Restriction map for
pFN21AB7221
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccII AccIII AciI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbvCI
BcgI BciVI BcnI BfaI BfrI
BglI BglII BlnI BpmI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FokI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MaeI MamI MfeI
MluI MluNI MroI MscI MseI
MslI MspI MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StyI SwaI
TaqI Tru9I Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 176 GTCTAC GT^[AC][GT]AC
AclI 100 AACGTT AA^CGTT
AcsI 595 AAATTT [GA]^AATT[CT]
AfaI 369 GTAC GT^AC
AlwI 45 GATCC GGATC(4/5)
AlwNI 534 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 595 AAATTT [GA]^AATT[CT]
Asp700I 252 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 3 GAGCAC G[AT]GC[AT]^C
AvaII 459 GGTCC G^G[AT]CC
BanI 70 GGCACC G^G[CT][GA]CC
BbvI 473 GCAGC GCAGC(8/12)
BclI 354 TGATCA T^GATCA
BlpI 468 GCTGAGC GC^T[ACGT]AGC
BmyI 3 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 468 GCTGAGC GC^T[ACGT]AGC
BseRI 486 GAGGAG GAGGAG(10/8)
BsiHKAI 3 GAGCAC G[AT]GC[AT]^C
Bsp1286I 3 GAGCAC G[AGT]GC[ACT]^C
BsrDI 63 CATTGC GCAATG(2/0)
BstYI 44 AGATCC [GA]^GATC[CT]
CelII 468 GCTGAGC GC^T[ACGT]AGC
Cfr13I 459 GGTCC G^G[ACGT]CC
DraII 458 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 458 GGGTCCC [GA]G^G[ACGT]CC[CT]
FbaI 354 TGATCA T^GATCA
Fnu4HI 473 GCAGC GC^[ACGT]GC
ItaI 473 GCAGC GC^[ACGT]GC
MaeII 101 ACGT A^CGT
MflI 44 AGATCC [GA]^GATC[CT]
MspA1I 466 CAGCTG C[AC]G^C[GT]G
NlaIII 414 CATG CATG^
PflMI 378 CCAATGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 116 GAGTC GAGTC(4/5)
PpuMI 458 GGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 100 AACGTT AA^CGTT
PvuII 466 CAGCTG CAG^CTG
RsaI 369 GTAC GT^AC
Sau96I 459 GGTCC G^G[ACGT]CC
SmlI 40 CTTGAG C^T[CT][GA]AG
SspI 311 AATATT AAT^ATT
TfiI 21 GATTC G^A[AT]TC
TseI 473 GCAGC G^C[AT]GC
TspRI 534 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 378 CCAATGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 44 AGATCC [GA]^GATC[CT]
XmnI 252 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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BbsI 391 GAAGAC GAAGAC(2/6)
409 GAAGAC
BpuAI 391 GAAGAC GAAGAC(2/6)
409 GAAGAC
BsiYI 75 CCTATCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
378 CCAATGCTTGG
BslI 75 CCTATCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
378 CCAATGCTTGG
BsmAI 135 GAGAC GTCTC(1/5)
324 GAGAC
Cac8I 67 GCTGGC GC[ACGT]^[ACGT]GC
360 GCAAGC
DpnII 45 GATC ^GATC
355 GATC
EarI 113 GAAGAG CTCTTC(1/4)
237 GAAGAG
HaeIII 255 GGCC GG^CC
279 GGCC
HinfI 21 GATTC G^A[ACGT]TC
116 GAGTC
HphI 105 TCACC GGTGA(8/7)
119 TCACC
Ksp632I 113 GAAGAG CTCTTC(1/4)
237 GAAGAG
MboI 45 GATC ^GATC
355 GATC
NdeII 45 GATC ^GATC
355 GATC
Sau3AI 45 GATC ^GATC
355 GATC
SfcI 167 CTGTAG C^T[GA][CT]AG
274 CTACAG
StuI 254 AGGCCT AGG^CCT
278 AGGCCT
Tsp45I 118 GTCAC ^GT[GC]AC
536 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AluI BsaJI BsmFI BssKI BstNI
DdeI EcoRII MaeIII MboII MnlI
MvaI NlaIV ScrFI Tsp509I