(Circular) (Six-base) MAPSORT of: pFN21AB7271.seq Check: 1089 from: 1 to: 6190 pFN21AB7271 6190 bp With 182 enzymes: SgfI * February 27, 2009 14:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4000 278 Size: 53 83 186 936 2464 2468 Fragments arranged by size: 2468 2464 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2057 3111 3533 1937 Size: 120 1054 422 4594 Fragments arranged by size: 4594 1054 422 120 Acc65I G'GTAC_C Cuts at: 3511 3511 Size: 6190 AclI AA'CG_TT Cuts at: 1563 4317 4690 1563 Size: 2754 373 3063 Fragments arranged by size: 3063 2754 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4253 5637 5905 1632 Size: 2621 1384 268 1917 Fragments arranged by size: 2621 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 3185 820 Size: 197 2168 3825 Fragments arranged by size: 3825 2168 197 AflIII A'CryG_T Cuts at: 1203 5089 6066 1203 Size: 3886 977 1327 Fragments arranged by size: 3886 1327 977 AgeI A'CCGG_T Cuts at: 5021 5021 Size: 6190 AhdI GACnn_n'nnGTC Cuts at: 4919 4919 Size: 6190 AleI CACnn'nnGTG Cuts at: 1166 2204 1166 Size: 1038 5152 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2232 1184 Size: 1048 5142 AlwNI CAG_nnn'CTG Cuts at: 2575 3485 3933 5505 2575 Size: 910 448 1572 3260 Fragments arranged by size: 3260 1572 910 448 ApaLI G'TGCA_C Cuts at: 4247 5403 4247 Size: 1156 5034 ApoI r'AATT_y Cuts at: 1493 3499 3725 3877 5986 1493 Size: 2006 226 152 2109 1697 Fragments arranged by size: 2109 2006 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4744 160 Size: 872 3712 1606 Fragments arranged by size: 3712 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6190 AvaI C'yCGr_G Cuts at: 1870 1943 3454 3515 1870 Size: 73 1511 61 4545 Fragments arranged by size: 4545 1511 73 61 BamHI G'GATC_C Cuts at: 3520 3887 3520 Size: 367 5823 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3375 3511 4966 618 Size: 325 217 627 1588 136 1455 1842 Fragments arranged by size: 1842 1588 1455 627 325 217 136 BanII G_rGCy'C Cuts at: 1391 2120 2288 2398 2501 2995 3455 3464 3509 Size: 729 168 110 103 494 460 9 45 Cuts at: 3509 1391 Size: 4072 Fragments arranged by size: 4072 729 494 460 168 110 103 45 9 BbeI G_GCGC'C Cuts at: 3379 3379 Size: 6190 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3167 928 Size: 971 1268 3951 Fragments arranged by size: 3951 1268 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4381 4415 1903 Size: 34 2444 34 3678 Fragments arranged by size: 3678 2444 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4082 5292 4082 Size: 1210 4980 BclI T'GATC_A Cuts at: 1548 1548 Size: 6190 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2387 4801 136 Size: 107 122 71 631 605 715 2414 1525 Fragments arranged by size: 2414 1525 715 631 605 122 107 71 BglII A'GATC_T Cuts at: 6185 6185 Size: 6190 BlpI GC'TnA_GC Cuts at: 3607 3607 Size: 6190 Bme1580I G_kGCm'C Cuts at: 1790 2445 3323 4251 5407 1790 Size: 655 878 928 1156 2573 Fragments arranged by size: 2573 1156 928 878 655 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6190 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2499 2869 3921 Size: 416 53 83 399 24 1082 370 1052 Cuts at: 3921 4879 442 Size: 958 1753 Fragments arranged by size: 1753 1082 1052 958 416 399 370 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6190 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2048 2354 2514 2904 3054 4850 1239 Size: 809 306 160 390 150 1796 2579 Fragments arranged by size: 2579 1796 809 390 306 160 150 Bpu10I CC'TnA_GC Cuts at: 2910 2910 Size: 6190 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4315 5180 5478 4315 Size: 865 298 5027 Fragments arranged by size: 5027 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2890 4853 882 Size: 617 1391 1963 2219 Fragments arranged by size: 2219 1963 1391 617 BsaAI yAC'GTr Cuts at: 493 1204 3981 493 Size: 711 2777 2702 Fragments arranged by size: 2777 2702 711 BsaBI GATnn'nnATC Cuts at: 6184 6184 Size: 6190 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3376 3997 4379 5014 Size: 53 83 186 936 1843 621 382 635 Cuts at: 5014 275 Size: 1451 Fragments arranged by size: 1843 1451 936 635 621 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4623 5021 5295 5442 5853 4623 Size: 398 274 147 411 4960 Fragments arranged by size: 4960 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3296 3326 1178 Size: 30 165 30 1893 30 4042 Fragments arranged by size: 4042 1893 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2265 2268 1186 Size: 1079 3 5108 Fragments arranged by size: 5108 1079 3 BseYI C'CCAG_C Cuts at: 2425 2444 3234 5393 5889 2425 Size: 19 790 2159 496 2726 Fragments arranged by size: 2726 2159 790 496 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2736 3036 1163 Size: 573 1000 300 4317 Fragments arranged by size: 4317 1000 573 300 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3640 4401 5429 1540 Size: 275 186 1639 761 1028 2301 Fragments arranged by size: 2301 1639 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2120 2398 4251 4336 5407 2120 Size: 278 1853 85 1071 2903 Fragments arranged by size: 2903 1853 1071 278 85 BsmI GAATG_Cn' Cuts at: 3701 3794 3701 Size: 93 6097 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2120 2288 2398 2445 2501 2995 3323 Size: 399 330 168 110 47 56 494 328 Cuts at: 3323 3455 3464 3509 4251 4336 5407 1391 Size: 132 9 45 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 494 399 330 328 168 132 110 85 56 47 45 9 BspEI T'CCGG_A Cuts at: 5853 5853 Size: 6190 BspHI T'CATG_A Cuts at: 4079 5809 4079 Size: 1730 4460 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2763 2956 3545 844 Size: 1919 193 589 3489 Fragments arranged by size: 3489 1919 589 193 BsrBI CCG'CTC Cuts at: 1394 1823 2403 4077 1394 Size: 429 580 1674 3507 Fragments arranged by size: 3507 1674 580 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4685 4859 65 Size: 1183 3437 174 1396 Fragments arranged by size: 3437 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2538 4834 5021 1700 Size: 110 728 2296 187 2869 Fragments arranged by size: 2869 2296 728 187 110 BsrGI T'GTAC_A Cuts at: 96 2351 96 Size: 2255 3935 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6190 BssSI C'ACGA_G Cuts at: 2294 4250 5262 2294 Size: 1956 1012 3222 Fragments arranged by size: 3222 1956 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6190 BstBI TT'CG_AA Cuts at: 4994 4994 Size: 6190 BstEII G'GTnAC_C Cuts at: 1184 2144 1184 Size: 960 5230 BstXI CCAn_nnnn'nTGG Cuts at: 3028 3028 Size: 6190 BstYI r'GATC_y Cuts at: 1972 2132 3396 3520 3887 4272 4289 5741 5827 Size: 160 1264 124 367 385 17 1452 86 Cuts at: 5827 5839 6185 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1264 385 367 346 160 124 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2958 3105 2958 Size: 147 6043 BtgI C'CryG_G Cuts at: 513 1065 2004 2358 513 Size: 552 939 354 4345 Fragments arranged by size: 4345 939 552 354 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5451 Fragments arranged by size: 5451 683 56 BtsI GCAGTG_nn' Cuts at: 3355 3706 4500 4520 3355 Size: 351 794 20 5025 Fragments arranged by size: 5025 794 351 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6190 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2650 2685 529 Size: 35 2086 35 4034 Fragments arranged by size: 4034 2086 35 35 DraI TTT'AAA Cuts at: 3494 3494 Size: 6190 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5197 809 Size: 507 3881 1802 Fragments arranged by size: 3881 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2388 3637 4526 Size: 54 1425 50 275 18 558 1249 889 Cuts at: 4526 6155 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1249 889 558 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3637 1537 Size: 275 1825 4090 Fragments arranged by size: 4090 1825 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2559 4120 1353 Size: 1206 1561 3423 Fragments arranged by size: 3423 1561 1206 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2991 4777 5151 5297 387 Size: 1064 138 1402 1786 374 146 1280 Fragments arranged by size: 1786 1402 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2118 2396 2118 Size: 278 5912 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2354 2514 2904 3054 4253 4850 Size: 393 416 306 160 390 150 1199 597 Cuts at: 4850 5637 5905 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1199 787 597 416 393 390 306 268 160 150 EcoNI CCTnn'n_nnAGG Cuts at: 2909 2984 2909 Size: 75 6115 EcoO109I rG'GnC_Cy Cuts at: 2945 2945 Size: 6190 EcoRI G'AATT_C Cuts at: 3499 3877 3499 Size: 378 5812 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2076 2588 3184 2076 Size: 512 596 5082 Fragments arranged by size: 5082 596 512 FspI TGC'GCA Cuts at: 3347 4696 3347 Size: 1349 4841 HaeII r_GCGC'y Cuts at: 1678 2927 3379 5337 1678 Size: 1249 452 1958 2531 Fragments arranged by size: 2531 1958 1249 452 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2275 2307 3260 3292 4831 Size: 32 135 32 703 32 953 32 1539 Cuts at: 4831 4863 4905 4937 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1539 953 703 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3534 3780 669 Size: 988 281 1596 246 3079 Fragments arranged by size: 3079 1596 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6190 HpaI GTT'AAC Cuts at: 3780 3780 Size: 6190 KasI G'GCGC_C Cuts at: 3375 3375 Size: 6190 KpnI G_GTAC'C Cuts at: 3515 3515 Size: 6190 MfeI C'AATT_G Cuts at: 3789 3789 Size: 6190 MluI A'CGCG_T Cuts at: 6066 6066 Size: 6190 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2506 5304 5488 1305 Size: 1201 2798 184 2007 Fragments arranged by size: 2798 2007 1201 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2390 10 Size: 54 1425 343 558 3810 Fragments arranged by size: 3810 1425 558 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2204 2214 4148 4507 4666 518 Size: 648 1038 10 1934 359 159 2042 Fragments arranged by size: 2042 1934 1038 648 359 159 10 MspA1I CmG'CkG Cuts at: 1606 3234 3606 4283 5431 5676 1606 Size: 1628 372 677 1148 245 2120 Fragments arranged by size: 2120 1628 1148 677 372 245 NaeI GCC'GGC Cuts at: 2540 2540 Size: 6190 NarI GG'CG_CC Cuts at: 3376 3376 Size: 6190 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4699 Fragments arranged by size: 4699 939 552 NdeI CA'TA_TG Cuts at: 387 2012 2211 387 Size: 1625 199 4366 Fragments arranged by size: 4366 1625 199 NgoMIV G'CCGG_C Cuts at: 2538 2538 Size: 6190 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6190 NotI GC'GGCC_GC Cuts at: 3637 3637 Size: 6190 NspI r_CATG'y Cuts at: 1132 1229 2352 2848 3371 3478 3548 5093 1132 Size: 97 1123 496 523 107 70 1545 2229 Fragments arranged by size: 2229 1545 1123 523 496 107 97 70 PasI CC'CwG_GG Cuts at: 1348 2986 1348 Size: 1638 4552 PciI A'CATG_T Cuts at: 5089 5089 Size: 6190 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6190 PfoI T'CCnGG_A Cuts at: 2025 2025 Size: 6190 PmeI GTTT'AAAC Cuts at: 3494 3494 Size: 6190 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4259 4291 5772 5804 4259 Size: 32 1481 32 4645 Fragments arranged by size: 4645 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2945 2945 Size: 6190 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6190 PsiI TTA'TAA Cuts at: 3760 3760 Size: 6190 PstI C_TGCA'G Cuts at: 830 2459 3233 3448 3542 830 Size: 1629 774 215 94 3478 Fragments arranged by size: 3478 1629 774 215 94 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6190 PvuII CAG'CTG Cuts at: 3234 3234 Size: 6190 SacI G_AGCT'C Cuts at: 2120 2398 2120 Size: 278 5912 SalI G'TCGA_C Cuts at: 1936 3532 1936 Size: 1596 4594 SapI GCTCTTCn'nnn_ Cuts at: 2559 2559 Size: 6190 SbfI CC_TGCA'GG Cuts at: 2459 3542 2459 Size: 1083 5107 ScaI AGT'ACT Cuts at: 4438 6150 4438 Size: 1712 4478 SfcI C'TryA_G Cuts at: 826 1045 2455 3229 3444 3538 4673 5354 5545 Size: 219 1410 774 215 94 1135 681 191 Cuts at: 5545 826 Size: 1471 Fragments arranged by size: 1471 1410 1135 774 681 219 215 191 94 SfoI GGC'GCC Cuts at: 3377 3377 Size: 6190 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6190 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6190 SmaI CCC'GGG Cuts at: 1872 3456 3517 1872 Size: 1584 61 4545 Fragments arranged by size: 4545 1584 61 SmlI C'TyrA_G Cuts at: 820 1017 1943 3185 4294 5195 5457 820 Size: 197 926 1242 1109 901 262 1553 Fragments arranged by size: 1553 1242 1109 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3981 493 Size: 3488 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6190 SphI G_CATG'C Cuts at: 1132 3371 3478 3548 1132 Size: 2239 107 70 3774 Fragments arranged by size: 3774 2239 107 70 SspI AAT'ATT Cuts at: 5 52 4114 5 Size: 47 4062 2081 Fragments arranged by size: 4062 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6190 StyI C'CwwG_G Cuts at: 513 1065 2004 2463 3629 513 Size: 552 939 459 1166 3074 Fragments arranged by size: 3074 1166 939 552 459 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3348 4217 4234 4387 4572 5017 325 Size: 1255 1768 869 17 153 185 445 1498 Fragments arranged by size: 1768 1498 1255 869 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2351 4436 6148 Size: 274 80 33 51 1443 374 2085 1712 Cuts at: 6148 96 Size: 138 Fragments arranged by size: 2085 1712 1443 374 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2937 2969 6150 6182 2937 Size: 32 3181 32 2945 Fragments arranged by size: 3181 2945 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6190 XbaI T'CTAG_A Cuts at: 3526 3526 Size: 6190 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2209 2209 Size: 6190 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6190 XmaI C'CCGG_G Cuts at: 1870 3454 3515 1870 Size: 1584 61 4545 Fragments arranged by size: 4545 1584 61 XmnI GAAnn'nnTTC Cuts at: 3221 4319 3221 Size: 1098 5092 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3998 276 Size: 53 83 186 936 2464 2468 Fragments arranged by size: 2468 2464 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI