Restriction map for
pFN21AB7245
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BalI BamHI BbeI BcgI BciVI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I CelII CfoI ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MseI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SapI SbfI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I Tsp509I TthHB8I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 100 GTCTAC GT^[AC][GT]AC
AlwNI 224 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 864 CCTGAGG CC^T[ACGT]AGG
AspHI 64 GAGCTC G[AT]GC[AT]^C
AspI 31 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 59 CTCGGG C^[CT]CG[GA]G
AvaII 249 GGACC G^G[AT]CC
BanI 325 GGTGCC G^G[CT][GA]CC
BbrPI 18 CACGTG CAC^GTG
BbvCI 672 GCTGAGG CCTCAGC(-5/-2)
BsaAI 18 CACGTG [CT]AC^GT[GA]
BsiHKAI 64 GAGCTC G[AT]GC[AT]^C
BsoBI 59 CTCGGG C^[CT]CG[GA]G
BspMI 715 ACCTGC ACCTGC(4/8)
BsrDI 369 CATTGC GCAATG(2/0)
BsrFI 911 ACCGGC [GA]^CCGG[CT]
Bsu36I 864 CCTGAGG CC^T[ACGT]AGG
Cfr10I 911 ACCGGC [GA]^CCGG[CT]
DsaI 329 CCATGG C^C[GA][CT]GG
EaeI 819 CGGCCA [CT]^GGCC[GA]
EarI 219 CTCTTC CTCTTC(1/4)
Eco81I 864 CCTGAGG CC^T[ACGT]AGG
EcoT14I 329 CCATGG C^C[AT][AT]GG
HindIII 805 AAGCTT A^AGCTT
Ksp632I 219 CTCTTC CTCTTC(1/4)
MaeII 19 ACGT A^CGT
MspA1I 475 CAGCTG C[AC]G^C[GT]G
MwoI 489 GCTCCCACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 329 CCATGG C^CATGG
NspI 878 ACATGC [GA]CATG^[CT]
PflFI 31 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 312 CCACTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 57 GACTC GAGTC(4/5)
PmaCI 18 CACGTG CAC^GTG
PmlI 18 CACGTG CAC^GTG
PvuII 475 CAGCTG CAG^CTG
SacI 64 GAGCTC GAGCT^C
ScaI 549 AGTACT AGT^ACT
SmlI 592 CTCAAG C^T[CT][GA]AG
StyI 329 CCATGG C^C[AT][AT]GG
TfiI 154 GATTC G^A[AT]TC
Tth111I 31 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 312 CCACTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 236 CCAAAATGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AciI 363 CCGC CCGC(-3/-1)
390 CCGC
AfaI 177 GTAC GT^AC
550 GTAC
BanII 46 GGGCTC G[GA]GC[CT]^C
64 GAGCTC
BbsI 37 GTCTTC GAAGAC(2/6)
334 GTCTTC
BcnI 380 CCCGG CC^[GC]GG
776 CCCGG
BfaI 205 CTAG C^TAG
244 CTAG
BglII 299 AGATCT A^GATCT
596 AGATCT
BmyI 46 GGGCTC G[AGT]GC[ACT]^C
64 GAGCTC
BpmI 67 CTCCAG CTGGAG(16/14)
583 CTGGAG
BpuAI 37 GTCTTC GAAGAC(2/6)
334 GTCTTC
BseRI 253 CTCCTC GAGGAG(10/8)
637 CTCCTC
BsgI 564 CTGCAC GTGCAG(16/14)
717 CTGCAC
BsmAI 555 GAGAC GTCTC(1/5)
906 GAGAC
Bsp1286I 46 GGGCTC G[AGT]GC[ACT]^C
64 GAGCTC
BspHI 404 TCATGA T^CATGA
830 TCATGA
BstYI 299 AGATCT [GA]^GATC[CT]
596 AGATCT
HinfI 57 GACTC G^A[ACGT]TC
154 GATTC
MaeI 205 CTAG C^TAG
244 CTAG
MflI 299 AGATCT [GA]^GATC[CT]
596 AGATCT
NciI 380 CCCGG CC^[GC]GG
776 CCCGG
RcaI 404 TCATGA T^CATGA
830 TCATGA
RsaI 177 GTAC GT^AC
550 GTAC
SfaNI 486 GATGC GCATC(5/9)
669 GATGC
XhoII 299 AGATCT [GA]^GATC[CT]
596 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AluI BbvI BsaJI BsiYI BslI
BssKI BstF5I BstNI BstXI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI MaeIII MboI MboII MnlI
MslI MspI MvaI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TseI
Tsp45I TspRI