      83     399      24    1138     460     958

Cuts at:   3973     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1138     958     460     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5284
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3944    1239 
   Size:       2705    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3409    4274    4572    3409 
   Size:        865     298    4121

  Fragments arranged by size: 

                4121     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2447    3947     882 
   Size:        617     948    1500    2219

  Fragments arranged by size: 

                2219    1500     948     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3075     493 
   Size:        711    1871    2702

  Fragments arranged by size: 

                2702    1871     711
 
BsaBI GATnn'nnATC

Cuts at:   5278    5278 
   Size:       5284
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3091    3473    4108     275 
   Size:         53      83     186     936    1558     382     635    1451

  Fragments arranged by size: 

                1558    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3717    4115    4389    4536    4947    3717 
   Size:        398     274     147     411    4054

  Fragments arranged by size: 

                4054     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2541    2571    1178 
   Size:         30     165      30    1138      30    3891

  Fragments arranged by size: 

                3891    1138     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5284
 
BseYI C'CCAG_C

Cuts at:   2209    4487    4983    2209 
   Size:       2278     496    2510

  Fragments arranged by size: 

                2510    2278     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2297    2344    2486    1163 
   Size:        573     561      47     142    3961

  Fragments arranged by size: 

                3961     573     561     142      47
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2734    3495    4523    1540 
   Size:        275     186     733     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     733     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2336    3345    3430    4501    2336 
   Size:       1009      85    1071    3119

  Fragments arranged by size: 

                3119    1071    1009      85
 
BsmI GAATG_Cn'

Cuts at:   2795    2888    2795 
   Size:         93    5191
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2336    2603    3345    3430    4501    1391 
   Size:        399     546     267     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     546     399     267      85
 
BspEI T'CCGG_A

Cuts at:   4947    4947 
   Size:       5284
 
BspHI T'CATG_A

Cuts at:   2235    3173    4903    2235 
   Size:        938    1730    2616

  Fragments arranged by size: 

                2616    1730     938
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2269    2639     844 
   Size:       1425     370    3489

  Fragments arranged by size: 

                3489    1425     370
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2028    3171    1394 
   Size:        429     205    1143    3507

  Fragments arranged by size: 

                3507    1143     429     205
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3779    3953      65 
   Size:       1183    2531     174    1396

  Fragments arranged by size: 

                2531    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2536    3928    4115    1700 
   Size:        110     726    1392     187    2869

  Fragments arranged by size: 

                2869    1392     726     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5284
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5284
 
BssSI C'ACGA_G

Cuts at:   3344    4356    3344 
   Size:       1012    4272
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5284
 
BstBI TT'CG_AA

Cuts at:   2271    4088    2271 
   Size:       1817    3467
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5284
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2377    2377 
   Size:       5284
 
BstYI r'GATC_y

Cuts at:   1972    2469    2614    2981    3366    3383    4835    4921    4933 
   Size:        497     145     367     385      17    1452      86      12

Cuts at:   4933    5279    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     497     385     367     346     145      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    4732
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4545

  Fragments arranged by size: 

                4545     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2800    3594    3614    2800 
   Size:        794      20    4470

  Fragments arranged by size: 

                4470     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5284
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5249
 
DraI TTT'AAA

Cuts at:   2588    2588 
   Size:       5284
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4291     809 
   Size:        507    2975    1802

  Fragments arranged by size: 

                2975    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2098    2731    3620 
   Size:         54    1425      50     275      18     268     633     889

Cuts at:   3620    5249       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     633     275     268      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2731    1537 
   Size:        275     919    4090

  Fragments arranged by size: 

                4090     919     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3214    1353 
   Size:       1861    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3871    4245    4391     387 
   Size:       1064     138    2282     374     146    1280

  Fragments arranged by size: 

                2282    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2273    3347    3944    4731    4999    1239 
   Size:        393     641    1074     597     787     268    1524

  Fragments arranged by size: 

                1524    1074     787     641     597     393     268
 
EcoRI G'AATT_C

Cuts at:   2593    2971    2593 
   Size:        378    4906
 
FspI TGC'GCA

Cuts at:   3790    3790 
   Size:       5284
 
HaeII r_GCGC'y

Cuts at:   1678    4431    1678 
   Size:       2753    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2117    2149    2454    2486    3925 
   Size:         32     135      32     545      32     305      32    1439

Cuts at:   3925    3957    3999    4031    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1439     545     305     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2628    2874     669 
   Size:        988     281     690     246    3079

  Fragments arranged by size: 

                3079     988     690     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5284
 
