Restriction map for
pFN21AB7441
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# NO CUT ENZYMES ( 168 enzymes) #
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Acc65I AccII AccIII AclI AcsI
AflII AgeI AhdI AlwNI AocI
Aor51HI ApaI ApoI AscI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaBI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FseI
FspI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HindIII
HpaI HpaII HphI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tsp509I Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 595 GACGTC GACGT^C
AccI 172 GTAGAC GT^[AC][GT]AC
AcyI 595 GACGTC G[GA]^CG[CT]C
AfaI 149 GTAC GT^AC
AflIII 54 ACATGT A^C[GA][CT]GT
AlwI 387 GGATC GGATC(4/5)
AseI 613 ATTAAT AT^TAAT
AsnI 613 ATTAAT AT^TAAT
AvaII 558 GGTCC G^G[AT]CC
BclI 546 TGATCA T^GATCA
BfaI 604 CTAG C^TAG
BglII 28 AGATCT A^GATCT
BpmI 553 CTGGAG CTGGAG(16/14)
BsaAI 367 TACGTG [CT]AC^GT[GA]
BsaHI 595 GACGTC G[GA]^CG[CT]C
BsaI 184 GGTCTC GGTCTC(1/5)
BsiYI 258 CCAAGCTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 258 CCAAGCTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspHI 624 TCATGA T^CATGA
BspLU11I 54 ACATGT A^CATGT
BsrI 85 CCAGT ACTGG(1/-1)
BstXI 85 CCAGTGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 28 AGATCT [GA]^GATC[CT]
Cac8I 500 GCAGGC GC[ACGT]^[ACGT]GC
Cfr13I 558 GGTCC G^G[ACGT]CC
EarI 226 GAAGAG CTCTTC(1/4)
FbaI 546 TGATCA T^GATCA
Hin1I 595 GACGTC G[GA]^CG[CT]C
Ksp632I 226 GAAGAG CTCTTC(1/4)
MaeI 604 CTAG C^TAG
MboII 226 GAAGA GAAGA(8/7)
MflI 28 AGATCT [GA]^GATC[CT]
MspA1I 550 CAGCTG C[AC]G^C[GT]G
PshBI 613 ATTAAT AT^TAAT
PvuII 550 CAGCTG CAG^CTG
RcaI 624 TCATGA T^CATGA
RsaI 149 GTAC GT^AC
SacI 589 GAGCTC GAGCT^C
Sau96I 558 GGTCC G^G[ACGT]CC
SmlI 414 CTTGAG C^T[CT][GA]AG
TaqI 593 TCGA T^CGA
TfiI 621 GAATC G^A[AT]TC
TthHB8I 593 TCGA T^CGA
XhoII 28 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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AciI 22 CCGC CCGC(-3/-1)
468 GCGG
Alw44I 3 GTGCAC G^TGCAC
58 GTGCAC
ApaLI 3 GTGCAC G^TGCAC
58 GTGCAC
BanII 96 GGGCTC G[GA]GC[CT]^C
589 GAGCTC
BsmAI 304 GAGAC GTCTC(1/5)
185 GTCTC
BsrDI 432 CATTGC GCAATG(2/0)
471 GCAATG
BstF5I 255 CATCC GGATG(2/0)
531 CATCC
DsaI 106 CCACGG C^C[GA][CT]GG
180 CCACGG
FokI 255 CATCC GGATG(9/13)
531 CATCC
MaeII 368 ACGT A^CGT
596 ACGT
MwoI 253 GCCATCCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
511 GCCACCAATGC
NlaIV 140 GGCTCC GG[ACGT]^[ACGT]CC
396 GGAGCC
PleI 306 GACTC GAGTC(4/5)
527 GAGTC
PstI 310 CTGCAG CTGCA^G
498 CTGCAG
SfcI 310 CTGCAG C^T[GA][CT]AG
498 CTGCAG
XcmI 347 CCAAGAACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
572 CCATTAAATCCCTGG
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AluI AspHI BbvI BmyI BsaJI
BsiHKAI Bsp1286I BssKI BstNI DdeI
DpnII EcoRII Fnu4HI HinfI ItaI
MaeIII MboI MnlI MseI MslI
MvaI NdeII NlaIII NspI Sau3AI
ScrFI Tru9I TseI Tsp45I TspRI