(Circular) (Six-base) MAPSORT of: pFN21AB7509.seq Check: 5257 from: 1 to: 5041 pFN21AB7509 5041 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2851 278 Size: 53 83 186 936 1315 2468 Fragments arranged by size: 2468 1315 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2384 1937 Size: 447 4594 Acc65I G'GTAC_C Cuts at: 2362 2362 Size: 5041 AclI AA'CG_TT Cuts at: 1563 3168 3541 1563 Size: 1605 373 3063 Fragments arranged by size: 3063 1605 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3104 4488 4756 1632 Size: 1472 1384 268 1917 Fragments arranged by size: 1917 1472 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4844 AflIII A'CryG_T Cuts at: 1203 2206 3940 4917 1203 Size: 1003 1734 977 1327 Fragments arranged by size: 1734 1327 1003 977 AgeI A'CCGG_T Cuts at: 3872 3872 Size: 5041 AhdI GACnn_n'nnGTC Cuts at: 3770 3770 Size: 5041 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5041 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5041 AlwNI CAG_nnn'CTG Cuts at: 2784 4356 2784 Size: 1572 3469 ApaLI G'TGCA_C Cuts at: 3098 4254 3098 Size: 1156 3885 ApoI r'AATT_y Cuts at: 1493 2087 2279 2350 2576 2728 4837 1493 Size: 594 192 71 226 152 2109 1697 Fragments arranged by size: 2109 1697 594 226 192 152 71 AseI AT'TA_AT Cuts at: 160 1032 3595 160 Size: 872 2563 1606 Fragments arranged by size: 2563 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5041 AvaI C'yCGr_G Cuts at: 1870 1943 2366 1870 Size: 73 423 4545 Fragments arranged by size: 4545 423 73 BamHI G'GATC_C Cuts at: 2371 2738 2371 Size: 367 4674 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2362 3817 618 Size: 325 217 627 575 1455 1842 Fragments arranged by size: 1842 1455 627 575 325 217 BanII G_rGCy'C Cuts at: 1391 2360 1391 Size: 969 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4070 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3232 3266 1903 Size: 34 1295 34 3678 Fragments arranged by size: 3678 1295 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2933 4143 2933 Size: 1210 3831 BclI T'GATC_A Cuts at: 1548 1548 Size: 5041 BfrBI ATG'CAT Cuts at: 2111 2226 2111 Size: 115 4926 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3652 136 Size: 107 122 71 631 605 1980 1525 Fragments arranged by size: 1980 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5036 5036 Size: 5041 BlpI GC'TnA_GC Cuts at: 2458 2458 Size: 5041 Bme1580I G_kGCm'C Cuts at: 1790 3102 4258 1790 Size: 1312 1156 2573 Fragments arranged by size: 2573 1312 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5041 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2772 3730 442 Size: 416 53 83 399 24 1355 958 1753 Fragments arranged by size: 1753 1355 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5041 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3701 1239 Size: 2462 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3166 4031 4329 3166 Size: 865 298 3878 Fragments arranged by size: 3878 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3704 882 Size: 617 2205 2219 Fragments arranged by size: 2219 2205 617 BsaAI yAC'GTr Cuts at: 493 1204 2238 2832 493 Size: 711 1034 594 2702 Fragments arranged by size: 2702 1034 711 594 BsaBI GATnn'nnATC Cuts at: 5035 5035 Size: 5041 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2848 3230 3865 275 Size: 53 83 186 936 1315 382 635 1451 Fragments arranged by size: 1451 1315 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3474 3872 4146 4293 4704 3474 Size: 398 274 147 411 3811 Fragments arranged by size: 3811 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4816 Fragments arranged by size: 4816 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 1186 Size: 844 4197 BseYI C'CCAG_C Cuts at: 4244 4740 4244 Size: 496 4545 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4468 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2491 3252 4280 1540 Size: 275 186 490 761 1028 2301 Fragments arranged by size: 2301 1028 761 490 275 186 BsiHKAI G_wGCw'C Cuts at: 3102 3187 4258 3102 Size: 85 1071 3885 Fragments arranged by size: 3885 1071 85 BsmI GAATG_Cn' Cuts at: 2552 2645 2552 Size: 93 4948 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2360 3102 3187 4258 1391 Size: 399 570 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 570 399 85 BspEI T'CCGG_A Cuts at: 4704 4704 Size: 5041 BspHI T'CATG_A Cuts at: 2930 4660 2930 Size: 1730 3311 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2396 844 Size: 1552 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2928 1394 Size: 429 1105 3507 Fragments arranged by size: 3507 1105 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3536 3710 65 Size: 1183 2288 174 1396 Fragments arranged by size: 2288 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3685 3872 1700 Size: 110 1875 187 2869 Fragments arranged by size: 2869 1875 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5041 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5041 BssSI C'ACGA_G Cuts at: 3101 4113 3101 Size: 1012 4029 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5041 BstBI TT'CG_AA Cuts at: 3845 3845 Size: 5041 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5041 BstYI r'GATC_y Cuts at: 1972 2371 2738 3123 3140 4592 4678 4690 5036 Size: 399 367 385 17 1452 86 12 346 Cuts at: 5036 1972 Size: 1977 Fragments arranged by size: 1977 1452 399 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3550 Fragments arranged by size: 3550 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4302 Fragments