HpaI GTT'AAC

Cuts at:   2874    2874 
   Size:       5284
 
KpnI G_GTAC'C

Cuts at:   2609    2609 
   Size:       5284
 
MfeI C'AATT_G

Cuts at:   2883    2883 
   Size:       5284
 
MluI A'CGCG_T

Cuts at:   5160    5160 
   Size:       5284
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2515    4398    4582    1305 
   Size:       1210    1883     184    2007

  Fragments arranged by size: 

                2007    1883    1210     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2100      10 
   Size:         54    1425     343     268    3194

  Fragments arranged by size: 

                3194    1425     343     268      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2369    3242    3601    3760     518 
   Size:        648    1203     873     359     159    2042

  Fragments arranged by size: 

                2042    1203     873     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2700    3377    4525    4770    1606 
   Size:       1094     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1094     677     245
 
NaeI GCC'GGC

Cuts at:   2538    2538 
   Size:       5284
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4732
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5284
 
NgoMIV G'CCGG_C

Cuts at:   2536    2536 
   Size:       5284
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5284
 
NotI GC'GGCC_GC

Cuts at:   2731    2731 
   Size:       5284
 
NspI r_CATG'y

Cuts at:   1132    1229    2187    2642    4187    1132 
   Size:         97     958     455    1545    2229

  Fragments arranged by size: 

                2229    1545     958     455      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5284
 
PciI A'CATG_T

Cuts at:   4183    4183 
   Size:       5284
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2137    1108 
   Size:       1029    4255
 
PmeI GTTT'AAAC

Cuts at:   2588    2588 
   Size:       5284
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3353    3385    4866    4898    3353 
   Size:         32    1481      32    3739

  Fragments arranged by size: 

                3739    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5284
 
PsiI TTA'TAA

Cuts at:   2854    2854 
   Size:       5284
 
PstI C_TGCA'G

Cuts at:    830    2194    2636     830 
   Size:       1364     442    3478

  Fragments arranged by size: 

                3478    1364     442
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5284
 
SalI G'TCGA_C

Cuts at:   1936    2626    1936 
   Size:        690    4594
 
SbfI CC_TGCA'GG

Cuts at:   2636    2636 
   Size:       5284
 
ScaI AGT'ACT

Cuts at:   3532    5244    3532 
   Size:       1712    3572
 
SfcI C'TryA_G

Cuts at:    826    1045    2047    2190    2632    3767    4448    4639     826 
   Size:        219    1002     143     442    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1002     681     442     219     191     143
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5284
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5284
 
SmaI CCC'GGG

Cuts at:   1872    2342    2611    1872 
   Size:        470     269    4545

  Fragments arranged by size: 

                4545     470     269
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3388    4289    4551     820 
   Size:        197     926    1445     901     262    1553

  Fragments arranged by size: 

                1553    1445     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3075     493 
   Size:       2582    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5284
 
SphI G_CATG'C

Cuts at:   1132    2642    1132 
   Size:       1510    3774
 
SspI AAT'ATT

Cuts at:      5      52    3208       5 
   Size:         47    3156    2081

  Fragments arranged by size: 

                3156    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5284
 
StyI C'CwwG_G

Cuts at:    513    1065    2723     513 
   Size:        552    1658    3074

  Fragments arranged by size: 

                3074    1658     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3311    3328    3481    3666    4111     325 
   Size:       1255    1731      17     153     185     445    1498

  Fragments arranged by size: 

                1731    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3530    5242      96 
   Size:        274      80      33      51    1443    1553    1712     138

  Fragments arranged by size: 

                1712    1553    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5244    5276    5244 
   Size:         32    5252
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5284
 
XbaI T'CTAG_A

Cuts at:   2620    2620 
   Size:       5284
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2206    2206 
   Size:       5284
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5284
 
XmaI C'CCGG_G

Cuts at:   1870    2340    2609    1870 
   Size:        470     269    4545

  Fragments arranged by size: 

                4545     470     269
 
XmnI GAAnn'nnTTC

Cuts at:   3413    3413 
   Size:       5284
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3092     276 
   Size:         53      83     186     936    1558    2468

  Fragments arranged by size: 

                2468    1558     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NcoI
     NdeI   NgoMIV     NheI     NotI     NspI     PasI     PciI    PflMI
     PmeI     PpiI    PshAI     PsiI     PstI     PvuI     SalI     SbfI
     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI
     KasI     NarI     NruI     NsiI     PacI     PfoI     PmlI    PpuMI
   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI    SacII    SanDI
     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
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