(Circular) (Six-base) MAPSORT of: pFN21AB7232.seq Check: 2074 from: 1 to: 5893 pFN21AB7232 5893 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2601 2601 Size: 5893 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3703 278 Size: 53 83 186 936 2167 2468 Fragments arranged by size: 2468 2167 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3236 1937 Size: 1299 4594 Acc65I G'GTAC_C Cuts at: 3214 3214 Size: 5893 AclI AA'CG_TT Cuts at: 1563 4020 4393 1563 Size: 2457 373 3063 Fragments arranged by size: 3063 2457 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2438 2902 3956 5340 5608 1632 Size: 806 464 1054 1384 268 1917 Fragments arranged by size: 1917 1384 1054 806 464 268 AflII C'TTAA_G Cuts at: 820 1017 2879 820 Size: 197 1862 3834 Fragments arranged by size: 3834 1862 197 AflIII A'CryG_T Cuts at: 1203 2731 4792 5769 1203 Size: 1528 2061 977 1327 Fragments arranged by size: 2061 1528 1327 977 AgeI A'CCGG_T Cuts at: 2295 4724 2295 Size: 2429 3464 AhdI GACnn_n'nnGTC Cuts at: 2022 4622 2022 Size: 2600 3293 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5893 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2768 1184 Size: 1584 4309 AlwNI CAG_nnn'CTG Cuts at: 3636 5208 3636 Size: 1572 4321 ApaLI G'TGCA_C Cuts at: 3950 5106 3950 Size: 1156 4737 ApoI r'AATT_y Cuts at: 1493 2558 3202 3428 3580 5689 1493 Size: 1065 644 226 152 2109 1697 Fragments arranged by size: 2109 1697 1065 644 226 152 AseI AT'TA_AT Cuts at: 160 1032 2517 4447 160 Size: 872 1485 1930 1606 Fragments arranged by size: 1930 1606 1485 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5893 AvaI C'yCGr_G Cuts at: 1870 1943 2956 3218 1870 Size: 73 1013 262 4545 Fragments arranged by size: 4545 1013 262 73 BamHI G'GATC_C Cuts at: 3223 3590 3223 Size: 367 5526 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2227 3214 4669 618 Size: 325 217 627 440 987 1455 1842 Fragments arranged by size: 1842 1455 987 627 440 325 217 BanII G_rGCy'C Cuts at: 1391 2279 3212 1391 Size: 888 933 4072 Fragments arranged by size: 4072 933 888 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2018 928 Size: 971 119 4803 Fragments arranged by size: 4803 971 119 BbvCI CC'TCA_GC Cuts at: 2443 2443 Size: 5893 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2083 2117 4084 4118 1903 Size: 34 146 34 1967 34 3678 Fragments arranged by size: 3678 1967 146 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3785 4995 3785 Size: 1210 4683 BclI T'GATC_A Cuts at: 1548 3007 1548 Size: 1459 4434 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4504 136 Size: 107 122 71 631 605 337 2495 1525 Fragments arranged by size: 2495 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5888 5888 Size: 5893 BlpI GC'TnA_GC Cuts at: 2177 3310 2177 Size: 1133 4760 Bme1580I G_kGCm'C Cuts at: 1790 2232 3954 5110 1790 Size: 442 1722 1156 2573 Fragments arranged by size: 2573 1722 1156 442 BmgBI CAC'GTC Cuts at: 1318 2926 1318 Size: 1608 4285 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3624 4582 442 Size: 416 53 83 399 24 2207 958 1753 Fragments arranged by size: 2207 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5893 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4553 1239 Size: 3314 2579 Bpu10I CC'TnA_GC Cuts at: 2153 2443 2153 Size: 290 5603 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4018 4883 5181 4018 Size: 865 298 4730 Fragments arranged by size: 4730 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2194 4556 882 Size: 617 695 2362 2219 Fragments arranged by size: 2362 2219 695 617 BsaAI yAC'GTr Cuts at: 493 1204 2911 3684 493 Size: 711 1707 773 2702 Fragments arranged by size: 2702 1707 773 711 BsaBI GATnn'nnATC Cuts at: 5887 5887 Size: 5893 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2111 2220 3700 4082 Size: 53 83 186 936 578 109 1480 382 Cuts at: 4082 4717 275 Size: 635 1451 Fragments arranged by size: 1480 1451 936 635 578 382 186 109 83 53 BsaWI w'CCGG_w Cuts at: 2080 2295 4326 4724 4998 5145 5556 2080 Size: 215 2031 398 274 147 411 2417 Fragments arranged by size: 2417 2031 411 398 274 215 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5668 Fragments arranged by size: 5668 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2444 2720 1186 Size: 