(Circular) (Six-base) MAPSORT of: pFN21AB7235.seq Check: 5691 from: 1 to: 6610 pFN21AB7235 6610 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4420 278 Size: 53 83 186 936 2884 2468 Fragments arranged by size: 2884 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3953 1937 Size: 2016 4594 Acc65I G'GTAC_C Cuts at: 2985 3931 2985 Size: 946 5664 AclI AA'CG_TT Cuts at: 1563 4737 5110 1563 Size: 3174 373 3063 Fragments arranged by size: 3174 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2138 2438 2682 2912 4673 6057 6325 1632 Size: 506 300 244 230 1761 1384 268 1917 Fragments arranged by size: 1917 1761 1384 506 300 268 244 230 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6413 AflIII A'CryG_T Cuts at: 1203 2277 5509 6486 1203 Size: 1074 3232 977 1327 Fragments arranged by size: 3232 1327 1074 977 AgeI A'CCGG_T Cuts at: 5441 5441 Size: 6610 AhdI GACnn_n'nnGTC Cuts at: 5339 5339 Size: 6610 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6610 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2986 3573 1184 Size: 1802 587 4221 Fragments arranged by size: 4221 1802 587 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3687 3719 3687 Size: 32 6578 AlwNI CAG_nnn'CTG Cuts at: 2200 2615 4353 5925 2200 Size: 415 1738 1572 2885 Fragments arranged by size: 2885 1738 1572 415 ApaI G_GGCC'C Cuts at: 2020 3337 2020 Size: 1317 5293 ApaLI G'TGCA_C Cuts at: 4667 5823 4667 Size: 1156 5454 ApoI r'AATT_y Cuts at: 1493 3919 4145 4297 6406 1493 Size: 2426 226 152 2109 1697 Fragments arranged by size: 2426 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5164 160 Size: 872 4132 1606 Fragments arranged by size: 4132 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6610 AvaI C'yCGr_G Cuts at: 1870 1943 3118 3329 3336 3935 1870 Size: 73 1175 211 7 599 4545 Fragments arranged by size: 4545 1175 599 211 73 7 AvrII C'CTAG_G Cuts at: 3619 3619 Size: 6610 BamHI G'GATC_C Cuts at: 2813 3156 3940 4307 2813 Size: 343 784 367 5116 Fragments arranged by size: 5116 784 367 343 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2985 3931 5386 618 Size: 325 217 627 1198 946 1455 1842 Fragments arranged by size: 1842 1455 1198 946 627 325 217 BanII G_rGCy'C Cuts at: 1391 2020 2115 3146 3337 3431 3656 3929 1391 Size: 629 95 1031 191 94 225 273 4072 Fragments arranged by size: 4072 1031 629 273 225 191 95 94 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5639 BbvCI CC'TCA_GC Cuts at: 3515 3542 3515 Size: 27 6583 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2262 2296 4801 4835 1903 Size: 34 50 34 241 34 2505 34 3678 Fragments arranged by size: 3678 2505 241 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4502 5712 4502 Size: 1210 5400 BclI T'GATC_A Cuts at: 1548 1548 Size: 6610 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5221 136 Size: 107 122 71 631 605 3549 1525 Fragments arranged by size: 3549 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3204 6605 3204 Size: 3401 3209 BlpI GC'TnA_GC Cuts at: 2640 4027 2640 Size: 1387 5223 Bme1580I G_kGCm'C Cuts at: 1790 2020 2965 3337 3827 4671 5827 1790 Size: 230 945 372 490 844 1156 2573 Fragments arranged by size: 2573 1156 945 844 490 372 230 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6610 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2175 2406 4341 Size: 416 53 83 399 24 758 231 1935 Cuts at: 4341 5299 442 Size: 958 1753 Fragments arranged by size: 1935 1753 958 758 416 399 231 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2934 1056 Size: 1878 4732 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2463 2726 2868 3193 3217 3338 3371 3445 Size: 1224 263 142 325 24 121 33 74 Cuts at: 3445 5270 1239 Size: 1825 2579 Fragments arranged by size: 2579 1825 1224 325 263 142 121 74 33 24 Bpu10I CC'TnA_GC Cuts at: 3515 3542 3650 3515 Size: 27 108 6475 Fragments arranged by size: 6475 108 27 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4735 5600 5898 4735 Size: 865 298 5447 Fragments arranged by size: 5447 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3257 3449 3461 5273 882 Size: 617 1758 192 12 1812 2219 Fragments arranged by size: 2219 1812 1758 617 192 12 BsaAI yAC'GTr Cuts at: 493 1204 2278 4401 493 Size: 711 1074 2123 2702 Fragments arranged by size: 2702 2123 1074 711 BsaBI GATnn'nnATC Cuts at: 6604 6604 Size: 6610 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4417 4799 5434 275 Size: 53 83 186 936 2884 382 635 1451 Fragments arranged by size: 2884 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5043 5441 5715 5862 6273 5043 Size: 398 274 147 411 5380 Fragments arranged by size: 5380 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2841 2871 3128 3158 3687 Size: 30 165 30 1438 30 257 30 529 Cuts at: 3687 3717 1178 Size: 30 4071 Fragments arranged by size: 4071 1438 529 257 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 2483 3354 1186 Size: 847 450 871 4442 Fragments arranged by size: 4442 871 847 450 BseYI C'CCAG_C Cuts at: 2463 3508 3698 5813 6309 2463 Size: 1045 190 2115 496 2764 Fragments arranged by size: 2764 2115 1045 496 190 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2309 3543 3593 1163 Size: 573 573 1234 50 4180 Fragments arranged by size: 4180 1234 573 573 50 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4060 4821 5849 1540 Size: 275 186 2059 761 1028 2301 Fragments arranged by size: 2301 2059 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2641 3431 4671 4756 5827 2641 Size: 790 1240 85 1071 3424 Fragments arranged by size: 3424 1240 1071 790 85 BsmI GAATG_Cn' Cuts at: 4121 4214 4121 Size: 93 6517 BsmBI CGTCTCn'nnnn_ Cuts at: 2315 2315 Size: 6610 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2020 2115 2641 2965 3146 3337 3431 Size: 399 230 95 526 324 181 191 94 Cuts at: 3431 3656 3827 3929 4671 4756 5827 1391 Size: 225 171 102 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 526 399 324 230 225 191 181 171 102 95 94 85 BspEI T'CCGG_A Cuts at: 6273 6273 Size: 6610 BspHI T'CATG_A Cuts at: 4499 6229 4499 Size: 1730 4880 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2588 2850 3615 3965 844 Size: 1744 262 765 350 3489 Fragments arranged by size: 3489 1744 765 350 262 BsrBI CCG'CTC Cuts at: 1394 1823 3494 4497 1394 Size: 429 1671 1003 3507 Fragments arranged by size: 3507 1671 1003 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2879 3864 5105 5279 65 Size: 1183 1631 985 1241 174 1396 Fragments arranged by size: 1631 1396 1241 1183 985 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2401 5254 5441 1700 Size: 110 591 2853 187 2869 Fragments arranged by size: 2869 2853 591 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6610 BssHII G'CGCG_C Cuts at: 1925 3388 1925 Size: 1463 5147 BssSI C'ACGA_G Cuts at: 3430 4670 5682 3430 Size: 1240 1012 4358 Fragments arranged by size: 4358 1240 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3505 794 Size: 2711 3899 BstBI TT'CG_AA Cuts at: 5414 5414 Size: 6610 BstEII G'GTnAC_C Cuts at: 1184 2029 3106 3487 1184 Size: 845 1077 381 4307 Fragments arranged by size: 4307 1077 845 381 BstXI CCAn_nnnn'nTGG Cuts at: 3249 3249 Size: 6610 BstYI r'GATC_y Cuts at: 1972 2527 2790 2813 3156 3204 3477 3940 4307 Size: 555 263 23 343 48 273 463 367 Cuts at: 4307 4692 4709 6161 6247 6259 6605 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 555 463 385 367 346 343 273 263 86 48 23 17 12 Bsu36I CC'TnA_GG Cuts at: 2498 3632 2498 Size: 1134 5476 BtgI C'CryG_G Cuts at: 513 1065 2213 2427 2550 3831 513 Size: 552 1148 214 123 1281 3292 Fragments arranged by size: 3292 1281 1148 552 214 123 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5871 Fragments arranged by size: 5871 683 56 BtsI GCAGTG_nn' Cuts at: 2862 3639 4126 4920 4940 2862 Size: 777 487 794 20 4532 Fragments arranged by size: 4532 794 777 487 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6610 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3432 3467 3847 3882 529 Size: 35 2868 35 380 35 3257 Fragments arranged by size: 3257 2868 380 35 35 35 DraI TTT'AAA Cuts at: 3914 3914 Size: 6610 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5617 809 Size: 507 4301 1802 Fragments arranged by size: 4301 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4057 4946 6575 Size: 54 1425 50 275 18 2227 889 1629 Cuts at: 6575 8 Size: 43 Fragments arranged by size: 2227 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4057 1537 Size: 275 2245 4090 Fragments arranged by size: 4090 2245 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2027 2163 2318 3003 3009 4540 1353 Size: 674 136 155 685 6 1531 3423 Fragments arranged by size: 3423 1531 685 674 155 136 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5197 5571 5717 387 Size: 1064 138 3608 374 146 1280 Fragments arranged by size: 3608 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3429 3429 Size: 6610 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2138 2438 2463 2682 2726 2868 2912 Size: 393 506 300 25 219 44 142 44 Cuts at: 2912 3193 3217 3338 3371 3445 4673 5270 6057 Size: 281 24 121 33 74 1228 597 787 Cuts at: 6057 6325 1239 Size: 268 1524 Fragments arranged by size: 1524 1228 787 597 506 393 300 281 268 219 142 121 74 44 44 33 25 24 EcoNI CCTnn'n_nnAGG Cuts at: 3101 3730 3101 Size: 629 5981 EcoO109I rG'GnC_Cy Cuts at: 2017 2143 3333 3345 3419 3616 3846 2017 Size: 126 1190 12 74 197 230 4781 Fragments arranged by size: 4781 1190 230 197 126 74 12 EcoRI G'AATT_C Cuts at: 3919 4297 3919 Size: 378 6232 FspI TGC'GCA Cuts at: 2774 5116 2774 Size: 2342 4268 HaeII r_GCGC'y Cuts at: 1678 2180 3522 5757 1678 Size: 502 1342 2235 2531 Fragments arranged by size: 2531 2235 1342 502 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2350 2382 2777 2809 3256 Size: 32 135 32 778 32 395 32 447 Cuts at: 3256 3288 3830 3862 5251 5283 5325 5357 1373 Size: 32 542 32 1389 32 42 32 2626 Fragments arranged by size: 2626 1389 778 542 447 395 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3954 4200 669 Size: 988 281 2016 246 3079 Fragments arranged by size: 3079 2016 988 281 246 HindIII A'AGCT_T Cuts at: 748 2694 748 Size: 1946 4664 HpaI GTT'AAC Cuts at: 4200 4200 Size: 6610 KpnI G_GTAC'C Cuts at: 2989 3935 2989 Size: 946 5664 MfeI C'AATT_G Cuts at: 4209 4209 Size: 6610 MluI A'CGCG_T Cuts at: 6486 6486 Size: 6610 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3417 5724 5908 1305 Size: 2112 2307 184 2007 Fragments arranged by size: 2307 2112 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4788 Fragments arranged by size: 4788 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3098 4568 4927 5086 518 Size: 648 1932 1470 359 159 2042 Fragments arranged by size: 2042 1932 1470 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2197 2215 2323 2463 2563 2770 3385 4026 Size: 591 18 108 140 100 207 615 641 Cuts at: 4026 4703 5851 6096 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 641 615 591 245 207 140 108 100 18 NaeI GCC'GGC Cuts at: 2403 2403 Size: 6610 NcoI C'CATG_G Cuts at: 513 1065 2427 3831 513 Size: 552 1362 1404 3292 Fragments arranged by size: 3292 1404 1362 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6610 NgoMIV G'CCGG_C Cuts at: 2401 2401 Size: 6610 NheI G'CTAG_C Cuts at: 1052 2930 1052 Size: 1878 4732 NotI GC'GGCC_GC Cuts at: 4057 4057 Size: 6610 NspI r_CATG'y Cuts at: 1132 1229 2257 3584 3968 5513 1132 Size: 97 1028 1327 384 1545 2229 Fragments arranged by size: 2229 1545 1327 1028 384 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6610 PciI A'CATG_T Cuts at: 5509 5509 Size: 6610 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6610 PfoI T'CCnGG_A Cuts at: 3028 3028 Size: 6610 PmeI GTTT'AAAC Cuts at: 3914 3914 Size: 6610 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3687 3719 4679 4711 6192 6224 3687 Size: 32 960 32 1481 32 4073 Fragments arranged by size: 4073 1481 960 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2143 3345 3419 3616 3846 2143 Size: 1202 74 197 230 4907 Fragments arranged by size: 4907 1202 230 197 74 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6610 PsiI TTA'TAA Cuts at: 4180 4180 Size: 6610 PspOMI G'GGCC_C Cuts at: 2016 3333 2016 Size: 1317 5293 PstI C_TGCA'G Cuts at: 830 2598 2769 3646 3962 830 Size: 1768 171 877 316 3478 Fragments arranged by size: 3478 1768 877 316 171 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6610 PvuII CAG'CTG Cuts at: 2197 2463 2563 2770 3385 2197 Size: 266 100 207 615 5422 Fragments arranged by size: 5422 615 266 207 100 SacI G_AGCT'C Cuts at: 3431 3431 Size: 6610 SacII CC_GC'GG Cuts at: 2216 2216 Size: 6610 SalI G'TCGA_C Cuts at: 1936 3952 1936 Size: 2016 4594 SanDI GG'GwC_CC Cuts at: 3345 3345 Size: 6610 SbfI CC_TGCA'GG Cuts at: 3962 3962 Size: 6610 ScaI AGT'ACT Cuts at: 4858 6570 4858 Size: 1712 4898 SfcI C'TryA_G Cuts at: 826 1045 2594 2765 3035 3565 3642 3958 5093 Size: 219 1549 171 270 530 77 316 1135 Cuts at: 5093 5774 5965 826 Size: 681 191 1471 Fragments arranged by size: 1549 1471 1135 681 530 316 270 219 191 171 77 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6610 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6610 SmaI CCC'GGG Cuts at: 1872 3120 3937 1872 Size: 1248 817 4545 Fragments arranged by size: 4545 1248 817 SmlI C'TyrA_G Cuts at: 820 1017 1943 4714 5615 5877 820 Size: 197 926 2771 901 262 1553 Fragments arranged by size: 2771 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4401 493 Size: 3908 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6610 SphI G_CATG'C Cuts at: 1132 2257 3584 3968 1132 Size: 1125 1327 384 3774 Fragments arranged by size: 3774 1327 1125 384 SspI AAT'ATT Cuts at: 5 52 4534 5 Size: 47 4482 2081 Fragments arranged by size: 4482 2081 47 StuI AGG'CCT Cuts at: 1517 2397 2602 1517 Size: 880 205 5525 Fragments arranged by size: 5525 880 205 StyI C'CwwG_G Cuts at: 513 1065 2427 2883 2938 3415 3483 3619 3660 Size: 552 1362 456 55 477 68 136 41 Cuts at: 3660 3831 4049 513 Size: 171 218 3074 Fragments arranged by size: 3074 1362 552 477 456 218 171 136 68 55 41 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2323 2420 3255 3844 3875 4637 4654 Size: 1255 743 97 835 589 31 762 17 Cuts at: 4654 4807 4992 5437 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 835 762 743 589 445 185 153 97 31 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4856 6568 96 Size: 274 80 33 51 1443 2879 1712 138 Fragments arranged by size: 2879 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2841 2873 3215 3230 3247 3262 6570 6602 2841 Size: 32 342 15 17 15 3308 32 2849 Fragments arranged by size: 3308 2849 342 32 32 17 15 15 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6610 XbaI T'CTAG_A Cuts at: 3946 3946 Size: 6610 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3192 3217 3894 3192 Size: 25 677 5908 Fragments arranged by size: 5908 677 25 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6610 XmaI C'CCGG_G Cuts at: 1870 3118 3935 1870 Size: 1248 817 4545 Fragments arranged by size: 4545 1248 817 XmnI GAAnn'nnTTC Cuts at: 2123 2345 4739 2123 Size: 222 2394 3994 Fragments arranged by size: 3994 2394 222 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4418 276 Size: 53 83 186 936 2884 2468 Fragments arranged by size: 2884 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SapI SexAI SfiI SfoI SrfI SwaI