Restriction map for
pFN21AB7298
(Linear) (Six-base) MAPSORT of: KIBB7298.seq Check: 1507 from: 1 to: 3051
KIBB7298.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AccI GT'mk_AC
Cuts at: 0 128 3051
Size: 128 2923
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1027 2882 3051
Size: 1027 1855 169
Fragments arranged by size:
1855 1027 169
AflIII A'CryG_T
Cuts at: 0 587 1331 3041 3051
Size: 587 744 1710 10
Fragments arranged by size:
1710 744 587 10
AleI CACnn'nnGTG
Cuts at: 0 856 1315 3051
Size: 856 459 1736
Fragments arranged by size:
1736 856 459
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 103 3051
Size: 103 2948
AlwNI CAG_nnn'CTG
Cuts at: 0 246 1050 1688 1968 2905 2956 3051
Size: 246 804 638 280 937 51 95
Fragments arranged by size:
937 804 638 280 246 95 51
ApaI G_GGCC'C
Cuts at: 0 1701 3051
Size: 1701 1350
ApaLI G'TGCA_C
Cuts at: 0 997 1501 3051
Size: 997 504 1550
Fragments arranged by size:
1550 997 504
ApoI r'AATT_y
Cuts at: 0 490 3051
Size: 490 2561
AvaI C'yCGr_G
Cuts at: 0 613 750 2042 2261 3051
Size: 613 137 1292 219 790
Fragments arranged by size:
1292 790 613 219 137
BanI G'GyrC_C
Cuts at: 0 323 1151 1227 1387 2571 3051
Size: 323 828 76 160 1184 480
Fragments arranged by size:
1184 828 480 323 160 76
BanII G_rGCy'C
Cuts at: 0 155 1701 2074 2269 3051
Size: 155 1546 373 195 782
Fragments arranged by size:
1546 782 373 195 155
BbeI G_GCGC'C
Cuts at: 0 1155 1391 2575 3051
Size: 1155 236 1184 476
Fragments arranged by size:
1184 1155 476 236
BbsI GAAGACnn'nnnn_
Cuts at: 0 193 513 1015 3051
Size: 193 320 502 2036
Fragments arranged by size:
2036 502 320 193
BbvCI CC'TCA_GC
Cuts at: 0 1988 2089 2245 2423 2513 2618 2794 3051
Size: 1988 101 156 178 90 105 176 257
Fragments arranged by size:
1988 257 178 176 156 105 101 90
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 922 956 1624 1658 3051
Size: 922 34 668 34 1393
Fragments arranged by size:
1393 922 668 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2841 3051
Size: 2841 210
BglI GCCn_nnn'nGGC
Cuts at: 0 548 557 566 1771 2029 2223 2285 2614
Size: 548 9 9 1205 258 194 62 329
Cuts at: 2614 3051
Size: 437
Fragments arranged by size:
1205 548 437 329 258 194 62 9
9
BglII A'GATC_T
Cuts at: 0 1358 2657 3051
Size: 1358 1299 394
Fragments arranged by size:
1358 1299 394
BlpI GC'TnA_GC
Cuts at: 0 312 1969 2689 3051
Size: 312 1657 720 362
Fragments arranged by size:
1657 720 362 312
Bme1580I G_kGCm'C
Cuts at: 0 109 614 1001 1232 1505 1701 3051
Size: 109 505 387 231 273 196 1350
Fragments arranged by size:
1350 505 387 273 231 196 109
BmrI ACTGGGnnnn_n'
Cuts at: 0 858 2452 3051
Size: 858 1594 599
Fragments arranged by size:
1594 858 599
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1077 3051
Size: 1077 1974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 1072 1243 2827 2960 3051
Size: 280 792 171 1584 133 91
Fragments arranged by size:
1584 792 280 171 133 91
Bpu10I CC'TnA_GC
Cuts at: 0 1871 1988 2089 2245 2423 2513 2618 2794
Size: 1871 117 101 156 178 90 105 176
Cuts at: 2794 3051
Size: 257
Fragments arranged by size:
1871 257 178 176 156 117 105 101
90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1468 1507 1822 2940 3033 3051
Size: 1468 39 315 1118 93 18
Fragments arranged by size:
1468 1118 315 93 39 18
BsaI GGTCTCn'nnnn_
Cuts at: 0 214 647 2916 3051
Size: 214 433 2269 135
Fragments arranged by size:
2269 433 214 135
BsaAI yAC'GTr
Cuts at: 0 1556 3051
Size: 1556 1495
BsaBI GATnn'nnATC
Cuts at: 0 343 3051
Size: 343 2708
BsaHI Gr'CG_yC
Cuts at: 0 1152 1388 1580 2572 3051
Size: 1152 236 192 992 479
Fragments arranged by size:
1152 992 479 236 192
BsaWI w'CCGG_w
Cuts at: 0 2460 3051
Size: 2460 591
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 445 475 655 685 1876 1906 2213 2243
Size: 445 30 180 30 1191 30 307 30
Cuts at: 2243 2362 2392 2825 2855 3051
Size: 119 30 433 30 196
Fragments arranged by size:
1191 445 433 307 196 180 119 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 643 646 878 2136 2139 2305 2310 2635
Size: 643 3 232 1258 3 166 5 325
Cuts at: 2635 3051
Size: 416
Fragments arranged by size:
1258 643 416 325 232 166 5 3
3
BseYI C'CCAG_C
Cuts at: 0 2120 2250 2593 2733 2751 2836 2874 3051
Size: 2120 130 343 140 18 85 38 177
Fragments arranged by size:
2120 343 177 140 130 85 38 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 1089 1356 1693 3051
Size: 232 857 267 337 1358
Fragments arranged by size:
1358 857 337 267 232
BsiEI CG_ry'CG
Cuts at: 0 168 1519 3051
Size: 168 1351 1532
Fragments arranged by size:
1532 1351 168
BsiHKAI G_wGCw'C
Cuts at: 0 155 278 1001 1085 1505 1811 2404 3051
Size: 155 123 723 84 420 306 593 647
Fragments arranged by size:
723 647 593 420 306 155 123 84
BsmBI CGTCTCn'nnnn_
Cuts at: 0 675 2671 3051
Size: 675 1996 380
Fragments arranged by size:
1996 675 380
Bsp1286I G_dGCh'C
Cuts at: 0 109 155 278 614 1001 1085 1232 1505
Size: 109 46 123 336 387 84 147 273
Cuts at: 1505 1701 1811 2074 2269 2404 3051
Size: 196 110 263 195 135 647
Fragments arranged by size:
647 387 336 273 263 196 195 147
135 123 110 109 84 46
BspEI T'CCGG_A
Cuts at: 0 2460 3051
Size: 2460 591
BspHI T'CATG_A
Cuts at: 0 8 3051
Size: 8 3043
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 199 968 2069 3051
Size: 199 769 1101 982
Fragments arranged by size:
1101 982 769 199
BsrBI CCG'CTC
Cuts at: 0 1056 1454 1521 2129 2564 3051
Size: 1056 398 67 608 435 487
Fragments arranged by size:
1056 608 487 435 398 67
BsrDI GCAATG_nn'
Cuts at: 0 1625 3051
Size: 1625 1426
BsrFI r'CCGG_y
Cuts at: 0 3 538 2642 3051
Size: 3 535 2104 409
Fragments arranged by size:
2104 535 409 3
BstAPI GCAn_nnn'nTGC
Cuts at: 0 246 1509 1533 1688 3051
Size: 246 1263 24 155 1363
Fragments arranged by size:
1363 1263 246 155 24
BstEII G'GTnAC_C
Cuts at: 0 723 3051
Size: 723 2328
BstYI r'GATC_y
Cuts at: 0 1358 1649 2657 3051
Size: 1358 291 1008 394
Fragments arranged by size:
1358 1008 394 291
Bsu36I CC'TnA_GG
Cuts at: 0 1996 3051
Size: 1996 1055
BtgI C'CryG_G
Cuts at: 0 438 632 806 1513 1755 2891 3051
Size: 438 194 174 707 242 1136 160
Fragments arranged by size:
1136 707 438 242 194 174 160
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 36 786 1263 3051
Size: 36 750 477 1788
Fragments arranged by size:
1788 750 477 36
BtsI GCAGTG_nn'
Cuts at: 0 1077 2868 3051
Size: 1077 1791 183
Fragments arranged by size:
1791 1077 183
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 45 80 3051
Size: 45 35 2971
Fragments arranged by size:
2971 45 35
DraIII CAC_nnn'GTG
Cuts at: 0 1404 3051
Size: 1404 1647
EaeI y'GGCC_r
Cuts at: 0 92 540 549 870 914 1516 1829 2361
Size: 92 448 9 321 44 602 313 532
Cuts at: 2361 2606 3051
Size: 245 445
Fragments arranged by size:
602 532 448 445 321 313 245 92
44 9
EagI C'GGCC_G
Cuts at: 0 1516 3051
Size: 1516 1535
EarI CTCTTCn'nnn_
Cuts at: 0 823 3051
Size: 823 2228
EcoICRI GAG'CTC
Cuts at: 0 153 3051
Size: 153 2898
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 1027 1072 1243 2827 2882 2960 3051
Size: 280 747 45 171 1584 55 78 91
Fragments arranged by size:
1584 747 280 171 91 78 55 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1840 2065 2722 3051
Size: 1840 225 657 329
Fragments arranged by size:
1840 657 329 225
EcoO109I rG'GnC_Cy
Cuts at: 0 1479 1698 1848 1992 2046 2216 2517 2558
Size: 1479 219 150 144 54 170 301 41
Cuts at: 2558 2737 2968 3051
Size: 179 231 83
Fragments arranged by size:
1479 301 231 219 179 170 150 144
83 54 41
EcoRV GAT'ATC
Cuts at: 0 396 831 1866 3051
Size: 396 435 1035 1185
Fragments arranged by size:
1185 1035 435 396
HaeII r_GCGC'y
Cuts at: 0 1155 1274 1391 2575 3051
Size: 1155 119 117 1184 476
Fragments arranged by size:
1184 1155 476 119 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1850 1882 1989 2021 2213 2245 2649 2681
Size: 1850 32 107 32 192 32 404 32
Cuts at: 2681 3051
Size: 370
Fragments arranged by size:
1850 404 370 192 107 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1566 3051
Size: 1566 1485
KasI G'GCGC_C
Cuts at: 0 1151 1387 2571 3051
Size: 1151 236 1184 480
Fragments arranged by size:
1184 1151 480 236
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 3051
Size: 161 2890
MscI TGG'CCA
Cuts at: 0 94 916 1831 2363 2608 3051
Size: 94 822 915 532 245 443
Fragments arranged by size:
915 822 532 443 245 94
MslI CAynn'nnrTG
Cuts at: 0 805 856 982 1315 1498 1630 1781 3051
Size: 805 51 126 333 183 132 151 1270
Fragments arranged by size:
1270 805 333 183 151 132 126 51
MspA1I CmG'CkG
Cuts at: 0 197 440 1179 1515 1571 1659 1968 2512
Size: 197 243 739 336 56 88 309 544
Cuts at: 2512 2793 3051
Size: 281 258
Fragments arranged by size:
739 544 336 309 281 258 243 197
88 56
NaeI GCC'GGC
Cuts at: 0 2644 3051
Size: 2644 407
NarI GG'CG_CC
Cuts at: 0 1152 1388 2572 3051
Size: 1152 236 1184 479
Fragments arranged by size:
1184 1152 479 236
NcoI C'CATG_G
Cuts at: 0 2891 3051
Size: 2891 160
NgoMIV G'CCGG_C
Cuts at: 0 2642 3051
Size: 2642 409
NotI GC'GGCC_GC
Cuts at: 0 1516 3051
Size: 1516 1535
NruI TCG'CGA
Cuts at: 0 780 3051
Size: 780 2271
NspI r_CATG'y
Cuts at: 0 1443 1536 3051
Size: 1443 93 1515
Fragments arranged by size:
1515 1443 93
PflMI CCAn_nnn'nTGG
Cuts at: 0 421 912 1125 3051
Size: 421 491 213 1926
Fragments arranged by size:
1926 491 421 213
PfoI T'CCnGG_A
Cuts at: 0 41 329 2843 2912 3051
Size: 41 288 2514 69 139
Fragments arranged by size:
2514 288 139 69 41
PmlI CAC'GTG
Cuts at: 0 1556 3051
Size: 1556 1495
PpuMI rG'GwC_Cy
Cuts at: 0 1479 1848 1992 2046 2737 2968 3051
Size: 1479 369 144 54 691 231 83
Fragments arranged by size:
1479 691 369 231 144 83 54
PshAI GACnn'nnGTC
Cuts at: 0 2468 3051
Size: 2468 583
PspOMI G'GGCC_C
Cuts at: 0 1697 3051
Size: 1697 1354
PstI C_TGCA'G
Cuts at: 0 196 242 1046 1658 1772 1964 2726 3051
Size: 196 46 804 612 114 192 762 325
Fragments arranged by size:
804 762 612 325 196 192 114 46
PvuII CAG'CTG
Cuts at: 0 197 1659 1968 2512 2793 3051
Size: 197 1462 309 544 281 258
Fragments arranged by size:
1462 544 309 281 258 197
SacI G_AGCT'C
Cuts at: 0 155 3051
Size: 155 2896
SacII CC_GC'GG
Cuts at: 0 441 1516 3051
Size: 441 1075 1535
Fragments arranged by size:
1535 1075 441
SanDI GG'GwC_CC
Cuts at: 0 2737 2968 3051
Size: 2737 231 83
Fragments arranged by size:
2737 231 83
SapI GCTCTTCn'nnn_
Cuts at: 0 823 3051
Size: 823 2228
SbfI CC_TGCA'GG
Cuts at: 0 1772 3051
Size: 1772 1279
SexAI A'CCwGG_T
Cuts at: 0 919 3051
Size: 919 2132
SfcI C'TryA_G
Cuts at: 0 192 238 663 1042 1654 1768 1960 2579
Size: 192 46 425 379 612 114 192 619
Cuts at: 2579 2722 3051
Size: 143 329
Fragments arranged by size:
619 612 425 379 329 192 192 143
114 46
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 548 557 2223 3051
Size: 548 9 1666 828
Fragments arranged by size:
1666 828 548 9
SfoI GGC'GCC
Cuts at: 0 1153 1389 2573 3051
Size: 1153 236 1184 478
Fragments arranged by size:
1184 1153 478 236
SmaI CCC'GGG
Cuts at: 0 2044 3051
Size: 2044 1007
SmlI C'TyrA_G
Cuts at: 0 1483 1522 1801 2919 3012 3051
Size: 1483 39 279 1118 93 39
Fragments arranged by size:
1483 1118 279 93 39 39
SphI G_CATG'C
Cuts at: 0 1536 3051
Size: 1536 1515
StuI AGG'CCT
Cuts at: 0 2544 2989 3051
Size: 2544 445 62
Fragments arranged by size:
2544 445 62
StyI C'CwwG_G
Cuts at: 0 429 465 674 2554 2891 3051
Size: 429 36 209 1880 337 160
Fragments arranged by size:
1880 429 337 209 160 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 485 1089 2765 2850 3051
Size: 485 604 1676 85 201
Fragments arranged by size:
1676 604 485 201 85
TatI w'GTAC_w
Cuts at: 0 617 1289 1442 3051
Size: 617 672 153 1609
Fragments arranged by size:
1609 672 617 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 445 477 2362 2394 2825 2857 3051
Size: 445 32 1885 32 431 32 194
Fragments arranged by size:
1885 445 431 194 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2601 3051
Size: 2601 450
XmaI C'CCGG_G
Cuts at: 0 2042 3051
Size: 2042 1009
XmnI GAAnn'nnTTC
Cuts at: 0 1360 3051
Size: 1360 1691
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstEII BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NruI NspI PflMI PfoI PmlI
PpuMI PshAI PspOMI PstI PvuII SacI SacII SanDI
SapI SbfI SexAI SfcI SfiI SfoI SmaI SmlI
SphI StuI StyI TaqII TatI TstI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI AscI AseI AsiSI AvrII BaeI BamHI BclI
BfrBI BmgBI BmtI BsiWI BsmI BsrGI BssHII BssSI
BstBI BstXI BstZ17I ClaI DraI DrdI EciI EcoRI
FalI FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI NdeI NheI NsiI PacI PasI PciI PmeI
PpiI PsiI PspXI PsrI PvuI RsrII SalI ScaI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I
XbaI XhoI ZraI