Restriction map for
pFN21AB7417
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BanII BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Cac8I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HgaI
Hin1I HindIII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfaNI SfiI SfoI SfuI SgrAI
SpeI SphI Sse8387I SspBI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AocI 447 CCTGAGG CC^T[ACGT]AGG
AvaI 1020 CCCGGG C^[CT]CG[GA]G
BanI 517 GGCACC G^G[CT][GA]CC
BbsI 679 GTCTTC GAAGAC(2/6)
BciVI 26 GGATAC GTATCC(6/5)
BmyI 418 GGGCAC G[AGT]GC[ACT]^C
BpuAI 679 GTCTTC GAAGAC(2/6)
BsaI 743 GAGACC GGTCTC(1/5)
BsmFI 407 GGGAC GGGAC(10/14)
BsoBI 1020 CCCGGG C^[CT]CG[GA]G
Bsp1286I 418 GGGCAC G[AGT]GC[ACT]^C
BspMI 481 GCAGGT ACCTGC(4/8)
BsrDI 1003 CATTGC GCAATG(2/0)
BstAPI 1007 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1053 CCACGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 281 AGATCC [GA]^GATC[CT]
Bsu36I 447 CCTGAGG CC^T[ACGT]AGG
CfoI 1080 GCGC GCG^C
Eco81I 447 CCTGAGG CC^T[ACGT]AGG
EcoNI 393 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 1079 AGCGCC [GA]GCGC^[CT]
HhaI 1080 GCGC GCG^C
HinP1I 1080 GCGC G^CGC
HincII 75 GTTGAC GT[CT]^[GA]AC
HindII 75 GTTGAC GT[CT]^[GA]AC
MflI 281 AGATCC [GA]^GATC[CT]
MseI 88 TTAA T^TAA
MspA1I 382 CAGCTG C[AC]G^C[GT]G
NdeI 778 CATATG CA^TATG
PflMI 726 CCAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1042 GACTC GAGTC(4/5)
PstI 369 CTGCAG CTGCA^G
PvuII 382 CAGCTG CAG^CTG
SexAI 524 ACCTGGT A^CC[AT]GGT
SfcI 369 CTGCAG C^T[GA][CT]AG
SmaI 1020 CCCGGG CCC^GGG
SmlI 356 CTCAAG C^T[CT][GA]AG
SnaBI 363 TACGTA TAC^GTA
TfiI 1033 GATTC G^A[AT]TC
Tru9I 88 TTAA T^TAA
Van91I 726 CCAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 467 CCACAGCACTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 281 AGATCC [GA]^GATC[CT]
XmaCI 1020 CCCGGG C^CCGGG
XmaI 1020 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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Asp700I 174 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
821 GAAATATTTC
AvaII 408 GGACC G^G[AT]CC
866 GGTCC
BbvI 1077 GCAGC GCAGC(8/12)
573 GCTGC
BfaI 271 CTAG C^TAG
812 CTAG
BsaAI 363 TACGTA [CT]AC^GT[GA]
1054 CACGTA
BsgI 981 GTGCAG GTGCAG(16/14)
1075 GTGCAG
EcoT14I 168 CCAAGG C^C[AT][AT]GG
624 CCAAGG
Fnu4HI 573 GCTGC GC^[ACGT]GC
1077 GCAGC
HapII 925 CCGG C^CGG
1021 CCGG
HinfI 1033 GATTC G^A[ACGT]TC
1042 GACTC
HpaII 925 CCGG C^CGG
1021 CCGG
ItaI 573 GCTGC GC^[ACGT]GC
1077 GCAGC
MaeI 271 CTAG C^TAG
812 CTAG
MaeII 364 ACGT A^CGT
1055 ACGT
MaeIII 499 GTGAC ^GT[ACGT]AC
966 GTCAC
MspI 925 CCGG C^CGG
1021 CCGG
SspI 188 AATATT AAT^ATT
823 AATATT
StuI 769 AGGCCT AGG^CCT
791 AGGCCT
StyI 168 CCAAGG C^C[AT][AT]GG
624 CCAAGG
TaqI 57 TCGA T^CGA
948 TCGA
TseI 573 GCTGC G^C[AT]GC
1077 GCAGC
Tsp45I 499 GTGAC ^GT[GC]AC
966 GTCAC
Tsp509I 62 AATT ^AATT
90 AATT
TthHB8I 57 TCGA T^CGA
948 TCGA
XmnI 174 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
821 GAAATATTTC
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AfaI AluI AlwI BcnI BpmI
BsaJI BsiYI BslI BsmAI BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EcoRII FokI HaeIII HphI
MboI MboII MnlI MslI MvaI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI TspRI