Restriction map for pFN21AB7515
 (Circular) (Six-base) MAPSORT of: pFN21AB7515.seq  Check: 6552  from: 1  to: 5086



pFN21AB7515  5086 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:39  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2896     278 
   Size:         53      83     186     936    1360    2468

  Fragments arranged by size: 

                2468    1360     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2429    1937 
   Size:        492    4594
 
Acc65I G'GTAC_C

Cuts at:   2407    2407 
   Size:       5086
 
AclI AA'CG_TT

Cuts at:   1563    3213    3586    1563 
   Size:       1650     373    3063

  Fragments arranged by size: 

                3063    1650     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2288    2309    3149    4533    4801    1632 
   Size:        656      21     840    1384     268    1917

  Fragments arranged by size: 

                1917    1384     840     656     268      21
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4889
 
AflIII A'CryG_T

Cuts at:   1203    3985    4962    1203 
   Size:       2782     977    1327

  Fragments arranged by size: 

                2782    1327     977
 
AgeI A'CCGG_T

Cuts at:   3917    3917 
   Size:       5086
 
AhdI GACnn_n'nnGTC

Cuts at:   3815    3815 
   Size:       5086
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5086
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2054    1184 
   Size:        870    4216
 
AlwNI CAG_nnn'CTG

Cuts at:   2829    4401    2829 
   Size:       1572    3514
 
ApaLI G'TGCA_C

Cuts at:   3143    4299    3143 
   Size:       1156    3930
 
ApoI r'AATT_y

Cuts at:   1493    2395    2621    2773    4882    1493 
   Size:        902     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     902     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3640     160 
   Size:        872    2608    1606

  Fragments arranged by size: 

                2608    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5086
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2060    2382    2411    1870 
   Size:         73     117     322      29    4545

  Fragments arranged by size: 

                4545     322     117      73      29
 
BamHI G'GATC_C

Cuts at:   2416    2783    2416 
   Size:        367    4719
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2056    2407    3862     618 
   Size:        325     217     627     269     351    1455    1842

  Fragments arranged by size: 

                1842    1455     627     351     325     269     217
 
BanII G_rGCy'C

Cuts at:   1391    2019    2099    2405    1391 
   Size:        628      80     306    4072

  Fragments arranged by size: 

                4072     628     306      80
 
BbeI G_GCGC'C

Cuts at:   2060    2060 
   Size:       5086
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4115
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3277    3311    1903 
   Size:         34    1340      34    3678

  Fragments arranged by size: 

                3678    1340      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2978    4188    2978 
   Size:       1210    3876
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5086
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2353    3697     136 
   Size:        107     122      71     631     605     681    1344    1525

  Fragments arranged by size: 

                1525    1344     681     631     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5081    5081 
   Size:       5086
 
BlpI GC'TnA_GC

Cuts at:   2503    2503 
   Size:       5086
 
Bme1580I G_kGCm'C

Cuts at:   1790    2312    3147    4303    1790 
   Size:        522     835    1156    2573

  Fragments arranged by size: 

                2573    1156     835     522
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5086
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2817    3775     442 
   Size:        416      53      83     399      24    1400     958    1753

  Fragments arranged by size: 

                1753    1400     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5086
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2234    3746    1239 
   Size:        995    1512    2579

  Fragments arranged by size: 

                2579    1512     995
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3211    4076    4374    3211 
   Size:        865     298    3923

  Fragments arranged by size: 

                3923     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3749     882 
   Size:        617    2250    2219

  Fragments arranged by size: 

                2250    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2877     493 
   Size:        711    1673    2702

  Fragments arranged by size: 

                2702    1673     711
 
BsaBI GATnn'nnATC

Cuts at:   5080    5080 
   Size:       5086
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2057    2893    3275    3910 
   Size:         53      83     186     936     524     836     382     635

Cuts at:   3910     275 
   Size:       1451

  Fragments arranged by size: 

                1451     936     836     635     524     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   3519    3917    4191    4338    4749    3519 
   Size:        398     274     147     411    3856

  Fragments arranged by size: 

                3856     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4861

  Fragments arranged by size: 

                4861     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2059    2276    1186 
   Size:        873     217    3996

  Fragments arranged by size: 

