Restriction map for
pFN21AB7232
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AclI Alw44I
AlwI AlwNI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspHI AvrII
BamHI BbeI BbrPI BciVI BglI
BglII BlnI BpmI BsaBI BsiEI
BsiHKAI BsiWI BsmBI Bsp1407I BspDI
BspHI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I ClaI CpoI DraI
DraIII DrdI EagI EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI HaeII HindIII
KasI KpnI Ksp632I KspI MamI
MfeI MflI MluI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PleI PmaCI PmeI PmlI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI StuI
SwaI XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 68 enzymes) #
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AccIII 75 TCCGGA T^CCGGA
AcsI 553 AAATTC [GA]^AATT[CT]
AflII 874 CTTAAG C^TTAAG
AflIII 726 ACATGT A^C[GA][CT]GT
AgeI 290 ACCGGT A^CCGGT
AhdI 12 GACCCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1069 CCTAAGG CC^T[ACGT]AGG
ApoI 553 AAATTC [GA]^AATT[CT]
AseI 511 ATTAAT AT^TAAT
AsnI 511 ATTAAT AT^TAAT
AspEI 12 GACCCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 139 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 951 CCCGGG C^[CT]CG[GA]G
AviII 326 TGCGCA TGC^GCA
BalI 801 TGGCCA TGG^CCA
BanI 222 GGTGCC G^G[CT][GA]CC
BanII 270 GGGCTC G[GA]GC[CT]^C
BbsI 20 GTCTTC GAAGAC(2/6)
BbvCI 437 CCTCAGC CCTCAGC(-5/-2)
BcgI 89 GCAGGAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1002 TGATCA T^GATCA
BfrI 874 CTTAAG C^TTAAG
BlpI 171 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 171 GCTCAGC GC^T[ACGT]AGC
BpuAI 20 GTCTTC GAAGAC(2/6)
BsaAI 904 TACGTG [CT]AC^GT[GA]
BsaI 195 GAGACC GGTCTC(1/5)
BseAI 75 TCCGGA T^CCGGA
BsgI 650 CTGCAC GTGCAG(16/14)
BsoBI 951 CCCGGG C^[CT]CG[GA]G
BspEI 75 TCCGGA T^CCGGA
BspLU11I 726 ACATGT A^CATGT
BsrDI 785 CATTGC GCAATG(2/0)
BsrFI 290 ACCGGT [GA]^CCGG[CT]
BsrI 935 ACTGG ACTGG(1/-1)
BstXI 566 CCAGACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1069 CCTAAGG CC^T[ACGT]AGG
CelII 171 GCTCAGC GC^T[ACGT]AGC
Cfr10I 290 ACCGGT [GA]^CCGG[CT]
DpnII 1003 GATC ^GATC
Eam1105I 12 GACCCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1069 CCTAAGG CC^T[ACGT]AGG
EcoT14I 669 CCATGG C^C[AT][AT]GG
FbaI 1002 TGATCA T^GATCA
FspI 326 TGCGCA TGC^GCA
HpaI 1054 GTTAAC GTT^AAC
MboI 1003 GATC ^GATC
MluNI 801 TGGCCA TGG^CCA
MroI 75 TCCGGA T^CCGGA
MscI 801 TGGCCA TGG^CCA
NcoI 669 CCATGG C^CATGG
NdeII 1003 GATC ^GATC
PflFI 139 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 288 CCACCGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 290 ACCGGT A^CCGGT
PshAI 1038 GACGTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 511 ATTAAT AT^TAAT
Sau3AI 1003 GATC ^GATC
SexAI 580 ACCAGGT A^CC[AT]GGT
SmaI 951 CCCGGG CCC^GGG
SmlI 874 CTTAAG C^T[CT][GA]AG
SphI 1075 GCATGC GCATG^C
SspI 514 AATATT AAT^ATT
StyI 669 CCATGG C^C[AT][AT]GG
Tth111I 139 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 288 CCACCGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 951 CCCGGG C^CCGGG
XmaI 951 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 60 CGCG CG^CG
99 CGCG
AcyI 105 GGCGTC G[GA]^CG[CT]C
214 GACGCC
AfaI 903 GTAC GT^AC
1116 GTAC
BmyI 223 GTGCCC G[AGT]GC[ACT]^C
270 GGGCTC
BsaHI 105 GGCGTC G[GA]^CG[CT]C
214 GACGCC
BsaWI 75 TCCGGA [AT]^CCGG[AT]
290 ACCGGT
BseRI 424 GAGGAG GAGGAG(10/8)
700 GAGGAG
BsmI 597 GAATGC GAATGC(1/-1)
982 GAATGC
Bsp1286I 223 GTGCCC G[AGT]GC[ACT]^C
270 GGGCTC
BstF5I 125 CATCC GGATG(2/0)
820 CATCC
BstUI 60 CGCG CG^CG
99 CGCG
CfoI 59 GCGC GCG^C
327 GCGC
EaeI 5 TGGCCG [CT]^GGCC[GA]
801 TGGCCA
FokI 125 CATCC GGATG(9/13)
820 CATCC
HgaI 214 GACGC GACGC(5/10)
106 GCGTC
HhaI 59 GCGC GCG^C
327 GCGC
Hin1I 105 GGCGTC G[GA]^CG[CT]C
214 GACGCC
HinP1I 59 GCGC G^CGC
327 GCGC
HincII 1045 GTCAAC GT[CT]^[GA]AC
1054 GTTAAC
HindII 1045 GTCAAC GT[CT]^[GA]AC
1054 GTTAAC
MslI 751 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1161 CATGGTTGTG
MvnI 60 CGCG CG^CG
99 CGCG
PvuII 765 CAGCTG CAG^CTG
882 CAGCTG
RsaI 903 GTAC GT^AC
1116 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BfaI BsaJI BsiYI BslI BsmAI
BsmFI BspMI BssKI BstNI Cac8I
Cfr13I DdeI DraII DsaI EcoO109I
EcoRII FauI Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboII MnlI MseI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV NspI PpuMI Sau96I
ScrFI SfaNI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I