Restriction map for pFN21AB7452
 (Linear) (Six-base) MAPSORT of: KIBB7452.seq  Check: 5306  from: 1  to: 813



KIBB7452.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     568     813 
   Size:        568     245
 
Acc65I G'GTAC_C

Cuts at:      0      30     813 
   Size:         30     783
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     813 
   Size:        178     635
 
AlwNI CAG_nnn'CTG

Cuts at:      0     273     291     612     813 
   Size:        273      18     321     201

  Fragments arranged by size: 

                 321     273     201      18
 
AvaI C'yCGr_G

Cuts at:      0      77     178     261     469     813 
   Size:         77     101      83     208     344

  Fragments arranged by size: 

                 344     208     101      83      77
 
BanI G'GyrC_C

Cuts at:      0      30     247     308     483     525     741     813 
   Size:         30     217      61     175      42     216      72

  Fragments arranged by size: 

                 217     216     175      72      61      42      30
 
BanII G_rGCy'C

Cuts at:      0     260     813 
   Size:        260     553
 
BbeI G_GCGC'C

Cuts at:      0     251     487     745     813 
   Size:        251     236     258      68

  Fragments arranged by size: 

                 258     251     236      68
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     531     813 
   Size:        531     282
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      84     813 
   Size:         84     729
 
BglI GCCn_nnn'nGGC

Cuts at:      0     199     458     634     650     813 
   Size:        199     259     176      16     163

  Fragments arranged by size: 

                 259     199     176     163      16
 
BlpI GC'TnA_GC

Cuts at:      0     532     813 
   Size:        532     281
 
Bme1580I G_kGCm'C

Cuts at:      0     553     813 
   Size:        553     260
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     747     813 
   Size:        747      66
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     595     634     694     742     787     813 
   Size:        187     408      39      60      48      45      26

  Fragments arranged by size: 

                 408     187      60      48      45      39      26
 
Bpu10I CC'TnA_GC

Cuts at:      0     379     439     813 
   Size:        379      60     374

  Fragments arranged by size: 

                 379     374      60
 
BsaAI yAC'GTr

Cuts at:      0     145     813 
   Size:        145     668
 
BsaHI Gr'CG_yC

Cuts at:      0     248     484     698     742     813 
   Size:        248     236     214      44      71

  Fragments arranged by size: 

                 248     236     214      71      44
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     118     196     487     772     813 
   Size:        118      78     291     285      41

  Fragments arranged by size: 

                 291     285     118      78      41
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     523     813 
   Size:        523     290
 
BsiEI CG_ry'CG

Cuts at:      0      44      63      95     191     626     642     813 
   Size:         44      19      32      96     435      16     171

  Fragments arranged by size: 

                 435     171      96      44      32      19      16
 
Bsp1286I G_dGCh'C

Cuts at:      0     260     553     813 
   Size:        260     293     260

  Fragments arranged by size: 

                 293     260     260
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     111     568     813 
   Size:        111     457     245

  Fragments arranged by size: 

                 457     245     111
 
BsrFI r'CCGG_y

Cuts at:      0      39      43     385     592     727     804     813 
   Size:         39       4     342     207     135      77       9

  Fragments arranged by size: 

                 342     207     135      77      39       9       4
 
BssHII G'CGCG_C

Cuts at:      0     299     586     588     596     680     712     813 
   Size:        299     287       2       8      84      32     101

  Fragments arranged by size: 

                 299     287     101      84      32       8       2
 
BssSI C'ACGA_G

Cuts at:      0     517     813 
   Size:        517     296
 
BtgI C'CryG_G

Cuts at:      0     632     813 
   Size:        632     181
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     774     813 
   Size:        774      39
 
BtsI GCAGTG_nn'

Cuts at:      0      14     511     813 
   Size:         14     497     302

  Fragments arranged by size: 

                 497     302      14
 
EaeI y'GGCC_r

Cuts at:      0      41      60      92     459     623     635     813 
   Size:         41      19      32     367     164      12     178

  Fragments arranged by size: 

                 367     178     164      41      32      19      12
 
EagI C'GGCC_G

Cuts at:      0      41      60      92     623     813 
   Size:         41      19      32     531     190

  Fragments arranged by size: 

                 531     190      41      32      19
 
EarI CTCTTCn'nnn_

Cuts at:      0     394     607     790     813 
   Size:        394     213     183      23

  Fragments arranged by size: 