                3996     873     217
 
BseYI C'CCAG_C

Cuts at:   4289    4785    4289 
   Size:        496    4590
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4513
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2025    2357    2536    3297    4325    1540 
   Size:        275     186      24     332     179     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     332     275     186     179      24
            
 
BsiHKAI G_wGCw'C

Cuts at:   2298    3147    3232    4303    2298 
   Size:        849      85    1071    3081

  Fragments arranged by size: 

                3081    1071     849      85
 
BsmI GAATG_Cn'

Cuts at:   2597    2690    2597 
   Size:         93    4993
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2019    2099    2298    2312    2405    3147    3232 
   Size:        399     229      80     199      14      93     742      85

Cuts at:   3232    4303    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     229     199      93      85
                  80      14
 
BspEI T'CCGG_A

Cuts at:   4749    4749 
   Size:       5086
 
BspHI T'CATG_A

Cuts at:   2975    4705    2975 
   Size:       1730    3356
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2441     844 
   Size:       1597    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2973    1394 
   Size:        429    1150    3507

  Fragments arranged by size: 

                3507    1150     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3581    3755      65 
   Size:       1183    2333     174    1396

  Fragments arranged by size: 

                2333    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2027    2369    3730    3917    1700 
   Size:        110     217     342    1361     187    2869

  Fragments arranged by size: 

                2869    1361     342     217     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5086
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5086
 
BssSI C'ACGA_G

Cuts at:   2297    3146    4158    2297 
   Size:        849    1012    3225

  Fragments arranged by size: 

                3225    1012     849
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5086
 
BstBI TT'CG_AA

Cuts at:   3890    3890 
   Size:       5086
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5086
 
BstYI r'GATC_y

Cuts at:   1972    2416    2783    3168    3185    4637    4723    4735    5081 
   Size:        444     367     385      17    1452      86      12     346

Cuts at:   5081    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     444     385     367     346      86      17
                  12
 
Bsu36I CC'TnA_GG

Cuts at:   2100    2100 
   Size:       5086
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    4534
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4347

  Fragments arranged by size: 

                4347     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2602    3396    3416    2602 
   Size:        794      20    4272

  Fragments arranged by size: 

                4272     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5086
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5051
 
DraI TTT'AAA

Cuts at:   2390    2390 
   Size:       5086
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2117    4093     809 
   Size:        507     801    1976    1802

  Fragments arranged by size: 

                1976    1802     801     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2022    2354    2367 
   Size:         54    1425      50     275      18     192     332      13

Cuts at:   2367    2533    3422    5051       8 
   Size:        166     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     332     275     192     166      54
                  50      43      18      13
 
EagI C'GGCC_G

Cuts at:   1537    1812    2022    2354    2533    1537 
   Size:        275     210     332     179    4090

  Fragments arranged by size: 

                4090     332     275     210     179
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3016    1353 
   Size:       1663    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3673    4047    4193     387 
   Size:       1064     138    2084     374     146    1280

  Fragments arranged by size: 

                2084    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2234    2288    2309    3149    3746    4533    4801 
   Size:        393     602      54      21     840     597     787     268

Cuts at:   4801    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     840     787     602     597     393     268      54
                  21
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2279    2279 
   Size:       5086
 
EcoRI G'AATT_C

Cuts at:   2395    2773    2395 
   Size:        378    4708
 
FspI TGC'GCA

Cuts at:   3592    3592 
   Size:       5086
 
HaeII r_GCGC'y

Cuts at:   1678    2060    4233    1678 
   Size:        382    2173    2531

  Fragments arranged by size: 

                2531    2173     382
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3727    3759    3801    3833    1373 
   Size:         32     135      32    2155      32      42      32    2626

  Fragments arranged by size: 

                2626    2155     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2430    2676     669 
   Size:        988     281     492     246    3079

  Fragments arranged by size: 

                3079     988     492     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5086
 
HpaI GTT'AAC

Cuts at:   2676    2676 
   Size:       5086
 
KasI G'GCGC_C

Cuts at:   2056    2056 
   Size:       5086
 
KpnI G_GTAC'C

Cuts at:   2411    2411 
   Size:       5086
 
MfeI C'AATT_G

Cuts at:   2685    2685 
   Size:       5086
 
MluI A'CGCG_T

Cuts at:   4962    4962 
   Size:       5086
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2141    4200    4384    1305 
   Size:        836    2059     184    2007

