(Circular) (Six-base) MAPSORT of: pFN21AB7417.seq Check: 9047 from: 1 to: 5800 pFN21AB7417 5800 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2477 2477 Size: 5800 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3610 278 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3143 1937 Size: 1206 4594 Acc65I G'GTAC_C Cuts at: 3121 3121 Size: 5800 AclI AA'CG_TT Cuts at: 1563 3927 4300 1563 Size: 2364 373 3063 Fragments arranged by size: 3063 2364 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2478 3863 5247 5515 1632 Size: 846 1385 1384 268 1917 Fragments arranged by size: 1917 1385 1384 846 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5603 AflIII A'CryG_T Cuts at: 1203 4699 5676 1203 Size: 3496 977 1327 Fragments arranged by size: 3496 1327 977 AgeI A'CCGG_T Cuts at: 4631 4631 Size: 5800 AhdI GACnn_n'nnGTC Cuts at: 4529 4529 Size: 5800 AleI CACnn'nnGTG Cuts at: 1166 2215 2309 2592 3077 1166 Size: 1049 94 283 485 3889 Fragments arranged by size: 3889 1049 485 283 94 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5800 AlwNI CAG_nnn'CTG Cuts at: 3543 5115 3543 Size: 1572 4228 ApaLI G'TGCA_C Cuts at: 3857 5013 3857 Size: 1156 4644 ApoI r'AATT_y Cuts at: 1493 3109 3335 3487 5596 1493 Size: 1616 226 152 2109 1697 Fragments arranged by size: 2109 1697 1616 226 152 AseI AT'TA_AT Cuts at: 160 1032 4354 160 Size: 872 3322 1606 Fragments arranged by size: 3322 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 AvaI C'yCGr_G Cuts at: 1870 1943 3025 3125 1870 Size: 73 1082 100 4545 Fragments arranged by size: 4545 1082 100 73 BamHI G'GATC_C Cuts at: 3130 3497 3130 Size: 367 5433 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2522 3121 4576 618 Size: 325 217 627 735 599 1455 1842 Fragments arranged by size: 1842 1455 735 627 599 325 217 BanII G_rGCy'C Cuts at: 1391 3119 1391 Size: 1728 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2677 928 Size: 971 778 4051 Fragments arranged by size: 4051 971 778 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3991 4025 1903 Size: 34 2054 34 3678 Fragments arranged by size: 3678 2054 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2025 3692 4902 2025 Size: 1667 1210 2923 Fragments arranged by size: 2923 1667 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5800 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4411 136 Size: 107 122 71 631 605 2739 1525 Fragments arranged by size: 2739 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5795 5795 Size: 5800 BlpI GC'TnA_GC Cuts at: 3217 3217 Size: 5800 Bme1580I G_kGCm'C Cuts at: 1790 2427 3861 5017 1790 Size: 637 1434 1156 2573 Fragments arranged by size: 2573 1434 1156 637 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5800 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2586 3531 4489 Size: 416 53 83 399 24 1169 945 958 Cuts at: 4489 442 Size: 1753 Fragments arranged by size: 1753 1169 958 945 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5800 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2978 2978 Size: 5800 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2423 2635 3002 4460 1239 Size: 1184 212 367 1458 2579 Fragments arranged by size: 2579 1458 1184 367 212 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2346 3925 4790 5088 2346 Size: 1579 865 298 3058 Fragments arranged by size: 3058 1579 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2742 4463 882 Size: 617 1243 1721 2219 Fragments arranged by size: 2219 1721 1243 617 BsaAI yAC'GTr Cuts at: 493 1204 2370 3061 3591 493 Size: 711 1166 691 530 2702 Fragments arranged by size: 2702 1166 711 691 530 BsaBI GATnn'nnATC Cuts at: 5794 5794 Size: 5800 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3607 3989 4624 275 Size: 53 83 186 936 2074 382 635 1451 Fragments arranged by size: 2074 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4233 4631 4905 5052 5463 4233 Size: 398 274 147 411 4570 Fragments arranged by size: 4570 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2660 2690 3038 3068 1178 Size: 30 165 30 1257 30 348 30 3910 Fragments arranged by size: 3910 1257 348 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5800 BseYI C'CCAG_C Cuts at: 3065 5003 5499 3065 Size: 1938 496 3366 Fragments arranged by size: 3366 1938 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3007 3101 1163 Size: 573 1271 94 3862 Fragments arranged by size: 3862 1271 573 94 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3250 4011 5039 1540 Size: 275 186 1249 761 1028 2301 Fragments arranged by size: 2301 1249 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3861 3946 5017 3861 Size: 85 1071 4644 Fragments arranged by size: 4644 1071 85 BsmI GAATG_Cn' Cuts at: 3311 3404 3311 Size: 93 5707 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2427 3119 3861 3946 5017 1391 Size: 399 637 692 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 692 637 399 85 BspEI T'CCGG_A Cuts at: 5463 5463 Size: 5800 BspHI T'CATG_A Cuts at: 3689 5419 3689 Size: 1730 4070 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2477 3155 844 Size: 1633 678 3489 Fragments arranged by size: 3489 1633 678 BsrBI CCG'CTC Cuts at: 1394 1823 3687 1394 Size: 429 1864 3507 Fragments arranged by size: 3507 1864 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3007 4295 4469 65 Size: 1183 1759 1288 174 1396 Fragments arranged by size: 1759 1396 1288 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4444 4631 1700 Size: 110 2634 187 2869 Fragments arranged by size: 2869 2634 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5800 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5800 BssSI C'ACGA_G Cuts at: 3860 4872 3860 Size: 1012 4788 BstAPI GCAn_nnn'nTGC Cuts at: 794 3018 794 Size: 2224 3576 BstBI TT'CG_AA Cuts at: 4604 4604 Size: 5800 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5800 BstXI CCAn_nnnn'nTGG Cuts at: 3065 3065 Size: 5800 BstYI r'GATC_y Cuts at: 1972 2286 3130 3497 3882 3899 5351 5437 5449 Size: 314 844 367 385 17 1452 86 12 Cuts at: 5449 5795 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 844 385 367 346 314 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2453 2453 Size: 5800 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4309 Fragments arranged by size: 4309 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5061 Fragments arranged by size: 5061 683 56 BtsI GCAGTG_nn' Cuts at: 2995 3316 4110 4130 2995 Size: 321 794 20 4665 Fragments arranged by size: 4665 794 321 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5800 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5765 DraI TTT'AAA Cuts at: 3104 3104 Size: 5800 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4807 809 Size: 507 3491 1802 Fragments arranged by size: 3491 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3247 4136 5765 Size: 54 1425 50 275 18 1417 889 1629 Cuts at: 5765 8 Size: 43 Fragments arranged by size: 1629 1425 1417 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3247 1537 Size: 275 1435 4090 Fragments arranged by size: 4090 1435 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3730 1353 Size: 2377 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4387 4761 4907 387 Size: 1064 138 2798 374 146 1280 Fragments arranged by size: 2798 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2423 2478 2635 3002 3863 4460 5247 Size: 393 791 55 157 367 861 597 787 Cuts at: 5247 5515 1239 Size: 268 1524 Fragments arranged by size: 1524 861 791 787 597 393 367 268 157 55 EcoNI CCTnn'n_nnAGG Cuts at: 2402 2402 Size: 5800 EcoRI G'AATT_C Cuts at: 3109 3487 3109 Size: 378 5422 FspI TGC'GCA Cuts at: 4306 4306 Size: 5800 HaeII r_GCGC'y Cuts at: 1678 3088 4947 1678 Size: 1410 1859 2531 Fragments arranged by size: 2531 1859 1410 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2989 3021 3038 3070 4441 Size: 32 135 32 1417 32 17 32 1371 Cuts at: 4441 4473 4515 4547 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1417 1371 135 42 32 32 32 32 32 32 17 HincII GTy'rAC Cuts at: 669 1657 1938 2082 3144 3390 669 Size: 988 281 144 1062 246 3079 Fragments arranged by size: 3079 1062 988 281 246 144 HindIII A'AGCT_T Cuts at: 748 748 Size: 5800 HpaI GTT'AAC Cuts at: 3390 3390 Size: 5800 KpnI