1258 276 4359 Fragments arranged by size: 4359 1258 276 BseYI C'CCAG_C Cuts at: 2086 2133 2210 5096 5592 2086 Size: 47 77 2886 496 2387 Fragments arranged by size: 2886 2387 496 77 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2640 1163 Size: 573 904 4416 Fragments arranged by size: 4416 904 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3343 4104 5132 1540 Size: 275 186 1342 761 1028 2301 Fragments arranged by size: 2301 1342 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3954 4039 5110 3954 Size: 85 1071 4737 Fragments arranged by size: 4737 1071 85 BsmI GAATG_Cn' Cuts at: 2608 2993 3404 3497 2608 Size: 385 411 93 5004 Fragments arranged by size: 5004 411 385 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2232 2279 3212 3954 4039 5110 1391 Size: 399 442 47 933 742 85 1071 2174 Fragments arranged by size: 2174 1071 933 742 442 399 85 47 BspEI T'CCGG_A Cuts at: 2080 5556 2080 Size: 3476 2417 BspHI T'CATG_A Cuts at: 3782 5512 3782 Size: 1730 4163 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2548 2601 2736 3248 844 Size: 1704 53 135 512 3489 Fragments arranged by size: 3489 1704 512 135 53 BsrBI CCG'CTC Cuts at: 1394 1823 3780 1394 Size: 429 1957 3507 Fragments arranged by size: 3507 1957 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2789 4388 4562 65 Size: 1183 1541 1599 174 1396 Fragments arranged by size: 1599 1541 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2295 4537 4724 1700 Size: 110 485 2242 187 2869 Fragments arranged by size: 2869 2242 485 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5893 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5893 BssSI C'ACGA_G Cuts at: 3953 4965 3953 Size: 1012 4881 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5893 BstBI TT'CG_AA Cuts at: 4697 4697 Size: 5893 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5893 BstXI CCAn_nnnn'nTGG Cuts at: 2578 2578 Size: 5893 BstYI r'GATC_y Cuts at: 1972 3223 3590 3975 3992 5444 5530 5542 5888 Size: 1251 367 385 17 1452 86 12 346 Cuts at: 5888 1972 Size: 1977 Fragments arranged by size: 1977 1452 1251 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3075 3075 Size: 5893 BtgI C'CryG_G Cuts at: 513 1065 2004 2223 2424 2674 2980 513 Size: 552 939 219 201 250 306 3426 Fragments arranged by size: 3426 939 552 306 250 219 201 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5154 Fragments arranged by size: 5154 683 56 BtsI GCAGTG_nn' Cuts at: 3409 4203 4223 3409 Size: 794 20 5079 Fragments arranged by size: 5079 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5893 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5858 DraI TTT'AAA Cuts at: 3197 3197 Size: 5893 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4900 809 Size: 507 3584 1802 Fragments arranged by size: 3584 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2806 3340 Size: 54 1425 50 275 18 180 796 534 Cuts at: 3340 4229 5858 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 796 534 275 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3340 1537 Size: 275 1528 4090 Fragments arranged by size: 4090 1528 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3823 1353 Size: 2470 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4480 4854 5000 387 Size: 1064 138 2891 374 146 1280 Fragments arranged by size: 2891 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2438 2902 3956 4553 5340 5608 1239 Size: 393 806 464 1054 597 787 268 1524 Fragments arranged by size: 1524 1054 806 787 597 464 393 268 EcoO109I rG'GnC_Cy Cuts at: 2016 2050 2149 2016 Size: 34 99 5760 Fragments arranged by size: 5760 99 34 EcoRI G'AATT_C Cuts at: 3202 3580 3202 Size: 378 5515 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2766 2766 Size: 5893 FspI TGC'GCA Cuts at: 2333 4399 2333 Size: 2066 3827 HaeII r_GCGC'y Cuts at: 1678 5040 1678 Size: 3362 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2008 2040 2935 2967 4534 Size: 32 135 32 436 32 895 32 1567 Cuts at: 4534 4566 4608 4640 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1567 895 436 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3052 3061 3237 3483 669 Size: 988 281 1114 9 176 246 3079 Fragments arranged by size: 3079 1114 988 281 246 176 9 HindIII A'AGCT_T Cuts at: 748 748 Size: 5893 HpaI GTT'AAC Cuts at: 3061 3483 3061 Size: 422 5471 KpnI G_GTAC'C Cuts at: 3218 3218 Size: 5893 MfeI C'AATT_G Cuts at: 3492 3492 Size: 5893 MluI A'CGCG_T Cuts at: 5769 5769 Size: 5893 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2280 5007 5191 1305 Size: 975 2727 184 2007 Fragments arranged by size: 2727 2007 975 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2808 10 Size: 54 1425 343 976 3095 Fragments arranged by size: 3095 1425 976 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2760 3170 3851 4210 4369 518 Size: 648 1594 410 681 359 159 2042 Fragments arranged by size: 2042 1594 681 648 410 359 159 MspA1I CmG'CkG Cuts at: 1606 2022 2639 2772 2869 2889 3309 3986 5134 Size: 416 617 133 97 20 420 677 1148 Cuts at: 5134 5379 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 617 420 416 245 133 97 20 NcoI C'CATG_G Cuts at: 513 1065 2004 2674 513 Size: 552 939 670 3732 Fragments arranged by size: 3732 939 670 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5893 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5893 NotI GC'GGCC_GC Cuts at: 3340 3340 Size: 5893 NspI r_CATG'y Cuts at: 1132 1229 2185 2242 2735 3084 3251 4796 1132 Size: 97 956 57 493 349 167 1545 2229 Fragments arranged by size: 2229 1545 956 493 349 167 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5893 PciI A'CATG_T Cuts at: 2731 4792 2731 Size: 2061 3832 PflMI CCAn_nnn'nTGG Cuts at: 1108 2299 1108 Size: 1191 4702 PmeI GTTT'AAAC Cuts at: 3197 3197 Size: 5893 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3962 3994 5475 5507 3962 Size: 32 1481 32 4348 Fragments arranged by size: 4348 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2016 2050 2149 2016 Size: 34 99 5760 Fragments arranged by size: 5760 99 34 PshAI GACnn'nnGTC Cuts at: 1613 3047 1613 Size: 1434 4459 PsiI TTA'TAA Cuts at: 3463 3463 Size: 5893 PstI C_TGCA'G Cuts at: 830 3245 830 Size: 2415 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5893 PvuII CAG'CTG Cuts at: 2772 2889 2772 Size: 117 5776 SalI G'TCGA_C Cuts at: 1936 3235 1936 Size: 1299 4594 SanDI GG'GwC_CC Cuts at: 2016 2016 Size: 5893 SbfI CC_TGCA'GG Cuts at: 3245 3245 Size: 5893 ScaI AGT'ACT Cuts at: 4141 5853 4141 Size: 1712 4181 SexAI A'CCwGG_T Cuts at: 2585 2585 Size: 5893 SfcI C'TryA_G Cuts at: 826 1045 3241 4376 5057 5248 826 Size: 219 2196 1135 681 191 1471 Fragments arranged by size: 2196 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5893 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5893 SmaI CCC'GGG Cuts at: 1872 2958 3220 1872 Size: 1086 262 4545 Fragments arranged by size: 4545 1086 262 SmlI C'TyrA_G Cuts at: 820 1017 1943 2879 3997 4898 5160 820 Size: 197 926 936 1118 901 262 1553 Fragments arranged by size: 1553 1118 936 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3684 493 Size: 3191 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5893 SphI G_CATG'C Cuts at: 1132 3084 3251 1132 Size: 1952 167 3774 Fragments arranged by size: 3774 1952 167 SspI AAT'ATT Cuts at: 5 52 2521 3817 5 Size: 47 2469 1296 2081 Fragments arranged by size: 2469 2081 1296 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5893 StyI C'CwwG_G Cuts at: 513 1065 2004 2674 3332 513 Size: 552 939 670 658 3074 Fragments arranged by size: 3074 939 670 658 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3174 3920 3937 4090 4275 4720 325 Size: 1255 1594 746 17 153 185 445 1498 Fragments arranged by size: 1594 1498 1255 746 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4139 5851 96 Size: 274 80 33 51 1443 2162 1712 138 Fragments arranged by size: 2162 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2670 2702 5853 5885 2670 Size: 32 3151 32 2678 Fragments arranged by size: 3151 2678 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2147 1316 Size: 831 5062 XbaI T'CTAG_A Cuts at: 3229 3229 Size: 5893 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5893 XmaI C'CCGG_G Cuts at: 1870 2956 3218 1870 Size: 1086 262 4545 Fragments arranged by size: 4545 1086 262 XmnI GAAnn'nnTTC Cuts at: 4022 4022 Size: 5893 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3701 276 Size: 53 83 186 936 2167 2468 Fragments arranged by size: 2468 2167 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI