Restriction map for
pFN21AB7542
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AclI AfaI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BbrPI BbsI BciVI BcnI BfrI
BglI BglII BlnI BpuAI BsaBI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FseI FspI
HgaI HincII HindII HindIII HpaI
HphI KpnI MamI MfeI MluI
MluNI MscI MunI NaeI NciI
NcoI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccIII 173 TCCGGA T^CCGGA
AcsI 145 GAATTT [GA]^AATT[CT]
AcyI 25 GGCGCC G[GA]^CG[CT]C
ApoI 145 GAATTT [GA]^AATT[CT]
AspHI 523 GAGCTC G[AT]GC[AT]^C
AvaII 90 GGACC G^G[AT]CC
BamHI 231 GGATCC G^GATCC
BanI 25 GGCGCC G^G[CT][GA]CC
BanII 523 GAGCTC G[GA]GC[CT]^C
BbeI 25 GGCGCC GGCGC^C
BcgI 464 GCACTGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 431 TGATCA T^GATCA
BlpI 520 GCTGAGC GC^T[ACGT]AGC
BmyI 523 GAGCTC G[AGT]GC[ACT]^C
BpmI 139 CTGGAG CTGGAG(16/14)
Bpu1102I 520 GCTGAGC GC^T[ACGT]AGC
BsaAI 574 TACGTG [CT]AC^GT[GA]
BsaHI 25 GGCGCC G[GA]^CG[CT]C
BsaI 613 GAGACC GGTCTC(1/5)
BsaWI 173 TCCGGA [AT]^CCGG[AT]
BseAI 173 TCCGGA T^CCGGA
BseRI 751 GAGGAG GAGGAG(10/8)
BsgI 196 GTGCAG GTGCAG(16/14)
BsiHKAI 523 GAGCTC G[AT]GC[AT]^C
BsmAI 613 GAGAC GTCTC(1/5)
Bsp1286I 523 GAGCTC G[AGT]GC[ACT]^C
BspEI 173 TCCGGA T^CCGGA
BspMI 644 ACCTGC ACCTGC(4/8)
BstXI 221 CCATTGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 520 GCTGAGC GC^T[ACGT]AGC
DraII 680 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 538 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 715 CTCTTC CTCTTC(1/4)
EcoNI 645 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 680 AGGCCCC [GA]G^G[ACGT]CC[CT]
FauI 11 GCGGG CCCGC(4/6)
FbaI 431 TGATCA T^GATCA
HapII 174 CCGG C^CGG
Hin1I 25 GGCGCC G[GA]^CG[CT]C
HinfI 180 GAATC G^A[ACGT]TC
HpaII 174 CCGG C^CGG
KasI 25 GGCGCC G^GCGCC
Ksp632I 715 CTCTTC CTCTTC(1/4)
KspI 9 CCGCGG CCGC^GG
MaeII 575 ACGT A^CGT
MaeIII 577 GTGAC ^GT[ACGT]AC
MroI 173 TCCGGA T^CCGGA
MseI 605 TTAA T^TAA
MspI 174 CCGG C^CGG
NarI 25 GGCGCC GG^CGCC
NheI 96 GCTAGC G^CTAGC
PstI 550 CTGCAG CTGCA^G
SacI 523 GAGCTC GAGCT^C
SacII 9 CCGCGG CCGC^GG
SfcI 550 CTGCAG C^T[GA][CT]AG
SfoI 25 GGCGCC GGC^GCC
TaqI 314 TCGA T^CGA
TfiI 180 GAATC G^A[AT]TC
Tru9I 605 TTAA T^TAA
Tsp45I 577 GTGAC ^GT[GC]AC
TthHB8I 314 TCGA T^CGA
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 10 CGCG CG^CG
626 CGCG
AflIII 122 ACATGT A^C[GA][CT]GT
209 ACATGT
BbvCI 85 GCTGAGG CCTCAGC(-5/-2)
748 GCTGAGG
BfaI 97 CTAG C^TAG
298 CTAG
BsmFI 73 GGGAC GGGAC(10/14)
281 GGGAC
BspLU11I 122 ACATGT A^CATGT
209 ACATGT
BstF5I 480 CATCC GGATG(2/0)
411 GGATG
BstUI 10 CGCG CG^CG
626 CGCG
BstYI 170 AGATCC [GA]^GATC[CT]
231 GGATCC
DsaI 9 CCGCGG C^C[GA][CT]GG
542 CCGTGG
FokI 480 CATCC GGATG(9/13)
411 GGATG
HaeII 25 GGCGCC [GA]GCGC^[CT]
327 GGCGCT
MaeI 97 CTAG C^TAG
298 CTAG
MflI 170 AGATCC [GA]^GATC[CT]
231 GGATCC
MvnI 10 CGCG CG^CG
626 CGCG
NlaIII 123 CATG CATG^
210 CATG
NspI 122 ACATGT [GA]CATG^[CT]
209 ACATGT
Tsp509I 146 AATT ^AATT
309 AATT
XhoII 170 AGATCC [GA]^GATC[CT]
231 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI AlwI BbvI BsaJI
BsiYI BslI BsrI BssKI BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII Fnu4HI HaeIII HhaI HinP1I
ItaI MboI MboII MnlI MslI
MspA1I MvaI MwoI NdeII NlaIV
PvuII Sau3AI Sau96I ScrFI SfaNI
TseI TspRI