Restriction map for
pFN21AB7540
(Linear) (Six-base) MAPSORT of: KIBB7540.seq Check: 9092 from: 1 to: 774
KIBB7540.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 345 546 582 672 774
Size: 345 201 36 90 102
Fragments arranged by size:
345 201 102 90 36
ApaI G_GGCC'C
Cuts at: 0 273 575 734 774
Size: 273 302 159 40
Fragments arranged by size:
302 273 159 40
AvaI C'yCGr_G
Cuts at: 0 123 141 161 477 734 774
Size: 123 18 20 316 257 40
Fragments arranged by size:
316 257 123 40 20 18
BanI G'GyrC_C
Cuts at: 0 157 558 774
Size: 157 401 216
Fragments arranged by size:
401 216 157
BanII G_rGCy'C
Cuts at: 0 170 273 352 575 734 774
Size: 170 103 79 223 159 40
Fragments arranged by size:
223 170 159 103 79 40
BbeI G_GCGC'C
Cuts at: 0 161 774
Size: 161 613
BbsI GAAGACnn'nnnn_
Cuts at: 0 318 509 774
Size: 318 191 265
Fragments arranged by size:
318 265 191
BfrBI ATG'CAT
Cuts at: 0 769 774
Size: 769 5
BglI GCCn_nnn'nGGC
Cuts at: 0 137 774
Size: 137 637
Bme1580I G_kGCm'C
Cuts at: 0 273 553 561 575 734 774
Size: 273 280 8 14 159 40
Fragments arranged by size:
280 273 159 40 14 8
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 376 774
Size: 27 349 398
Fragments arranged by size:
398 349 27
Bpu10I CC'TnA_GC
Cuts at: 0 84 346 774
Size: 84 262 428
Fragments arranged by size:
428 262 84
BsaI GGTCTCn'nnnn_
Cuts at: 0 488 616 774
Size: 488 128 158
Fragments arranged by size:
488 158 128
BsaHI Gr'CG_yC
Cuts at: 0 158 323 676 774
Size: 158 165 353 98
Fragments arranged by size:
353 165 158 98
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 52 82 774
Size: 52 30 692
Fragments arranged by size:
692 52 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3 774
Size: 3 771
BseYI C'CCAG_C
Cuts at: 0 458 498 708 774
Size: 458 40 210 66
Fragments arranged by size:
458 210 66 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 774
Size: 448 326
Bsp1286I G_dGCh'C
Cuts at: 0 170 273 352 553 561 575 734 774
Size: 170 103 79 201 8 14 159 40
Fragments arranged by size:
201 170 159 103 79 40 14 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 294 774
Size: 294 480
BsrBI CCG'CTC
Cuts at: 0 360 774
Size: 360 414
BsrFI r'CCGG_y
Cuts at: 0 698 774
Size: 698 76
BssSI C'ACGA_G
Cuts at: 0 23 774
Size: 23 751
Bsu36I CC'TnA_GG
Cuts at: 0 173 774
Size: 173 601
BtgI C'CryG_G
Cuts at: 0 56 233 296 725 774
Size: 56 177 63 429 49
Fragments arranged by size:
429 177 63 56 49
DraIII CAC_nnn'GTG
Cuts at: 0 25 774
Size: 25 749
EaeI y'GGCC_r
Cuts at: 0 236 371 774
Size: 236 135 403
Fragments arranged by size:
403 236 135
EarI CTCTTCn'nnn_
Cuts at: 0 358 774
Size: 358 416
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 376 774
Size: 27 349 398
Fragments arranged by size:
398 349 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 692 774
Size: 692 82
EcoO109I rG'GnC_Cy
Cuts at: 0 203 270 453 572 633 730 731 738
Size: 203 67 183 119 61 97 1 7
Cuts at: 738 774
Size: 36
Fragments arranged by size:
203 183 119 97 67 61 36 7
1
FseI GG_CCGG'CC
Cuts at: 0 702 774
Size: 702 72
HaeII r_GCGC'y
Cuts at: 0 161 774
Size: 161 613
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 52 84 774
Size: 52 32 690
Fragments arranged by size:
690 52 32
KasI G'GCGC_C
Cuts at: 0 157 774
Size: 157 617
MscI TGG'CCA
Cuts at: 0 373 774
Size: 373 401
MspA1I CmG'CkG
Cuts at: 0 80 462 543 774
Size: 80 382 81 231
Fragments arranged by size:
382 231 81 80
NaeI GCC'GGC
Cuts at: 0 700 774
Size: 700 74
NarI GG'CG_CC
Cuts at: 0 158 774
Size: 158 616
NcoI C'CATG_G
Cuts at: 0 233 296 774
Size: 233 63 478
Fragments arranged by size:
478 233 63
NgoMIV G'CCGG_C
Cuts at: 0 698 774
Size: 698 76
NsiI A_TGCA'T
Cuts at: 0 771 774
Size: 771 3
PflMI CCAn_nnn'nTGG
Cuts at: 0 25 774
Size: 25 749
PpuMI rG'GwC_Cy
Cuts at: 0 203 453 774
Size: 203 250 321
Fragments arranged by size:
321 250 203
PspOMI G'GGCC_C
Cuts at: 0 269 571 730 774
Size: 269 302 159 44
Fragments arranged by size:
302 269 159 44
PstI C_TGCA'G
Cuts at: 0 542 774
Size: 542 232
PvuII CAG'CTG
Cuts at: 0 80 462 543 774
Size: 80 382 81 231
Fragments arranged by size:
382 231 81 80
SexAI A'CCwGG_T
Cuts at: 0 409 774
Size: 409 365
SfcI C'TryA_G
Cuts at: 0 538 774
Size: 538 236
SfoI GGC'GCC
Cuts at: 0 159 774
Size: 159 615
SmaI CCC'GGG
Cuts at: 0 125 143 163 736 774
Size: 125 18 20 573 38
Fragments arranged by size:
573 125 38 20 18
SrfI GCCC'GGGC
Cuts at: 0 143 774
Size: 143 631
StuI AGG'CCT
Cuts at: 0 108 774
Size: 108 666
StyI C'CwwG_G
Cuts at: 0 233 296 314 553 774
Size: 233 63 18 239 221
Fragments arranged by size:
239 233 221 63 18
XmaI C'CCGG_G
Cuts at: 0 123 141 161 734 774
Size: 123 18 20 573 40
Fragments arranged by size:
573 123 40 20 18
Enzymes that do cut:
AlwNI ApaI AvaI BanI BanII BbeI BbsI BfrBI
BglI Bme1580I BpmI Bpu10I BsaI BsaHI BsaXI BseRI
BseYI BsgI Bsp1286I BspMI BsrBI BsrFI BssSI Bsu36I
BtgI DraIII EaeI EarI Eco57MI EcoNI EcoO109I FseI
HaeII Hin4I KasI MscI MspA1I NaeI NarI NcoI
NgoMIV NsiI PflMI PpuMI PspOMI PstI PvuII SexAI
SfcI SfoI SmaI SrfI StuI StyI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BciVI BclI BglII BlpI BmgBI BmrI BmtI BplI
BpuEI BsaAI BsaBI BsaWI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BssHII BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgZI BtsI ClaI CspCI
DraI DrdI EagI EciI EcoICRI EcoRI EcoRV FalI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI MslI NdeI NheI NotI NruI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SgfI SgrAI SmlI SnaBI
SpeI SphI SspI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmnI ZraI