(Circular) (Six-base) MAPSORT of: pFN21AB7540.seq Check: 78 from: 1 to: 5479 pFN21AB7540 5479 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3289 278 Size: 53 83 186 936 1753 2468 Fragments arranged by size: 2468 1753 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2822 1937 Size: 885 4594 Acc65I G'GTAC_C Cuts at: 2800 2800 Size: 5479 AclI AA'CG_TT Cuts at: 1563 3606 3979 1563 Size: 2043 373 3063 Fragments arranged by size: 3063 2043 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3542 4926 5194 1632 Size: 1910 1384 268 1917 Fragments arranged by size: 1917 1910 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5282 AflIII A'CryG_T Cuts at: 1203 4378 5355 1203 Size: 3175 977 1327 Fragments arranged by size: 3175 1327 977 AgeI A'CCGG_T Cuts at: 4310 4310 Size: 5479 AhdI GACnn_n'nnGTC Cuts at: 4208 4208 Size: 5479 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5479 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5479 AlwNI CAG_nnn'CTG Cuts at: 2350 2551 2587 2677 3222 4794 2350 Size: 201 36 90 545 1572 3035 Fragments arranged by size: 3035 1572 545 201 90 36 ApaI G_GGCC'C Cuts at: 2278 2580 2739 2278 Size: 302 159 5018 Fragments arranged by size: 5018 302 159 ApaLI G'TGCA_C Cuts at: 3536 4692 3536 Size: 1156 4323 ApoI r'AATT_y Cuts at: 1493 2788 3014 3166 5275 1493 Size: 1295 226 152 2109 1697 Fragments arranged by size: 2109 1697 1295 226 152 AseI AT'TA_AT Cuts at: 160 1032 4033 160 Size: 872 3001 1606 Fragments arranged by size: 3001 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5479 AvaI C'yCGr_G Cuts at: 1870 1943 2128 2146 2166 2482 2739 2804 1870 Size: 73 185 18 20 316 257 65 4545 Fragments arranged by size: 4545 316 257 185 73 65 20 18 BamHI G'GATC_C Cuts at: 2809 3176 2809 Size: 367 5112 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2162 2563 2800 4255 618 Size: 325 217 627 375 401 237 1455 1842 Fragments arranged by size: 1842 1455 627 401 375 325 237 217 BanII G_rGCy'C Cuts at: 1391 2175 2278 2357 2580 2739 2798 1391 Size: 784 103 79 223 159 59 4072 Fragments arranged by size: 4072 784 223 159 103 79 59 BbeI G_GCGC'C Cuts at: 2166 2166 Size: 5479 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2323 2514 928 Size: 971 424 191 3893 Fragments arranged by size: 3893 971 424 191 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3670 3704 1903 Size: 34 50 34 1649 34 3678 Fragments arranged by size: 3678 1649 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3371 4581 3371 Size: 1210 4269 BclI T'GATC_A Cuts at: 1548 1548 Size: 5479 BfrBI ATG'CAT Cuts at: 2774 2774 Size: 5479 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2142 4090 136 Size: 107 122 71 631 605 470 1948 1525 Fragments arranged by size: 1948 1525 631 605 470 122 107 71 BglII A'GATC_T Cuts at: 5474 5474 Size: 5479 BlpI GC'TnA_GC Cuts at: 2896 2896 Size: 5479 Bme1580I G_kGCm'C Cuts at: 1790 2278 2558 2566 2580 2739 3540 4696 1790 Size: 488 280 8 14 159 801 1156 2573 Fragments arranged by size: 2573 1156 801 488 280 159 14 8 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5479 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3210 4168 442 Size: 416 53 83 399 24 1793 958 1753 Fragments arranged by size: 1793 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5479 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2032 2381 4139 1239 Size: 793 349 1758 2579 Fragments arranged by size: 2579 1758 793 349 Bpu10I CC'TnA_GC Cuts at: 2089 2351 2089 Size: 262 5217 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3604 4469 4767 3604 Size: 865 298 4316 Fragments arranged by size: 4316 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2493 2621 4142 882 Size: 617 994 128 1521 2219 Fragments arranged by size: 2219 1521 994 617 128 BsaAI yAC'GTr Cuts at: 493 1204 3270 493 Size: 711 2066 2702 Fragments arranged by size: 2702 2066 711 BsaBI GATnn'nnATC Cuts at: 5473 5473 Size: 5479 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2163 2328 2681 3286 Size: 53 83 186 936 630 165 353 605 Cuts at: 3286 3668 4303 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 635 630 605 382 353 186 165 83 53 BsaWI w'CCGG_w Cuts at: 3912 4310 4584 4731 5142 3912 Size: 398 274 147 411 4249 Fragments arranged by size: 4249 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2057 2087 1178 Size: 30 165 30 654 30 4570 Fragments arranged by size: 4570 654 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2005 2008 1186 Size: 819 3 4657 