(Circular) (Six-base) MAPSORT of: pFN21AB7535.seq Check: 4767 from: 1 to: 5221 pFN21AB7535 5221 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3031 278 Size: 53 83 186 936 1495 2468 Fragments arranged by size: 2468 1495 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2564 1937 Size: 627 4594 Acc65I G'GTAC_C Cuts at: 2542 2542 Size: 5221 AclI AA'CG_TT Cuts at: 1563 3348 3721 1563 Size: 1785 373 3063 Fragments arranged by size: 3063 1785 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2331 3284 4668 4936 1632 Size: 699 953 1384 268 1917 Fragments arranged by size: 1917 1384 953 699 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5024 AflIII A'CryG_T Cuts at: 1203 4120 5097 1203 Size: 2917 977 1327 Fragments arranged by size: 2917 1327 977 AgeI A'CCGG_T Cuts at: 4052 4052 Size: 5221 AhdI GACnn_n'nnGTC Cuts at: 3950 3950 Size: 5221 AleI CACnn'nnGTG Cuts at: 1166 2102 1166 Size: 936 4285 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5221 AlwNI CAG_nnn'CTG Cuts at: 2964 4536 2964 Size: 1572 3649 ApaI G_GGCC'C Cuts at: 2313 2313 Size: 5221 ApaLI G'TGCA_C Cuts at: 3278 4434 3278 Size: 1156 4065 ApoI r'AATT_y Cuts at: 1493 2530 2756 2908 5017 1493 Size: 1037 226 152 2109 1697 Fragments arranged by size: 2109 1697 1037 226 152 AseI AT'TA_AT Cuts at: 160 1032 3775 160 Size: 872 2743 1606 Fragments arranged by size: 2743 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5221 AvaI C'yCGr_G Cuts at: 1870 1943 2546 1870 Size: 73 603 4545 Fragments arranged by size: 4545 603 73 BamHI G'GATC_C Cuts at: 2551 2918 2551 Size: 367 4854 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2542 3997 618 Size: 325 217 627 755 1455 1842 Fragments arranged by size: 1842 1455 755 627 325 217 BanII G_rGCy'C Cuts at: 1391 2313 2540 1391 Size: 922 227 4072 Fragments arranged by size: 4072 922 227 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4250 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3412 3446 1903 Size: 34 1475 34 3678 Fragments arranged by size: 3678 1475 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3113 4323 3113 Size: 1210 4011 BclI T'GATC_A Cuts at: 1548 1548 Size: 5221 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3832 136 Size: 107 122 71 631 605 2160 1525 Fragments arranged by size: 2160 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5216 5216 Size: 5221 BlpI GC'TnA_GC Cuts at: 2638 2638 Size: 5221 Bme1580I G_kGCm'C Cuts at: 1790 2313 3282 4438 1790 Size: 523 969 1156 2573 Fragments arranged by size: 2573 1156 969 523 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5221 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2952 3910 442 Size: 416 53 83 399 24 1535 958 1753 Fragments arranged by size: 1753 1535 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5221 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3881 1239 Size: 2642 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3346 4211 4509 3346 Size: 865 298 4058 Fragments arranged by size: 4058 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3884 882 Size: 617 2385 2219 Fragments arranged by size: 2385 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3012 493 Size: 711 1808 2702 Fragments arranged by size: 2702 1808 711 BsaBI GATnn'nnATC Cuts at: 5215 5215 Size: 5221 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3028 3410 4045 275 Size: 53 83 186 936 1495 382 635 1451 Fragments arranged by size: 1495 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3654 4052 4326 4473 4884 3654 Size: 398 274 147 411 3991 Fragments arranged by size: 3991 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2003 2033 1178 Size: 30 165 30 600 30 4366 Fragments arranged by size: 4366 600 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5221 BseYI C'CCAG_C Cuts at: 4424 4920 4424 Size: 496 4725 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2027 2101 1163 Size: 573 291 74 4283 Fragments arranged by size: 4283 573 291 74 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2671 3432 4460 1540 Size: 275 186 670 761 1028 2301 Fragments arranged by size: 2301 1028 761 670 275 186 BsiHKAI G_wGCw'C Cuts at: 2031 3282 3367 4438 2031 Size: 1251 85 1071 2814 Fragments arranged by size: 2814 1251 1071 85 BsmI GAATG_Cn' Cuts at: 2732 2825 2732 Size: 93 5128 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2313 2540 3282 3367 4438 1391 Size: 399 241 282 227 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 282 241 227 85 BspEI T'CCGG_A Cuts at: 4884 4884 Size: 5221 BspHI T'CATG_A Cuts at: 3110 4840 3110 Size: 1730 3491 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2123 2576 844 Size: 1279 453 3489 Fragments arranged by size: 3489 1279 453 BsrBI CCG'CTC Cuts at: 1394 1823 3108 1394 Size: 429 1285 3507 Fragments arranged by size: 3507 1285 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3716 3890 65 Size: 1183 2468 174 1396 Fragments arranged by size: 2468 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3865 4052 1700 Size: 110 2055 187 2869 Fragments arranged by size: 2869 2055 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5221 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5221 BssSI C'ACGA_G Cuts at: 3281 4293 3281 Size: 1012 4209 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5221 BstBI TT'CG_AA Cuts at: 4025 4025 Size: 5221 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5221 BstYI r'GATC_y Cuts at: 1972 2430 2551 2918 3303 3320 4772 4858 4870 Size: 458 121 367 385 17 1452 86 12 Cuts at: 4870 5216 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 458 385 367 346 121 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4669 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4482 Fragments arranged by size: 4482 683 56 BtsI GCAGTG_nn' Cuts at: 2737 3531 3551 2737 Size: 794 20 4407 Fragments arranged by size: 4407 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5221 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5186 DraI TTT'AAA Cuts at: 2245 2525 2245 Size: 280 4941 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4228 809 Size: 507 2912 1802 Fragments arranged by size: 2912 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2237 2668 3557 Size: 54 1425 50 275 18 407 431 889 Cuts at: 3557 5186 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 431 407 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2668 1537 Size: 275 856 4090 Fragments arranged by size: 4090 856 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2099 2300 3151 1353 Size: 746 201 851 3423 Fragments arranged by size: 3423 851 746 201 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3808 4182 4328 387 Size: 1064 138 2219 374 146 1280 Fragments arranged by size: 2219 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2331 3284 3881 4668 4936 1239 Size: 393 699 953 597 787 268 1524 Fragments arranged by size: 1524 953 787 699 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2112 2451 2112 Size: 339 4882 EcoRI G'AATT_C Cuts at: 2530 2908 2530 Size: 378 4843 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2149 2149 Size: 5221 FspI TGC'GCA Cuts at: 3727 3727 Size: 5221 HaeII r_GCGC'y Cuts at: 1678 4368 1678 Size: 2690 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2184 2216 2325 2354 2357 Size: 32 135 32 612 32 109 29 3 Cuts at: 2357 2386 3862 3894 3936 3968 1373 Size: 29 1476 32 42 32 2626 Fragments arranged by size: 2626 1476 612 135 109 42 32 32 32 32 32 29 29 3 HincII GTy'rAC Cuts at: 669 1657 1938 2281 2565 2811 669 Size: 988 281 343 284 246 3079 Fragments arranged by size: 3079 988 343 284 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5221 HpaI GTT'AAC Cuts at: 2811 2811 Size: 5221 KpnI G_GTAC'C Cuts at: 2546 2546 Size: 5221 MfeI C'AATT_G Cuts at: 2820 2820 Size: 5221 MluI A'CGCG_T Cuts at: 5097 5097 Size: 5221 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2269 2397 4335 4519 1305 Size: 964 128 1938 184 2007 Fragments arranged by size: 2007 1938 964 184 128 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3399 Fragments arranged by size: 3399 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2102 3179 3538 3697 518 Size: 648 936 1077 359 159 2042 Fragments arranged by size: 2042 1077 936 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2637 3314 4462 4707 1606 Size: 1031 677 1148 245 2120 Fragments arranged by size: 2120 1148 1031 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4669 NdeI CA'TA_TG Cuts at: 387 387 Size: 5221 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5221 NotI GC'GGCC_GC Cuts at: 2668 2668 Size: 5221 NspI r_CATG'y Cuts at: 1132 1229 2579 4124 1132 Size: 97 1350 1545 2229 Fragments arranged by size: 2229 1545 1350 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5221 PciI A'CATG_T Cuts at: 4120 4120 Size: 5221 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5221 PmeI GTTT'AAAC Cuts at: 2525 2525 Size: 5221 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3290 3322 4803 4835 3290 Size: 32 1481 32 3676 Fragments arranged by size: 3676 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2112 2451 2112 Size: 339 4882 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5221 PsiI TTA'TAA Cuts at: 2791 2791 Size: 5221 PspOMI G'GGCC_C Cuts at: 2309 2309 Size: 5221 PstI C_TGCA'G Cuts at: 830 2573 830 Size: 1743 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5221 SalI G'TCGA_C Cuts at: 1936 2563 1936 Size: 627 4594 SanDI GG'GwC_CC Cuts at: 2451 2451 Size: 5221 SapI GCTCTTCn'nnn_ Cuts at: 2099 2099 Size: 5221 SbfI CC_TGCA'GG Cuts at: 2573 2573 Size: 5221 ScaI AGT'ACT Cuts at: 3469 5181 3469 Size: 1712 3509 SfcI C'TryA_G Cuts at: 826 1045 2569 3704 4385 4576 826 Size: 219 1524 1135 681 191 1471 Fragments arranged by size: 1524 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5221 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5221 SmaI CCC'GGG Cuts at: 1872 2548 1872 Size: 676 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3325 4226 4488 820 Size: 197 926 1382 901 262 1553 Fragments arranged by size: 1553 1382 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3012 493 Size: 2519 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5221 SphI G_CATG'C Cuts at: 1132 2579 1132 Size: 1447 3774 SspI AAT'ATT Cuts at: 5 52 2164 3145 5 Size: 47 2112 981 2081 Fragments arranged by size: 2112 2081 981 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5221 StyI C'CwwG_G Cuts at: 513 1065 2660 513 Size: 552 1595 3074 Fragments arranged by size: 3074 1595 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3248 3265 3418 3603 4048 325 Size: 1255 1668 17 153 185 445 1498 Fragments arranged by size: 1668 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2217 3467 5179 Size: 274 80 33 51 1443 240 1250 1712 Cuts at: 5179 96 Size: 138 Fragments arranged by size: 1712 1443 1250 274 240 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5181 5213 5181 Size: 32 5189 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5221 XbaI T'CTAG_A Cuts at: 2557 2557 Size: 5221 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5221 XmaI C'CCGG_G Cuts at: 1870 2546 1870 Size: 676 4545 XmnI GAAnn'nnTTC Cuts at: 2093 3350 2093 Size: 1257 3964 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3029 276 Size: 53 83 186 936 1495 2468 Fragments arranged by size: 2468 1495 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI