Restriction map for
pFN21AB7200
##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AocI Aor51HI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeIII MamI MfeI
MluI MseI MunI MvnI NarI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tsp45I Tsp509I Tth111I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 923 TCCGGA T^CCGGA
AfaI 715 GTAC GT^AC
AlwI 455 GGATC GGATC(4/5)
Asp700I 615 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 464 TGGCCA TGG^CCA
BclI 383 TGATCA T^GATCA
BfaI 226 CTAG C^TAG
BsaWI 923 TCCGGA [AT]^CCGG[AT]
BseAI 923 TCCGGA T^CCGGA
BsmFI 874 GTCCC GGGAC(10/14)
BspEI 923 TCCGGA T^CCGGA
BsrFI 492 GCCGGC [GA]^CCGG[CT]
BstAPI 732 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 622 TTCGAA TT^CGAA
BstXI 774 CCACCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 492 GCCGGC [GA]^CCGG[CT]
EaeI 464 TGGCCA [CT]^GGCC[GA]
FbaI 383 TGATCA T^GATCA
HinfI 1167 GAGTC G^A[ACGT]TC
HphI 1019 TCACC GGTGA(8/7)
MaeI 226 CTAG C^TAG
MaeII 461 ACGT A^CGT
MluNI 464 TGGCCA TGG^CCA
MroI 923 TCCGGA T^CCGGA
MscI 464 TGGCCA TGG^CCA
MslI 1101 CACACGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 492 GCCGGC GCC^GGC
NcoI 945 CCATGG C^CATGG
NgoMIV 492 GCCGGC G^CCGGC
NspV 622 TTCGAA TT^CGAA
PleI 1167 GAGTC GAGTC(4/5)
RsaI 715 GTAC GT^AC
SacI 564 GAGCTC GAGCT^C
SapI 625 GAAGAGC GCTCTTC(1/4)
SfuI 622 TTCGAA TT^CGAA
StuI 13 AGGCCT AGG^CCT
XmnI 615 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Alw44I 358 GTGCAC G^TGCAC
524 GTGCAC
AlwNI 718 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
730 CAGCAGCTG
ApaLI 358 GTGCAC G^TGCAC
524 GTGCAC
AvaI 113 CCCGGG C^[CT]CG[GA]G
893 CCCGGG
BanI 237 GGCACC G^G[CT][GA]CC
929 GGCACC
BbsI 418 GTCTTC GAAGAC(2/6)
619 GTCTTC
BglI 763 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
772 GCCCACCTGGC
BglII 144 AGATCT A^GATCT
1085 AGATCT
BpuAI 418 GTCTTC GAAGAC(2/6)
619 GTCTTC
BsmAI 695 GTCTC GTCTC(1/5)
720 GTCTC
BsoBI 113 CCCGGG C^[CT]CG[GA]G
893 CCCGGG
BstYI 144 AGATCT [GA]^GATC[CT]
1085 AGATCT
DsaI 945 CCATGG C^C[GA][CT]GG
1220 CCGTGG
EarI 171 CTCTTC CTCTTC(1/4)
625 GAAGAG
FauI 155 GCGGG CCCGC(4/6)
1188 GCGGG
Ksp632I 171 CTCTTC CTCTTC(1/4)
625 GAAGAG
MflI 144 AGATCT [GA]^GATC[CT]
1085 AGATCT
NspI 1004 ACATGC [GA]CATG^[CT]
1106 GCATGT
PflMI 663 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
975 CCACAACATGG
PstI 205 CTGCAG CTGCA^G
352 CTGCAG
SfcI 205 CTGCAG C^T[GA][CT]AG
352 CTGCAG
SmaI 113 CCCGGG CCC^GGG
893 CCCGGG
SmlI 427 CTCAAG C^T[CT][GA]AG
643 CTCAAG
TaqI 150 TCGA T^CGA
623 TCGA
TthHB8I 150 TCGA T^CGA
623 TCGA
Van91I 663 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
975 CCACAACATGG
XhoII 144 AGATCT [GA]^GATC[CT]
1085 AGATCT
XmaCI 113 CCCGGG C^CCGGG
893 CCCGGG
XmaI 113 CCCGGG C^CCGGG
893 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI ApaI AspHI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HpaII
ItaI MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PvuII Sau3AI Sau96I
ScrFI SfaNI StyI TseI TspRI