arranged by size: 4302 683 56 BtsI GCAGTG_nn' Cuts at: 2557 3351 3371 2557 Size: 794 20 4227 Fragments arranged by size: 4227 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5041 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5006 DraI TTT'AAA Cuts at: 2345 2345 Size: 5041 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4048 809 Size: 507 2732 1802 Fragments arranged by size: 2732 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2080 2488 3377 Size: 54 1425 50 275 18 250 408 889 Cuts at: 3377 5006 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 408 275 250 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2488 1537 Size: 275 676 4090 Fragments arranged by size: 4090 676 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2971 1353 Size: 1618 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3628 4002 4148 387 Size: 1064 138 2039 374 146 1280 Fragments arranged by size: 2039 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3104 3701 4488 4756 1239 Size: 393 1472 597 787 268 1524 Fragments arranged by size: 1524 1472 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2042 2042 Size: 5041 EcoRI G'AATT_C Cuts at: 2279 2350 2728 2279 Size: 71 378 4592 Fragments arranged by size: 4592 378 71 EcoRV GAT'ATC Cuts at: 2070 2070 Size: 5041 FspI TGC'GCA Cuts at: 3547 3547 Size: 5041 HaeII r_GCGC'y Cuts at: 1678 4188 1678 Size: 2510 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3682 3714 3756 3788 1373 Size: 32 135 32 2110 32 42 32 2626 Fragments arranged by size: 2626 2110 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2385 2631 669 Size: 988 281 447 246 3079 Fragments arranged by size: 3079 988 447 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5041 HpaI GTT'AAC Cuts at: 2631 2631 Size: 5041 KpnI G_GTAC'C Cuts at: 2366 2366 Size: 5041 MfeI C'AATT_G Cuts at: 2286 2640 2286 Size: 354 4687 MluI A'CGCG_T Cuts at: 4917 4917 Size: 5041 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4155 4339 1305 Size: 2850 184 2007 Fragments arranged by size: 2850 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2082 10 Size: 54 1425 343 250 2969 Fragments arranged by size: 2969 1425 343 250 54 MslI CAynn'nnrTG Cuts at: 518 1166 2999 3358 3517 518 Size: 648 1833 359 159 2042 Fragments arranged by size: 2042 1833 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2457 3134 4282 4527 1606 Size: 851 677 1148 245 2120 Fragments arranged by size: 2120 1148 851 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3550 Fragments arranged by size: 3550 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5041 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5041 NotI GC'GGCC_GC Cuts at: 2488 2488 Size: 5041 NsiI A_TGCA'T Cuts at: 2113 2228 2113 Size: 115 4926 NspI r_CATG'y Cuts at: 1132 1229 2111 2210 2230 2399 3944 1132 Size: 97 882 99 20 169 1545 2229 Fragments arranged by size: 2229 1545 882 169 99 97 20 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5041 PciI A'CATG_T Cuts at: 2206 3940 2206 Size: 1734 3307 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5041 PmeI GTTT'AAAC Cuts at: 2345 2345 Size: 5041 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3110 3142 4623 4655 3110 Size: 32 1481 32 3496 Fragments arranged by size: 3496 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2042 2042 Size: 5041 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5041 PsiI TTA'TAA Cuts at: 2611 2611 Size: 5041 PstI C_TGCA'G Cuts at: 830 2393 830 Size: 1563 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5041 SalI G'TCGA_C Cuts at: 1936 2383 1936 Size: 447 4594 SbfI CC_TGCA'GG Cuts at: 2393 2393 Size: 5041 ScaI AGT'ACT Cuts at: 3289 5001 3289 Size: 1712 3329 SfcI C'TryA_G Cuts at: 826 1045 2389 3524 4205 4396 826 Size: 219 1344 1135 681 191 1471 Fragments arranged by size: 1471 1344 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5041 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5041 SmaI CCC'GGG Cuts at: 1872 2368 1872 Size: 496 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3145 4046 4308 820 Size: 197 926 1202 901 262 1553 Fragments arranged by size: 1553 1202 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2238 2832 493 Size: 1745 594 2702 Fragments arranged by size: 2702 1745 594 SpeI A'CTAG_T Cuts at: 152 152 Size: 5041 SphI G_CATG'C Cuts at: 1132 2399 1132 Size: 1267 3774 SspI AAT'ATT Cuts at: 5 52 2965 5 Size: 47 2913 2081 Fragments arranged by size: 2913 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5041 StyI C'CwwG_G Cuts at: 513 1065 2004 2480 513 Size: 552 939 476 3074 Fragments arranged by size: 3074 939 552 476 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3068 3085 3238 3423 3868 325 Size: 1255 1488 17 153 185 445 1498 Fragments arranged by size: 1498 1488 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3287 4999 96 Size: 274 80 33 51 1443 1310 1712 138 Fragments arranged by size: 1712 1443 1310 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2257 2289 5001 5033 2257 Size: 32 2712 32 2265 Fragments arranged by size: 2712 2265 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5041 XbaI T'CTAG_A Cuts at: 2377 2377 Size: 5041 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5041 XmaI C'CCGG_G Cuts at: 1870 2366 1870 Size: 496 4545 XmnI GAAnn'nnTTC Cuts at: 3170 3170 Size: 5041 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2849 276 Size: 53 83 186 936 1315 2468 Fragments arranged by size: 2468 1315 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI