Restriction map for
pFN21AB7245
(Linear) (Six-base) MAPSORT of: KIBB7245.seq Check: 2218 from: 1 to: 921
KIBB7245.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 724 921
Size: 724 197
AccI GT'mk_AC
Cuts at: 0 101 921
Size: 101 820
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 921
Size: 206 715
AleI CACnn'nnGTG
Cuts at: 0 445 921
Size: 445 476
AlwNI CAG_nnn'CTG
Cuts at: 0 229 921
Size: 229 692
AvaI C'yCGr_G
Cuts at: 0 59 921
Size: 59 862
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 8 41 921
Size: 8 33 880
Fragments arranged by size:
880 33 8
BanI G'GyrC_C
Cuts at: 0 325 921
Size: 325 596
BanII G_rGCy'C
Cuts at: 0 50 68 921
Size: 50 18 853
Fragments arranged by size:
853 50 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 30 327 921
Size: 30 297 594
Fragments arranged by size:
594 297 30
BbvCI CC'TCA_GC
Cuts at: 0 673 921
Size: 673 248
BglII A'GATC_T
Cuts at: 0 299 596 921
Size: 299 297 325
Fragments arranged by size:
325 299 297
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 604 921
Size: 52 552 317
Fragments arranged by size:
552 317 52
Bpu10I CC'TnA_GC
Cuts at: 0 673 921
Size: 673 248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 921
Size: 577 344
BsaAI yAC'GTr
Cuts at: 0 20 921
Size: 20 901
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 443 473 921
Size: 443 30 448
Fragments arranged by size:
448 443 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 244 628 921
Size: 244 384 293
Fragments arranged by size:
384 293 244
BseYI C'CCAG_C
Cuts at: 0 158 921
Size: 158 763
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 549 702 921
Size: 549 153 219
Fragments arranged by size:
549 219 153
BsiHKAI G_wGCw'C
Cuts at: 0 68 921
Size: 68 853
Bsp1286I G_dGCh'C
Cuts at: 0 50 68 921
Size: 50 18 853
Fragments arranged by size:
853 50 18
BspHI T'CATG_A
Cuts at: 0 404 830 921
Size: 404 426 91
Fragments arranged by size:
426 404 91
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 724 921
Size: 724 197
BsrDI GCAATG_nn'
Cuts at: 0 368 921
Size: 368 553
BsrFI r'CCGG_y
Cuts at: 0 911 921
Size: 911 10
BstXI CCAn_nnnn'nTGG
Cuts at: 0 117 447 519 921
Size: 117 330 72 402
Fragments arranged by size:
402 330 117 72
BstYI r'GATC_y
Cuts at: 0 299 596 921
Size: 299 297 325
Fragments arranged by size:
325 299 297
Bsu36I CC'TnA_GG
Cuts at: 0 865 921
Size: 865 56
BtgI C'CryG_G
Cuts at: 0 329 921
Size: 329 592
BtsI GCAGTG_nn'
Cuts at: 0 227 493 577 896 921
Size: 227 266 84 319 25
Fragments arranged by size:
319 266 227 84 25
EaeI y'GGCC_r
Cuts at: 0 819 921
Size: 819 102
EarI CTCTTCn'nnn_
Cuts at: 0 225 921
Size: 225 696
EcoICRI GAG'CTC
Cuts at: 0 66 921
Size: 66 855
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 206 604 921
Size: 52 154 398 317
Fragments arranged by size:
398 317 154 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 241 273 443 475 666 698 921
Size: 241 32 170 32 191 32 223
Fragments arranged by size:
241 223 191 170 32 32 32
HindIII A'AGCT_T
Cuts at: 0 805 921
Size: 805 116
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 626 921
Size: 626 295
MslI CAynn'nnrTG
Cuts at: 0 115 142 172 403 445 664 733 829
Size: 115 27 30 231 42 219 69 96
Cuts at: 829 921
Size: 92
Fragments arranged by size:
231 219 115 96 92 69 42 30
27
MspA1I CmG'CkG
Cuts at: 0 477 921
Size: 477 444
NcoI C'CATG_G
Cuts at: 0 329 921
Size: 329 592
NspI r_CATG'y
Cuts at: 0 882 921
Size: 882 39
PflMI CCAn_nnn'nTGG
Cuts at: 0 318 921
Size: 318 603
PmlI CAC'GTG
Cuts at: 0 20 921
Size: 20 901
PvuII CAG'CTG
Cuts at: 0 477 921
Size: 477 444
SacI G_AGCT'C
Cuts at: 0 68 921
Size: 68 853
ScaI AGT'ACT
Cuts at: 0 551 921
Size: 551 370
SmlI C'TyrA_G
Cuts at: 0 592 921
Size: 592 329
StyI C'CwwG_G
Cuts at: 0 329 921
Size: 329 592
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 186 411 435 739 921
Size: 186 225 24 304 182
Fragments arranged by size:
304 225 186 182 24
TatI w'GTAC_w
Cuts at: 0 176 549 921
Size: 176 373 372
Fragments arranged by size:
373 372 176
Tth111I GACn'n_nGTC
Cuts at: 0 34 921
Size: 34 887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 243 921
Size: 243 678
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI AvaI BaeI BanI
BanII BbsI BbvCI BglII BpmI Bpu10I BpuEI BsaAI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI
BsrDI BsrFI BstXI BstYI Bsu36I BtgI BtsI EaeI
EarI EcoICRI Eco57MI Hin4I HindIII MmeI MslI MspA1I
NcoI NspI PflMI PmlI PvuII SacI ScaI SmlI
StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BamHI BbeI BcgI BciVI BclI BfrBI BglI
BlpI Bme1580I BmgBI BmrI BmtI BplI BsaI BsaBI
BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BsrBI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SapI SbfI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI XbaI XhoI XmaI XmnI ZraI