(Circular) (Six-base) MAPSORT of: pFN21AB7532.seq Check: 9357 from: 1 to: 5473 pFN21AB7532 5473 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2146 2146 Size: 5473 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3283 278 Size: 53 83 186 936 1747 2468 Fragments arranged by size: 2468 1747 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2816 1937 Size: 879 4594 Acc65I G'GTAC_C Cuts at: 2794 2794 Size: 5473 AclI AA'CG_TT Cuts at: 1563 3600 3973 1563 Size: 2037 373 3063 Fragments arranged by size: 3063 2037 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2639 3536 4920 5188 1632 Size: 1007 897 1384 268 1917 Fragments arranged by size: 1917 1384 1007 897 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5276 AflIII A'CryG_T Cuts at: 1203 2367 4372 5349 1203 Size: 1164 2005 977 1327 Fragments arranged by size: 2005 1327 1164 977 AgeI A'CCGG_T Cuts at: 4304 4304 Size: 5473 AhdI GACnn_n'nnGTC Cuts at: 4202 4202 Size: 5473 AleI CACnn'nnGTG Cuts at: 1166 2189 2402 1166 Size: 1023 213 4237 Fragments arranged by size: 4237 1023 213 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5473 AlwNI CAG_nnn'CTG Cuts at: 2138 2173 2299 3216 4788 2138 Size: 35 126 917 1572 2823 Fragments arranged by size: 2823 1572 917 126 35 ApaI G_GGCC'C Cuts at: 2412 2493 2507 2613 2412 Size: 81 14 106 5272 Fragments arranged by size: 5272 106 81 14 ApaLI G'TGCA_C Cuts at: 3530 4686 3530 Size: 1156 4317 ApoI r'AATT_y Cuts at: 1493 2782 3008 3160 5269 1493 Size: 1289 226 152 2109 1697 Fragments arranged by size: 2109 1697 1289 226 152 AseI AT'TA_AT Cuts at: 160 1032 4027 160 Size: 872 2995 1606 Fragments arranged by size: 2995 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5473 AvaI C'yCGr_G Cuts at: 1870 1943 2798 1870 Size: 73 855 4545 Fragments arranged by size: 4545 855 73 BamHI G'GATC_C Cuts at: 2803 3170 2803 Size: 367 5106 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2518 2794 4249 618 Size: 325 217 627 731 276 1455 1842 Fragments arranged by size: 1842 1455 731 627 325 276 217 BanII G_rGCy'C Cuts at: 1391 2412 2493 2507 2613 2792 1391 Size: 1021 81 14 106 179 4072 Fragments arranged by size: 4072 1021 179 106 81 14 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2673 928 Size: 971 774 3728 Fragments arranged by size: 3728 971 774 BbvCI CC'TCA_GC Cuts at: 2099 2544 2099 Size: 445 5028 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2720 2754 3664 3698 1903 Size: 34 50 34 699 34 910 34 3678 Fragments arranged by size: 3678 910 699 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3365 4575 3365 Size: 1210 4263 BclI T'GATC_A Cuts at: 1548 1548 Size: 5473 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4084 136 Size: 107 122 71 631 605 2412 1525 Fragments arranged by size: 2412 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5468 5468 Size: 5473 BlpI GC'TnA_GC Cuts at: 2890 2890 Size: 5473 Bme1580I G_kGCm'C Cuts at: 1790 2334 2390 2412 2493 2507 2613 3534 4690 Size: 544 56 22 81 14 106 921 1156 Cuts at: 4690 1790 Size: 2573 Fragments arranged by size: 2573 1156 921 544 106 81 56 22 14 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5473 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2608 3204 4162 Size: 416 53 83 399 24 1191 596 958 Cuts at: 4162 442 Size: 1753 Fragments arranged by size: 1753 1191 958 596 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5473 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2519 4133 1239 Size: 1280 1614 2579 Fragments arranged by size: 2579 1614 1280 Bpu10I CC'TnA_GC Cuts at: 2099 2544 2099 Size: 445 5028 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3598 4463 4761 3598 Size: 865 298 4310 Fragments arranged by size: 4310 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4136 882 Size: 617 2637 2219 Fragments arranged by size: 2637 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3264 493 Size: 711 2060 2702 Fragments arranged by size: 2702 2060 711 BsaBI GATnn'nnATC Cuts at: 5467 5467 Size: 5473 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2067 2733 3280 3662 Size: 53 83 186 936 534 666 547 382 Cuts at: 3662 4297 275 Size: 635 1451 Fragments arranged by size: 1451 936 666 635 547 534 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2029 3906 4304 4578 4725 5136 2029 Size: 1877 398 274 147 411 2366 Fragments arranged by size: 2366 1877 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5248 Fragments arranged by size: 5248 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2116 2255 2404 1186 Size: 930 139 149 4255 Fragments arranged by size: 4255 930 149 139 BseYI C'CCAG_C Cuts at: 2041 2271 2390 2596 4676 5172 2041 Size: 230 119 206 2080 496 2342 Fragments arranged by size: 2342 2080 496 230 206 119 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2124 2396 1163 Size: 573 388 272 4240 Fragments arranged by size: 4240 573 388 272 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2923 3684 4712 1540 Size: 275 186 922 761 1028 2301 Fragments arranged by size: 2301 1028 922 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2514 3534 3619 4690 2514 Size: 1020 85 1071 3297 Fragments arranged by size: 3297 1071 1020 85 BsmI GAATG_Cn' Cuts at: 2984 3077 2984 Size: 93 5380 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2334 2390 2412 2493 2507 2514 2613 Size: 399 544 56 22 81 14 7 99 Cuts at: 2613 2792 3534 3619 4690 1391 Size: 179 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 544 399 179 99 85 81 56 22 14 7 BspEI T'CCGG_A Cuts at: 2029 5136 2029 Size: 3107 2366 BspHI T'CATG_A Cuts at: 3362 5092 3362 Size: 1730 3743 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2146 2828 844 Size: 1302 682 3489 Fragments arranged by size: 3489 1302 682 BsrBI CCG'CTC Cuts at: 1394 1823 3360 1394 Size: 429 1537 3507 Fragments arranged by size: 3507 1537 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3968 4142 65 Size: 1183 2720 174 1396 Fragments arranged by size: 2720 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2258 4117 4304 1700 Size: 110 448 1859 187 2869 Fragments arranged by size: 2869 1859 448 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5473 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5473 BssSI C'ACGA_G Cuts at: 3533 4545 3533 Size: 1012 4461 BstAPI GCAn_nnn'nTGC Cuts at: 794 2138 794 Size: 1344 4129 BstBI TT'CG_AA Cuts at: 4277 4277 Size: 5473 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5473 BstYI r'GATC_y Cuts at: 1972 2803 3170 3555 3572 5024 5110 5122 5468 Size: 831 367 385 17 1452 86 12 346 Cuts at: 5468 1972 Size: 1977 Fragments arranged by size: 1977 1452 831 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2306 2537 513 Size: 552 1241 231 3449 Fragments arranged by size: 3449 1241 552 231 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4734 Fragments arranged by size: 4734 683 56 BtsI GCAGTG_nn' Cuts at: 2131 2238 2490 2989 3783 3803 2131 Size: 107 252 499 794 20 3801 Fragments arranged by size: 3801 794 499 252 107 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5473 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5438 DraI TTT'AAA Cuts at: 2777 2777 Size: 5473 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4480 809 Size: 507 3164 1802 Fragments arranged by size: 3164 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2054 2283 2920 Size: 54 1425 50 275 18 224 229 637 Cuts at: 2920 3809 5438 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 637 275 229 224 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2920 1537 Size: 275 1108 4090 Fragments arranged by size: 4090 1108 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2671 2715 3403 1353 Size: 1318 44 688 3423 Fragments arranged by size: 3423 1318 688 44 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2427 2461 4060 4434 4580 387 Size: 1064 138 838 34 1599 374 146 1280 Fragments arranged by size: 1599 1280 1064 838 374 146 138 34 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2519 2639 3536 4133 4920 5188 1239 Size: 393 887 120 897 597 787 268 1524 Fragments arranged by size: 1524 897 887 787 597 393 268 120 EcoO109I rG'GnC_Cy Cuts at: 2195 2408 2409 2489 2490 2503 2610 2195 Size: 213 1 80 1 13 107 5058 Fragments arranged by size: 5058 213 107 80 13 1 1 EcoRI G'AATT_C Cuts at: 2782 3160 2782 Size: 378 5095 FspI TGC'GCA Cuts at: 3979 3979 Size: 5473 HaeII r_GCGC'y Cuts at: 1678 4620 1678 Size: 2942 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4114 4146 4188 4220 1373 Size: 32 135 32 2542 32 42 32 2626 Fragments arranged by size: 2626 2542 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2424 2694 2817 3063 669 Size: 988 281 486 270 123 246 3079 Fragments arranged by size: 3079 