(Circular) (Six-base) MAPSORT of: pFN21AB7296.seq Check: 9056 from: 1 to: 8704 pFN21AB7296 8704 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6514 278 Size: 53 83 186 936 4978 2468 Fragments arranged by size: 4978 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6047 1937 Size: 4110 4594 Acc65I G'GTAC_C Cuts at: 5147 6025 5147 Size: 878 7826 AclI AA'CG_TT Cuts at: 1563 6831 7204 1563 Size: 5268 373 3063 Fragments arranged by size: 5268 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2123 2190 3618 3771 6767 8151 8419 1632 Size: 491 67 1428 153 2996 1384 268 1917 Fragments arranged by size: 2996 1917 1428 1384 491 268 153 67 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8507 AflIII A'CryG_T Cuts at: 1203 2221 3356 3358 7603 8580 1203 Size: 1018 1135 2 4245 977 1327 Fragments arranged by size: 4245 1327 1135 1018 977 2 AgeI A'CCGG_T Cuts at: 7535 7535 Size: 8704 AhdI GACnn_n'nnGTC Cuts at: 7433 7433 Size: 8704 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8704 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8704 AlwNI CAG_nnn'CTG Cuts at: 2020 3743 3818 3962 3977 5237 5486 6447 8019 Size: 1723 75 144 15 1260 249 961 1572 Cuts at: 8019 2020 Size: 2705 Fragments arranged by size: 2705 1723 1572 1260 961 249 144 75 15 ApaLI G'TGCA_C Cuts at: 6761 7917 6761 Size: 1156 7548 ApoI r'AATT_y Cuts at: 1493 2331 2637 3332 3795 3951 4151 4534 4639 Size: 838 306 695 463 156 200 383 105 Cuts at: 4639 4701 4797 5015 5245 6013 6239 6391 8500 Size: 62 96 218 230 768 226 152 2109 Cuts at: 8500 1493 Size: 1697 Fragments arranged by size: 2109 1697 838 768 695 463 383 306 230 226 218 200 156 152 105 96 62 AseI AT'TA_AT Cuts at: 160 1032 2156 2553 3507 4770 7258 160 Size: 872 1124 397 954 1263 2488 1606 Fragments arranged by size: 2488 1606 1263 1124 954 872 397 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8704 AvaI C'yCGr_G Cuts at: 1870 1943 2117 2901 6029 1870 Size: 73 174 784 3128 4545 Fragments arranged by size: 4545 3128 784 174 73 AvrII C'CTAG_G Cuts at: 2356 5555 2356 Size: 3199 5505 BamHI G'GATC_C Cuts at: 5205 6034 6401 5205 Size: 829 367 7508 Fragments arranged by size: 7508 829 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2074 5147 6025 7480 618 Size: 325 217 627 287 3073 878 1455 1842 Fragments arranged by size: 3073 1842 1455 878 627 325 287 217 BanII G_rGCy'C Cuts at: 1391 2691 5339 6023 1391 Size: 1300 2648 684 4072 Fragments arranged by size: 4072 2648 1300 684 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2862 3114 3205 4446 5479 928 Size: 971 963 252 91 1241 1033 4153 Fragments arranged by size: 4153 1241 1033 971 963 252 91 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6895 6929 1903 Size: 34 50 34 4874 34 3678 Fragments arranged by size: 4874 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6596 7806 6596 Size: 1210 7494 BclI T'GATC_A Cuts at: 1548 1548 Size: 8704 BfrBI ATG'CAT Cuts at: 4127 4127 Size: 8704 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2059 7315 Size: 107 122 71 631 605 337 50 5256 Cuts at: 7315 136 Size: 1525 Fragments arranged by size: 5256 1525 631 605 337 122 107 71 50 BglII A'GATC_T Cuts at: 4384 5396 8699 4384 Size: 1012 3303 4389 Fragments arranged by size: 4389 3303 1012 BlpI GC'TnA_GC Cuts at: 2190 6121 2190 Size: 3931 4773 Bme1580I G_kGCm'C Cuts at: 1790 4314 6765 7921 1790 Size: 2524 2451 1156 2573 Fragments arranged by size: 2573 2524 2451 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8704 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2315 2792 4099 Size: 416 53 83 399 24 898 477 1307 Cuts at: 4099 6435 7393 442 Size: 2336 958 1753 Fragments arranged by size: 2336 1753 1307 958 898 477 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3456 1056 Size: 2400 6304 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3763 4293 3763 Size: 530 8174 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 7364 1239 Size: 6125 2579 Bpu10I CC'TnA_GC Cuts at: 3963 5067 3963 Size: 1104 7600 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3900 4033 4640 5332 5668 6829 7694 7992 3900 Size: 133 607 692 336 1161 865 298 4612 Fragments arranged by size: 4612 1161 865 692 607 336 298 133 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7367 882 Size: 617 5868 2219 Fragments arranged by size: 5868 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3359 3846 6495 493 Size: 711 2155 487 2649 2702 Fragments arranged by size: 2702 2649 2155 711 487 BsaBI GATnn'nnATC Cuts at: 4128 5325 8698 4128 Size: 1197 3373 4134 Fragments arranged by size: 4134 3373 1197 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6511 6893 7528 275 Size: 53 83 186 936 4978 382 635 1451 Fragments arranged by size: 4978 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3642 5604 7137 7535 7809 7956 8367 3642 Size: 1962 1533 398 274 147 411 3979 Fragments arranged by size: 3979 1962 1533 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5685 5715 5764 5782 5794 Size: 30 165 30 4282 30 49 18 12 Cuts at: 5794 5812 1178 Size: 18 4070 Fragments arranged by size: 4282 4070 165 49 30 30 30 18 18 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3710 5445 5772 5775 5790 1186 Size: 2524 1735 327 3 15 4100 Fragments arranged by size: 4100 2524 1735 327 15 3 BseYI C'CCAG_C Cuts at: 5491 5542 5867 7907 8403 5491 Size: 51 325 2040 496 5792 Fragments arranged by size: 5792 2040 496 325 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4038 5223 1163 Size: 573 2302 1185 4644 Fragments arranged by size: 4644 2302 1185 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6154 6915 7943 1540 Size: 275 186 4153 761 1028 2301 Fragments arranged by size: 4153 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4101 5946 6765 6850 7921 4101 Size: 1845 819 85 1071 4884 Fragments arranged by size: 4884 1845 1071 819 85 BsmI GAATG_Cn' Cuts at: 5995 6215 6308 5995 Size: 220 93 8391 Fragments arranged by size: 8391 220 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2691 4101 4314 5339 5946 6023 6765 Size: 399 901 1410 213 1025 607 77 742 Cuts at: 6765 6850 7921 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1410 1071 1025 901 742 607 399 213 85 77 BspEI T'CCGG_A Cuts at: 8367 8367 Size: 8704 BspHI T'CATG_A Cuts at: 2926 4717 6593 8323 2926 Size: 1791 1876 1730 3307 Fragments arranged by size: 3307 1876 1791 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4338 6059 844 Size: 3494 1721 3489 Fragments arranged by size: 3494 3489 1721 BsrBI CCG'CTC Cuts at: 1394 1823 6591 1394 Size: 429 4768 3507 Fragments arranged by size: 4768 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2505 3463 4185 5359 5746 5862 7199 Size: 1183 1257 958 722 1174 387 116 1337 Cuts at: 7199 7373 65 Size: 174 1396 Fragments arranged by size: 1396 1337 1257 1183 1174 958 722 387 174 116 BsrFI r'CCGG_y Cuts at: 1700 1810 7348 7535 1700 Size: 110 5538 187 2869 Fragments arranged by size: 5538 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4399 96 Size: 4303 4401 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8704 BssSI C'ACGA_G Cuts at: 5652 6764 7776 5652 Size: 1112 1012 6580 Fragments arranged by size: 6580 1112 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2020 2504 794 Size: 1226 484 6994 Fragments arranged by size: 6994 1226 484 BstBI TT'CG_AA Cuts at: 7508 7508 Size: 8704 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8704 BstYI r'GATC_y Cuts at: 1972 3862 3899 4384 4914 5205 5396 6034 6401 Size: 1890 37 485 530 291 191 638 367 Cuts at: 6401 6786 6803 8255 8341 8353 8699 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1890 1452 638 530 485 385 367 346 291 191 86 37 17 12 Bsu36I CC'TnA_GG Cuts at: 2590 4839 2590 Size: 2249 6455 BtgI C'CryG_G Cuts at: 513 1065 5386 5734 513 Size: 552 4321 348 3483 Fragments arranged by size: 4321 3483 552 348 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7965 Fragments arranged by size: 7965 683 56 BtsI GCAGTG_nn' Cuts at: 5317 6220 7014 7034 5317 Size: 903 794 20 6987 Fragments arranged by size: 6987 903 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8704 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5964 5999 529 Size: 35 5400 35 3234 Fragments arranged by size: 5400 3234 35 35 DraI TTT'AAA Cuts at: 2430 3123 3558 6008 2430 Size: 693 435 2450 5126 Fragments arranged by size: 5126 2450 693 435 DraIII CAC_nnn'GTG Cuts at: 4503 4781 4503 Size: 278 8426 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7711 809 Size: 507 6395 1802 Fragments arranged by size: 6395 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 6151 7040 8669 Size: 54 1425 50 275 18 4321 889 1629 Cuts at: 8669 8 Size: 43 Fragments arranged by size: 4321 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6151 1537 Size: 275 4339 4090 Fragments arranged by size: 4339 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2037 2142 2571 3594 3681 3747 3765 6634 Size: 684 105 429 1023 87 66 18 2869 Cuts at: 6634 1353 Size: 3423 Fragments arranged by size: 3423 2869 1023 684 429 105 87 66 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5262 7291 7665 7811 387 Size: 1064 138 3673 2029 374 146 1280 Fragments arranged by size: 3673 2029 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2190 3618 3771 6767 7364 8151 Size: 393 491 67 1428 153 2996 597 787 Cuts at: 8151 8419 1239 Size: 268 1524 Fragments arranged by size: 2996 1524 1428 787 597 491 393 268 153 67 EcoO109I rG'GnC_Cy Cuts at: 2353 4843 2353 Size: 2490 6214 EcoRI G'AATT_C Cuts at: 3795 4639 6013 6391 3795 Size: 844 1374 378 6108 Fragments arranged by size: 6108 1374 844 378 EcoRV GAT'ATC Cuts at: 3630 4963 3630 Size: 1333 7371 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2325 3368 5418 5870 2325 Size: 1043 2050 452 5159 Fragments arranged by size: 5159 2050 1043 452 FspI TGC'GCA Cuts at: 7210 7210 Size: 8704 HaeII r_GCGC'y Cuts at: 1678 7851 1678 Size: 6173 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2261 2293 4717 4749 5594 Size: 32 135 32 689 32 2424 32 845 Cuts at: 5594 5626 5666 5698 7345 7377 7419 7451 1373 Size: 32 40 32 1647 32 42 32 2626 Fragments arranged by size: 2626 2424 1647 845 689 135 42 40 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5265 6048 6294 669 Size: 988 281 3327 783 246 3079 Fragments arranged by size: 3327 3079 988 783 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8704 HpaI GTT'AAC Cuts at: 5265 6294 5265 Size: 1029 7675 KpnI G_GTAC'C Cuts at: 5151 6029 5151 Size: 878 7826 MfeI C'AATT_G Cuts at: 2247 3610 6303 2247 Size: 1363 2693 4648 Fragments arranged by size: 4648 2693 1363 MluI A'CGCG_T Cuts at: 8580 8580 Size: 8704 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4399 5728 5964 7818 8002 1305 Size: 3094 1329 236 1854 184 2007 Fragments arranged by size: 3094 2007 1854 1329 236 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6882 Fragments arranged by size: 6882 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2538 4107 5739 5967 6662 7021 7180 Size: 648 1372 1569 1632 228 695 359 159 Cuts at: 7180 518 Size: 2042 Fragments arranged by size: 2042 1632 1569 1372 695 648 359 228 159 MspA1I CmG'CkG Cuts at: 1606 2020 3596 6120 6797 7945 8190 1606 Size: 414 1576 2524 677 1148 245 2120 Fragments arranged by size: 2524 2120 1576 1148 677 414 245 NcoI C'CATG_G Cuts at: 513 1065 5386 5734 513 Size: 552 4321 348 3483 Fragments arranged by size: 4321 3483 552 348 NdeI CA'TA_TG Cuts at: 387 2887 3852 387 Size: 2500 965 5239 Fragments arranged by size: 5239 2500 965 NheI G'CTAG_C Cuts at: 1052 3452 1052 Size: 2400 6304 NotI GC'GGCC_GC Cuts at: 6151 6151 Size: 8704 NsiI A_TGCA'T Cuts at: 4129 4129 Size: 8704 NspI r_CATG'y Cuts at: 1132 1229 3219 3542 3781 6062 7607 1132 Size: 97 1990 323 239 2281 1545 2229 Fragments arranged by size: 2281 2229 1990 1545 323 239 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8704 PciI A'CATG_T Cuts at: 7603 7603 Size: 8704 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8704 PmeI GTTT'AAAC Cuts at: 6008 6008 Size: 8704 PmlI CAC'GTG Cuts at: 3359 3359 Size: 8704 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6773 6805 8286 8318 6773 Size: 32 1481 32 7159 Fragments arranged by size: 7159 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2353 4843 2353 Size: 2490 6214 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8704 PsiI TTA'TAA Cuts at: 2364 2672 3338 3935 4871 6274 2364 Size: 308 666 597 936 1403 4794 Fragments arranged by size: 4794 1403 936 666 597 308 PstI C_TGCA'G Cuts at: 830 2422 5175 6056 830 Size: 1592 2753 881 3478 Fragments arranged by size: 3478 2753 1592 881 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8704 PvuII CAG'CTG Cuts at: 2020 3596 2020 Size: 1576 7128 SalI G'TCGA_C Cuts at: 1936 6046 1936 Size: 4110 4594 SapI GCTCTTCn'nnn_ Cuts at: 2142 2571 2142 Size: 429 8275 SbfI CC_TGCA'GG Cuts at: 6056 6056 Size: 8704 ScaI AGT'ACT Cuts at: 6952 8664 6952 Size: 1712 6992 SexAI A'CCwGG_T Cuts at: 2376 2917 4246 5143 2376 Size: 541 1329 897 5937 Fragments arranged by size: 5937 1329 897 541 SfcI C'TryA_G Cuts at: 826 1045 2418 3680 3990 5171 6052 7187 7868 Size: 219 1373 1262 310 1181 881 1135 681 Cuts at: 7868 8059 826 Size: 191 1471 Fragments arranged by size: 1471 1373 1262 1181 1135 881 681 310 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8704 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8704 SmaI CCC'GGG Cuts at: 1872 6031 1872 Size: 4159 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2117 2901 3915 4012 4619 5347 Size: 197 926 174 784 1014 97 607 728 Cuts at: 5347 5683 6808 7709 7971 820 Size: 336 1125 901 262 1553 Fragments arranged by size: 1553 1125 1014 926 901 784 728 607 336 262 197 174 97 SnaBI TAC'GTA Cuts at: 493 3846 6495 493 Size: 3353 2649 2702 Fragments arranged by size: 3353 2702 2649 SpeI A'CTAG_T Cuts at: 152 2836 152 Size: 2684 6020 SphI G_CATG'C Cuts at: 1132 3542 6062 1132 Size: 2410 2520 3774 Fragments arranged by size: 3774 2520 2410 SspI AAT'ATT Cuts at: 5 52 3289 3760 4258 4748 6628 5 Size: 47 3237 471 498 490 1880 2081 Fragments arranged by size: 3237 2081 1880 498 490 471 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8704 StyI C'CwwG_G Cuts at: 513 1065 2356 3786 5183 5386 5555 5734 6143 Size: 552 1291 1430 1397 203 169 179 409 Cuts at: 6143 513 Size: 3074 Fragments arranged by size: 3074 1430 1397 1291 552 409 203 179 169 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4359 5967 6731 6748 6901 7086 7531 Size: 1255 2779 1608 764 17 153 185 445 Cuts at: 7531 325 Size: 1498 Fragments arranged by size: 2779 1608 1498 1255 764 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3647 4399 5228 Size: 274 80 33 51 1443 1670 752 829 Cuts at: 5228 6950 8662 96 Size: 1722 1712 138 Fragments arranged by size: 1722 1712 1670 1443 829 752 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2058 2090 5685 5717 8664 8696 2058 Size: 32 3595 32 2947 32 2066 Fragments arranged by size: 3595 2947 2066 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4248 1316 Size: 2932 5772 XbaI T'CTAG_A Cuts at: 6040 6040 Size: 8704 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2253 3031 2253 Size: 778 7926 XhoI C'TCGA_G Cuts at: 1943 2117 2901 1943 Size: 174 784 7746 Fragments arranged by size: 7746 784 174 XmaI C'CCGG_G Cuts at: 1870 6029 1870 Size: 4159 4545 XmnI GAAnn'nnTTC Cuts at: 2203 2223 2331 3006 6833 2203 Size: 20 108 675 3827 4074 Fragments arranged by size: 4074 3827 675 108 20 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6512 276 Size: 53 83 186 936 4978 2468 Fragments arranged by size: 4978 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI