Restriction map for pFN21AB7359
 (Circular) (Six-base) MAPSORT of: pFN21AB7359.seq  Check: 2703  from: 1  to: 5722



pFN21AB7359  5722 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:26  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3532     278 
   Size:         53      83     186     936    1996    2468

  Fragments arranged by size: 

                2468    1996     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3065    1937 
   Size:       1128    4594
 
Acc65I G'GTAC_C

Cuts at:   3043    3043 
   Size:       5722
 
AclI AA'CG_TT

Cuts at:   1563    3849    4222    1563 
   Size:       2286     373    3063

  Fragments arranged by size: 

                3063    2286     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3785    5169    5437    1632 
   Size:       2153    1384     268    1917

  Fragments arranged by size: 

                2153    1917    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5525
 
AflIII A'CryG_T

Cuts at:   1203    2313    4621    5598    1203 
   Size:       1110    2308     977    1327

  Fragments arranged by size: 

                2308    1327    1110     977
 
AgeI A'CCGG_T

Cuts at:   4553    4553 
   Size:       5722
 
AhdI GACnn_n'nnGTC

Cuts at:   4451    4451 
   Size:       5722
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5722
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2216    2424    2781    1184 
   Size:       1032     208     357    4125

  Fragments arranged by size: 

                4125    1032     357     208
 
AlwNI CAG_nnn'CTG

Cuts at:   3465    5037    3465 
   Size:       1572    4150
 
ApaLI G'TGCA_C

Cuts at:   2065    3779    4935    2065 
   Size:       1714    1156    2852

  Fragments arranged by size: 

                2852    1714    1156
 
ApoI r'AATT_y

Cuts at:   1493    2417    2730    3004    3031    3257    3409    5518    1493 
   Size:        924     313     274      27     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     924     313     274     226     152      27
            
 
AseI AT'TA_AT

Cuts at:    160    1032    4276     160 
   Size:        872    3244    1606

  Fragments arranged by size: 

                3244    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5722
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3047    1870 
   Size:         73    1104    4545

  Fragments arranged by size: 

                4545    1104      73
 
BamHI G'GATC_C

Cuts at:   3052    3419    3052 
   Size:        367    5355
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3043    4498     618 
   Size:        325     217     627    1256    1455    1842

  Fragments arranged by size: 

                1842    1455    1256     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    3041    1391 
   Size:       1650    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2620    2629     928 
   Size:        971     721       9    4021

  Fragments arranged by size: 

                4021     971     721       9
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2380    2414    3913    3947    1903 
   Size:         34     443      34    1499      34    3678

  Fragments arranged by size: 

                3678    1499     443      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3614    4824    3614 
   Size:       1210    4512
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5722
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4333     136 
   Size:        107     122      71     631     605    2661    1525

  Fragments arranged by size: 

                2661    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5717    5717 
   Size:       5722
 
BlpI GC'TnA_GC

Cuts at:   3139    3139 
   Size:       5722
 
Bme1580I G_kGCm'C

Cuts at:   1790    2069    3783    4939    1790 
   Size:        279    1714    1156    2573

  Fragments arranged by size: 

                2573    1714    1156     279
 
BmgBI CAC'GTC

Cuts at:   1318    2053    1318 
   Size:        735    4987
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3453    4411     442 
   Size:        416      53      83     399      24    2036     958    1753

  Fragments arranged by size: 

                2036    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5722
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4382    1239 
   Size:       3143    2579
 
Bpu10I CC'TnA_GC

Cuts at:   2412    2412 
   Size:       5722
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3847    4712    5010    3847 
   Size:        865     298    4559

  Fragments arranged by size: 

                4559     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4385     882 
   Size:        617    2886    2219

  Fragments arranged by size: 

                2886    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3513     493 
   Size:        711    2309    2702

  Fragments arranged by size: 

                2702    2309     711
 
BsaBI GATnn'nnATC

Cuts at:   5716    5716 
   Size:       5722
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3529    3911    4546     275 
   Size:         53      83     186     936    1996     382     635    1451

  Fragments arranged by size: 

                1996    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2919    4155    4553    4827    4974    5385    2919 
   Size:       1236     398     274     147     411    3256

  Fragments arranged by size: 

                3256    1236     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2456    2486    1178 
   Size:         30     165      30    1053      30    4414

  Fragments arranged by size: 

                4414    1053     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5722
 
BseYI C'CCAG_C

Cuts at:   2320    2380    2553    4925    5421    2320 
   Size:         60     173    2372     496    2621

  Fragments arranged by size: 

                2621    2372     496     173      60
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2967    1163 
   Size:        573    1231    3918

  Fragments arranged by size: 

                3918    1231     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3172    3933    4961    1540 
   Size:        275     186    1171     761    1028    2301

  Fragments arranged by size: 

                2301    1171    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2069    3783    3868    4939    2069 
   Size:       1714      85    1071    2852

  Fragments arranged by size: 

                2852    1714    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2176    2672    3233    3326    2176 
   Size:        496     561      93    4572

  Fragments arranged by size: 

                4572     561     496      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2025    2025 
   Size:       5722
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2069    3041    3783    3868    4939    1391 
   Size:        399     279     972     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     972     742     399     279      85
 
BspEI T'CCGG_A

Cuts at:   2919    5385    2919 
   Size:       2466    3256
 
BspHI T'CATG_A

Cuts at:   2446    3611    5341    2446 
   Size:       1165    1730    2827

  Fragments arranged by size: 

                2827    1730    1165
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3077     844 
   Size:       2233    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2243    3609    1394 
   Size:        429     420    1366    3507

  Fragments arranged by size: 

                3507    1366     429     420
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4217    4391      65 
   Size:       1183    2969     174    1396

  Fragments arranged by size: 

                2969    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4366    4553    1700 
   Size:        110    2556     187    2869

  Fragments arranged by size: 

                2869    2556     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5722
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5722
 
BssSI C'ACGA_G

Cuts at:   3782    4794    3782 
   Size:       1012    4710
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2160     794 
   Size:       1366    4356
 
BstBI TT'CG_AA

Cuts at:   4526    4526 
   Size:       5722
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5722
 
BstYI r'GATC_y

Cuts at:   1972    2498    3052    3419    3804    3821    5273    5359    5371 
   Size:        526     554     367     385      17    1452      86      12

Cuts at:   5371    5717    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     554     526     385     367     346      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    5170
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4983

  Fragments arranged by size: 

                4983     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2281    2286    2734    2955    2988    3238    4032    4052    2281 
   Size:          5     448     221      33     250     794      20    3951

  Fragments arranged by size: 

                3951     794     448     250     221      33      20       5
            
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5722
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2275    2310     529 
   Size:         35    1711      35    3941

  Fragments arranged by size: 

                3941    1711      35      35
 
DraI TTT'AAA

Cuts at:   2595    3026    2595 
   Size:        431    5291
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4729     809 
   Size:        507    3413    1802

  Fragments arranged by size: 

                3413    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2980    3169    4058 
   Size:         54    1425      50     275      18    1150     189     889

Cuts at:   4058    5687       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425    1150     889     275     189      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3169    1537 
   Size:        275    1357    4090

  Fragments arranged by size: 

                4090    1357     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2559    3652    1353 
   Size:       1206    1093    3423

  Fragments arranged by size: 

                3423    1206    1093
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4309    4683    4829     387 
   Size:       1064     138    2720     374     146    1280

  Fragments arranged by size: 

                2720    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    3785    4382    5169    5437    1239 
   Size:        393    2153     597     787     268    1524

  Fragments arranged by size: 

                2153    1524     787     597     393     268
 
EcoRI G'AATT_C

Cuts at:   2417    3004    3031    3409    2417 
   Size:        587      27     378    4730

  Fragments arranged by size: 

                4730     587     378      27
 
EcoRV GAT'ATC

Cuts at:   2044    2044 
   Size:       5722
 
FspI TGC'GCA

Cuts at:   4228    4228 
   Size:       5722
 
HaeII r_GCGC'y

Cuts at:   1678    4869    1678 
   Size:       3191    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2324    2356    2483    2515    4363 
   Size:         32     135      32     752      32     127      32    1848

Cuts at:   4363    4395    4437    4469    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1848     752     135     127      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3066    3312     669 
   Size:        988     281    1128     246    3079

  Fragments arranged by size: 

                3079    1128     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5722
 
HpaI GTT'AAC

Cuts at:   3312    3312 
   Size:       5722
 
KpnI G_GTAC'C

Cuts at:   3047    3047 
   Size:       5722
 
MfeI C'AATT_G

Cuts at:   3321    3321 
   Size:       5722
 
MluI A'CGCG_T

Cuts at:   5598    5598 
   Size:       5722
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4836    5020    1305 
   Size:       3531     184    2007

  Fragments arranged by size: 

                3531    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2982      10 
   Size:         54    1425     343    1150    2750

  Fragments arranged by size: 

                2750    1425    1150     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2964    3680    4039    4198     518 
   Size:        648    1798     716     359     159    2042

  Fragments arranged by size: 

                2042    1798     716     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2206    2557    3138    3815    4963    5208    1606 
   Size:        600     351     581     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     600     581     351     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5170
 
NdeI CA'TA_TG

Cuts at:    387    2488     387 
   Size:       2101    3621
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5722
 
NotI GC'GGCC_GC

Cuts at:   3169    3169 
   Size:       5722
 
NspI r_CATG'y

Cuts at:   1132    1229    2163    2317    3080    4625    1132 
   Size:         97     934     154     763    1545    2229

  Fragments arranged by size: 

                2229    1545     934     763     154      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5722
 
PciI A'CATG_T

Cuts at:   2313    4621    2313 
   Size:       2308    3414
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5722
 
PfoI T'CCnGG_A

Cuts at:   2474    2474 
   Size:       5722
 
PmeI GTTT'AAAC

Cuts at:   3026    3026 
   Size:       5722
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3791    3823    5304    5336    3791 
   Size:         32    1481      32    4177

  Fragments arranged by size: 

                4177    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5722
 
PsiI TTA'TAA

Cuts at:   3292    3292 
   Size:       5722
 
PstI C_TGCA'G

Cuts at:    830    2032    2195    2519    3074     830 
   Size:       1202     163     324     555    3478

  Fragments arranged by size: 

                3478    1202     555     324     163
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5722
 
PvuII CAG'CTG

Cuts at:   2557    2557 
   Size:       5722
 
SalI G'TCGA_C

Cuts at:   1936    3064    1936 
   Size:       1128    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2559    2559 
   Size:       5722
 
SbfI CC_TGCA'GG

Cuts at:   3074    3074 
   Size:       5722
 
ScaI AGT'ACT

Cuts at:   3970    5682    3970 
   Size:       1712    4010
 
SfcI C'TryA_G

Cuts at:    826    1045    2028    2191    2515    3070    4205    4886    5077 
   Size:        219     983     163     324     555    1135     681     191

Cuts at:   5077     826 
   Size:       1471

  Fragments arranged by size: 

                1471    1135     983     681     555     324     219     191
                 163
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5722
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5722
 
SmaI CCC'GGG

Cuts at:   1872    3049    1872 
   Size:       1177    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3826    4727    4989     820 
   Size:        197     926    1883     901     262    1553

  Fragments arranged by size: 

                1883    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3513     493 
   Size:       3020    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5722
 
SphI G_CATG'C

Cuts at:   1132    3080    1132 
   Size:       1948    3774
 
SspI AAT'ATT

Cuts at:      5      52    2352    3646       5 
   Size:         47    2300    1294    2081

  Fragments arranged by size: 

                2300    2081    1294      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5722
 
StyI C'CwwG_G

Cuts at:    513    1065    3161     513 
   Size:        552    2096    3074

  Fragments arranged by size: 

                3074    2096     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2567    3029    3749    3766    3919    4104    4549 
   Size:       1255     987     462     720      17     153     185     445

Cuts at:   4549     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1255     987     720     462     445     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3968    5680      96 
   Size:        274      80      33      51    1443    1991    1712     138

  Fragments arranged by size: 

                1991    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5682    5714    5682 
   Size:         32    5690
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2336    1316 
   Size:       1020    4702
 
XbaI T'CTAG_A

Cuts at:   3058    3058 
   Size:       5722
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2352    2352 
   Size:       5722
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5722
 
XmaI C'CCGG_G

Cuts at:   1870    3047    1870 
   Size:       1177    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2217    3851    2217 
   Size:       1634    4088
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3530     276 
   Size:         53      83     186     936    1996    2468

  Fragments arranged by size: 

                2468    1996     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoRI    EcoRV     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI    PvuII
     SalI     SapI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI    BsiWI    BstXI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoNI EcoO109I     FalI     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI     PmlI    PpuMI   PspOMI
    PspXI     PsrI    RsrII     SacI    SacII    SanDI    SexAI     SfiI
     SfoI     SrfI     SwaI
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