Restriction map for
pFN21AB7516
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# NO CUT ENZYMES ( 188 enzymes) #
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AatII Acc65I AccII AccIII AcsI
AcyI AfaI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr13I ClaI CpoI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI Fnu4HI FokI
FseI FspI HaeII HaeIII HgaI
Hin1I HindIII ItaI KasI KpnI
KspI MaeI MaeIII MamI MfeI
MluI MluNI MroI MscI MslI
MspA1I MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SapI
Sau96I SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI StyI SwaI TseI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 80 GTCGAC GT^[AC][GT]AC
AclI 487 AACGTT AA^CGTT
Alw44I 63 GTGCAC G^TGCAC
AlwI 684 GGATC GGATC(4/5)
ApaLI 63 GTGCAC G^TGCAC
AspHI 63 GTGCAC G[AT]GC[AT]^C
BbsI 546 GAAGAC GAAGAC(2/6)
BbvCI 727 GCTGAGG CCTCAGC(-5/-2)
BpmI 50 CTGGAG CTGGAG(16/14)
BpuAI 546 GAAGAC GAAGAC(2/6)
BseRI 730 GAGGAG GAGGAG(10/8)
BsiHKAI 63 GTGCAC G[AT]GC[AT]^C
BspMI 321 GCAGGT ACCTGC(4/8)
BsrFI 67 ACCGGC [GA]^CCGG[CT]
BsrI 563 ACTGG ACTGG(1/-1)
BssKI 39 CCTGG ^CC[ACGT]GG
BstNI 39 CCTGG CC^[AT]GG
BstYI 684 GGATCT [GA]^GATC[CT]
CfoI 87 GCGC GCG^C
Cfr10I 67 ACCGGC [GA]^CCGG[CT]
DpnII 685 GATC ^GATC
EarI 334 GAAGAG CTCTTC(1/4)
EcoRII 39 CCTGG ^CC[AT]GG
HapII 68 CCGG C^CGG
HhaI 87 GCGC GCG^C
HinP1I 87 GCGC G^CGC
HpaI 444 GTTAAC GTT^AAC
HpaII 68 CCGG C^CGG
Ksp632I 334 GAAGAG CTCTTC(1/4)
MboI 685 GATC ^GATC
MflI 684 GGATCT [GA]^GATC[CT]
MspI 68 CCGG C^CGG
MvaI 39 CCTGG CC^[AT]GG
NdeII 685 GATC ^GATC
PleI 580 GACTC GAGTC(4/5)
Psp1406I 487 AACGTT AA^CGTT
SalI 80 GTCGAC G^TCGAC
Sau3AI 685 GATC ^GATC
ScrFI 39 CCTGG CC^[ACGT]GG
SfcI 230 CTACAG C^T[GA][CT]AG
SphI 606 GCATGC GCATG^C
TspRI 449 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 684 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AciI 532 CCGC CCGC(-3/-1)
313 GCGG
BmyI 35 GTGCCC G[AGT]GC[ACT]^C
63 GTGCAC
BsiYI 38 CCCTGGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
254 CCCAGCGTTGG
BslI 38 CCCTGGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
254 CCCAGCGTTGG
Bsp1286I 35 GTGCCC G[AGT]GC[ACT]^C
63 GTGCAC
Cac8I 26 GCGTGC GC[ACGT]^[ACGT]GC
606 GCATGC
HincII 80 GTCGAC GT[CT]^[GA]AC
444 GTTAAC
HindII 80 GTCGAC GT[CT]^[GA]AC
444 GTTAAC
HphI 523 GGTGA GGTGA(8/7)
529 TCACC
MaeII 488 ACGT A^CGT
693 ACGT
NspI 17 GCATGT [GA]CATG^[CT]
606 GCATGC
SfaNI 220 GATGC GCATC(5/9)
301 GATGC
TaqI 81 TCGA T^CGA
174 TCGA
TthHB8I 81 TCGA T^CGA
174 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
AluI BsaJI DdeI DraI HinfI
MboII MnlI MseI MwoI NlaIII
TfiI Tru9I Tsp509I