Restriction map for pFN21AB7417
 (Circular) (Six-base) MAPSORT of: pFN21AB7417.seq  Check: 9047  from: 1  to: 5800



pFN21AB7417  5800 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2477    2477 
   Size:       5800
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3610     278 
   Size:         53      83     186     936    2074    2468

  Fragments arranged by size: 

                2468    2074     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3143    1937 
   Size:       1206    4594
 
Acc65I G'GTAC_C

Cuts at:   3121    3121 
   Size:       5800
 
AclI AA'CG_TT

Cuts at:   1563    3927    4300    1563 
   Size:       2364     373    3063

  Fragments arranged by size: 

                3063    2364     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2478    3863    5247    5515    1632 
   Size:        846    1385    1384     268    1917

  Fragments arranged by size: 

                1917    1385    1384     846     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5603
 
AflIII A'CryG_T

Cuts at:   1203    4699    5676    1203 
   Size:       3496     977    1327

  Fragments arranged by size: 

                3496    1327     977
 
AgeI A'CCGG_T

Cuts at:   4631    4631 
   Size:       5800
 
AhdI GACnn_n'nnGTC

Cuts at:   4529    4529 
   Size:       5800
 
AleI CACnn'nnGTG

Cuts at:   1166    2215    2309    2592    3077    1166 
   Size:       1049      94     283     485    3889

  Fragments arranged by size: 

                3889    1049     485     283      94
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5800
 
AlwNI CAG_nnn'CTG

Cuts at:   3543    5115    3543 
   Size:       1572    4228
 
ApaLI G'TGCA_C

Cuts at:   3857    5013    3857 
   Size:       1156    4644
 
ApoI r'AATT_y

Cuts at:   1493    3109    3335    3487    5596    1493 
   Size:       1616     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1616     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4354     160 
   Size:        872    3322    1606

  Fragments arranged by size: 

                3322    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5800
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3025    3125    1870 
   Size:         73    1082     100    4545

  Fragments arranged by size: 

                4545    1082     100      73
 
BamHI G'GATC_C

Cuts at:   3130    3497    3130 
   Size:        367    5433
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2522    3121    4576     618 
   Size:        325     217     627     735     599    1455    1842

  Fragments arranged by size: 

                1842    1455     735     627     599     325     217
 
BanII G_rGCy'C

Cuts at:   1391    3119    1391 
   Size:       1728    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2677     928 
   Size:        971     778    4051

  Fragments arranged by size: 

                4051     971     778
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3991    4025    1903 
   Size:         34    2054      34    3678

  Fragments arranged by size: 

                3678    2054      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2025    3692    4902    2025 
   Size:       1667    1210    2923

  Fragments arranged by size: 

                2923    1667    1210
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5800
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4411     136 
   Size:        107     122      71     631     605    2739    1525

  Fragments arranged by size: 

                2739    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5795    5795 
   Size:       5800
 
BlpI GC'TnA_GC

Cuts at:   3217    3217 
   Size:       5800
 
Bme1580I G_kGCm'C

Cuts at:   1790    2427    3861    5017    1790 
   Size:        637    1434    1156    2573

  Fragments arranged by size: 

                2573    1434    1156     637
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5800
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2586    3531    4489 
   Size:        416      53      83     399      24    1169     945     958

Cuts at:   4489     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1169     958     945     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5800
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2978    2978 
   Size:       5800
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2423    2635    3002    4460    1239 
   Size:       1184     212     367    1458    2579

  Fragments arranged by size: 

                2579    1458    1184     367     212
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2346    3925    4790    5088    2346 
   Size:       1579     865     298    3058

  Fragments arranged by size: 

                3058    1579     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2742    4463     882 
   Size:        617    1243    1721    2219

  Fragments arranged by size: 

                2219    1721    1243     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2370    3061    3591     493 
   Size:        711    1166     691     530    2702

  Fragments arranged by size: 

                2702    1166     711     691     530
 
BsaBI GATnn'nnATC

Cuts at:   5794    5794 
   Size:       5800
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3607    3989    4624     275 
   Size:         53      83     186     936    2074     382     635    1451

  Fragments arranged by size: 

                2074    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4233    4631    4905    5052    5463    4233 
   Size:        398     274     147     411    4570

  Fragments arranged by size: 

                4570     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2660    2690    3038    3068    1178 
   Size:         30     165      30    1257      30     348      30    3910

  Fragments arranged by size: 

                3910    1257     348     165      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5800
 
BseYI C'CCAG_C

Cuts at:   3065    5003    5499    3065 
   Size:       1938     496    3366

  Fragments arranged by size: 

                3366    1938     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    3007    3101    1163 
   Size:        573    1271      94    3862

  Fragments arranged by size: 

                3862    1271     573      94
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3250    4011    5039    1540 
   Size:        275     186    1249     761    1028    2301

  Fragments arranged by size: 

                2301    1249    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3861    3946    5017    3861 
   Size:         85    1071    4644

  Fragments arranged by size: 

                4644    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3311    3404    3311 
   Size:         93    5707
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2427    3119    3861    3946    5017    1391 
   Size:        399     637     692     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     692     637     399      85
 
BspEI T'CCGG_A

Cuts at:   5463    5463 
   Size:       5800
 
BspHI T'CATG_A

Cuts at:   3689    5419    3689 
   Size:       1730    4070
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2477    3155     844 
   Size:       1633     678    3489

  Fragments arranged by size: 

                3489    1633     678
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3687    1394 
   Size:        429    1864    3507

  Fragments arranged by size: 

                3507    1864     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3007    4295    4469      65 
   Size:       1183    1759    1288     174    1396

  Fragments arranged by size: 

                1759    1396    1288    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4444    4631    1700 
   Size:        110    2634     187    2869

  Fragments arranged by size: 

                2869    2634     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5800
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5800
 
BssSI C'ACGA_G

Cuts at:   3860    4872    3860 
   Size:       1012    4788
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    3018     794 
   Size:       2224    3576
 
BstBI TT'CG_AA

Cuts at:   4604    4604 
   Size:       5800
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5800
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3065    3065 
   Size:       5800
 
BstYI r'GATC_y

Cuts at:   1972    2286    3130    3497    3882    3899    5351    5437    5449 
   Size:        314     844     367     385      17    1452      86      12

Cuts at:   5449    5795    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     844     385     367     346     314      86
                  17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2453    2453 
   Size:       5800
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4309

  Fragments arranged by size: 

                4309     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5061

  Fragments arranged by size: 

                5061     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2995    3316    4110    4130    2995 
   Size:        321     794      20    4665

  Fragments arranged by size: 

                4665     794     321      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5800
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5765
 
DraI TTT'AAA

Cuts at:   3104    3104 
   Size:       5800
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4807     809 
   Size:        507    3491    1802

  Fragments arranged by size: 

                3491    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3247    4136    5765 
   Size:         54    1425      50     275      18    1417     889    1629

Cuts at:   5765       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1417     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3247    1537 
   Size:        275    1435    4090

  Fragments arranged by size: 

                4090    1435     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3730    1353 
   Size:       2377    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4387    4761    4907     387 
   Size:       1064     138    2798     374     146    1280

  Fragments arranged by size: 

                2798    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2423    2478    2635    3002    3863    4460    5247 
   Size:        393     791      55     157     367     861     597     787

Cuts at:   5247    5515    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524     861     791     787     597     393     367     268
                 157      55
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2402    2402 
   Size:       5800
 
EcoRI G'AATT_C

Cuts at:   3109    3487    3109 
   Size:        378    5422
 
FspI TGC'GCA

Cuts at:   4306    4306 
   Size:       5800
 
HaeII r_GCGC'y

Cuts at:   1678    3088    4947    1678 
   Size:       1410    1859    2531

  Fragments arranged by size: 

                2531    1859    1410
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2989    3021    3038    3070    4441 
   Size:         32     135      32    1417      32      17      32    1371

Cuts at:   4441    4473    4515    4547    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1417    1371     135      42      32      32      32
                  32      32      32      17
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2082    3144    3390     669 
   Size:        988     281     144    1062     246    3079

  Fragments arranged by size: 

                3079    1062     988     281     246     144
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5800
 
HpaI GTT'AAC

Cuts at:   3390    3390 
   Size:       5800
 
KpnI G_GTAC'C

Cuts at:   3125    3125 
   Size:       5800
 
MfeI C'AATT_G

Cuts at:   3399    3399 
   Size:       5800
 
MluI A'CGCG_T

Cuts at:   5676    5676 
   Size:       5800
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2898    4914    5098    1305 
   Size:       1593    2016     184    2007

  Fragments arranged by size: 

                2016    2007    1593     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3978

  Fragments arranged by size: 

                3978    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2215    2309    2592    3077    3758    4117    4276 
   Size:        648    1049      94     283     485     681     359     159

Cuts at:   4276     518 
   Size:       2042

  Fragments arranged by size: 

                2042    1049     681     648     485     359     283     159
                  94
 
MspA1I CmG'CkG

Cuts at:   1606    2389    3216    3893    5041    5286    1606 
   Size:        783     827     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     827     783     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4309

  Fragments arranged by size: 

                4309     939     552
 
NdeI CA'TA_TG

Cuts at:    387    2784     387 
   Size:       2397    3403
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5800
 
NotI GC'GGCC_GC

Cuts at:   3247    3247 
   Size:       5800
 
NspI r_CATG'y

Cuts at:   1132    1229    3158    4703    1132 
   Size:         97    1929    1545    2229

  Fragments arranged by size: 

                2229    1929    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5800
 
PciI A'CATG_T

Cuts at:   4699    4699 
   Size:       5800
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2737    1108 
   Size:       1629    4171
 
PmeI GTTT'AAAC

Cuts at:   3104    3104 
   Size:       5800
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3869    3901    5382    5414    3869 
   Size:         32    1481      32    4255

  Fragments arranged by size: 

                4255    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5800
 
PsiI TTA'TAA

Cuts at:   3370    3370 
   Size:       5800
 
PstI C_TGCA'G

Cuts at:    830    2378    3152     830 
   Size:       1548     774    3478

  Fragments arranged by size: 

                3478    1548     774
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5800
 
PvuII CAG'CTG

Cuts at:   2389    2389 
   Size:       5800
 
SalI G'TCGA_C

Cuts at:   1936    3142    1936 
   Size:       1206    4594
 
SbfI CC_TGCA'GG

Cuts at:   3152    3152 
   Size:       5800
 
ScaI AGT'ACT

Cuts at:   4048    5760    4048 
   Size:       1712    4088
 
SexAI A'CCwGG_T

Cuts at:   2529    2529 
   Size:       5800
 
SfcI C'TryA_G

Cuts at:    826    1045    2374    3148    4283    4964    5155     826 
   Size:        219    1329     774    1135     681     191    1471

  Fragments arranged by size: 

                1471    1329    1135     774     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5800
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5800
 
SmaI CCC'GGG

Cuts at:   1872    3027    3127    1872 
   Size:       1155     100    4545

  Fragments arranged by size: 

                4545    1155     100
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2361    3904    4805    5067     820 
   Size:        197     926     418    1543     901     262    1553

  Fragments arranged by size: 

                1553    1543     926     901     418     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2370    3591     493 
   Size:       1877    1221    2702

  Fragments arranged by size: 

                2702    1877    1221
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5800
 
SphI G_CATG'C

Cuts at:   1132    3158    1132 
   Size:       2026    3774
 
SspI AAT'ATT

Cuts at:      5      52    2195    2830    3724       5 
   Size:         47    2143     635     894    2081

  Fragments arranged by size: 

                2143    2081     894     635      47
 
StuI AGG'CCT

Cuts at:   1517    2776    2798    1517 
   Size:       1259      22    4519

  Fragments arranged by size: 

                4519    1259      22
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2173    2629    3239     513 
   Size:        552     939     169     456     610    3074

  Fragments arranged by size: 

                3074     939     610     552     456     169
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2308    2321    3827    3844    3997    4182    4627 
   Size:       1255     728      13    1506      17     153     185     445

Cuts at:   4627     325 
   Size:       1498

  Fragments arranged by size: 

                1506    1498    1255     728     445     185     153      17
                  13
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2466    2733    4046 
   Size:        274      80      33      51    1443     489     267    1313

Cuts at:   4046    5758      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443    1313     489     274     267     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2029    2061    3061    3093    5760    5792    2029 
   Size:         32    1000      32    2667      32    2037

  Fragments arranged by size: 

                2667    2037    1000      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5800
 
XbaI T'CTAG_A

Cuts at:   3136    3136 
   Size:       5800
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2479    2479 
   Size:       5800
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5800
 
XmaI C'CCGG_G

Cuts at:   1870    3025    3125    1870 
   Size:       1155     100    4545

  Fragments arranged by size: 

                4545    1155     100
 
XmnI GAAnn'nnTTC

Cuts at:   2183    2830    3929    2183 
   Size:        647    1099    4054

  Fragments arranged by size: 

                4054    1099     647
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3608     276 
   Size:         53      83     186     936    2074    2468

  Fragments arranged by size: 

                2468    2074     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoNI    EcoRI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI
    PvuII     SalI     SbfI     ScaI    SexAI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI   Bpu10I    BsiWI    BsmBI  BstZ17I   DraIII  EcoICRI EcoO109I
    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PfoI     PmlI    PpuMI   PspOMI    PspXI
     PsrI    RsrII     SacI    SacII    SanDI     SapI     SfiI     SfoI
     SrfI     SwaI
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