Restriction map for
pFN21AB7266
(Circular) (Six-base) MAPSORT of: pFN21AB7266.seq Check: 4064 from: 1 to: 5737
pFN21AB7266 5737 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3547 278
Size: 53 83 186 936 2011 2468
Fragments arranged by size:
2468 2011 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3000 3080 1937
Size: 1063 80 4594
Fragments arranged by size:
4594 1063 80
Acc65I G'GTAC_C
Cuts at: 3058 3058
Size: 5737
AclI AA'CG_TT
Cuts at: 1563 3864 4237 1563
Size: 2301 373 3063
Fragments arranged by size:
3063 2301 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2288 2802 2827 3800 5184 5452 1632
Size: 656 514 25 973 1384 268 1917
Fragments arranged by size:
1917 1384 973 656 514 268 25
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5540
AflIII A'CryG_T
Cuts at: 1203 4636 5613 1203
Size: 3433 977 1327
Fragments arranged by size:
3433 1327 977
AgeI A'CCGG_T
Cuts at: 4568 4568
Size: 5737
AhdI GACnn_n'nnGTC
Cuts at: 4466 4466
Size: 5737
AleI CACnn'nnGTG
Cuts at: 1166 2559 1166
Size: 1393 4344
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2057 2790 1184
Size: 873 733 4131
Fragments arranged by size:
4131 873 733
AlwNI CAG_nnn'CTG
Cuts at: 2674 3480 5052 2674
Size: 806 1572 3359
Fragments arranged by size:
3359 1572 806
ApaLI G'TGCA_C
Cuts at: 3794 4950 3794
Size: 1156 4581
ApoI r'AATT_y
Cuts at: 1493 2147 2294 2960 3046 3272 3424 5533 1493
Size: 654 147 666 86 226 152 2109 1697
Fragments arranged by size:
2109 1697 666 654 226 152 147 86
AseI AT'TA_AT
Cuts at: 160 1032 4291 160
Size: 872 3259 1606
Fragments arranged by size:
3259 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5737
AvaI C'yCGr_G
Cuts at: 1870 1943 3062 1870
Size: 73 1119 4545
Fragments arranged by size:
4545 1119 73
BamHI G'GATC_C
Cuts at: 3067 3434 3067
Size: 367 5370
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3058 4513 618
Size: 325 217 627 1271 1455 1842
Fragments arranged by size:
1842 1455 1271 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3056 1391
Size: 1665 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2282 2398 928
Size: 971 383 116 4267
Fragments arranged by size:
4267 971 383 116
BbvCI CC'TCA_GC
Cuts at: 2818 2818
Size: 5737
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3928 3962 1903
Size: 34 1991 34 3678
Fragments arranged by size:
3678 1991 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2755 3629 4839 2755
Size: 874 1210 3653
Fragments arranged by size:
3653 1210 874
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5737
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4348 136
Size: 107 122 71 631 605 2676 1525
Fragments arranged by size:
2676 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5732 5732
Size: 5737
BlpI GC'TnA_GC
Cuts at: 3154 3154
Size: 5737
Bme1580I G_kGCm'C
Cuts at: 1790 2486 3798 4954 1790
Size: 696 1312 1156 2573
Fragments arranged by size:
2573 1312 1156 696
BmgBI CAC'GTC
Cuts at: 1318 2404 1318
Size: 1086 4651
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3468 4426 442
Size: 416 53 83 399 24 2051 958 1753
Fragments arranged by size:
2051 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5737
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4397 1239
Size: 3158 2579
Bpu10I CC'TnA_GC
Cuts at: 2567 2818 2567
Size: 251 5486
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2673 3862 4727 5025 2673
Size: 1189 865 298 3385
Fragments arranged by size:
3385 1189 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4400 882
Size: 617 2901 2219
Fragments arranged by size:
2901 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3528 493
Size: 711 2324 2702
Fragments arranged by size:
2702 2324 711
BsaBI GATnn'nnATC
Cuts at: 5731 5731
Size: 5737
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3544 3926 4561 275
Size: 53 83 186 936 2011 382 635 1451
Fragments arranged by size:
2011 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4170 4568 4842 4989 5400 4170
Size: 398 274 147 411 4507
Fragments arranged by size:
4507 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5512
Fragments arranged by size:
5512 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2175 1186
Size: 989 4748
BseYI C'CCAG_C
Cuts at: 4940 5436 4940
Size: 496 5241
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2522 2535 2961 1163
Size: 573 786 13 426 3939
Fragments arranged by size:
3939 786 573 426 13
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3187 3948 4976 1540
Size: 275 186 1186 761 1028 2301
Fragments arranged by size:
2301 1186 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3798 3883 4954 3798
Size: 85 1071 4581
Fragments arranged by size:
4581 1071 85
BsmI GAATG_Cn'
Cuts at: 3248 3341 3248
Size: 93 5644
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2486 3056 3798 3883 4954 1391
Size: 399 696 570 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 696 570 399 85
BspEI T'CCGG_A
Cuts at: 5400 5400
Size: 5737
BspHI T'CATG_A
Cuts at: 3626 5356 3626
Size: 1730 4007
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2423 2595 3092 844
Size: 1579 172 497 3489
Fragments arranged by size:
3489 1579 497 172
BsrBI CCG'CTC
Cuts at: 1394 1823 3624 1394
Size: 429 1801 3507
Fragments arranged by size:
3507 1801 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2574 2928 4232 4406 65
Size: 1183 1326 354 1304 174 1396
Fragments arranged by size:
1396 1326 1304 1183 354 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2051 4381 4568 1700
Size: 110 241 2330 187 2869
Fragments arranged by size:
2869 2330 241 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5737
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5737
BssSI C'ACGA_G
Cuts at: 2555 3797 4809 2555
Size: 1242 1012 3483
Fragments arranged by size:
3483 1242 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2939 794
Size: 2145 3592
BstBI TT'CG_AA
Cuts at: 2409 4541 2409
Size: 2132 3605
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5737
BstYI r'GATC_y
Cuts at: 1972 2684 2785 3067 3434 3819 3836 5288 5374
Size: 712 101 282 367 385 17 1452 86
Cuts at: 5374 5386 5732 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 712 385 367 346 282 101
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2881 2881
Size: 5737
BtgI C'CryG_G
Cuts at: 513 1065 2068 2538 2755 513
Size: 552 1003 470 217 3495
Fragments arranged by size:
3495 1003 552 470 217
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4998
Fragments arranged by size:
4998 683 56
BtsI GCAGTG_nn'
Cuts at: 3253 4047 4067 3253
Size: 794 20 4923
Fragments arranged by size:
4923 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5737
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5702
DraI TTT'AAA
Cuts at: 3041 3041
Size: 5737
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4744 809
Size: 507 3428 1802
Fragments arranged by size:
3428 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2167 2517 3184
Size: 54 1425 50 275 18 337 350 667
Cuts at: 3184 4073 5702 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 667 350 337 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3184 1537
Size: 275 1372 4090
Fragments arranged by size:
4090 1372 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2674 3667 1353
Size: 1321 993 3423
Fragments arranged by size:
3423 1321 993
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4324 4698 4844 387
Size: 1064 138 2735 374 146 1280
Fragments arranged by size:
2735 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2288 2802 2827 3800 4397 5184 5452
Size: 393 656 514 25 973 597 787 268
Cuts at: 5452 1239
Size: 1524
Fragments arranged by size:
1524 973 787 656 597 514 393 268
25
EcoRI G'AATT_C
Cuts at: 3046 3424 3046
Size: 378 5359
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2645 2645
Size: 5737
FspI TGC'GCA
Cuts at: 4243 4243
Size: 5737
HaeII r_GCGC'y
Cuts at: 1678 4884 1678
Size: 3206 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2091 2123 2636 2668 4378
Size: 32 135 32 519 32 513 32 1710
Cuts at: 4378 4410 4452 4484 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1710 519 513 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3081 3327 669
Size: 988 281 1143 246 3079
Fragments arranged by size:
3079 1143 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5737
HpaI GTT'AAC
Cuts at: 3327 3327
Size: 5737
KpnI G_GTAC'C
Cuts at: 3062 3062
Size: 5737
MfeI C'AATT_G
Cuts at: 3336 3336
Size: 5737
MluI A'CGCG_T
Cuts at: 5613 5613
Size: 5737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4851 5035 1305
Size: 3546 184 2007
Fragments arranged by size:
3546 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2169 2519 10
Size: 54 1425 343 337 350 3228
Fragments arranged by size:
3228 1425 350 343 337 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2559 3695 4054 4213 518
Size: 648 1393 1136 359 159 2042
Fragments arranged by size:
2042 1393 1136 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2780 3153 3830 4978 5223 1606
Size: 1174 373 677 1148 245 2120
Fragments arranged by size:
2120 1174 1148 677 373 245
NaeI GCC'GGC
Cuts at: 2053 2053
Size: 5737
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5185
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5737
NgoMIV G'CCGG_C
Cuts at: 2051 2051
Size: 5737
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5737
NotI GC'GGCC_GC
Cuts at: 3184 3184
Size: 5737
NspI r_CATG'y
Cuts at: 1132 1229 3095 4640 1132
Size: 97 1866 1545 2229
Fragments arranged by size:
2229 1866 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5737
PciI A'CATG_T
Cuts at: 4636 4636
Size: 5737
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5737
PfoI T'CCnGG_A
Cuts at: 2667 2667
Size: 5737
PmeI GTTT'AAAC
Cuts at: 3041 3041
Size: 5737
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3806 3838 5319 5351 3806
Size: 32 1481 32 4192
Fragments arranged by size:
4192 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5737
PsiI TTA'TAA
Cuts at: 2633 3307 2633
Size: 674 5063
PstI C_TGCA'G
Cuts at: 830 2592 3089 830
Size: 1762 497 3478
Fragments arranged by size:
3478 1762 497
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5737
PvuII CAG'CTG
Cuts at: 2780 2780
Size: 5737
SalI G'TCGA_C
Cuts at: 1936 3079 1936
Size: 1143 4594
SbfI CC_TGCA'GG
Cuts at: 2592 3089 2592
Size: 497 5240
ScaI AGT'ACT
Cuts at: 3985 5697 3985
Size: 1712 4025
SfcI C'TryA_G
Cuts at: 826 1045 2301 2588 3085 4220 4901 5092 826
Size: 219 1256 287 497 1135 681 191 1471
Fragments arranged by size:
1471 1256 1135 681 497 287 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5737
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5737
SmaI CCC'GGG
Cuts at: 1872 3064 1872
Size: 1192 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2688 3841 4742 5004 820
Size: 197 926 745 1153 901 262 1553
Fragments arranged by size:
1553 1153 926 901 745 262 197
SnaBI TAC'GTA
Cuts at: 493 3528 493
Size: 3035 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5737
SphI G_CATG'C
Cuts at: 1132 3095 1132
Size: 1963 3774
SspI AAT'ATT
Cuts at: 5 52 2640 2701 3661 5
Size: 47 2588 61 960 2081
Fragments arranged by size:
2588 2081 960 61 47
StuI AGG'CCT
Cuts at: 1517 2433 1517
Size: 916 4821
StyI C'CwwG_G
Cuts at: 513 1065 2976 3176 513
Size: 552 1911 200 3074
Fragments arranged by size:
3074 1911 552 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3038 3764 3781 3934 4119 4564 325
Size: 1255 1458 726 17 153 185 445 1498
Fragments arranged by size:
1498 1458 1255 726 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3983 5695 96
Size: 274 80 33 51 1443 2006 1712 138
Fragments arranged by size:
2006 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2371 2403 5697 5729 2371
Size: 32 3294 32 2379
Fragments arranged by size:
3294 2379 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5737
XbaI T'CTAG_A
Cuts at: 2152 3013 3073 2152
Size: 861 60 4816
Fragments arranged by size:
4816 861 60
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5737
XmaI C'CCGG_G
Cuts at: 1870 3062 1870
Size: 1192 4545
XmnI GAAnn'nnTTC
Cuts at: 3866 3866
Size: 5737
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3545 276
Size: 53 83 186 936 2011 2468
Fragments arranged by size:
2468 2011 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI