Restriction map for
pFN21AB7525
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII AccII AccIII AclI AcyI
AflII AgeI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MseI
MslI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StyI SwaI Tru9I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 202 GGTACC G^GTACC
AccI 148 GTAGAC GT^[AC][GT]AC
AciI 4 GCGG CCGC(-3/-1)
AcsI 137 AAATTT [GA]^AATT[CT]
AflIII 344 ACGTGT A^C[GA][CT]GT
AhdI 67 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 111 GGATC GGATC(4/5)
ApoI 137 AAATTT [GA]^AATT[CT]
Asp718I 202 GGTACC G^GTACC
AspEI 67 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 202 GGTACC G^G[CT][GA]CC
BcnI 48 CCCGG CC^[GC]GG
BpmI 414 CTCCAG CTGGAG(16/14)
BsaAI 343 TACGTG [CT]AC^GT[GA]
BsiWI 341 CGTACG C^GTACG
BsiYI 12 CCTGTTTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 12 CCTGTTTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 75 GTCTC GTCTC(1/5)
Bsp1407I 287 TGTACA T^GTACA
BspHI 365 TCATGA T^CATGA
BspMI 392 ACCTGC ACCTGC(4/8)
BsrGI 287 TGTACA T^GTACA
BsrI 258 CCAGT ACTGG(1/-1)
BstYI 111 GGATCT [GA]^GATC[CT]
Cfr13I 357 GGGCC G^G[ACGT]CC
DdeI 120 CTTAG C^T[ACGT]AG
DpnII 112 GATC ^GATC
DraII 356 GGGGCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 67 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 12 CCTGTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 356 GGGGCCT [GA]G^G[ACGT]CC[CT]
FauI 4 GCGGG CCCGC(4/6)
HapII 49 CCGG C^CGG
HpaII 49 CCGG C^CGG
KpnI 202 GGTACC GGTAC^C
MaeII 344 ACGT A^CGT
MaeIII 158 GTGAC ^GT[ACGT]AC
MboI 112 GATC ^GATC
MflI 111 GGATCT [GA]^GATC[CT]
MspI 49 CCGG C^CGG
MwoI 363 GCTCATGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 48 CCCGG CC^[GC]GG
NdeII 112 GATC ^GATC
NheI 441 GCTAGC G^CTAGC
PleI 412 GACTC GAGTC(4/5)
PstI 373 CTGCAG CTGCA^G
RcaI 365 TCATGA T^CATGA
Sau3AI 112 GATC ^GATC
Sau96I 357 GGGCC G^G[ACGT]CC
SspBI 287 TGTACA T^GTACA
StuI 241 AGGCCT AGG^CCT
Tsp45I 158 GTGAC ^GT[GC]AC
Tsp509I 138 AATT ^AATT
TspRI 231 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 111 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AluI 180 AGCT AG^CT
440 AGCT
BbvI 322 GCAGC GCAGC(8/12)
372 GCTGC
BfaI 182 CTAG C^TAG
442 CTAG
BsaJI 206 CCCTGG C^C[ACGT][ACGT]GG
248 CCAGGG
BstNI 207 CCTGG CC^[AT]GG
248 CCAGG
EcoRII 207 CCTGG ^CC[AT]GG
248 CCAGG
Fnu4HI 322 GCAGC GC^[ACGT]GC
372 GCTGC
HaeIII 242 GGCC GG^CC
358 GGCC
HphI 157 GGTGA GGTGA(8/7)
217 GGTGA
ItaI 322 GCAGC GC^[ACGT]GC
372 GCTGC
MaeI 182 CTAG C^TAG
442 CTAG
MboII 196 GAAGA GAAGA(8/7)
435 GAAGA
MvaI 207 CCTGG CC^[AT]GG
248 CCAGG
NlaIII 366 CATG CATG^
397 CATG
NlaIV 202 GGTACC GG[ACGT]^[ACGT]CC
356 GGGGCC
SfaNI 382 GATGC GCATC(5/9)
8 GCATC
SfcI 146 CTGTAG C^T[GA][CT]AG
373 CTGCAG
TfiI 97 GAATC G^A[AT]TC
401 GATTC
TseI 322 GCAGC G^C[AT]GC
372 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AfaI BssKI BstF5I Cac8I FokI
HinfI MnlI RsaI ScrFI TaqI
TthHB8I