Restriction map for
pFN21AB7532
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsiEI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspHI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstF5I
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DpnII
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FokI FseI FspI HaeII HindIII
HinfI HpaI KasI KpnI KspI
MaeI MaeII MamI MboI MfeI
MflI MluI MluNI MscI MunI
MvnI NarI NdeI NdeII NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI Sau3AI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tsp509I Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccIII 24 TCCGGA T^CCGGA
AfaI 576 GTAC GT^AC
AflIII 362 ACATGT A^C[GA][CT]GT
AspHI 505 GAGCAC G[AT]GC[AT]^C
AvaII 190 GGTCC G^G[AT]CC
BanI 513 GGCACC G^G[CT][GA]CC
BbsI 661 GAAGAC GAAGAC(2/6)
BcgI 726 CGACGCCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 493 CTGGAG CTGGAG(16/14)
BpuAI 661 GAAGAC GAAGAC(2/6)
BsaWI 24 TCCGGA [AT]^CCGG[AT]
BseAI 24 TCCGGA T^CCGGA
BsiHKAI 505 GAGCAC G[AT]GC[AT]^C
BspEI 24 TCCGGA T^CCGGA
BspLU11I 362 ACATGT A^CATGT
BspMI 132 ACCTGC ACCTGC(4/8)
BsrFI 253 GCCGGC [GA]^CCGG[CT]
BstAPI 127 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 736 GCGC GCG^C
Cfr10I 253 GCCGGC [GA]^CCGG[CT]
EcoT14I 301 CCATGG C^C[AT][AT]GG
HhaI 736 GCGC GCG^C
HinP1I 736 GCGC G^CGC
MroI 24 TCCGGA T^CCGGA
MseI 740 TTAA T^TAA
NaeI 253 GCCGGC GCC^GGC
NcoI 301 CCATGG C^CATGG
NgoMIV 253 GCCGGC G^CCGGC
NspI 362 ACATGT [GA]CATG^[CT]
PpuMI 189 GGGTCCT [GA]G^G[AT]CC[CT]
RsaI 576 GTAC GT^AC
SapI 671 GAAGAGC GCTCTTC(1/4)
StyI 301 CCATGG C^C[AT][AT]GG
TaqI 695 TCGA T^CGA
Tru9I 740 TTAA T^TAA
TthHB8I 695 TCGA T^CGA
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# TWO CUT ENZYMES ( 17 enzymes) #
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AcyI 61 GGCGTC G[GA]^CG[CT]C
727 GACGCC
BbvCI 538 CCTCAGC CCTCAGC(-5/-2)
93 GCTGAGG
BsaHI 61 GGCGTC G[GA]^CG[CT]C
727 GACGCC
BsgI 134 CTGCAC GTGCAG(16/14)
370 GTGCAG
DsaI 301 CCATGG C^C[GA][CT]GG
532 CCGTGG
EaeI 49 CGGCCA [CT]^GGCC[GA]
278 CGGCCA
EarI 671 GAAGAG CTCTTC(1/4)
715 GAAGAG
FauI 475 CCCGC CCCGC(4/6)
489 CCCGC
HgaI 727 GACGC GACGC(5/10)
62 GCGTC
Hin1I 61 GGCGTC G[GA]^CG[CT]C
727 GACGCC
HincII 417 GTTGAC GT[CT]^[GA]AC
687 GTTGAC
HindII 417 GTTGAC GT[CT]^[GA]AC
687 GTTGAC
HphI 443 TCACC GGTGA(8/7)
521 TCACC
Ksp632I 671 GAAGAG CTCTTC(1/4)
715 GAAGAG
MaeIII 229 GTGAC ^GT[ACGT]AC
451 GTCAC
PvuII 342 CAGCTG CAG^CTG
356 CAGCTG
Tsp45I 229 GTGAC ^GT[GC]AC
451 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI AlwNI ApaI BanII
BbvI BcnI BmyI BsaJI BseRI
BsiYI BslI Bsp1286I BsrI BssKI
BstNI Cac8I Cfr13I DdeI DraII
EcoO109I EcoRII Fnu4HI HaeIII HapII
HpaII ItaI MboII MnlI MslI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV PstI Sau96I ScrFI
SfcI TseI TspRI XcmI