Fragments arranged by size: 4657 819 3 BseYI C'CCAG_C Cuts at: 2463 2503 2713 4682 5178 2463 Size: 40 210 1969 496 2764 Fragments arranged by size: 2764 1969 496 210 40 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2453 1163 Size: 573 717 4189 Fragments arranged by size: 4189 717 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2929 3690 4718 1540 Size: 275 186 928 761 1028 2301 Fragments arranged by size: 2301 1028 928 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3540 3625 4696 3540 Size: 85 1071 4323 Fragments arranged by size: 4323 1071 85 BsmI GAATG_Cn' Cuts at: 2990 3083 2990 Size: 93 5386 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2175 2278 2357 2558 2566 2580 2739 Size: 399 385 103 79 201 8 14 159 Cuts at: 2739 2798 3540 3625 4696 1391 Size: 59 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 385 201 159 103 85 79 59 14 8 BspEI T'CCGG_A Cuts at: 5142 5142 Size: 5479 BspHI T'CATG_A Cuts at: 3368 5098 3368 Size: 1730 3749 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2299 2834 844 Size: 1455 535 3489 Fragments arranged by size: 3489 1455 535 BsrBI CCG'CTC Cuts at: 1394 1823 2365 3366 1394 Size: 429 542 1001 3507 Fragments arranged by size: 3507 1001 542 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3974 4148 65 Size: 1183 2726 174 1396 Fragments arranged by size: 2726 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2703 4123 4310 1700 Size: 110 893 1420 187 2869 Fragments arranged by size: 2869 1420 893 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5479 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5479 BssSI C'ACGA_G Cuts at: 2028 3539 4551 2028 Size: 1511 1012 2956 Fragments arranged by size: 2956 1511 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5479 BstBI TT'CG_AA Cuts at: 4283 4283 Size: 5479 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5479 BstYI r'GATC_y Cuts at: 1972 2809 3176 3561 3578 5030 5116 5128 5474 Size: 837 367 385 17 1452 86 12 346 Cuts at: 5474 1972 Size: 1977 Fragments arranged by size: 1977 1452 837 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2178 2178 Size: 5479 BtgI C'CryG_G Cuts at: 513 1065 2061 2238 2301 2730 513 Size: 552 996 177 63 429 3262 Fragments arranged by size: 3262 996 552 429 177 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4740 Fragments arranged by size: 4740 683 56 BtsI GCAGTG_nn' Cuts at: 2995 3789 3809 2995 Size: 794 20 4665 Fragments arranged by size: 4665 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5479 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5444 DraI TTT'AAA Cuts at: 2783 2783 Size: 5479 DraIII CAC_nnn'GTG Cuts at: 2030 2030 Size: 5479 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4486 809 Size: 507 3170 1802 Fragments arranged by size: 3170 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2241 2376 2926 Size: 54 1425 50 275 18 411 135 550 Cuts at: 2926 3815 5444 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 550 411 275 135 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2926 1537 Size: 275 1114 4090 Fragments arranged by size: 4090 1114 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2363 3409 1353 Size: 1010 1046 3423 Fragments arranged by size: 3423 1046 1010 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4066 4440 4586 387 Size: 1064 138 2477 374 146 1280 Fragments arranged by size: 2477 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2032 2381 3542 4139 4926 5194 1239 Size: 393 400 349 1161 597 787 268 1524 Fragments arranged by size: 1524 1161 787 597 400 393 349 268 EcoNI CCTnn'n_nnAGG Cuts at: 2697 2697 Size: 5479 EcoO109I rG'GnC_Cy Cuts at: 2208 2275 2458 2577 2638 2735 2736 2743 2208 Size: 67 183 119 61 97 1 7 4944 Fragments arranged by size: 4944 183 119 97 67 61 7 1 EcoRI G'AATT_C Cuts at: 2788 3166 2788 Size: 378 5101 FseI GG_CCGG'CC Cuts at: 2707 2707 Size: 5479 FspI TGC'GCA Cuts at: 3985 3985 Size: 5479 HaeII r_GCGC'y Cuts at: 1678 2166 4626 1678 Size: 488 2460 2531 Fragments arranged by size: 2531 2460 488 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2057 2089 4120 4152 4194 Size: 32 135 32 485 32 2031 32 42 Cuts at: 4194 4226 1373 Size: 32 2626 Fragments arranged by size: 2626 2031 485 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2823 3069 669 Size: 988 281 885 246 3079 Fragments arranged by size: 3079 988 885 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5479 HpaI GTT'AAC Cuts at: 3069 3069 Size: 5479 KasI G'GCGC_C Cuts at: 2162 2162 Size: 5479 KpnI G_GTAC'C Cuts at: 2804 2804 Size: 5479 MfeI C'AATT_G Cuts at: 3078 3078 Size: 5479 MluI A'CGCG_T Cuts at: 5355 5355 Size: 5479 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4593 4777 1305 Size: 3288 184 2007 Fragments arranged by size: 3288 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2378 10 Size: 54 1425 343 546 3111 Fragments arranged by size: 3111 1425 546 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3437 3796 3955 518 Size: 648 2271 359 159 2042 Fragments arranged by size: 2271 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2085 2467 2548 2895 3572 4720 4965 1606 Size: 479 382 81 347 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 479 382 347 245 81 NaeI GCC'GGC Cuts at: 2705 2705 Size: 5479 NarI GG'CG_CC Cuts at: 2163 2163 Size: 5479 NcoI C'CATG_G Cuts at: 513 1065 2238 2301 513 Size: 552 1173 63 3691 Fragments arranged by size: 3691 1173 552 63 NdeI CA'TA_TG Cuts at: 387 387 Size: 5479 NgoMIV G'CCGG_C Cuts at: 2703 2703 Size: 5479 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5479 NotI GC'GGCC_GC Cuts at: 2926 2926 Size: 5479 NsiI A_TGCA'T Cuts at: 2776 2776 Size: 5479 NspI r_CATG'y Cuts at: 1132 1229 2837 4382 1132 Size: 97 1608 1545 2229 Fragments arranged by size: 2229 1608 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5479 PciI A'CATG_T Cuts at: 4378 4378 Size: 5479 PflMI CCAn_nnn'nTGG Cuts at: 1108 2030 1108 Size: 922 4557 PmeI GTTT'AAAC Cuts at: 2783 2783 Size: 5479 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3548 3580 5061 5093 3548 Size: 32 1481 32 3934 Fragments arranged by size: 3934 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2208 2458 2208 Size: 250 5229 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5479 PsiI TTA'TAA Cuts at: 3049 3049 Size: 5479 PspOMI G'GGCC_C Cuts at: 2274 2576 2735 2274 Size: 302 159 5018 Fragments arranged by size: 5018 302 159 PstI C_TGCA'G Cuts at: 830 2547 2831 830 Size: 1717 284 3478 Fragments arranged by size: 3478 1717 284 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5479 PvuII CAG'CTG Cuts at: 2085 2467 2548 2085 Size: 382 81 5016 Fragments arranged by size: 5016 382 81 SalI G'TCGA_C Cuts at: 1936 2821 1936 Size: 885 4594 SbfI CC_TGCA'GG Cuts at: 2831 2831 Size: 5479 ScaI AGT'ACT Cuts at: 3727 5439 3727 Size: 1712 3767 SexAI A'CCwGG_T Cuts at: 2414 2414 Size: 5479 SfcI C'TryA_G Cuts at: 826 1045 2543 2827 3962 4643 4834 826 Size: 219 1498 284 1135 681 191 1471 Fragments arranged by size: 1498 1471 1135 681 284 219 191 SfoI GGC'GCC Cuts at: 2164 2164 Size: 5479 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5479 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5479 SmaI CCC'GGG Cuts at: 1872 2130 2148 2168 2741 2806 1872 Size: 258 18 20 573 65 4545 Fragments arranged by size: 4545 573 258 65 20 18 SmlI C'TyrA_G Cuts at: 820 1017 1943 3583 4484 4746 820 Size: 197 926 1640 901 262 1553 Fragments arranged by size: 1640 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3270 493 Size: 2777 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5479 SphI G_CATG'C Cuts at: 1132 2837 1132 Size: 1705 3774 SrfI GCCC'GGGC Cuts at: 2148 2148 Size: 5479 SspI AAT'ATT Cuts at: 5 52 3403 5 Size: 47 3351 2081 Fragments arranged by size: 3351 2081 47 StuI AGG'CCT Cuts at: 1517 2113 1517 Size: 596 4883 StyI C'CwwG_G Cuts at: 513 1065 2238 2301 2319 2558 2918 513 Size: 552 1173 63 18 239 360 3074 Fragments arranged by size: 3074 1173 552 360 239 63 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3506 3523 3676 3861 4306 325 Size: 1255 1926 17 153 185 445 1498 Fragments arranged by size: 1926 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3725 5437 96 Size: 274 80 33 51 1443 1748 1712 138 Fragments arranged by size: 1748 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5439 5471 5439 Size: 32 5447 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5479 XbaI T'CTAG_A Cuts at: 2815 2815 Size: 5479 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5479 XmaI C'CCGG_G Cuts at: 1870 2128 2146 2166 2739 2804 1870 Size: 258 18 20 573 65 4545 Fragments arranged by size: 4545 573 258 65 20 18 XmnI GAAnn'nnTTC Cuts at: 3608 3608 Size: 5479 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3287 276 Size: 53 83 186 936 1753 2468 Fragments arranged by size: 2468 1753 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FspAI NruI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SwaI XcmI