Restriction map for pFN21AB7296
 (Linear) (Six-base) MAPSORT of: KIBB7296.seq  Check: 9186  from: 1  to: 3999



KIBB7296.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

Acc65I G'GTAC_C

Cuts at:      0    3142    3999 
   Size:       3142     857
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     185    1613    1766    3999 
   Size:        118      67    1428     153    2233

  Fragments arranged by size: 

                2233    1428     153     118      67
 
AflIII A'CryG_T

Cuts at:      0     216    1351    1353    3999 
   Size:        216    1135       2    2646

  Fragments arranged by size: 

                2646    1135     216       2
 
AlwNI CAG_nnn'CTG

Cuts at:      0      15    1738    1813    1957    1972    3232    3481    3999 
   Size:         15    1723      75     144      15    1260     249     518

  Fragments arranged by size: 

                1723    1260     518     249     144      75      15      15
            
 
ApoI r'AATT_y

Cuts at:      0     326     632    1327    1790    1946    2146    2529    2634 
   Size:        326     306     695     463     156     200     383     105

Cuts at:   2634    2696    2792    3010    3240    3999 
   Size:         62      96     218     230     759

  Fragments arranged by size: 

                 759     695     463     383     326     306     230     218
                 200     156     105      96      62
 
AseI AT'TA_AT

Cuts at:      0     151     548    1502    2765    3999 
   Size:        151     397     954    1263    1234

  Fragments arranged by size: 

                1263    1234     954     397     151
 
AvaI C'yCGr_G

Cuts at:      0     112     896    3999 
   Size:        112     784    3103

  Fragments arranged by size: 

                3103     784     112
 
AvrII C'CTAG_G

Cuts at:      0     351    3550    3999 
   Size:        351    3199     449

  Fragments arranged by size: 

                3199     449     351
 
BamHI G'GATC_C

Cuts at:      0    3200    3999 
   Size:       3200     799
 
BanI G'GyrC_C

Cuts at:      0      69    3142    3999 
   Size:         69    3073     857

  Fragments arranged by size: 

                3073     857      69
 
BanII G_rGCy'C

Cuts at:      0     686    3334    3999 
   Size:        686    2648     665

  Fragments arranged by size: 

                2648     686     665
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     857    1109    1200    2441    3474    3999 
   Size:        857     252      91    1241    1033     525

  Fragments arranged by size: 

                1241    1033     857     525     252      91
 
BfrBI ATG'CAT

Cuts at:      0    2122    3999 
   Size:       2122    1877
 
BglI GCCn_nnn'nGGC

Cuts at:      0      54    3999 
   Size:         54    3945
 
BglII A'GATC_T

Cuts at:      0    2379    3391    3999 
   Size:       2379    1012     608

  Fragments arranged by size: 

                2379    1012     608
 
BlpI GC'TnA_GC

Cuts at:      0     185    3999 
   Size:        185    3814
 
Bme1580I G_kGCm'C

Cuts at:      0    2309    3999 
   Size:       2309    1690
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     310     787    2094    3999 
   Size:        310     477    1307    1905

  Fragments arranged by size: 

                1905    1307     477     310
 
BmtI G_CTAG'C

Cuts at:      0    1451    3999 
   Size:       1451    2548
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1758    2288    3999 
   Size:       1758     530    1711

  Fragments arranged by size: 

                1758    1711     530
 
Bpu10I CC'TnA_GC

Cuts at:      0    1958    3062    3999 
   Size:       1958    1104     937

  Fragments arranged by size: 

                1958    1104     937
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1895    2028    2635    3327    3663    3999 
   Size:       1895     133     607     692     336     336

  Fragments arranged by size: 

                1895     692     607     336     336     133
 
BsaAI yAC'GTr

Cuts at:      0    1354    1841    3999 
   Size:       1354     487    2158

  Fragments arranged by size: 

                2158    1354     487
 
BsaBI GATnn'nnATC

Cuts at:      0    2123    3320    3999 
   Size:       2123    1197     679

  Fragments arranged by size: 

                2123    1197     679
 
BsaWI w'CCGG_w

Cuts at:      0    1637    3599    3999 
   Size:       1637    1962     400

  Fragments arranged by size: 

                1962    1637     400
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    3680    3710    3759    3777    3789    3807    3999 
   Size:       3680      30      49      18      12      18     192

  Fragments arranged by size: 

                3680     192      49      30      18      18      12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1705    3440    3767    3770    3785    3999 
   Size:       1705    1735     327       3      15     214

  Fragments arranged by size: 

                1735    1705     327     214      15       3
 
BseYI C'CCAG_C

Cuts at:      0    3486    3537    3862    3999 
   Size:       3486      51     325     137

  Fragments arranged by size: 

                3486     325     137      51
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2033    3218    3999 
   Size:       2033    1185     781

  Fragments arranged by size: 

                2033    1185     781
 
BsiHKAI G_wGCw'C

Cuts at:      0    2096    3941    3999 
   Size:       2096    1845      58

  Fragments arranged by size: 

                2096    1845      58
 
BsmI GAATG_Cn'

Cuts at:      0    3990    3999 
   Size:       3990       9
 
Bsp1286I G_dGCh'C

Cuts at:      0     686    2096    2309    3334    3941    3999 
   Size:        686    1410     213    1025     607      58

  Fragments arranged by size: 

                1410    1025     686     607     213      58
 
BspHI T'CATG_A

Cuts at:      0     921    2712    3999 
   Size:        921    1791    1287

  Fragments arranged by size: 

                1791    1287     921
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2333    3999 
   Size:       2333    1666
 
BsrDI GCAATG_nn'

Cuts at:      0     500    1458    2180    3354    3741    3857    3999 
   Size:        500     958     722    1174     387     116     142

  Fragments arranged by size: 

                1174     958     722     500     387     142     116
 
BsrGI T'GTAC_A

Cuts at:      0    2394    3999 
   Size:       2394    1605
 
BssSI C'ACGA_G

Cuts at:      0    3647    3999 
   Size:       3647     352
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      15     499    3999 
   Size:         15     484    3500

  Fragments arranged by size: 

                3500     484      15
 
BstYI r'GATC_y

Cuts at:      0    1857    1894    2379    2909    3200    3391    3999 
   Size:       1857      37     485     530     291     191     608

  Fragments arranged by size: 

                1857     608     530     485     291     191      37
 
Bsu36I CC'TnA_GG

Cuts at:      0     585    2834    3999 
   Size:        585    2249    1165

  Fragments arranged by size: 

                2249    1165     585
 
BtgI C'CryG_G

Cuts at:      0    3381    3729    3999 
   Size:       3381     348     270

  Fragments arranged by size: 

                3381     348     270
 
BtsI GCAGTG_nn'

Cuts at:      0    3312    3999 
   Size:       3312     687
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3959    3994    3999 
   Size:       3959      35       5

  Fragments arranged by size: 

                3959      35       5
 
DraI TTT'AAA

Cuts at:      0     425    1118    1553    3999 
   Size:        425     693     435    2446

  Fragments arranged by size: 

                2446     693     435     425
 
DraIII CAC_nnn'GTG

Cuts at:      0    2498    2776    3999 
   Size:       2498     278    1223

  Fragments arranged by size: 

                2498    1223     278
 
EarI CTCTTCn'nnn_

Cuts at:      0      32     137     566    1589    1676    1742    1760    3999 
   Size:         32     105     429    1023      87      66      18    2239

  Fragments arranged by size: 

                2239    1023     429     105      87      66      32      18
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3257    3999 
   Size:       3257     742
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     185    1613    1766    3999 
   Size:        118      67    1428     153    2233

  Fragments arranged by size: 

                2233    1428     153     118      67
 
EcoO109I rG'GnC_Cy

Cuts at:      0     348    2838    3999 
   Size:        348    2490    1161

  Fragments arranged by size: 

                2490    1161     348
 
EcoRI G'AATT_C

Cuts at:      0    1790    2634    3999 
   Size:       1790     844    1365

  Fragments arranged by size: 

                1790    1365     844
 
EcoRV GAT'ATC

Cuts at:      0    1625    2958    3999 
   Size:       1625    1333    1041

  Fragments arranged by size: 

                1625    1333    1041
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     320    1363    3413    3865    3999 
   Size:        320    1043    2050     452     134

  Fragments arranged by size: 

                2050    1043     452     320     134
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     256     288    2712    2744    3589    3621    3661    3693 
   Size:        256      32    2424      32     845      32      40      32

Cuts at:   3693    3999 
   Size:        306

  Fragments arranged by size: 

                2424     845     306     256      40      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    3260    3999 
   Size:       3260     739
 
HpaI GTT'AAC

Cuts at:      0    3260    3999 
   Size:       3260     739
 
KpnI G_GTAC'C

Cuts at:      0    3146    3999 
   Size:       3146     853
 
MfeI C'AATT_G

Cuts at:      0     242    1605    3999 
   Size:        242    1363    2394

  Fragments arranged by size: 

                2394    1363     242
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2394    3723    3959    3999 
   Size:       2394    1329     236      40

  Fragments arranged by size: 

                2394    1329     236      40
 
MslI CAynn'nnrTG

Cuts at:      0     533    2102    3734    3962    3999 
   Size:        533    1569    1632     228      37

  Fragments arranged by size: 

                1632    1569     533     228      37
 
MspA1I CmG'CkG

Cuts at:      0      15    1591    3999 
   Size:         15    1576    2408

  Fragments arranged by size: 

                2408    1576      15
 
NcoI C'CATG_G

Cuts at:      0    3381    3729    3999 
   Size:       3381     348     270

  Fragments arranged by size: 

                3381     348     270
 
NdeI CA'TA_TG

Cuts at:      0     882    1847    3999 
   Size:        882     965    2152

  Fragments arranged by size: 

                2152     965     882
 
NheI G'CTAG_C

Cuts at:      0    1447    3999 
   Size:       1447    2552
 
NsiI A_TGCA'T

Cuts at:      0    2124    3999 
   Size:       2124    1875
 
NspI r_CATG'y

Cuts at:      0    1214    1537    1776    3999 
   Size:       1214     323     239    2223

  Fragments arranged by size: 

                2223    1214     323     239
 
PmlI CAC'GTG

Cuts at:      0    1354    3999 
   Size:       1354    2645
 
PpuMI rG'GwC_Cy

Cuts at:      0     348    2838    3999 
   Size:        348    2490    1161

  Fragments arranged by size: 

                2490    1161     348
 
PsiI TTA'TAA

Cuts at:      0     359     667    1333    1930    2866    3999 
   Size:        359     308     666     597     936    1133

  Fragments arranged by size: 

                1133     936     666     597     359     308
 
PstI C_TGCA'G

Cuts at:      0     417    3170    3999 
   Size:        417    2753     829

  Fragments arranged by size: 

                2753     829     417
 
PvuII CAG'CTG

Cuts at:      0      15    1591    3999 
   Size:         15    1576    2408

  Fragments arranged by size: 

                2408    1576      15
 
SapI GCTCTTCn'nnn_

Cuts at:      0     137     566    3999 
   Size:        137     429    3433

  Fragments arranged by size: 

                3433     429     137
 
SexAI A'CCwGG_T

Cuts at:      0     371     912    2241    3138    3999 
   Size:        371     541    1329     897     861

  Fragments arranged by size: 

                1329     897     861     541     371
 
SfcI C'TryA_G

Cuts at:      0     413    1675    1985    3166    3999 
   Size:        413    1262     310    1181     833

  Fragments arranged by size: 

                1262    1181     833     413     310
 
SmlI C'TyrA_G

Cuts at:      0     112     896    1910    2007    2614    3342    3678    3999 
   Size:        112     784    1014      97     607     728     336     321

  Fragments arranged by size: 

                1014     784     728     607     336     321     112      97
            
 
SnaBI TAC'GTA

Cuts at:      0    1841    3999 
   Size:       1841    2158
 
SpeI A'CTAG_T

Cuts at:      0     831    3999 
   Size:        831    3168
 
SphI G_CATG'C

Cuts at:      0    1537    3999 
   Size:       1537    2462
 
SspI AAT'ATT

Cuts at:      0    1284    1755    2253    2743    3999 
   Size:       1284     471     498     490    1256

  Fragments arranged by size: 

                1284    1256     498     490     471
 
StyI C'CwwG_G

Cuts at:      0     351    1781    3178    3381    3550    3729    3999 
   Size:        351    1430    1397     203     169     179     270

  Fragments arranged by size: 

                1430    1397     351     270     203     179     169
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2354    3962    3999 
   Size:       2354    1608      37

  Fragments arranged by size: 

                2354    1608      37
 
TatI w'GTAC_w

Cuts at:      0    1642    2394    3223    3999 
   Size:       1642     752     829     776

  Fragments arranged by size: 

                1642     829     776     752
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      53      85    3680    3712    3999 
   Size:         53      32    3595      32     287

  Fragments arranged by size: 

                3595     287      53      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2243    3999 
   Size:       2243    1756
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     248    1026    3999 
   Size:        248     778    2973

  Fragments arranged by size: 

                2973     778     248
 
XhoI C'TCGA_G

Cuts at:      0     112     896    3999 
   Size:        112     784    3103

  Fragments arranged by size: 

                3103     784     112
 
XmnI GAAnn'nnTTC

Cuts at:      0     198     218     326    1001    3999 
   Size:        198      20     108     675    2998

  Fragments arranged by size: 

                2998     675     198     108      20
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII    AlwNI     ApoI     AseI     AvaI    AvrII
    BamHI     BanI    BanII     BbsI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspHI    BspMI    BsrDI    BsrGI    BssSI   BstAPI    BstYI   Bsu36I
     BtgI     BtsI    CspCI     DraI   DraIII     EarI     EciI  Eco57MI
 EcoO109I    EcoRI    EcoRV     FalI    Hin4I   HincII     HpaI     KpnI
     MfeI     MmeI     MslI   MspA1I     NcoI     NdeI     NheI     NsiI
     NspI     PmlI    PpuMI     PsiI     PstI    PvuII     SapI    SexAI
     SfcI     SmlI    SnaBI     SpeI     SphI     SspI     StyI    TaqII
     TatI     TstI  Tth111I     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     BaeI
     BbeI    BbvCI     BcgI    BciVI     BclI    BmgBI     BpmI     BsaI
    BsaHI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrFI   BssHII
    BstBI   BstEII    BstXI  BstZ17I    BtgZI     ClaI     DrdI     EaeI
     EagI  EcoICRI    EcoNI     FseI     FspI    FspAI    HaeII  HindIII
     KasI     MluI     MscI     NaeI     NarI   NgoMIV     NotI     NruI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PshAI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SrfI     StuI     SwaI     XbaI     XmaI     ZraI
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