Restriction map for pFN21AB7525
 (Circular) (Six-base) MAPSORT of: pFN21AB7525.seq  Check: 9979  from: 1  to: 5155



pFN21AB7525  5155 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:39  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2965     278 
   Size:         53      83     186     936    1429    2468

  Fragments arranged by size: 

                2468    1429     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2154    2498    1937 
   Size:        217     344    4594

  Fragments arranged by size: 

                4594     344     217
 
Acc65I G'GTAC_C

Cuts at:   2207    2476    2207 
   Size:        269    4886
 
AclI AA'CG_TT

Cuts at:   1563    3282    3655    1563 
   Size:       1719     373    3063

  Fragments arranged by size: 

                3063    1719     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2343    3218    4602    4870    1632 
   Size:        711     875    1384     268    1917

  Fragments arranged by size: 

                1917    1384     875     711     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4958
 
AflIII A'CryG_T

Cuts at:   1203    2349    4054    5031    1203 
   Size:       1146    1705     977    1327

  Fragments arranged by size: 

                1705    1327    1146     977
 
AgeI A'CCGG_T

Cuts at:   3986    3986 
   Size:       5155
 
AhdI GACnn_n'nnGTC

Cuts at:   2077    3884    2077 
   Size:       1807    3348
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5155
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2403    1184 
   Size:       1219    3936
 
AlwNI CAG_nnn'CTG

Cuts at:   2898    4470    2898 
   Size:       1572    3583
 
ApaLI G'TGCA_C

Cuts at:   3212    4368    3212 
   Size:       1156    3999
 
ApoI r'AATT_y

Cuts at:   1493    2142    2464    2690    2842    4951    1493 
   Size:        649     322     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     649     322     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3709     160 
   Size:        872    2677    1606

  Fragments arranged by size: 

                2677    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5155
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2480    1870 
   Size:         73     537    4545

  Fragments arranged by size: 

                4545     537      73
 
BamHI G'GATC_C

Cuts at:   2485    2852    2485 
   Size:        367    4788
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2207    2476    3931     618 
   Size:        325     217     627     420     269    1455    1842

  Fragments arranged by size: 

                1842    1455     627     420     325     269     217
 
BanII G_rGCy'C

Cuts at:   1391    2474    1391 
   Size:       1083    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4184
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3346    3380    1903 
   Size:         34    1409      34    3678

  Fragments arranged by size: 

                3678    1409      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3047    4257    3047 
   Size:       1210    3945
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5155
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3766     136 
   Size:        107     122      71     631     605    2094    1525

  Fragments arranged by size: 

                2094    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5150    5150 
   Size:       5155
 
BlpI GC'TnA_GC

Cuts at:   2572    2572 
   Size:       5155
 
Bme1580I G_kGCm'C

Cuts at:   1790    3216    4372    1790 
   Size:       1426    1156    2573

  Fragments arranged by size: 

                2573    1426    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5155
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2886    3844     442 
   Size:        416      53      83     399      24    1469     958    1753

  Fragments arranged by size: 

                1753    1469     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    2450    1056 
   Size:       1394    3761
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2434    2447    2434 
   Size:         13    5142
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2404    3815    1239 
   Size:       1165    1411    2579

  Fragments arranged by size: 

                2579    1411    1165
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3280    4145    4443    3280 
   Size:        865     298    3992

  Fragments arranged by size: 

                3992     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3818     882 
   Size:        617    2319    2219

  Fragments arranged by size: 

                2319    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2350    2946     493 
   Size:        711    1146     596    2702

  Fragments arranged by size: 

                2702    1146     711     596
 
BsaBI GATnn'nnATC

Cuts at:   5149    5149 
   Size:       5155
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2962    3344    3979     275 
   Size:         53      83     186     936    1429     382     635    1451

  Fragments arranged by size: 

                1451    1429     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3588    3986    4260    4407    4818    3588 
   Size:        398     274     147     411    3925

  Fragments arranged by size: 

                3925     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4930

  Fragments arranged by size: 

                4930     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5155
 
BseYI C'CCAG_C

Cuts at:   4358    4854    4358 
   Size:        496    4659
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4582
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2605    3366    4394    1540 
   Size:        275     186     604     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     604     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3216    3301    4372    3216 
   Size:         85    1071    3999

  Fragments arranged by size: 

                3999    1071      85
 
BsiWI C'GTAC_G

Cuts at:   2346    2346 
   Size:       5155
 
BsmI GAATG_Cn'

Cuts at:   2666    2759    2666 
   Size:         93    5062
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2474    3216    3301    4372    1391 
   Size:        399     684     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     684     399      85
 
BspEI T'CCGG_A

Cuts at:   4818    4818 
   Size:       5155
 
BspHI T'CATG_A

Cuts at:   2370    3044    4774    2370 
   Size:        674    1730    2751

  Fragments arranged by size: 

                2751    1730     674
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2406    2510     844 
   Size:       1562     104    3489

  Fragments arranged by size: 

                3489    1562     104
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3042    1394 
   Size:        429    1219    3507

  Fragments arranged by size: 

                3507    1219     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3650    3824      65 
   Size:       1183    2402     174    1396

  Fragments arranged by size: 

                2402    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3799    3986    1700 
   Size:        110    1989     187    2869

  Fragments arranged by size: 

                2869    1989     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2292      96 
   Size:       2196    2959
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5155
 
BssSI C'ACGA_G

Cuts at:   3215    4227    3215 
   Size:       1012    4143
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5155
 
BstBI TT'CG_AA

Cuts at:   3959    3959 
   Size:       5155
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5155
 
BstYI r'GATC_y

Cuts at:   1972    2116    2485    2852    3237    3254    4706    4792    4804 
   Size:        144     369     367     385      17    1452      86      12

Cuts at:   4804    5150    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     385     369     367     346     144      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3664

  Fragments arranged by size: 

                3664     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4416

  Fragments arranged by size: 

                4416     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2671    3465    3485    2671 
   Size:        794      20    4341

  Fragments arranged by size: 

                4341     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5155
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5120
 
DraI TTT'AAA

Cuts at:   2459    2459 
   Size:       5155
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4162     809 
   Size:        507    2846    1802

  Fragments arranged by size: 

                2846    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2602    3491    5120 
   Size:         54    1425      50     275      18     772     889    1629

Cuts at:   5120       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     772     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2602    1537 
   Size:        275     790    4090

  Fragments arranged by size: 

                4090     790     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3085    1353 
   Size:       1732    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3742    4116    4262     387 
   Size:       1064     138    2153     374     146    1280

  Fragments arranged by size: 

                2153    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2343    2404    3218    3815    4602    4870    1239 
   Size:        393     711      61     814     597     787     268    1524

  Fragments arranged by size: 

                1524     814     787     711     597     393     268      61
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2021    2021 
   Size:       5155
 
EcoO109I rG'GnC_Cy

Cuts at:   2362    2362 
   Size:       5155
 
EcoRI G'AATT_C

Cuts at:   2464    2842    2464 
   Size:        378    4777
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2467    2467 
   Size:       5155
 
FspI TGC'GCA

Cuts at:   3661    3661 
   Size:       5155
 
HaeII r_GCGC'y

Cuts at:   1678    4302    1678 
   Size:       2624    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2063    2087    2095    2119    3796 
   Size:         32     135      32     491      24       8      24    1677

Cuts at:   3796    3828    3870    3902    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1677     491     135      42      32      32      32
                  32      24      24       8
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2499    2745     669 
   Size:        988     281     561     246    3079

  Fragments arranged by size: 

                3079     988     561     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5155
 
HpaI GTT'AAC

Cuts at:   2745    2745 
   Size:       5155
 
KpnI G_GTAC'C

Cuts at:   2211    2480    2211 
   Size:        269    4886
 
MfeI C'AATT_G

Cuts at:   2754    2754 
   Size:       5155
 
MluI A'CGCG_T

Cuts at:   5031    5031 
   Size:       5155
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4269    4453    1305 
   Size:       2964     184    2007

  Fragments arranged by size: 

                2964    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3333

  Fragments arranged by size: 

                3333    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3113    3472    3631     518 
   Size:        648    1947     359     159    2042

  Fragments arranged by size: 

                2042    1947     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2571    3248    4396    4641    1606 
   Size:        965     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     965     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3664

  Fragments arranged by size: 

                3664     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5155
 
NheI G'CTAG_C

Cuts at:   1052    2446    1052 
   Size:       1394    3761
 
NotI GC'GGCC_GC

Cuts at:   2602    2602 
   Size:       5155
 
NspI r_CATG'y

Cuts at:   1132    1229    2513    4058    1132 
   Size:         97    1284    1545    2229

  Fragments arranged by size: 

                2229    1545    1284      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5155
 
PciI A'CATG_T

Cuts at:   4054    4054 
   Size:       5155
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5155
 
PmeI GTTT'AAAC

Cuts at:   2459    2459 
   Size:       5155
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3224    3256    4737    4769    3224 
   Size:         32    1481      32    3610

  Fragments arranged by size: 

                3610    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5155
 
PsiI TTA'TAA

Cuts at:   2725    2725 
   Size:       5155
 
PstI C_TGCA'G

Cuts at:    830    2382    2507     830 
   Size:       1552     125    3478

  Fragments arranged by size: 

                3478    1552     125
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5155
 
SalI G'TCGA_C

Cuts at:   1936    2497    1936 
   Size:        561    4594
 
SbfI CC_TGCA'GG

Cuts at:   2507    2507 
   Size:       5155
 
ScaI AGT'ACT

Cuts at:   3403    5115    3403 
   Size:       1712    3443
 
SfcI C'TryA_G

Cuts at:    826    1045    2151    2378    2503    3638    4319    4510     826 
   Size:        219    1106     227     125    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1106     681     227     219     191     125
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5155
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5155
 
SmaI CCC'GGG

Cuts at:   1872    2482    1872 
   Size:        610    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3259    4160    4422     820 
   Size:        197     926    1316     901     262    1553

  Fragments arranged by size: 

                1553    1316     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2946     493 
   Size:       2453    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5155
 
SphI G_CATG'C

Cuts at:   1132    2513    1132 
   Size:       1381    3774
 
SspI AAT'ATT

Cuts at:      5      52    3079       5 
   Size:         47    3027    2081

  Fragments arranged by size: 

                3027    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2248    1517 
   Size:        731    4424
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2594     513 
   Size:        552     939     590    3074

  Fragments arranged by size: 

                3074     939     590     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2088    2150    3182    3199    3352    3537    3982 
   Size:       1255     508      62    1032      17     153     185     445

Cuts at:   3982     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1255    1032     508     445     185     153      62
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2292    3401    5113 
   Size:        274      80      33      51    1443     315    1109    1712

Cuts at:   5113      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1109     315     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2397    2429    5115    5147    2397 
   Size:         32    2686      32    2405

  Fragments arranged by size: 

                2686    2405      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5155
 
XbaI T'CTAG_A

Cuts at:   2491    2491 
   Size:       5155
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5155
 
XmaI C'CCGG_G

Cuts at:   1870    2480    1870 
   Size:        610    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3284    3284 
   Size:       5155
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2963     276 
   Size:         53      83     186     936    1429    2468

  Fragments arranged by size: 

                2468    1429     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI
     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI   Bpu10I    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoRV     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PfoI     PmlI    PpuMI   PspOMI    PspXI
     PsrI    PvuII    RsrII     SacI    SacII    SanDI     SapI    SexAI
     SfiI     SfoI     SrfI     SwaI     XcmI
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