988 486 281 270 246 123 HindIII A'AGCT_T Cuts at: 748 748 Size: 5473 HpaI GTT'AAC Cuts at: 3063 3063 Size: 5473 KpnI G_GTAC'C Cuts at: 2798 2798 Size: 5473 MfeI C'AATT_G Cuts at: 3072 3072 Size: 5473 MluI A'CGCG_T Cuts at: 5349 5349 Size: 5473 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4587 4771 1305 Size: 3282 184 2007 Fragments arranged by size: 3282 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3651 Fragments arranged by size: 3651 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2189 2372 2402 3431 3790 3949 518 Size: 648 1023 183 30 1029 359 159 2042 Fragments arranged by size: 2042 1029 1023 648 359 183 159 30 MspA1I CmG'CkG Cuts at: 1606 2349 2363 2497 2889 3566 4714 4959 1606 Size: 743 14 134 392 677 1148 245 2120 Fragments arranged by size: 2120 1148 743 677 392 245 134 14 NaeI GCC'GGC Cuts at: 2260 2260 Size: 5473 NcoI C'CATG_G Cuts at: 513 1065 2306 513 Size: 552 1241 3680 Fragments arranged by size: 3680 1241 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5473 NgoMIV G'CCGG_C Cuts at: 2258 2258 Size: 5473 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5473 NotI GC'GGCC_GC Cuts at: 2920 2920 Size: 5473 NspI r_CATG'y Cuts at: 1132 1229 2371 2831 4376 1132 Size: 97 1142 460 1545 2229 Fragments arranged by size: 2229 1545 1142 460 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5473 PciI A'CATG_T Cuts at: 2367 4372 2367 Size: 2005 3468 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 4571 PmeI GTTT'AAAC Cuts at: 2777 2777 Size: 5473 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3542 3574 5055 5087 3542 Size: 32 1481 32 3928 Fragments arranged by size: 3928 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2195 2195 Size: 5473 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5473 PsiI TTA'TAA Cuts at: 3043 3043 Size: 5473 PspOMI G'GGCC_C Cuts at: 2408 2489 2503 2609 2408 Size: 81 14 106 5272 Fragments arranged by size: 5272 106 81 14 PstI C_TGCA'G Cuts at: 830 2233 2295 2580 2825 830 Size: 1403 62 285 245 3478 Fragments arranged by size: 3478 1403 285 245 62 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5473 PvuII CAG'CTG Cuts at: 2349 2363 2349 Size: 14 5459 SalI G'TCGA_C Cuts at: 1936 2815 1936 Size: 879 4594 SapI GCTCTTCn'nnn_ Cuts at: 2671 2671 Size: 5473 SbfI CC_TGCA'GG Cuts at: 2825 2825 Size: 5473 ScaI AGT'ACT Cuts at: 3721 5433 3721 Size: 1712 3761 SfcI C'TryA_G Cuts at: 826 1045 2229 2291 2576 2821 3956 4637 4828 Size: 219 1184 62 285 245 1135 681 191 Cuts at: 4828 826 Size: 1471 Fragments arranged by size: 1471 1184 1135 681 285 245 219 191 62 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5473 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5473 SmaI CCC'GGG Cuts at: 1872 2800 1872 Size: 928 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3577 4478 4740 820 Size: 197 926 1634 901 262 1553 Fragments arranged by size: 1634 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3264 493 Size: 2771 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5473 SphI G_CATG'C Cuts at: 1132 2831 1132 Size: 1699 3774 SspI AAT'ATT Cuts at: 5 52 3397 5 Size: 47 3345 2081 Fragments arranged by size: 3345 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5473 StyI C'CwwG_G Cuts at: 513 1065 2306 2912 513 Size: 552 1241 606 3074 Fragments arranged by size: 3074 1241 606 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3500 3517 3670 3855 4300 325 Size: 1255 1920 17 153 185 445 1498 Fragments arranged by size: 1920 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3719 5431 96 Size: 274 80 33 51 1443 1742 1712 138 Fragments arranged by size: 1742 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2179 2211 5433 5465 2179 Size: 32 3222 32 2187 Fragments arranged by size: 3222 2187 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5473 XbaI T'CTAG_A Cuts at: 2809 2809 Size: 5473 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2123 2398 2482 2123 Size: 275 84 5114 Fragments arranged by size: 5114 275 84 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5473 XmaI C'CCGG_G Cuts at: 1870 2798 1870 Size: 928 4545 XmnI GAAnn'nnTTC Cuts at: 3602 3602 Size: 5473 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3281 276 Size: 53 83 186 936 1747 2468 Fragments arranged by size: 2468 1747 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI