Restriction map for
pFN21AB7273
(Linear) (Six-base) MAPSORT of: KIBB7273.seq Check: 4168 from: 1 to: 3996
KIBB7273.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AatII G_ACGT'C
Cuts at: 0 1188 3996
Size: 1188 2808
AccI GT'mk_AC
Cuts at: 0 1188 2642 3996
Size: 1188 1454 1354
Fragments arranged by size:
1454 1354 1188
Acc65I G'GTAC_C
Cuts at: 0 3807 3996
Size: 3807 189
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 1607 1730 2827 3275 3685 3996
Size: 15 1592 123 1097 448 410 311
Fragments arranged by size:
1592 1097 448 410 311 123 15
AflII C'TTAA_G
Cuts at: 0 2749 3996
Size: 2749 1247
AflIII A'CryG_T
Cuts at: 0 521 3760 3996
Size: 521 3239 236
Fragments arranged by size:
3239 521 236
AhdI GACnn_n'nnGTC
Cuts at: 0 67 3996
Size: 67 3929
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1544 3996
Size: 1544 2452
AlwNI CAG_nnn'CTG
Cuts at: 0 3283 3332 3996
Size: 3283 49 664
Fragments arranged by size:
3283 664 49
ApoI r'AATT_y
Cuts at: 0 1160 1265 1321 1471 1493 1940 2442 2451
Size: 1160 105 56 150 22 447 502 9
Cuts at: 2451 2523 2564 2628 2690 2903 3480 3768 3888
Size: 72 41 64 62 213 577 288 120
Cuts at: 3888 3996
Size: 108
Fragments arranged by size:
1160 577 502 447 288 213 150 120
108 105 72 64 62 56 41 22
9
AseI AT'TA_AT
Cuts at: 0 1819 3996
Size: 1819 2177
AvrII C'CTAG_G
Cuts at: 0 3076 3996
Size: 3076 920
BanI G'GyrC_C
Cuts at: 0 9 1026 1354 3807 3996
Size: 9 1017 328 2453 189
Fragments arranged by size:
2453 1017 328 189 9
BanII G_rGCy'C
Cuts at: 0 3428 3996
Size: 3428 568
BbeI G_GCGC'C
Cuts at: 0 13 3996
Size: 13 3983
BbsI GAAGACnn'nnnn_
Cuts at: 0 232 791 1103 1766 2815 3996
Size: 232 559 312 663 1049 1181
Fragments arranged by size:
1181 1049 663 559 312 232
BciVI GTATCCnnnnn_n'
Cuts at: 0 387 1554 3996
Size: 387 1167 2442
Fragments arranged by size:
2442 1167 387
BclI T'GATC_A
Cuts at: 0 528 656 1397 3465 3996
Size: 528 128 741 2068 531
Fragments arranged by size:
2068 741 531 528 128
BfrBI ATG'CAT
Cuts at: 0 179 1531 2507 3996
Size: 179 1352 976 1489
Fragments arranged by size:
1489 1352 976 179
BglI GCCn_nnn'nGGC
Cuts at: 0 3134 3996
Size: 3134 862
BlpI GC'TnA_GC
Cuts at: 0 1915 3996
Size: 1915 2081
BmgBI CAC'GTC
Cuts at: 0 1015 2350 3996
Size: 1015 1335 1646
Fragments arranged by size:
1646 1335 1015
BmrI ACTGGGnnnn_n'
Cuts at: 0 3724 3996
Size: 3724 272
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 426 3554 3996
Size: 426 3128 442
Fragments arranged by size:
3128 442 426
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1214 2918 3341 3653 3845 3996
Size: 1214 1704 423 312 192 151
Fragments arranged by size:
1704 1214 423 312 192 151
Bpu10I CC'TnA_GC
Cuts at: 0 825 2222 3996
Size: 825 1397 1774
Fragments arranged by size:
1774 1397 825
BsaI GGTCTCn'nnnn_
Cuts at: 0 75 3996
Size: 75 3921
BsaAI yAC'GTr
Cuts at: 0 3761 3996
Size: 3761 235
BsaBI GATnn'nnATC
Cuts at: 0 3317 3509 3996
Size: 3317 192 487
Fragments arranged by size:
3317 487 192
BsaHI Gr'CG_yC
Cuts at: 0 10 1185 3996
Size: 10 1175 2811
Fragments arranged by size:
2811 1175 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1187 1217 1793 1823 3783 3813 3996
Size: 1187 30 576 30 1960 30 183
Fragments arranged by size:
1960 1187 576 183 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 226 2889 2970 3428 3431 3440 3530
Size: 52 174 2663 81 458 3 9 90
Cuts at: 3530 3533 3773 3996
Size: 3 240 223
Fragments arranged by size:
2663 458 240 223 174 90 81 52
9 3 3
BseYI C'CCAG_C
Cuts at: 0 185 3996
Size: 185 3811
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1469 2027 2642 3996
Size: 1469 558 615 1354
Fragments arranged by size:
1469 1354 615 558
BsiHKAI G_wGCw'C
Cuts at: 0 1523 3830 3996
Size: 1523 2307 166
Fragments arranged by size:
2307 1523 166
Bsp1286I G_dGCh'C
Cuts at: 0 1523 3428 3830 3996
Size: 1523 1905 402 166
Fragments arranged by size:
1905 1523 402 166
BspHI T'CATG_A
Cuts at: 0 915 1133 1478 2321 3468 3996
Size: 915 218 345 843 1147 528
Fragments arranged by size:
1147 915 843 528 345 218
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 954 3996
Size: 954 3042
BsrDI GCAATG_nn'
Cuts at: 0 257 971 3996
Size: 257 714 3025
Fragments arranged by size:
3025 714 257
BsrGI T'GTAC_A
Cuts at: 0 3956 3996
Size: 3956 40
BstAPI GCAn_nnn'nTGC
Cuts at: 0 274 3996
Size: 274 3722
BstEII G'GTnAC_C
Cuts at: 0 3160 3996
Size: 3160 836
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2279 3996
Size: 2279 1717
BstYI r'GATC_y
Cuts at: 0 1197 1851 2057 3318 3627 3996
Size: 1197 654 206 1261 309 369
Fragments arranged by size:
1261 1197 654 369 309 206
BstZ17I GTA'TAC
Cuts at: 0 2643 3996
Size: 2643 1353
Bsu36I CC'TnA_GG
Cuts at: 0 3144 3996
Size: 3144 852
BtgI C'CryG_G
Cuts at: 0 2981 3996
Size: 2981 1015
BtsI GCAGTG_nn'
Cuts at: 0 2668 3996
Size: 2668 1328
ClaI AT'CG_AT
Cuts at: 0 1280 3996
Size: 1280 2716
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1892 1927 3996
Size: 1892 35 2069
Fragments arranged by size:
2069 1892 35
DraI TTT'AAA
Cuts at: 0 425 987 2730 2837 3996
Size: 425 562 1743 107 1159
Fragments arranged by size:
1743 1159 562 425 107
DraIII CAC_nnn'GTG
Cuts at: 0 2770 3996
Size: 2770 1226
EaeI y'GGCC_r
Cuts at: 0 938 1419 3996
Size: 938 481 2577
Fragments arranged by size:
2577 938 481
EarI CTCTTCn'nnn_
Cuts at: 0 119 140 851 1628 3416 3449 3638 3996
Size: 119 21 711 777 1788 33 189 358
Fragments arranged by size:
1788 777 711 358 189 119 33 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3602 3996
Size: 3602 394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 1214 1607 1730 2827 2918 3275 3341
Size: 15 1199 393 123 1097 91 357 66
Cuts at: 3341 3653 3685 3845 3996
Size: 312 32 160 151
Fragments arranged by size:
1199 1097 393 357 312 160 151 123
91 66 32 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 82 3122 3801 3996
Size: 82 3040 679 195
Fragments arranged by size:
3040 679 195 82
EcoRI G'AATT_C
Cuts at: 0 1321 3888 3996
Size: 1321 2567 108
Fragments arranged by size:
2567 1321 108
EcoRV GAT'ATC
Cuts at: 0 717 3996
Size: 717 3279
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 700 3402 3996
Size: 700 2702 594
Fragments arranged by size:
2702 700 594
HaeII r_GCGC'y
Cuts at: 0 13 3996
Size: 13 3983
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 53 85 219 251 256 288 2316 2348
Size: 53 32 134 32 5 32 2028 32
Cuts at: 2348 3214 3246 3423 3455 3996
Size: 866 32 177 32 541
Fragments arranged by size:
2028 866 541 177 134 53 32 32
32 32 32 32 5
HincII GTy'rAC
Cuts at: 0 1189 1832 3996
Size: 1189 643 2164
Fragments arranged by size:
2164 1189 643
HindIII A'AGCT_T
Cuts at: 0 3198 3996
Size: 3198 798
HpaI GTT'AAC
Cuts at: 0 1832 3996
Size: 1832 2164
KasI G'GCGC_C
Cuts at: 0 9 3996
Size: 9 3987
KpnI G_GTAC'C
Cuts at: 0 3811 3996
Size: 3811 185
MfeI C'AATT_G
Cuts at: 0 242 1069 2189 2273 3996
Size: 242 827 1120 84 1723
Fragments arranged by size:
1723 1120 827 242 84
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2064 3187 3996
Size: 2064 1123 809
Fragments arranged by size:
2064 1123 809
MscI TGG'CCA
Cuts at: 0 940 1421 3996
Size: 940 481 2575
Fragments arranged by size:
2575 940 481
MslI CAynn'nnrTG
Cuts at: 0 487 664 1526 1844 2096 3996
Size: 487 177 862 318 252 1900
Fragments arranged by size:
1900 862 487 318 252 177
MspA1I CmG'CkG
Cuts at: 0 162 415 836 2355 3996
Size: 162 253 421 1519 1641
Fragments arranged by size:
1641 1519 421 253 162
NarI GG'CG_CC
Cuts at: 0 10 3996
Size: 10 3986
NcoI C'CATG_G
Cuts at: 0 2981 3996
Size: 2981 1015
NdeI CA'TA_TG
Cuts at: 0 1841 3996
Size: 1841 2155
NsiI A_TGCA'T
Cuts at: 0 181 1533 2509 3996
Size: 181 1352 976 1487
Fragments arranged by size:
1487 1352 976 181
NspI r_CATG'y
Cuts at: 0 525 1082 1531 3996
Size: 525 557 449 2465
Fragments arranged by size:
2465 557 525 449
PciI A'CATG_T
Cuts at: 0 521 3996
Size: 521 3475
PfoI T'CCnGG_A
Cuts at: 0 3105 3996
Size: 3105 891
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3554 3586 3996
Size: 3554 32 410
Fragments arranged by size:
3554 410 32
PsiI TTA'TAA
Cuts at: 0 3682 3996
Size: 3682 314
PstI C_TGCA'G
Cuts at: 0 951 2675 3996
Size: 951 1724 1321
Fragments arranged by size:
1724 1321 951
PvuII CAG'CTG
Cuts at: 0 162 415 836 2355 3996
Size: 162 253 421 1519 1641
Fragments arranged by size:
1641 1519 421 253 162
SalI G'TCGA_C
Cuts at: 0 1187 3996
Size: 1187 2809
SapI GCTCTTCn'nnn_
Cuts at: 0 140 851 3416 3996
Size: 140 711 2565 580
Fragments arranged by size:
2565 711 580 140
ScaI AGT'ACT
Cuts at: 0 1638 2546 3996
Size: 1638 908 1450
Fragments arranged by size:
1638 1450 908
SfcI C'TryA_G
Cuts at: 0 278 947 1808 2671 3996
Size: 278 669 861 863 1325
Fragments arranged by size:
1325 863 861 669 278
SfoI GGC'GCC
Cuts at: 0 11 3996
Size: 11 3985
SmlI C'TyrA_G
Cuts at: 0 2749 3996
Size: 2749 1247
SphI G_CATG'C
Cuts at: 0 1531 3996
Size: 1531 2465
SspI AAT'ATT
Cuts at: 0 1568 2247 2802 3996
Size: 1568 679 555 1194
Fragments arranged by size:
1568 1194 679 555
StuI AGG'CCT
Cuts at: 0 3117 3996
Size: 3117 879
StyI C'CwwG_G
Cuts at: 0 190 1143 2981 3076 3485 3802 3880 3996
Size: 190 953 1838 95 409 317 78 116
Fragments arranged by size:
1838 953 409 317 190 116 95 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 254 1172 2827 3179 3996
Size: 254 918 1655 352 817
Fragments arranged by size:
1655 918 817 352 254
TatI w'GTAC_w
Cuts at: 0 396 1636 2046 2544 3956 3996
Size: 396 1240 410 498 1412 40
Fragments arranged by size:
1412 1240 498 410 396 40
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 952 984 1796 1828 3783 3815 3996
Size: 952 32 812 32 1955 32 181
Fragments arranged by size:
1955 952 812 181 32 32 32
XbaI T'CTAG_A
Cuts at: 0 1641 3996
Size: 1641 2355
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 248 2276 3996
Size: 248 2028 1720
Fragments arranged by size:
2028 1720 248
XmnI GAAnn'nnTTC
Cuts at: 0 680 2593 3996
Size: 680 1913 1403
Fragments arranged by size:
1913 1403 680
ZraI GAC'GTC
Cuts at: 0 1186 3996
Size: 1186 2810
Enzymes that do cut:
AatII AccI Acc65I AcuI AflII AflIII AhdI AlfI
AlwNI ApoI AseI AvrII BanI BanII BbeI BbsI
BciVI BclI BfrBI BglI BlpI BmgBI BmrI BplI
BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrGI
BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI
ClaI CspCI DraI DraIII EaeI EarI EciI Eco57MI
EcoNI EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MmeI MscI MslI MspA1I
NarI NcoI NdeI NsiI NspI PciI PfoI PpiI
PsiI PstI PvuII SalI SapI ScaI SfcI SfoI
SmlI SphI SspI StuI StyI TaqII TatI TstI
XbaI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AgeI AleI AloI ApaI ApaLI
AscI AsiSI AvaI BaeI BamHI BbvCI BcgI BglII
Bme1580I BmtI BpuEI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BsrBI BsrFI BssHII BssSI BstBI BtgZI DrdI
EagI EcoICRI EcoO109I FseI FspI FspAI MluI NaeI
NgoMIV NheI NotI NruI PacI PasI PflMI PmeI
PmlI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SanDI SbfI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SwaI Tth111I XhoI XmaI