Restriction map for
pFN21AB7535
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I Cac8I CelII Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII HgaI Hin1I HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MspA1I MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI TspRI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 44 CGCG CG^CG
AfaI 213 GTAC GT^AC
AluI 86 AGCT AG^CT
ApaI 304 GGGCCC GGGCC^C
Asp700I 84 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 22 GAGCAC G[AT]GC[AT]^C
BanII 304 GGGCCC G[GA]GC[CT]^C
BbvI 36 GCTGC GCAGC(8/12)
BfaI 19 CTAG C^TAG
BsiHKAI 22 GAGCAC G[AT]GC[AT]^C
BspMI 109 ACCTGC ACCTGC(4/8)
BsrI 205 CCAGT ACTGG(1/-1)
BssKI 181 CCAGG ^CC[ACGT]GG
BstNI 181 CCAGG CC^[AT]GG
BstUI 44 CGCG CG^CG
BstYI 425 AGATCC [GA]^GATC[CT]
CfoI 45 GCGC GCG^C
DdeI 269 CTCAG C^T[ACGT]AG
DraI 238 TTTAAA TTT^AAA
EaeI 232 CGGCCA [CT]^GGCC[GA]
EcoRII 181 CCAGG ^CC[AT]GG
FauI 42 CCCGC CCCGC(4/6)
Fnu4HI 36 GCTGC GC^[ACGT]GC
HapII 231 CCGG C^CGG
HhaI 45 GCGC GCG^C
HinP1I 45 GCGC G^CGC
HincII 274 GTCAAC GT[CT]^[GA]AC
HindII 274 GTCAAC GT[CT]^[GA]AC
HpaII 231 CCGG C^CGG
ItaI 36 GCTGC GC^[ACGT]GC
MaeI 19 CTAG C^TAG
MflI 425 AGATCC [GA]^GATC[CT]
MslI 93 CACCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 231 CCGG C^CGG
MvaI 181 CCAGG CC^[AT]GG
MvnI 44 CGCG CG^CG
PleI 287 GACTC GAGTC(4/5)
RsaI 213 GTAC GT^AC
SapI 87 GCTCTTC GCTCTTC(1/4)
ScrFI 181 CCAGG CC^[ACGT]GG
SfaNI 256 GATGC GCATC(5/9)
SspI 157 AATATT AAT^ATT
TaqI 327 TCGA T^CGA
TfiI 408 GATTC G^A[AT]TC
TseI 36 GCTGC G^C[AT]GC
TthHB8I 327 TCGA T^CGA
XhoII 425 AGATCC [GA]^GATC[CT]
XmnI 84 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 43 CCGC CCGC(-3/-1)
429 CCGC
AvaII 107 GGACC G^G[AT]CC
446 GGACC
BmyI 22 GAGCAC G[AGT]GC[ACT]^C
304 GGGCCC
BsaJI 95 CCGAGG C^C[ACGT][ACGT]GG
186 CCGAGG
BsgI 37 CTGCAC GTGCAG(16/14)
111 CTGCAC
BsmFI 106 GGGAC GGGAC(10/14)
445 GGGAC
Bsp1286I 22 GAGCAC G[AGT]GC[ACT]^C
304 GGGCCC
DraII 106 GGGACCT [GA]G^G[ACGT]CC[CT]
445 GGGACCC
EarI 88 CTCTTC CTCTTC(1/4)
289 CTCTTC
EcoO109I 106 GGGACCT [GA]G^G[ACGT]CC[CT]
445 GGGACCC
HinfI 287 GACTC G^A[ACGT]TC
408 GATTC
Ksp632I 88 CTCTTC CTCTTC(1/4)
289 CTCTTC
MboII 89 TCTTC GAAGA(8/7)
290 TCTTC
MwoI 36 GCTGCACCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
47 GCTACTTCGGC
NlaIII 309 CATG CATG^
377 CATG
PpuMI 106 GGGACCT [GA]G^G[AT]CC[CT]
445 GGGACCC
Tsp45I 142 GTCAC ^GT[GC]AC
265 GTCAC
Tsp509I 135 AATT ^AATT
300 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AlwI BsiYI BslI BstF5I Cfr13I
DpnII FokI HaeIII HphI MaeIII
MboI MnlI MseI NdeII NlaIV
Sau3AI Sau96I Tru9I