                 394     213     183      23
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178     187     595     634     694     742     787     813 
   Size:        178       9     408      39      60      48      45      26

  Fragments arranged by size: 

                 408     178      60      48      45      39      26       9
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     118     157     813 
   Size:        118      39     656

  Fragments arranged by size: 

                 656     118      39
 
FspI TGC'GCA

Cuts at:      0     496     813 
   Size:        496     317
 
HaeII r_GCGC'y

Cuts at:      0     251     291     386     487     745     813 
   Size:        251      40      95     101     258      68

  Fragments arranged by size: 

                 258     251     101      95      68      40
 
KasI G'GCGC_C

Cuts at:      0     247     483     741     813 
   Size:        247     236     258      72

  Fragments arranged by size: 

                 258     247     236      72
 
KpnI G_GTAC'C

Cuts at:      0      34     813 
   Size:         34     779
 
MspA1I CmG'CkG

Cuts at:      0      59     489     531     609     813 
   Size:         59     430      42      78     204

  Fragments arranged by size: 

                 430     204      78      59      42
 
NaeI GCC'GGC

Cuts at:      0      41     387     594     729     813 
   Size:         41     346     207     135      84

  Fragments arranged by size: 

                 346     207     135      84      41
 
NarI GG'CG_CC

Cuts at:      0     248     484     742     813 
   Size:        248     236     258      71

  Fragments arranged by size: 

                 258     248     236      71
 
NgoMIV G'CCGG_C

Cuts at:      0      39     385     592     727     813 
   Size:         39     346     207     135      86

  Fragments arranged by size: 

                 346     207     135      86      39
 
NotI GC'GGCC_GC

Cuts at:      0      60     813 
   Size:         60     753
 
NruI TCG'CGA

Cuts at:      0     793     813 
   Size:        793      20
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     612     813 
   Size:        612     201
 
PfoI T'CCnGG_A

Cuts at:      0     719     813 
   Size:        719      94
 
PstI C_TGCA'G

Cuts at:      0     281     689     813 
   Size:        281     408     124

  Fragments arranged by size: 

                 408     281     124
 
PvuI CG_AT'CG

Cuts at:      0     191     813 
   Size:        191     622
 
PvuII CAG'CTG

Cuts at:      0     609     813 
   Size:        609     204
 
SapI GCTCTTCn'nnn_

Cuts at:      0     607     790     813 
   Size:        607     183      23

  Fragments arranged by size: 

                 607     183      23
 
SfcI C'TryA_G

Cuts at:      0     277     426     685     813 
   Size:        277     149     259     128

  Fragments arranged by size: 

                 277     259     149     128
 
SfoI GGC'GCC

Cuts at:      0     249     485     743     813 
   Size:        249     236     258      70

  Fragments arranged by size: 

                 258     249     236      70
 
SgrAI Cr'CCGG_yG

Cuts at:      0     385     592     813 
   Size:        385     207     221

  Fragments arranged by size: 

                 385     221     207
 
SmaI CCC'GGG

Cuts at:      0      79     263     813 
   Size:         79     184     550

  Fragments arranged by size: 

                 550     184      79
 
XmaI C'CCGG_G

Cuts at:      0      77     261     813 
   Size:         77     184     552

  Fragments arranged by size: 

                 552     184      77
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI    AlwNI     AvaI     BanI    BanII     BbeI
     BbsI    BciVI     BglI     BlpI Bme1580I     BplI     BpmI   Bpu10I
    BsaAI    BsaHI    BseRI     BsgI    BsiEI Bsp1286I    BspMI    BsrFI
   BssHII    BssSI     BtgI    BtgZI     BtsI     EaeI     EagI     EarI
  Eco57MI    EcoNI     FspI    HaeII     KasI     KpnI   MspA1I     NaeI
     NarI   NgoMIV     NotI     NruI    PflMI     PfoI     PstI     PvuI
    PvuII     SapI     SfcI     SfoI    SgrAI     SmaI     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI     BclI    BfrBI
    BglII    BmgBI     BmrI     BmtI    BpuEI     BsaI    BsaBI    BsaWI
    BsaXI    BseYI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrGI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI     EciI
  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI    FspAI    Hin4I
   HincII  HindIII     HpaI     MfeI     MluI     MmeI     MscI     MslI
     NcoI     NdeI     NheI     NsiI     NspI     PacI     PasI     PciI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SgfI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI
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