G_GTAC'C Cuts at: 3125 3125 Size: 5800 MfeI C'AATT_G Cuts at: 3399 3399 Size: 5800 MluI A'CGCG_T Cuts at: 5676 5676 Size: 5800 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2898 4914 5098 1305 Size: 1593 2016 184 2007 Fragments arranged by size: 2016 2007 1593 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3978 Fragments arranged by size: 3978 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2215 2309 2592 3077 3758 4117 4276 Size: 648 1049 94 283 485 681 359 159 Cuts at: 4276 518 Size: 2042 Fragments arranged by size: 2042 1049 681 648 485 359 283 159 94 MspA1I CmG'CkG Cuts at: 1606 2389 3216 3893 5041 5286 1606 Size: 783 827 677 1148 245 2120 Fragments arranged by size: 2120 1148 827 783 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4309 Fragments arranged by size: 4309 939 552 NdeI CA'TA_TG Cuts at: 387 2784 387 Size: 2397 3403 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5800 NotI GC'GGCC_GC Cuts at: 3247 3247 Size: 5800 NspI r_CATG'y Cuts at: 1132 1229 3158 4703 1132 Size: 97 1929 1545 2229 Fragments arranged by size: 2229 1929 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5800 PciI A'CATG_T Cuts at: 4699 4699 Size: 5800 PflMI CCAn_nnn'nTGG Cuts at: 1108 2737 1108 Size: 1629 4171 PmeI GTTT'AAAC Cuts at: 3104 3104 Size: 5800 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3869 3901 5382 5414 3869 Size: 32 1481 32 4255 Fragments arranged by size: 4255 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5800 PsiI TTA'TAA Cuts at: 3370 3370 Size: 5800 PstI C_TGCA'G Cuts at: 830 2378 3152 830 Size: 1548 774 3478 Fragments arranged by size: 3478 1548 774 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5800 PvuII CAG'CTG Cuts at: 2389 2389 Size: 5800 SalI G'TCGA_C Cuts at: 1936 3142 1936 Size: 1206 4594 SbfI CC_TGCA'GG Cuts at: 3152 3152 Size: 5800 ScaI AGT'ACT Cuts at: 4048 5760 4048 Size: 1712 4088 SexAI A'CCwGG_T Cuts at: 2529 2529 Size: 5800 SfcI C'TryA_G Cuts at: 826 1045 2374 3148 4283 4964 5155 826 Size: 219 1329 774 1135 681 191 1471 Fragments arranged by size: 1471 1329 1135 774 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5800 SmaI CCC'GGG Cuts at: 1872 3027 3127 1872 Size: 1155 100 4545 Fragments arranged by size: 4545 1155 100 SmlI C'TyrA_G Cuts at: 820 1017 1943 2361 3904 4805 5067 820 Size: 197 926 418 1543 901 262 1553 Fragments arranged by size: 1553 1543 926 901 418 262 197 SnaBI TAC'GTA Cuts at: 493 2370 3591 493 Size: 1877 1221 2702 Fragments arranged by size: 2702 1877 1221 SpeI A'CTAG_T Cuts at: 152 152 Size: 5800 SphI G_CATG'C Cuts at: 1132 3158 1132 Size: 2026 3774 SspI AAT'ATT Cuts at: 5 52 2195 2830 3724 5 Size: 47 2143 635 894 2081 Fragments arranged by size: 2143 2081 894 635 47 StuI AGG'CCT Cuts at: 1517 2776 2798 1517 Size: 1259 22 4519 Fragments arranged by size: 4519 1259 22 StyI C'CwwG_G Cuts at: 513 1065 2004 2173 2629 3239 513 Size: 552 939 169 456 610 3074 Fragments arranged by size: 3074 939 610 552 456 169 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2308 2321 3827 3844 3997 4182 4627 Size: 1255 728 13 1506 17 153 185 445 Cuts at: 4627 325 Size: 1498 Fragments arranged by size: 1506 1498 1255 728 445 185 153 17 13 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2466 2733 4046 Size: 274 80 33 51 1443 489 267 1313 Cuts at: 4046 5758 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1313 489 274 267 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2029 2061 3061 3093 5760 5792 2029 Size: 32 1000 32 2667 32 2037 Fragments arranged by size: 2667 2037 1000 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5800 XbaI T'CTAG_A Cuts at: 3136 3136 Size: 5800 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2479 2479 Size: 5800 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5800 XmaI C'CCGG_G Cuts at: 1870 3025 3125 1870 Size: 1155 100 4545 Fragments arranged by size: 4545 1155 100 XmnI GAAnn'nnTTC Cuts at: 2183 2830 3929 2183 Size: 647 1099 4054 Fragments arranged by size: 4054 1099 647 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3608 276 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI