Restriction map for
pFN21AB7271
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BamHI BbrPI BbvCI BcgI BciVI
BclI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrDI BssHII Bst1107I
BstAPI BstBI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI HgaI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MluI MroI
MunI NcoI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SspI StuI SwaI Tth111I Van91I
XbaI XhoI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AccII 405 CGCG CG^CG
AcyI 1370 GGCGCC G[GA]^CG[CT]C
AfaI 347 GTAC GT^AC
AflII 1180 CTTAAG C^TTAAG
Asp700I 1212 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1449 CCCGGG C^[CT]CG[GA]G
AviII 1340 TGCGCA TGC^GCA
BalI 383 TGGCCA TGG^CCA
BanI 1370 GGCGCC G^G[CT][GA]CC
BbeI 1370 GGCGCC GGCGC^C
BbsI 1155 GAAGAC GAAGAC(2/6)
BfrI 1180 CTTAAG C^TTAAG
BglI 376 GCCGTAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1155 GAAGAC GAAGAC(2/6)
BsaHI 1370 GGCGCC G[GA]^CG[CT]C
BsaI 891 GAGACC GGTCTC(1/5)
BsoBI 1449 CCCGGG C^[CT]CG[GA]G
Bsp1407I 346 TGTACA T^GTACA
BsrBI 396 GAGCGG CCGCTC(-3/-3)
BsrFI 533 GCCGGC [GA]^CCGG[CT]
BsrGI 346 TGTACA T^GTACA
BssSI 289 CTCGTG CACGAG(-5/-1)
BstEII 139 GGTGACC G^GT[ACGT]ACC
BstPI 139 GGTGACC G^GT[ACGT]ACC
BstUI 405 CGCG CG^CG
BstXI 1016 CCAATGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 533 GCCGGC [GA]^CCGG[CT]
DraII 939 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 353 CCACGG C^C[GA][CT]GG
EaeI 383 TGGCCA [CT]^GGCC[GA]
EarI 559 GAAGAG CTCTTC(1/4)
EcoO109I 939 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 139 GGTGACC G^GT[ACGT]ACC
EcoT14I 458 CCAAGG C^C[AT][AT]GG
FauI 398 GCGGG CCCGC(4/6)
FspI 1340 TGCGCA TGC^GCA
Hin1I 1370 GGCGCC G[GA]^CG[CT]C
KasI 1370 GGCGCC G^GCGCC
Ksp632I 559 GAAGAG CTCTTC(1/4)
MaeII 1332 ACGT A^CGT
MluNI 383 TGGCCA TGG^CCA
MscI 383 TGGCCA TGG^CCA
MseI 1181 TTAA T^TAA
MspA1I 1227 CAGCTG C[AC]G^C[GT]G
MvnI 405 CGCG CG^CG
NaeI 533 GCCGGC GCC^GGC
NarI 1370 GGCGCC GG^CGCC
NgoMIV 533 GCCGGC G^CCGGC
PpuMI 939 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 1227 CAGCTG CAG^CTG
RsaI 347 GTAC GT^AC
SapI 559 GAAGAGC GCTCTTC(1/4)
SbfI 449 CCTGCAGG CCTGCA^GG
SfoI 1370 GGCGCC GGC^GCC
SmaI 1449 CCCGGG CCC^GGG
SmlI 1180 CTTAAG C^T[CT][GA]AG
Sse8387I 449 CCTGCAGG CCTGCA^GG
SspBI 346 TGTACA T^GTACA
StyI 458 CCAAGG C^C[AT][AT]GG
Tru9I 1181 TTAA T^TAA
Tsp509I 736 AATT ^AATT
XcmI 197 CCACTGTGCATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1449 CCCGGG C^CCGGG
XmaI 1449 CCCGGG C^CCGGG
XmnI 1212 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 51 GTCTAC GT^[AC][GT]AC
1105 GTAGAC
AlwNI 565 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1475 CAGCCTCTG
AocI 952 CCTCAGG CC^T[ACGT]AGG
1099 CCTCAGG
AspHI 111 GAGCTC G[AT]GC[AT]^C
389 GAGCTC
AvaII 940 GGACC G^G[AT]CC
972 GGTCC
BseRI 269 CTCCTC GAGGAG(10/8)
272 CTCCTC
BsgI 710 GTGCAG GTGCAG(16/14)
1010 GTGCAG
BsiHKAI 111 GAGCTC G[AT]GC[AT]^C
389 GAGCTC
BspMI 749 ACCTGC ACCTGC(4/8)
942 ACCTGC
BstYI 127 AGATCC [GA]^GATC[CT]
1391 AGATCC
Bsu36I 952 CCTCAGG CC^T[ACGT]AGG
1099 CCTCAGG
Eco81I 952 CCTCAGG CC^T[ACGT]AGG
1099 CCTCAGG
EcoNI 900 CCTAGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
975 CCTGCCCCAGG
HaeII 918 AGCGCC [GA]GCGC^[CT]
1370 GGCGCC
MflI 127 AGATCC [GA]^GATC[CT]
1391 AGATCC
MslI 195 CACCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
205 CATATGGATG
NdeI 6 CATATG CA^TATG
205 CATATG
SacI 111 GAGCTC GAGCT^C
389 GAGCTC
SfaNI 211 GATGC GCATC(5/9)
62 GCATC
SphI 1362 GCATGC GCATG^C
1469 GCATGC
TfiI 334 GATTC G^A[AT]TC
1430 GAATC
XhoII 127 AGATCC [GA]^GATC[CT]
1391 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI AlwI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
Sau3AI Sau96I ScrFI SfcI TaqI
TseI Tsp45I TspRI TthHB8I