  Fragments arranged by size: 

                2059    2007     836     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3264

  Fragments arranged by size: 

                3264    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3044    3403    3562     518 
   Size:        648    1878     359     159    2042

  Fragments arranged by size: 

                2042    1878     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2314    2502    3179    4327    4572    1606 
   Size:        708     188     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     708     677     245     188
 
NaeI GCC'GGC

Cuts at:   2029    2371    2029 
   Size:        342    4744
 
NarI GG'CG_CC

Cuts at:   2057    2057 
   Size:       5086
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4534
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5086
 
NgoMIV G'CCGG_C

Cuts at:   2027    2369    2027 
   Size:        342    4744
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5086
 
NotI GC'GGCC_GC

Cuts at:   2533    2533 
   Size:       5086
 
NspI r_CATG'y

Cuts at:   1132    1229    2444    3989    1132 
   Size:         97    1215    1545    2229

  Fragments arranged by size: 

                2229    1545    1215      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5086
 
PciI A'CATG_T

Cuts at:   3985    3985 
   Size:       5086
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5086
 
PmeI GTTT'AAAC

Cuts at:   2390    2390 
   Size:       5086
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3155    3187    4668    4700    3155 
   Size:         32    1481      32    3541

  Fragments arranged by size: 

                3541    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5086
 
PsiI TTA'TAA

Cuts at:   2656    2656 
   Size:       5086
 
PstI C_TGCA'G

Cuts at:    830    2043    2438     830 
   Size:       1213     395    3478

  Fragments arranged by size: 

                3478    1213     395
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5086
 
SalI G'TCGA_C

Cuts at:   1936    2428    1936 
   Size:        492    4594
 
SbfI CC_TGCA'GG

Cuts at:   2438    2438 
   Size:       5086
 
ScaI AGT'ACT

Cuts at:   3334    5046    3334 
   Size:       1712    3374
 
SfcI C'TryA_G

Cuts at:    826    1045    2039    2434    3569    4250    4441     826 
   Size:        219     994     395    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135     994     681     395     219     191
 
SfoI GGC'GCC

Cuts at:   2058    2058 
   Size:       5086
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5086
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5086
 
SmaI CCC'GGG

Cuts at:   1872    2062    2413    1872 
   Size:        190     351    4545

  Fragments arranged by size: 

                4545     351     190
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3190    4091    4353     820 
   Size:        197     926    1247     901     262    1553

  Fragments arranged by size: 

                1553    1247     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2877     493 
   Size:       2384    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5086
 
SphI G_CATG'C

Cuts at:   1132    2444    1132 
   Size:       1312    3774
 
SspI AAT'ATT

Cuts at:      5      52    3010       5 
   Size:         47    2958    2081

  Fragments arranged by size: 

                2958    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5086
 
StyI C'CwwG_G

Cuts at:    513    1065    2525     513 
   Size:        552    1460    3074

  Fragments arranged by size: 

                3074    1460     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3113    3130    3283    3468    3913     325 
   Size:       1255    1533      17     153     185     445    1498

  Fragments arranged by size: 

                1533    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3332    5044      96 
   Size:        274      80      33      51    1443    1355    1712     138

  Fragments arranged by size: 

                1712    1443    1355     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5046    5078    5046 
   Size:         32    5054
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5086
 
XbaI T'CTAG_A

Cuts at:   2422    2422 
   Size:       5086
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5086
 
XmaI C'CCGG_G

Cuts at:   1870    2060    2411    1870 
   Size:        190     351    4545

  Fragments arranged by size: 

                4545     351     190
 
XmnI GAAnn'nnTTC

Cuts at:   3215    3215 
   Size:       5086
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2894     276 
   Size:         53      83     186     936    1360    2468

  Fragments arranged by size: 

                2468    1360     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoNI    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NspI     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI
     PstI     PvuI     SalI     SbfI     ScaI     SfcI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   DraIII
  EcoICRI EcoO109I    EcoRV     FalI     FseI    FspAI     NruI     NsiI
     PacI     PfoI     PmlI    PpuMI   PspOMI    PspXI     PsrI    PvuII
    RsrII     SacI    SacII    SanDI     SapI    SexAI     SfiI     SrfI
     SwaI     XcmI
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